Incidental Mutation 'R1562:Vmn2r4'
| ID |
170658 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Vmn2r4
|
| Ensembl Gene |
ENSMUSG00000092049 |
| Gene Name |
vomeronasal 2, receptor 4 |
| Synonyms |
EG637053 |
| MMRRC Submission |
039601-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.066)
|
| Stock # |
R1562 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
3 |
| Chromosomal Location |
64295982-64322741 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to T
at 64296865 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Asparagine
at position 640
(T640N)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000135228
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000170280]
[ENSMUST00000175724]
|
| AlphaFold |
K7N784 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000170280
AA Change: T551N
PolyPhen 2
Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000127513
Gene: ENSMUSG00000092049
AA Change: T551N
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:ANF_receptor
|
1 |
416 |
2.7e-72 |
PFAM |
|
Pfam:Peripla_BP_6
|
61 |
240 |
1.9e-9 |
PFAM |
|
Pfam:NCD3G
|
458 |
511 |
1.1e-17 |
PFAM |
|
Pfam:7tm_3
|
542 |
779 |
1.8e-75 |
PFAM |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000175724
AA Change: T640N
PolyPhen 2
Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000135228
Gene: ENSMUSG00000092049
AA Change: T640N
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
22 |
N/A |
INTRINSIC |
|
Pfam:ANF_receptor
|
88 |
505 |
2.3e-75 |
PFAM |
|
Pfam:NCD3G
|
547 |
600 |
4.7e-17 |
PFAM |
|
Pfam:7tm_3
|
633 |
867 |
8.2e-47 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.2%
- 10x: 95.8%
- 20x: 91.0%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 64 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abca2 |
A |
G |
2: 25,336,331 (GRCm39) |
I2201V |
probably benign |
Het |
| Adam22 |
T |
C |
5: 8,145,007 (GRCm39) |
N817S |
probably damaging |
Het |
| Alox12 |
C |
A |
11: 70,140,991 (GRCm39) |
R348L |
probably damaging |
Het |
| Asb17 |
A |
T |
3: 153,559,143 (GRCm39) |
T285S |
probably benign |
Het |
| Casp4 |
T |
C |
9: 5,324,733 (GRCm39) |
S182P |
possibly damaging |
Het |
| Cenpe |
T |
C |
3: 134,944,155 (GRCm39) |
M985T |
possibly damaging |
Het |
| Clcn1 |
C |
T |
6: 42,277,169 (GRCm39) |
P420L |
probably benign |
Het |
| Coro2a |
T |
C |
4: 46,548,917 (GRCm39) |
I126V |
probably benign |
Het |
| Cubn |
T |
C |
2: 13,432,778 (GRCm39) |
Y1181C |
probably damaging |
Het |
| Cyp2d22 |
A |
T |
15: 82,258,179 (GRCm39) |
L147Q |
probably damaging |
Het |
| Dna2 |
C |
T |
10: 62,784,966 (GRCm39) |
R28W |
probably benign |
Het |
| Ecm1 |
G |
A |
3: 95,643,275 (GRCm39) |
R342C |
probably damaging |
Het |
| Fat2 |
T |
C |
11: 55,200,800 (GRCm39) |
N758S |
probably damaging |
Het |
| Fbxo43 |
T |
C |
15: 36,163,162 (GRCm39) |
D15G |
probably damaging |
Het |
| Flt3 |
T |
C |
5: 147,281,323 (GRCm39) |
E803G |
probably damaging |
Het |
| Folr1 |
T |
G |
7: 101,507,801 (GRCm39) |
D213A |
probably damaging |
Het |
| Fus |
T |
C |
7: 127,579,094 (GRCm39) |
V359A |
probably damaging |
Het |
| Gabrb3 |
C |
T |
7: 57,415,262 (GRCm39) |
R111* |
probably null |
Het |
| Gm17324 |
T |
C |
9: 78,355,964 (GRCm39) |
|
probably benign |
Het |
| Hormad2 |
A |
T |
11: 4,358,848 (GRCm39) |
|
probably null |
