Mutagenetix > Incidental Mutations

Incidental Mutation 'R1549:Olfr1369-ps1'

169798

Institutional Source

Beutler Lab

Gene Symbol

Olfr1369-ps1

Ensembl Gene

ENSMUSG00000060404

Gene Name

olfactory receptor 1369, pseudogene 1

Synonyms

GA_x6K02T2QHY8-12126170-12125935, MOR256-31

MMRRC Submission

039588-MU

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.315) question?

Stock #

R1549 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

21111483-21119095 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 21116118 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 142 (V142A)

Ref Sequence

ENSEMBL: ENSMUSP00000151041 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079050] [ENSMUST00000213326] [ENSMUST00000213922] [ENSMUST00000215207] [ENSMUST00000215941]

AlphaFold

A0A140T8K7

Predicted Effect

probably benign
Transcript: ENSMUST00000079050
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000078059
Gene: ENSMUSG00000060404
AA Change: V142A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	6.2e-47	PFAM
Pfam:7tm_1	41	290	1.1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213326
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000213922
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000215207
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000215941
AA Change: V142A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.8%
20x: 90.8%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	A	T	3: 124,416,792	I157K	probably benign	Het
Ank3	A	T	10: 70,001,982	N727Y	probably benign	Het
Atg16l1	A	G	1: 87,774,188	T251A	probably benign	Het
Bcl11b	T	A	12: 107,917,163	I226F	probably damaging	Het
Birc6	A	G	17: 74,662,742	H4260R	probably damaging	Het
Camta1	A	G	4: 151,586,463	I85T	probably damaging	Het
Cblb	A	G	16: 52,033,010		probably benign	Het
Ccnd1	T	G	7: 144,937,336	I178L	probably benign	Het
Clcn4	A	G	7: 7,291,682	V329A	probably damaging	Het
Col17a1	C	T	19: 47,648,910		probably benign	Het
Col7a1	A	G	9: 108,955,966	T254A	unknown	Het
Ctif	C	A	18: 75,565,025	R188L	probably damaging	Het
Cyp2c69	A	T	19: 39,842,986	L461Q	probably benign	Het
Ddc	A	G	11: 11,846,656		probably null	Het
Dpp10	T	A	1: 123,341,380		probably null	Het
Eef2	CCC	CCCC	10: 81,178,768		probably null	Het
Eif2b4	T	C	5: 31,192,921	E19G	possibly damaging	Het
Fmnl2	A	G	2: 53,105,537	E424G	probably damaging	Het
Galnt14	A	G	17: 73,525,313	L269P	possibly damaging	Het
Gm14409	T	C	2: 177,265,585	E40G	probably damaging	Het
Gm2381	T	A	7: 42,822,401	H18L	probably benign	Het
Gpbp1	G	T	13: 111,436,579	D326E	probably benign	Het
Gpr162	C	T	6: 124,860,088	R331H	probably damaging	Het
Iigp1	G	A	18: 60,389,876	G22D	probably benign	Het
Kcns3	T	C	12: 11,092,083	H205R	probably damaging	Het
Klk1b26	C	T	7: 44,016,402		probably benign	Het
Lime1	A	G	2: 181,383,376	Y242C	probably benign	Het
Manba	A	G	3: 135,544,806	D398G	probably damaging	Het
Mapk3	A	T	7: 126,763,512	K219*	probably null	Het
Mdfic	G	T	6: 15,799,845	G324C	probably damaging	Het
Mdga1	G	A	17: 29,837,998	H837Y	probably damaging	Het
Nck1	T	A	9: 100,497,872	M109L	probably benign	Het
Olfr412	A	G	11: 74,365,250	I194V	probably benign	Het
Pabpc1l	G	T	2: 164,037,171	V313F	possibly damaging	Het
Phf3	C	T	1: 30,804,842	V1679I	probably benign	Het
Pou4f1	T	C	14: 104,467,640	I32V	probably benign	Het
Pspc1	T	G	14: 56,748,941	H351P	probably damaging	Het
Ptk7	T	C	17: 46,572,652	E829G	probably damaging	Het
Rab23	A	G	1: 33,738,297	Y164C	possibly damaging	Het
Slc36a3	A	G	11: 55,142,770	W141R	probably damaging	Het
Slc7a14	T	C	3: 31,224,118	E446G	possibly damaging	Het
Stim2	G	A	5: 54,105,325	R303Q	probably damaging	Het
Tbcd	A	G	11: 121,560,753	I550V	probably benign	Het
Tmed3	A	C	9: 89,699,945	L155R	probably damaging	Het
Trav16d-dv11	A	G	14: 53,047,342		probably benign	Het
Ubap2	C	T	4: 41,199,872	A752T	probably benign	Het
Usp16	G	T	16: 87,464,834	V113F	probably damaging	Het
Vmn2r12	T	G	5: 109,092,830	Y139S	probably benign	Het
Vmn2r5	G	T	3: 64,504,000	D382E	probably damaging	Het
Zfp217	A	C	2: 170,114,470	N869K	probably benign	Het
Zswim2	G	T	2: 83,923,748	D189E	probably benign	Het

