Mutagenetix > Incidental Mutations

Incidental Mutation 'R1559:Dclk3'

170505

Institutional Source

Beutler Lab

Gene Symbol

Dclk3

Ensembl Gene

ENSMUSG00000032500

Gene Name

doublecortin-like kinase 3

Synonyms

Click-I, -II related, Dcamkl3

MMRRC Submission

039598-MU

Accession Numbers

Genbank: NM_172928; MGI: 3039580

Is this an essential gene?

Possibly non essential (E-score: 0.277) question?

Stock #

R1559 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

111439081-111489118 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 111469208 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 607 (F607L)

Ref Sequence

ENSEMBL: ENSMUSP00000107510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111879]

AlphaFold

Q8BWQ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000111879
AA Change: F607L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500
AA Change: F607L

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
Pfam:DCX	115	177	2.9e-17	PFAM
low complexity region	200	218	N/A	INTRINSIC
low complexity region	267	277	N/A	INTRINSIC
low complexity region	479	496	N/A	INTRINSIC
S_TKc	514	771	1.63e-110	SMART

Meta Mutation Damage Score

0.4258

Coding Region Coverage

1x: 98.8%
3x: 97.7%
10x: 94.0%
20x: 84.8%

Validation Efficiency

95% (73/77)

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,399,180	W3585R	probably null	Het
Babam1	G	C	8: 71,397,780	E18Q	probably damaging	Het
Bcan	G	T	3: 87,994,212	S394R	probably damaging	Het
Birc6	T	A	17: 74,692,237	F4653L	probably damaging	Het
Camsap2	A	T	1: 136,282,094	H559Q	probably benign	Het
Cars2	G	T	8: 11,530,430		probably null	Het
Ccdc93	T	A	1: 121,461,983		probably benign	Het
Ccna2	C	A	3: 36,570,730		probably benign	Het
Cdc6	T	A	11: 98,912,211	L326I	probably damaging	Het
Cdh23	A	T	10: 60,419,699		probably benign	Het
Cenpe	T	A	3: 135,270,900	S2423T	probably benign	Het
Cftr	A	G	6: 18,225,937	M295V	probably benign	Het
Cxcl2	A	G	5: 90,904,012	H23R	probably benign	Het
Cyp4a12b	A	G	4: 115,433,984	T370A	probably damaging	Het
Daam2	A	G	17: 49,496,120		probably benign	Het
Des	T	G	1: 75,360,586	S57A	probably benign	Het
Drd1	A	T	13: 54,052,945	S410T	probably damaging	Het
Ecm1	G	A	3: 95,735,963	R342C	probably damaging	Het
Fanci	T	C	7: 79,433,193	L639P	probably damaging	Het
Fancm	A	G	12: 65,093,689	E395G	probably benign	Het
Gm996	G	T	2: 25,577,031	S956*	probably null	Het
Grik3	A	G	4: 125,707,997	D889G	probably benign	Het
Heyl	A	G	4: 123,241,399	S62G	probably damaging	Het
Hmox1	C	T	8: 75,099,949	P267L	probably damaging	Het
Ifi213	A	C	1: 173,567,218	S584A	probably benign	Het
Il12rb2	G	T	6: 67,356,592	F234L	probably benign	Het
Il4i1	T	A	7: 44,839,387	S233T	probably damaging	Het
Itga4	A	G	2: 79,315,688	S745G	probably benign	Het
Kalrn	T	A	16: 34,010,548	I734F	possibly damaging	Het
Klk1b16	T	C	7: 44,141,001	I200T	probably benign	Het
Lrrc4c	T	A	2: 97,630,772	M581K	probably benign	Het
M6pr	A	T	6: 122,315,074	I122L	probably benign	Het
Magi3	T	C	3: 104,046,853		probably benign	Het
Mybpc2	T	G	7: 44,513,687	T480P	probably benign	Het
Olfr301	T	C	7: 86,412,367	S43P	probably benign	Het
Olfr346	A	G	2: 36,688,758	Y252C	probably damaging	Het
Olfr613	A	T	7: 103,552,165	I127F	possibly damaging	Het
Olfr937	T	C	9: 39,060,141	N175S	probably benign	Het
Pcx	T	A	19: 4,619,086	I704N	probably damaging	Het
Pde3a	G	T	6: 141,459,098	A350S	probably damaging	Het
Ppox	A	T	1: 171,280,006		probably benign	Het
Ppp1r32	T	C	19: 10,481,406	T87A	probably benign	Het
Prb1	T	A	6: 132,208,544	Y42F	unknown	Het
Rsph4a	T	A	10: 33,909,731	V546E	probably damaging	Het
Sf3b1	C	G	1: 55,019,395	E12Q	possibly damaging	Het
Sh3tc1	A	G	5: 35,703,349		probably null	Het
Slc22a2	T	C	17: 12,584,411	F44S	probably damaging	Het
Slco6c1	T	A	1: 97,098,498	D334V	probably damaging	Het
Smim19	T	C	8: 22,463,336	D105G	probably damaging	Het
Smpdl3a	A	G	10: 57,807,492	T233A	probably damaging	Het
St8sia5	G	A	18: 77,211,764		probably null	Het
Stk32a	C	A	18: 43,243,084	Q73K	probably benign	Het
Tmem129	G	T	5: 33,657,756		probably null	Het
Traf3ip3	A	T	1: 193,178,291	L441Q	probably damaging	Het
Ttn	C	T	2: 76,900,961		probably benign	Het
Unc45b	T	C	11: 82,917,846	S253P	possibly damaging	Het
Vars2	A	G	17: 35,666,258		probably benign	Het
Vmn2r51	C	T	7: 10,102,445	M136I	possibly damaging	Het
Vmn2r51	A	G	7: 10,102,446	M136T	possibly damaging	Het
Zfp595	T	C	13: 67,317,063	I379V	possibly damaging	Het