Het |
| Ifi27l2b |
T |
A |
12: 103,422,780 (GRCm39) |
|
probably null |
Het |
| Isg20 |
G |
T |
7: 78,569,891 (GRCm39) |
C176F |
probably benign |
Het |
| Katnip |
C |
A |
7: 125,442,020 (GRCm39) |
S643Y |
probably damaging |
Het |
| Krt15 |
C |
A |
11: 100,024,007 (GRCm39) |
V346L |
probably benign |
Het |
| Liat1 |
A |
T |
11: 75,894,024 (GRCm39) |
I134F |
probably damaging |
Het |
| Med13l |
A |
G |
5: 118,876,584 (GRCm39) |
K920R |
probably damaging |
Het |
| Mlh3 |
A |
T |
12: 85,313,694 (GRCm39) |
F831I |
probably benign |
Het |
| Mtmr9 |
A |
G |
14: 63,771,786 (GRCm39) |
S267P |
probably benign |
Het |
| Mybpc1 |
C |
T |
10: 88,389,193 (GRCm39) |
A406T |
probably damaging |
Het |
| Myh1 |
T |
C |
11: 67,102,196 (GRCm39) |
M829T |
probably benign |
Het |
| Myo10 |
A |
G |
15: 25,780,497 (GRCm39) |
Q209R |
possibly damaging |
Het |
| Nceh1 |
T |
A |
3: 27,293,701 (GRCm39) |
V153D |
probably damaging |
Het |
| Oog3 |
A |
T |
4: 143,889,169 (GRCm39) |
I3N |
probably damaging |
Het |
| Or1j19 |
A |
G |
2: 36,676,696 (GRCm39) |
D53G |
probably damaging |
Het |
| Or2ag1b |
C |
T |
7: 106,288,187 (GRCm39) |
M250I |
probably benign |
Het |
| Or4c103 |
T |
A |
2: 88,513,423 (GRCm39) |
I218F |
probably benign |
Het |
| Or8c20 |
C |
A |
9: 38,260,658 (GRCm39) |
S87* |
probably null |
Het |
| Pcnt |
G |
A |
10: 76,203,164 (GRCm39) |
T2646M |
probably benign |
Het |
| Phf10 |
A |
T |
17: 15,166,512 (GRCm39) |
C453S |
probably damaging |
Het |
| Plcb4 |
T |
A |
2: 135,812,367 (GRCm39) |
|
probably null |
Het |
| Plekhh1 |
A |
G |
12: 79,123,482 (GRCm39) |
H1185R |
probably benign |
Het |
| Prmt3 |
G |
T |
7: 49,476,602 (GRCm39) |
V404L |
probably benign |
Het |
| Ptprb |
T |
A |
10: 116,175,372 (GRCm39) |
D1122E |
probably benign |
Het |
| Rars1 |
A |
G |
11: 35,711,921 (GRCm39) |
|
probably null |
Het |
| Rasa2 |
G |
T |
9: 96,427,803 (GRCm39) |
N687K |
possibly damaging |
Het |
| Rbm11 |
A |
G |
16: 75,393,423 (GRCm39) |
T40A |
probably damaging |
Het |
| Rem2 |
T |
C |
14: 54,713,775 (GRCm39) |
V16A |
probably benign |
Het |
| Rlf |
A |
T |
4: 121,007,588 (GRCm39) |
M574K |
possibly damaging |
Het |
| Rpap3 |
A |
T |
15: 97,592,098 (GRCm39) |
V186D |
possibly damaging |
Het |
| Sertad3 |
G |
A |
7: 27,176,048 (GRCm39) |
E161K |
probably damaging |
Het |
| Sh3gl2 |
T |
C |
4: 85,304,130 (GRCm39) |
S278P |
probably benign |
Het |
| Strn3 |
T |
C |
12: 51,680,401 (GRCm39) |
T400A |
probably benign |
Het |
| Sycp2 |
A |
T |
2: 178,024,178 (GRCm39) |
I402N |
probably damaging |
Het |
| Synj1 |
C |
T |
16: 90,784,290 (GRCm39) |
V283I |
probably benign |
Het |
| Tas2r108 |
A |
G |
6: 40,471,000 (GRCm39) |
|
probably null |
Het |
| Ttpal |
A |
G |
2: 163,457,323 (GRCm39) |
N265S |
probably benign |
Het |
| Unc80 |
G |
A |
1: 66,677,116 (GRCm39) |
G2015D |
probably damaging |
Het |
| Upf1 |
C |
T |
8: 70,796,017 (GRCm39) |
W138* |
probably null |
Het |
| Vmn1r25 |
T |
G |
6: 57,955,786 (GRCm39) |
M168L |
probably benign |
Het |
| Vmn2r18 |
A |
T |
5: 151,510,301 (GRCm39) |
F24Y |
probably benign |
Het |
| Wdr75 |
T |
A |
1: 45,843,030 (GRCm39) |
|
probably null |
Het |
| Zdbf2 |
T |
G |
1: 63,342,747 (GRCm39) |
S375R |
possibly damaging |
Het |
| Zfp648 |
A |
G |
1: 154,080,138 (GRCm39) |
Q99R |
probably benign |
Het |
| Zfp964 |