Other mutations in Olfr1369-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01689:Olfr1369-ps1	APN	13	21116073	missense	probably damaging	0.97
R0631:Olfr1369-ps1	UTSW	13	21115908	missense	probably damaging	1.00
R0960:Olfr1369-ps1	UTSW	13	21116265	missense	possibly damaging	0.94
R1499:Olfr1369-ps1	UTSW	13	21116133	missense	probably benign	0.32
R1698:Olfr1369-ps1	UTSW	13	21116565	missense	probably benign	0.11
R1711:Olfr1369-ps1	UTSW	13	21116306	missense	probably benign	0.01
R2404:Olfr1369-ps1	UTSW	13	21115842	missense	probably damaging	1.00
R2471:Olfr1369-ps1	UTSW	13	21116429	missense	probably damaging	1.00
R3844:Olfr1369-ps1	UTSW	13	21116063	missense	possibly damaging	0.91
R3977:Olfr1369-ps1	UTSW	13	21115861	missense	probably benign	0.03
R3979:Olfr1369-ps1	UTSW	13	21115861	missense	probably benign	0.03
R4804:Olfr1369-ps1	UTSW	13	21116005	nonsense	probably null
R4914:Olfr1369-ps1	UTSW	13	21116397	missense	probably benign	0.12
R5210:Olfr1369-ps1	UTSW	13	21116052	missense	probably damaging	0.99
R5359:Olfr1369-ps1	UTSW	13	21116267	missense	probably damaging	1.00
R5700:Olfr1369-ps1	UTSW	13	21116001	missense	probably damaging	1.00
R6218:Olfr1369-ps1	UTSW	13	21116231	missense	probably damaging	1.00
R6767:Olfr1369-ps1	UTSW	13	21116057	missense	probably benign	0.02
R7396:Olfr1369-ps1	UTSW	13	21116307	missense	probably benign	0.02
R7476:Olfr1369-ps1	UTSW	13	21116021	missense	probably benign	0.04
R7612:Olfr1369-ps1	UTSW	13	21116047	missense	probably damaging	0.99
R8257:Olfr1369-ps1	UTSW	13	21116373	missense	probably benign	0.11
R9388:Olfr1369-ps1	UTSW	13	21116604	missense	probably damaging	0.96
V8831:Olfr1369-ps1	UTSW	13	21116003	missense	possibly damaging	0.93
Z1176:Olfr1369-ps1	UTSW	13	21116601	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGACCCGAAAAGACCATTAGCTCTG -3'
(R):5'- CTGCCTTTGACTTCATGTTGAGCAC -3'

Sequencing Primer
(F):5'- CATTAGCTCTGTGGGCTGTATTG -3'
(R):5'- ACTTCATGTTGAGCACAGTTTTG -3'

Posted On

2014-04-13