Mutagenetix > Incidental Mutation

Incidental Mutation 'R1536:Or13c25'

169514

Institutional Source

Beutler Lab

Gene Symbol

Or13c25

Ensembl Gene

ENSMUSG00000051593

Gene Name

olfactory receptor family 13 subfamily C member 25

Synonyms

MOR262-7, GA_x6K02T2N78B-7084885-7085844, Olfr272

MMRRC Submission

039575-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R1536 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52910833-52911792 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 52911260 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 178 (V178A)

Ref Sequence

ENSEMBL: ENSMUSP00000149339 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051600] [ENSMUST00000107667] [ENSMUST00000213989]

AlphaFold

Q8VGA0

Predicted Effect

probably benign
Transcript: ENSMUST00000051600
AA Change: V178A

PolyPhen 2 Score 0.431 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000055721
Gene: ENSMUSG00000051593
AA Change: V178A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	314	1.6e-54	PFAM
Pfam:7tm_1	41	296	9.5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000107667
AA Change: V178A

PolyPhen 2 Score 0.431 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000103294
Gene: ENSMUSG00000051593
AA Change: V178A

Domain	Start	End	E-Value	Type
Pfam:7tm_1	39	294	9.5e-33	PFAM
Pfam:7tm_4	138	287	3.3e-42	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213989
AA Change: V178A

PolyPhen 2 Score 0.431 (Sensitivity: 0.89; Specificity: 0.90)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000217085

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 95.3%
20x: 89.5%

Validation Efficiency

98% (59/60)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310003L06Rik	A	T	5: 88,118,524 (GRCm39)	I3F	probably benign	Het
4930590J08Rik	A	G	6: 91,894,016 (GRCm39)	N211S	probably benign	Het
A2ml1	A	T	6: 128,524,196 (GRCm39)	Y1145*	probably null	Het
Abca7	A	G	10: 79,850,064 (GRCm39)	D1972G	probably benign	Het
Adamts19	A	T	18: 59,185,687 (GRCm39)	D1187V	probably damaging	Het
Adcy6	G	C	15: 98,497,888 (GRCm39)	I421M	probably damaging	Het
Afap1	C	A	5: 36,131,835 (GRCm39)	H387Q	probably damaging	Het
Atp8b1	C	T	18: 64,678,335 (GRCm39)	V854M	probably damaging	Het
Auts2	C	T	5: 131,516,302 (GRCm39)		probably benign	Het
Cbll1	T	C	12: 31,537,855 (GRCm39)	D300G	probably damaging	Het
Cd200r4	A	T	16: 44,653,412 (GRCm39)	T61S	possibly damaging	Het
Chmp4c	G	T	3: 10,454,744 (GRCm39)	V207L	probably benign	Het
Cntn5	T	C	9: 9,976,321 (GRCm39)	T413A	possibly damaging	Het
Cox7a2	T	A	9: 79,665,863 (GRCm39)		probably null	Het
Cwc27	A	G	13: 104,933,814 (GRCm39)	L236P	probably damaging	Het
Diaph1	A	G	18: 38,029,146 (GRCm39)		probably null	Het
Dst	T	A	1: 34,299,453 (GRCm39)		probably benign	Het
Ear1	T	A	14: 44,056,583 (GRCm39)	H95L	probably damaging	Het
Enpp1	T	A	10: 24,517,732 (GRCm39)	H898L	probably benign	Het
Entpd5	G	A	12: 84,429,069 (GRCm39)	R321*	probably null	Het
Ercc6l2	C	A	13: 63,972,685 (GRCm39)	N177K	possibly damaging	Het
Ergic1	T	C	17: 26,860,680 (GRCm39)		probably null	Het
Erich6	A	T	3: 58,534,019 (GRCm39)	I336N	probably benign	Het
Fmnl2	A	G	2: 52,995,549 (GRCm39)	E424G	probably damaging	Het
Galnt3	A	T	2: 65,914,550 (GRCm39)	D622E	probably damaging	Het
Gjd4	T	A	18: 9,280,569 (GRCm39)	T170S	probably damaging	Het
Gm5611	G	A	9: 16,941,903 (GRCm39)		noncoding transcript	Het
Gpc5	T	A	14: 115,636,662 (GRCm39)	N448K	probably benign	Het
Klra3	G	C	6: 130,310,107 (GRCm39)	R138G	probably benign	Het
Maco1	A	G	4: 134,531,818 (GRCm39)	V617A	probably damaging	Het
Man2b2	T	C	5: 36,978,271 (GRCm39)	T338A	probably benign	Het
Mbtps1	A	T	8: 120,272,864 (GRCm39)	S94T	probably benign	Het
Muc3a	A	T	5: 137,244,538 (GRCm39)	S205T	unknown	Het
Nav2	C	A	7: 49,195,682 (GRCm39)	D1019E	probably damaging	Het
Neurl4	T	A	11: 69,794,252 (GRCm39)	L236*	probably null	Het
Plcxd3	T	C	15: 4,546,093 (GRCm39)		probably benign	Het
Pprc1	T	C	19: 46,059,965 (GRCm39)		probably benign	Het
Prkaa2	T	A	4: 104,932,647 (GRCm39)	N67I	probably damaging	Het
Prom1	T	A	5: 44,175,695 (GRCm39)	Y508F	probably benign	Het
Prx	A	G	7: 27,216,683 (GRCm39)	M534V	probably damaging	Het
Rps6kc1	C	T	1: 190,603,965 (GRCm39)	R219Q	possibly damaging	Het
Sbf2	T	C	7: 109,977,250 (GRCm39)	Y628C	probably damaging	Het
Slc1a2	A	T	2: 102,607,855 (GRCm39)	D501V	probably benign	Het
Spata31	A	T	13: 65,069,196 (GRCm39)	Q448L	probably damaging	Het
Stk35	T	C	2: 129,653,155 (GRCm39)		probably benign	Het
Stxbp5	T	A	10: 9,713,836 (GRCm39)	R234S	probably damaging	Het
Tifab	A	G	13: 56,324,101 (GRCm39)	V114A	probably benign	Het
Tiprl	A	G	1: 165,055,975 (GRCm39)	M49T	probably benign	Het
Tlr12	A	G	4: 128,511,545 (GRCm39)	L235P	possibly damaging	Het
Trim43b	A	T	9: 88,967,411 (GRCm39)	C407*	probably null	Het
Txndc17	C	A	11: 72,098,533 (GRCm39)	F28L	probably damaging	Het
Vmn2r27	T	C	6: 124,177,649 (GRCm39)	R452G	probably damaging	Het
Vmn2r3	T	A	3: 64,182,538 (GRCm39)	D387V	probably damaging	Het
Vps13b	T	C	15: 35,875,712 (GRCm39)	I2699T	probably damaging	Het
Zfp944	G	T	17: 22,558,697 (GRCm39)	Y183*	probably null	Het