C |
T |
8: 70,115,654 (GRCm39) |
P85S |
probably benign |
Het |
|
| Other mutations in Vmn2r4 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01310:Vmn2r4
|
APN |
3 |
64,317,200 (GRCm39) |
splice site |
probably null |
|
|
IGL01448:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01452:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01454:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01456:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01463:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01467:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01468:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01470:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01476:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01481:Vmn2r4
|
APN |
3 |
64,313,816 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01534:Vmn2r4
|
APN |
3 |
64,313,844 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01636:Vmn2r4
|
APN |
3 |
64,313,657 (GRCm39) |
missense |
probably benign |
0.21 |
|
IGL01879:Vmn2r4
|
APN |
3 |
64,298,431 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02147:Vmn2r4
|
APN |
3 |
64,305,782 (GRCm39) |
splice site |
probably benign |
|
|
IGL02276:Vmn2r4
|
APN |
3 |
64,313,877 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
IGL02432:Vmn2r4
|
APN |
3 |
64,313,821 (GRCm39) |
missense |
probably benign |
0.38 |
|
IGL02533:Vmn2r4
|
APN |
3 |
64,305,840 (GRCm39) |
nonsense |
probably null |
|
|
IGL02655:Vmn2r4
|
APN |
3 |
64,305,886 (GRCm39) |
missense |
probably damaging |
0.97 |
|
IGL02666:Vmn2r4
|
APN |
3 |
64,296,433 (GRCm39) |
missense |
probably benign |
0.10 |
|
IGL02902:Vmn2r4
|
APN |
3 |
64,314,337 (GRCm39) |
missense |
probably benign |
0.22 |
|
IGL03189:Vmn2r4
|
APN |
3 |
64,296,589 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
IGL03250:Vmn2r4
|
APN |
3 |
64,314,063 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03271:Vmn2r4
|
APN |
3 |
64,305,850 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0310:Vmn2r4
|
UTSW |
3 |
64,296,855 (GRCm39) |
nonsense |
probably null |
|
|
R0504:Vmn2r4
|
UTSW |
3 |
64,296,784 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1546:Vmn2r4
|
UTSW |
3 |
64,314,309 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1863:Vmn2r4
|
UTSW |
3 |
64,314,410 (GRCm39) |
missense |
probably benign |
0.33 |
|
R1873:Vmn2r4
|
UTSW |
3 |
64,298,479 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R1939:Vmn2r4
|
UTSW |
3 |
64,305,976 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2103:Vmn2r4
|
UTSW |
3 |
64,322,704 (GRCm39) |
missense |
possibly damaging |
0.48 |
|
R3083:Vmn2r4
|
UTSW |
3 |
64,296,788 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3687:Vmn2r4
|
UTSW |
3 |
64,296,896 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R3707:Vmn2r4
|
UTSW |
3 |
64,296,895 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R3963:Vmn2r4
|
UTSW |
3 |
64,322,572 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4428:Vmn2r4
|
UTSW |
3 |
64,322,590 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4710:Vmn2r4
|
UTSW |
3 |
64,317,201 (GRCm39) |
critical splice donor site |
probably null |
|
|
R4737:Vmn2r4
|
UTSW |
3 |
64,317,384 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4767:Vmn2r4
|
UTSW |
3 |
