Mutagenetix > Incidental Mutations

Incidental Mutation 'R1561:Trappc8'

170642

Institutional Source

Beutler Lab

Gene Symbol

Trappc8

Ensembl Gene

ENSMUSG00000033382

Gene Name

trafficking protein particle complex 8

Synonyms

D030074E01Rik, Trs85, 5033403J15Rik

MMRRC Submission

039600-MU

Accession Numbers

Genbank: NM_029491; MGI: 2443008

Is this an essential gene?

Probably essential (E-score: 0.948) question?

Stock #

R1561 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20817223-20896093 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 20841623 bp

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 883 (R883*)

Ref Sequence

ENSEMBL: ENSMUSP00000153183 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025177] [ENSMUST00000097658] [ENSMUST00000225661]

Predicted Effect

probably null
Transcript: ENSMUST00000025177
AA Change: R884*

SMART Domains

Protein: ENSMUSP00000025177
Gene: ENSMUSG00000033382
AA Change: R884*

Domain	Start	End	E-Value	Type
Pfam:TRAPPC-Trs85	157	604	1e-167	PFAM
low complexity region	769	777	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000097658

SMART Domains

Protein: ENSMUSP00000095262
Gene: ENSMUSG00000033382

Domain	Start	End	E-Value	Type
Pfam:TRAPPC-Trs85	152	605	9.3e-135	PFAM
low complexity region	769	777	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000157924

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000223584

Predicted Effect

probably null
Transcript: ENSMUST00000225661
AA Change: R883*

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 89.9%

Validation Efficiency

Allele List at MGI

All alleles(11) : Gene trapped(11)

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700061G19Rik	A	G	17: 56,877,431	N69D	probably benign	Het
Alms1	T	A	6: 85,629,052	Y2561*	probably null	Het
Ap1g2	T	C	14: 55,104,887	E171G	probably damaging	Het
Atg7	T	C	6: 114,701,172	V341A	possibly damaging	Het
Bace1	G	T	9: 45,839,194	R56L	probably benign	Het
Chfr	A	T	5: 110,158,808	D472V	probably benign	Het
Ckap2l	G	A	2: 129,270,725	T621I	probably benign	Het
Cmip	A	G	8: 117,453,850	T554A	probably benign	Het
Crocc	C	T	4: 141,030,268	E905K	probably damaging	Het
D6Ertd527e	C	G	6: 87,111,524	T223S	unknown	Het
Dna2	C	T	10: 62,949,187	R28W	probably benign	Het
Ecm1	G	A	3: 95,735,963	R342C	probably damaging	Het
F5	C	A	1: 164,186,903	S581*	probably null	Het
Fam227a	T	A	15: 79,636,762	Y291F	possibly damaging	Het
Gm609	A	G	16: 45,442,512	V88A	possibly damaging	Het
Gpr151	A	T	18: 42,579,156	S152R	probably benign	Het
Gpr158	A	T	2: 21,815,694		probably null	Het
Kcna5	T	C	6: 126,534,583	Y194C	probably damaging	Het
Khsrp	A	G	17: 57,025,639	S214P	probably benign	Het
Mrgprb1	C	G	7: 48,447,125		probably null	Het
Mrnip	C	T	11: 50,176,849	T30I	probably damaging	Het
Mtus2	A	C	5: 148,076,552	K52Q	probably benign	Het
Naca	G	T	10: 128,040,398		probably benign	Het
Obscn	T	G	11: 59,036,073	T5539P	probably damaging	Het
Olfr1154	C	T	2: 87,903,161	V172I	probably benign	Het
Olfr1341	T	A	4: 118,709,554	I49N	probably damaging	Het
Ovca2	A	G	11: 75,177,979	L198P	probably damaging	Het
Pdzrn4	T	C	15: 92,677,637	V308A	possibly damaging	Het
Pkd1l3	A	G	8: 109,614,813	I99M	unknown	Het
Polr3b	T	A	10: 84,634,912	M139K	probably damaging	Het
Prkag2	T	C	5: 24,871,595	Y191C	probably damaging	Het
Prss47	A	T	13: 65,046,248	C278S	probably damaging	Het
Ptprm	T	C	17: 66,940,541	T600A	probably damaging	Het
Ruvbl1	T	C	6: 88,479,154	V70A	probably damaging	Het
Sf3a1	T	C	11: 4,179,217	V726A	probably benign	Het
Sf3b1	C	G	1: 55,019,395	E12Q	possibly damaging	Het
Slc26a3	A	G	12: 31,466,452	N603S	probably benign	Het
Slc2a1	A	G	4: 119,136,409	E481G	possibly damaging	Het
Slc35a5	A	C	16: 45,151,521	S127A	possibly damaging	Het
Spen	C	A	4: 141,472,383	G2978*	probably null	Het
Srrt	T	A	5: 137,300,019	E297V	probably benign	Het
Srsf4	T	A	4: 131,897,695	D134E	probably damaging	Het
Tdrd6	G	A	17: 43,625,624	S1511L	probably damaging	Het
Tmem65	T	A	15: 58,822,858	I91F	probably benign	Het
Top2b	A	G	14: 16,398,993	K538E	possibly damaging	Het
Ttc28	G	A	5: 111,225,677	S962N	probably damaging	Het
Vav3	T	C	3: 109,494,838		probably null	Het
Vmn1r42	C	T	6: 89,845,381	G69S	probably damaging	Het
Zan	A	T	5: 137,380,838	Y5333*	probably null	Het
Zfp994	A	C	17: 22,201,225	F248V	probably damaging	Het