Other mutations in Dclk3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Dclk3	APN	9	111467843	nonsense	probably null
IGL02125:Dclk3	APN	9	111469107	missense	probably damaging	1.00
IGL02547:Dclk3	APN	9	111469023	missense	probably damaging	1.00
IGL03393:Dclk3	APN	9	111488673	utr 3 prime	probably benign
G1citation:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
IGL02984:Dclk3	UTSW	9	111488575	missense	probably damaging	1.00
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0432:Dclk3	UTSW	9	111484935	missense	probably damaging	1.00
R0440:Dclk3	UTSW	9	111469163	missense	probably damaging	1.00
R0530:Dclk3	UTSW	9	111482721	missense	probably damaging	1.00
R1024:Dclk3	UTSW	9	111469070	missense	possibly damaging	0.95
R1443:Dclk3	UTSW	9	111469020	missense	probably benign	0.01
R1474:Dclk3	UTSW	9	111469236	missense	probably benign	0.43
R1479:Dclk3	UTSW	9	111468546	missense	probably benign
R1482:Dclk3	UTSW	9	111467820	missense	possibly damaging	0.90
R1543:Dclk3	UTSW	9	111468054	missense	probably benign	0.04
R1552:Dclk3	UTSW	9	111488579	missense	probably damaging	1.00
R2011:Dclk3	UTSW	9	111468354	missense	probably benign	0.00
R2369:Dclk3	UTSW	9	111488542	missense	probably benign	0.16
R4111:Dclk3	UTSW	9	111469080	missense	probably damaging	0.99
R4510:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4511:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4592:Dclk3	UTSW	9	111467895	missense	probably damaging	1.00
R4604:Dclk3	UTSW	9	111469185	missense	probably damaging	1.00
R4857:Dclk3	UTSW	9	111468648	missense	probably benign
R4932:Dclk3	UTSW	9	111468042	missense	possibly damaging	0.56
R5045:Dclk3	UTSW	9	111467788	missense	probably damaging	0.99
R5233:Dclk3	UTSW	9	111468681	missense	probably benign
R5338:Dclk3	UTSW	9	111469059	missense	possibly damaging	0.95
R5463:Dclk3	UTSW	9	111469260	missense	probably benign	0.26
R6822:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
R6995:Dclk3	UTSW	9	111467700	missense	possibly damaging	0.88
R7187:Dclk3	UTSW	9	111484996	missense	probably damaging	1.00
R7532:Dclk3	UTSW	9	111467528	missense	probably benign	0.03
R7534:Dclk3	UTSW	9	111468218	missense	probably benign
R7734:Dclk3	UTSW	9	111469095	missense	probably damaging	1.00
R8326:Dclk3	UTSW	9	111467534	missense	probably damaging	0.96
R8372:Dclk3	UTSW	9	111485013	missense	probably damaging	0.98
R8388:Dclk3	UTSW	9	111482745	missense	probably damaging	1.00
R8423:Dclk3	UTSW	9	111468719	missense	possibly damaging	0.93
R8493:Dclk3	UTSW	9	111468147	missense	probably benign	0.06
R9013:Dclk3	UTSW	9	111468498	missense	probably benign	0.31
R9114:Dclk3	UTSW	9	111488615	missense	probably benign	0.06
R9338:Dclk3	UTSW	9	111439305	missense	unknown
R9412:Dclk3	UTSW	9	111482751	critical splice donor site	probably null
X0020:Dclk3	UTSW	9	111485075	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTACGCCATGAAGATGATTGACAAGTCC -3'
(R):5'- CACTGAGAAGCAGTTGATATTCATCCCA -3'

Sequencing Primer
(F):5'- TGTGAAACTGCACGAGGT -3'
(R):5'- AGCAGTTGATATTCATCCCATTCTAC -3'

Posted On

2014-04-13