64,298,397 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4776:Vmn2r4
|
UTSW |
3 |
64,296,082 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R4834:Vmn2r4
|
UTSW |
3 |
64,317,484 (GRCm39) |
missense |
probably benign |
0.40 |
|
R4893:Vmn2r4
|
UTSW |
3 |
64,313,676 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R4908:Vmn2r4
|
UTSW |
3 |
64,296,476 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
R5049:Vmn2r4
|
UTSW |
3 |
64,306,019 (GRCm39) |
splice site |
probably null |
|
|
R5092:Vmn2r4
|
UTSW |
3 |
64,298,373 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5234:Vmn2r4
|
UTSW |
3 |
64,305,878 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R5240:Vmn2r4
|
UTSW |
3 |
64,314,358 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R5704:Vmn2r4
|
UTSW |
3 |
64,317,370 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5897:Vmn2r4
|
UTSW |
3 |
64,322,687 (GRCm39) |
nonsense |
probably null |
|
|
R5907:Vmn2r4
|
UTSW |
3 |
64,298,487 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5924:Vmn2r4
|
UTSW |
3 |
64,296,685 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6145:Vmn2r4
|
UTSW |
3 |
64,314,364 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6191:Vmn2r4
|
UTSW |
3 |
64,322,702 (GRCm39) |
missense |
probably benign |
0.34 |
|
R6192:Vmn2r4
|
UTSW |
3 |
64,322,699 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6207:Vmn2r4
|
UTSW |
3 |
64,313,926 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6457:Vmn2r4
|
UTSW |
3 |
64,317,378 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6533:Vmn2r4
|
UTSW |
3 |
64,322,519 (GRCm39) |
missense |
probably benign |
|
|
R6545:Vmn2r4
|
UTSW |
3 |
64,313,777 (GRCm39) |
missense |
possibly damaging |
0.50 |
|
R6594:Vmn2r4
|
UTSW |
3 |
64,296,731 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7049:Vmn2r4
|
UTSW |
3 |
64,296,550 (GRCm39) |
missense |
probably benign |
0.14 |
|
R7150:Vmn2r4
|
UTSW |
3 |
64,305,898 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7187:Vmn2r4
|
UTSW |
3 |
64,322,681 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7363:Vmn2r4
|
UTSW |
3 |
64,314,432 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7477:Vmn2r4
|
UTSW |
3 |
64,305,850 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7675:Vmn2r4
|
UTSW |
3 |
64,322,657 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7858:Vmn2r4
|
UTSW |
3 |
64,317,226 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7888:Vmn2r4
|
UTSW |
3 |
64,313,943 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8678:Vmn2r4
|
UTSW |
3 |
64,314,391 (GRCm39) |
missense |
probably benign |
|
|
R8743:Vmn2r4
|
UTSW |
3 |
64,317,247 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R8841:Vmn2r4
|
UTSW |
3 |
64,314,058 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R9671:Vmn2r4
|
UTSW |
3 |
64,317,271 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9778:Vmn2r4
|
UTSW |
3 |
64,322,497 (GRCm39) |
missense |
probably benign |
0.15 |
|
X0019:Vmn2r4
|
UTSW |
3 |
64,314,057 (GRCm39) |
missense |
probably damaging |
0.99 |
|
| Predicted Primers |
PCR Primer
(F):5'- AAAAGCCCAGTGCCAGAGTGAC -3'
(R):5'- GGTATTAACCTGATGTGTGCCAGTCC -3'
Sequencing Primer
(F):5'- AGTGCCAGAGTGACCTGAC -3'
(R):5'- CAACTGAATCTGTCACATCATCTG -3'
|
| Posted On |
2014-04-13 |
Incidental Mutation