Mutagenetix > Incidental Mutations

Incidental Mutation 'R1552:Dclk3'

170022

Institutional Source

Beutler Lab

Gene Symbol

Dclk3

Ensembl Gene

ENSMUSG00000032500

Gene Name

doublecortin-like kinase 3

Synonyms

Click-I, -II related, Dcamkl3

MMRRC Submission

039591-MU

Accession Numbers

Genbank: NM_172928; MGI: 3039580

Is this an essential gene?

Possibly non essential (E-score: 0.304) question?

Stock #

R1552 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

111439081-111489118 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 111488579 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 761 (T761K)

Ref Sequence

ENSEMBL: ENSMUSP00000107510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111879]

AlphaFold

Q8BWQ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000111879
AA Change: T761K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500
AA Change: T761K

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
Pfam:DCX	115	177	2.9e-17	PFAM
low complexity region	200	218	N/A	INTRINSIC
low complexity region	267	277	N/A	INTRINSIC
low complexity region	479	496	N/A	INTRINSIC
S_TKc	514	771	1.63e-110	SMART

Meta Mutation Damage Score

0.3703

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.1%
20x: 91.7%

Validation Efficiency

97% (71/73)

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810046K07Rik	T	A	9: 51,291,570	S95C	probably damaging	Het
Abcc5	T	A	16: 20,398,867	I365F	probably damaging	Het
Abhd15	T	C	11: 77,515,407	L70P	probably damaging	Het
Adam18	A	C	8: 24,646,361	H381Q	probably benign	Het
Ankfy1	T	C	11: 72,754,495		probably null	Het
Arcn1	C	T	9: 44,758,994	A112T	probably damaging	Het
Calhm1	C	T	19: 47,141,201	R294H	probably benign	Het
Ccdc121	A	G	1: 181,510,991	L132P	probably damaging	Het
Cdk5rap1	T	C	2: 154,370,695	E81G	probably benign	Het
Cep170	T	C	1: 176,782,494		probably benign	Het
Cep350	C	T	1: 155,910,738	R1454Q	possibly damaging	Het
Chrna3	C	A	9: 55,015,908	E205D	probably benign	Het
Chst5	T	C	8: 111,890,280	D236G	probably damaging	Het
Coro1a	T	C	7: 126,699,952	N367D	probably benign	Het
Cyp3a16	T	C	5: 145,436,536	I474V	probably benign	Het
Cyth3	G	A	5: 143,697,750	V87I	probably benign	Het
Dvl2	T	A	11: 70,006,372	M300K	possibly damaging	Het
Eefsec	C	T	6: 88,376,200		probably benign	Het
Exog	T	C	9: 119,445,110	S54P	unknown	Het
Fasn	A	G	11: 120,818,558	S519P	probably damaging	Het
Gas8	G	A	8: 123,520,646	A16T	probably benign	Het
Got2	A	G	8: 95,869,494	S333P	probably benign	Het
Hadhb	T	A	5: 30,176,933	L287Q	probably null	Het
Ift57	A	G	16: 49,759,353	T211A	probably benign	Het
Il1rap	A	G	16: 26,722,434	E475G	possibly damaging	Het
Ilkap	A	T	1: 91,384,594	D11E	probably damaging	Het
Impact	T	C	18: 12,984,280	S137P	probably benign	Het
Jarid2	T	C	13: 44,911,199	V920A	probably damaging	Het
Kcnk18	A	T	19: 59,235,458	H345L	probably damaging	Het
Kdm4d	A	T	9: 14,464,029	Y178N	probably damaging	Het
Klk1b1	A	G	7: 43,969,343	Y48C	probably damaging	Het
Klra5	G	T	6: 129,909,885	T60K	probably damaging	Het
Kng2	A	T	16: 22,987,520	L643H	probably damaging	Het
Lamb3	A	G	1: 193,330,759		probably null	Het
Lingo2	A	G	4: 35,708,315	V555A	probably damaging	Het
Mbd5	T	C	2: 49,272,934	S251P	probably damaging	Het
Mc4r	T	C	18: 66,859,695	S116G	probably benign	Het
Mipol1	T	C	12: 57,306,088	V71A	possibly damaging	Het
Myo15b	A	C	11: 115,866,635	S1104R	probably benign	Het
Nek1	T	A	8: 61,006,737	D26E	probably damaging	Het
Neu1	C	T	17: 34,932,113		probably benign	Het
Npffr2	T	C	5: 89,583,116	S302P	possibly damaging	Het
Olfr912	T	C	9: 38,581,379	M34T	probably benign	Het
Palmd	T	A	3: 116,948,040		probably benign	Het
Pcdh8	A	T	14: 79,770,607	V172E	probably benign	Het
Pnpla6	C	A	8: 3,522,403	Q291K	probably damaging	Het
Prkch	T	C	12: 73,702,546	F357L	probably benign	Het
Ptprj	A	T	2: 90,471,153	Y212N	probably damaging	Het
Reln	G	T	5: 21,960,378	H2061N	probably benign	Het
Rint1	T	A	5: 23,800,658	S113T	probably benign	Het
Rnf38	G	C	4: 44,142,468		probably null	Het
Slc30a4	T	A	2: 122,686,016	I374L	probably benign	Het
Slfn10-ps	T	C	11: 83,029,850		noncoding transcript	Het
Smcp	A	T	3: 92,584,403	C46S	unknown	Het
Smu1	A	C	4: 40,748,570	V240G	probably damaging	Het
Srsf5	A	G	12: 80,949,745		probably benign	Het
Stn1	T	C	19: 47,536,373		probably null	Het
Stx18	A	G	5: 38,104,991	E63G	probably damaging	Het
Tas2r130	A	G	6: 131,630,167	Y222H	probably benign	Het
Tescl	G	T	7: 24,333,333	P189Q	probably benign	Het
Tlr1	A	G	5: 64,926,860	S125P	probably damaging	Het
Ugt2a2	G	T	5: 87,462,021	D566E	possibly damaging	Het
Uhrf1bp1	T	C	17: 27,890,071	F1088S	possibly damaging	Het
Unc13b	A	T	4: 43,237,144	T3405S	probably damaging	Het
Upf1	G	A	8: 70,333,059	Q1046*	probably null	Het
Wwox	T	A	8: 114,445,350	Y61*	probably null	Het
Zfhx4	C	T	3: 5,403,110	T2776M	probably damaging	Het
Zranb3	G	A	1: 127,960,751		probably benign	Het