Other mutations in Trappc8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01077:Trappc8	APN	18	20836978	missense	probably benign	0.20
IGL01367:Trappc8	APN	18	20866119	missense	probably benign	0.01
IGL01537:Trappc8	APN	18	20835004	missense	probably benign
IGL01563:Trappc8	APN	18	20837046	missense	probably benign	0.00
IGL01982:Trappc8	APN	18	20874712	splice site	probably benign
IGL02709:Trappc8	APN	18	20837178	missense	possibly damaging	0.94
IGL03126:Trappc8	APN	18	20863595	missense	probably damaging	1.00
IGL03290:Trappc8	APN	18	20820935	missense	probably damaging	1.00
IGL03348:Trappc8	APN	18	20852781	missense	probably damaging	1.00
hoppa	UTSW	18	20836900	missense	probably benign	0.05
Lagomorpha	UTSW	18	20818190	missense	probably benign	0.11
rabbit	UTSW	18	20874680	missense	probably damaging	1.00
E7848:Trappc8	UTSW	18	20850918	missense	probably damaging	0.99
R0483:Trappc8	UTSW	18	20845601	missense	possibly damaging	0.60
R0492:Trappc8	UTSW	18	20866186	missense	probably benign	0.07
R0506:Trappc8	UTSW	18	20844188	missense	possibly damaging	0.49
R0610:Trappc8	UTSW	18	20837188	missense	probably damaging	1.00
R0892:Trappc8	UTSW	18	20831608	critical splice donor site	probably null
R1589:Trappc8	UTSW	18	20863551	missense	probably damaging	1.00
R1700:Trappc8	UTSW	18	20832998	missense	probably damaging	1.00
R1785:Trappc8	UTSW	18	20834940	splice site	probably null
R1786:Trappc8	UTSW	18	20834940	splice site	probably null
R1989:Trappc8	UTSW	18	20845651	missense	probably benign	0.04
R2181:Trappc8	UTSW	18	20819222	critical splice donor site	probably null
R2294:Trappc8	UTSW	18	20866154	nonsense	probably null
R4551:Trappc8	UTSW	18	20874672	missense	probably benign	0.10
R4594:Trappc8	UTSW	18	20836948	missense	probably benign
R4631:Trappc8	UTSW	18	20867808	missense	probably benign	0.22
R4734:Trappc8	UTSW	18	20841572	nonsense	probably null
R4834:Trappc8	UTSW	18	20825065	missense	probably damaging	0.99
R5114:Trappc8	UTSW	18	20844180	missense	probably benign	0.04
R5262:Trappc8	UTSW	18	20818190	missense	probably benign	0.11
R5384:Trappc8	UTSW	18	20833062	splice site	probably null
R5476:Trappc8	UTSW	18	20865108	missense	probably damaging	1.00
R5503:Trappc8	UTSW	18	20836900	missense	probably benign	0.05
R5577:Trappc8	UTSW	18	20836779	nonsense	probably null
R5809:Trappc8	UTSW	18	20818082	missense	probably benign	0.08
R5825:Trappc8	UTSW	18	20873920	missense	probably damaging	1.00
R5886:Trappc8	UTSW	18	20874680	missense	probably damaging	1.00
R5936:Trappc8	UTSW	18	20874688	missense	probably damaging	1.00
R6024:Trappc8	UTSW	18	20833009	missense	probably damaging	0.98
R6105:Trappc8	UTSW	18	20846447	critical splice donor site	probably null
R6229:Trappc8	UTSW	18	20870745	missense	probably benign	0.00
R6376:Trappc8	UTSW	18	20837075	missense	probably benign	0.07
R6403:Trappc8	UTSW	18	20866071	missense	probably benign
R6459:Trappc8	UTSW	18	20836868	missense	probably benign	0.40
R6673:Trappc8	UTSW	18	20885257	missense	probably benign	0.01
R7041:Trappc8	UTSW	18	20874672	missense	probably benign	0.10
R7276:Trappc8	UTSW	18	20818091	missense	probably damaging	0.99
R7341:Trappc8	UTSW	18	20852647	missense	probably damaging	1.00
R7684:Trappc8	UTSW	18	20863502	missense	probably benign	0.01
R7702:Trappc8	UTSW	18	20825062	missense	probably damaging	0.99
R8210:Trappc8	UTSW	18	20873881	critical splice donor site	probably null
X0065:Trappc8	UTSW	18	20860522	missense	probably benign	0.03
Z1177:Trappc8	UTSW	18	20831663	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- ACATTCTGTGAAACATCATCTGGCTCTG -3'
(R):5'- GCCTTTGGTGAAACCCCACATAGATAC -3'

Sequencing Primer
(F):5'- ACATCATCTGGCTCTGTAATTGTTTG -3'
(R):5'- CAGTGAGACATTGAATAGCATACC -3'

Posted On

2014-04-13