Other mutations in Or13c25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00159:Or13c25	APN	4	52,911,618 (GRCm39)	missense	possibly damaging	0.95
IGL02224:Or13c25	APN	4	52,911,392 (GRCm39)	missense	probably damaging	0.97
IGL03293:Or13c25	APN	4	52,910,835 (GRCm39)	makesense	probably null
K3955:Or13c25	UTSW	4	52,911,081 (GRCm39)	missense	probably damaging	1.00
R0195:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign	0.00
R0197:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign	0.00
R0445:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign	0.00
R1517:Or13c25	UTSW	4	52,911,502 (GRCm39)	nonsense	probably null
R1540:Or13c25	UTSW	4	52,910,996 (GRCm39)	missense	probably benign	0.00
R1551:Or13c25	UTSW	4	52,911,397 (GRCm39)	nonsense	probably null
R1612:Or13c25	UTSW	4	52,911,501 (GRCm39)	missense	probably benign
R1920:Or13c25	UTSW	4	52,910,849 (GRCm39)	missense	probably benign
R2181:Or13c25	UTSW	4	52,911,524 (GRCm39)	missense	probably damaging	1.00
R5410:Or13c25	UTSW	4	52,910,991 (GRCm39)	missense	probably benign	0.01
R6331:Or13c25	UTSW	4	52,911,399 (GRCm39)	missense	probably damaging	1.00
R6336:Or13c25	UTSW	4	52,911,459 (GRCm39)	missense	probably damaging	1.00
R7085:Or13c25	UTSW	4	52,910,961 (GRCm39)	missense	probably benign	0.02
R7541:Or13c25	UTSW	4	52,911,376 (GRCm39)	missense	probably benign	0.04
R7727:Or13c25	UTSW	4	52,911,368 (GRCm39)	missense	possibly damaging	0.89
R7891:Or13c25	UTSW	4	52,911,663 (GRCm39)	missense	probably benign	0.01
R8782:Or13c25	UTSW	4	52,911,693 (GRCm39)	missense	probably benign	0.16
R9321:Or13c25	UTSW	4	52,911,314 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCTTTGCGTACATGGAGAAGATGG -3'
(R):5'- TTCTAGGCACGATGGCACTTGAC -3'

Sequencing Primer
(F):5'- ATAATTACCACTGTCAGGTGGGC -3'
(R):5'- ATGGCACTTGACCGCTTTATG -3'

Posted On

2014-04-13