Other mutations in Dclk3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Dclk3	APN	9	111467843	nonsense	probably null
IGL02125:Dclk3	APN	9	111469107	missense	probably damaging	1.00
IGL02547:Dclk3	APN	9	111469023	missense	probably damaging	1.00
IGL03393:Dclk3	APN	9	111488673	utr 3 prime	probably benign
G1citation:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
IGL02984:Dclk3	UTSW	9	111488575	missense	probably damaging	1.00
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0432:Dclk3	UTSW	9	111484935	missense	probably damaging	1.00
R0440:Dclk3	UTSW	9	111469163	missense	probably damaging	1.00
R0530:Dclk3	UTSW	9	111482721	missense	probably damaging	1.00
R1024:Dclk3	UTSW	9	111469070	missense	possibly damaging	0.95
R1443:Dclk3	UTSW	9	111469020	missense	probably benign	0.01
R1474:Dclk3	UTSW	9	111469236	missense	probably benign	0.43
R1479:Dclk3	UTSW	9	111468546	missense	probably benign
R1482:Dclk3	UTSW	9	111467820	missense	possibly damaging	0.90
R1543:Dclk3	UTSW	9	111468054	missense	probably benign	0.04
R1559:Dclk3	UTSW	9	111469208	missense	probably damaging	1.00
R2011:Dclk3	UTSW	9	111468354	missense	probably benign	0.00
R2369:Dclk3	UTSW	9	111488542	missense	probably benign	0.16
R4111:Dclk3	UTSW	9	111469080	missense	probably damaging	0.99
R4510:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4511:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4592:Dclk3	UTSW	9	111467895	missense	probably damaging	1.00
R4604:Dclk3	UTSW	9	111469185	missense	probably damaging	1.00
R4857:Dclk3	UTSW	9	111468648	missense	probably benign
R4932:Dclk3	UTSW	9	111468042	missense	possibly damaging	0.56
R5045:Dclk3	UTSW	9	111467788	missense	probably damaging	0.99
R5233:Dclk3	UTSW	9	111468681	missense	probably benign
R5338:Dclk3	UTSW	9	111469059	missense	possibly damaging	0.95
R5463:Dclk3	UTSW	9	111469260	missense	probably benign	0.26
R6822:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
R6995:Dclk3	UTSW	9	111467700	missense	possibly damaging	0.88
R7187:Dclk3	UTSW	9	111484996	missense	probably damaging	1.00
R7532:Dclk3	UTSW	9	111467528	missense	probably benign	0.03
R7534:Dclk3	UTSW	9	111468218	missense	probably benign
R7734:Dclk3	UTSW	9	111469095	missense	probably damaging	1.00
R8326:Dclk3	UTSW	9	111467534	missense	probably damaging	0.96
R8372:Dclk3	UTSW	9	111485013	missense	probably damaging	0.98
R8388:Dclk3	UTSW	9	111482745	missense	probably damaging	1.00
R8423:Dclk3	UTSW	9	111468719	missense	possibly damaging	0.93
R8493:Dclk3	UTSW	9	111468147	missense	probably benign	0.06
R9013:Dclk3	UTSW	9	111468498	missense	probably benign	0.31
R9114:Dclk3	UTSW	9	111488615	missense	probably benign	0.06
R9338:Dclk3	UTSW	9	111439305	missense	unknown
R9412:Dclk3	UTSW	9	111482751	critical splice donor site	probably null
X0020:Dclk3	UTSW	9	111485075	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCAGGTTGCCCTGGACAAGGAAAG -3'
(R):5'- TCTTGCACAGCCTTGCTTGTAGAC -3'

Sequencing Primer
(F):5'- CAAGGAAAGCTTATTAGTCCTTCCC -3'
(R):5'- ACAGCCTTGCTTGTAGACTAGAC -3'

Posted On

2014-04-13