Mutagenetix > Incidental Mutation

Incidental Mutation 'R1581:Vmn2r5'

171380

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r5

Ensembl Gene

ENSMUSG00000068999

Gene Name

vomeronasal 2, receptor 5

Synonyms

EG667060

MMRRC Submission

039618-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.145) question?

Stock #

R1581 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

64398242-64417156 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 64398640 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 780 (C780R)

Ref Sequence

ENSEMBL: ENSMUSP00000135592 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170270] [ENSMUST00000177184]

AlphaFold

K7N788

Predicted Effect

probably damaging
Transcript: ENSMUST00000170270
AA Change: C693R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000131925
Gene: ENSMUSG00000068999
AA Change: C693R

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	9.9e-78	PFAM
Pfam:NCD3G	458	511	8.5e-18	PFAM
Pfam:7tm_3	542	779	2.3e-74	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000177184
AA Change: C780R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135592
Gene: ENSMUSG00000068999
AA Change: C780R

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	86	503	1.8e-80	PFAM
Pfam:NCD3G	545	598	1e-14	PFAM
Pfam:7tm_3	631	865	1.4e-45	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 96.2%
20x: 92.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd2	T	C	15: 91,063,347 (GRCm39)	E447G	probably benign	Het
Actn4	T	C	7: 28,598,071 (GRCm39)	T510A	probably benign	Het
Adgrb3	A	G	1: 25,133,153 (GRCm39)	M1311T	possibly damaging	Het
Arfgef1	C	T	1: 10,270,103 (GRCm39)	A349T	probably benign	Het
Auh	G	A	13: 52,989,532 (GRCm39)	P308L	probably benign	Het
Bicdl1	G	T	5: 115,789,326 (GRCm39)		probably benign	Het
Bmyc	T	C	2: 25,597,346 (GRCm39)	S137P	probably damaging	Het
Camsap3	C	T	8: 3,654,708 (GRCm39)	R782C	probably damaging	Het
Casc3	C	T	11: 98,713,644 (GRCm39)	T292I	possibly damaging	Het
Chmp7	C	T	14: 69,956,899 (GRCm39)	M336I	probably benign	Het
Cibar1	T	A	4: 12,155,745 (GRCm39)		probably null	Het
Cnnm2	A	G	19: 46,751,562 (GRCm39)	T451A	probably damaging	Het
Eed	T	C	7: 89,629,676 (GRCm39)	K20E	possibly damaging	Het
Eps15	T	A	4: 109,220,383 (GRCm39)	M180K	probably benign	Het
Esr1	T	C	10: 4,947,905 (GRCm39)	I486T	probably damaging	Het
Etnppl	A	G	3: 130,422,393 (GRCm39)	I207V	possibly damaging	Het
Fancg	G	A	4: 43,007,039 (GRCm39)	P246L	probably damaging	Het
Fcrl1	G	A	3: 87,293,030 (GRCm39)	C249Y	possibly damaging	Het
Foxred2	A	G	15: 77,839,961 (GRCm39)	F110L	possibly damaging	Het
Fsip2	A	T	2: 82,816,626 (GRCm39)	N4120Y	probably damaging	Het
Gm4884	C	T	7: 40,693,255 (GRCm39)	S408L	probably benign	Het
Gm9476	T	C	10: 100,142,474 (GRCm39)		noncoding transcript	Het
Gria1	T	C	11: 57,127,836 (GRCm39)		probably null	Het
H6pd	T	A	4: 150,066,971 (GRCm39)	I472F	possibly damaging	Het
Hydin	A	T	8: 111,137,092 (GRCm39)	M632L	probably benign	Het
Hyou1	C	T	9: 44,300,167 (GRCm39)	P819S	probably damaging	Het
Il6st	A	G	13: 112,618,075 (GRCm39)	E163G	probably damaging	Het
Kcnk1	T	C	8: 126,722,278 (GRCm39)	V27A	possibly damaging	Het
Kdelr3	T	C	15: 79,407,114 (GRCm39)		probably null	Het
Klk1b22	A	G	7: 43,765,399 (GRCm39)	N117S	possibly damaging	Het
Klrh1	T	C	6: 129,752,796 (GRCm39)	D3G	probably benign	Het
Lpp	C	A	16: 24,500,591 (GRCm39)	C134*	probably null	Het
Lrrc37a	G	A	11: 103,347,843 (GRCm39)	R2951*	probably null	Het
Luzp2	T	A	7: 54,899,238 (GRCm39)	D285E	possibly damaging	Het
Ly75	C	T	2: 60,158,237 (GRCm39)	R1016H	probably damaging	Het
Mesp2	T	A	7: 79,462,289 (GRCm39)	S282T	possibly damaging	Het
Nav3	T	C	10: 109,659,289 (GRCm39)	D776G	probably damaging	Het
Nr2e1	T	C	10: 42,443,964 (GRCm39)	T253A	probably benign	Het
Nup214	C	T	2: 31,924,478 (GRCm39)	S1669F	probably damaging	Het
Or1r1	A	T	11: 73,875,347 (GRCm39)	L29H	probably damaging	Het
Padi6	T	C	4: 140,463,147 (GRCm39)	Y146C	probably damaging	Het
Pank4	C	T	4: 155,059,108 (GRCm39)	R414W	probably damaging	Het
Pclo	T	G	5: 14,571,296 (GRCm39)	I227S	probably benign	Het
Plppr4	A	G	3: 117,121,915 (GRCm39)	V221A	possibly damaging	Het
Pradc1	G	A	6: 85,425,568 (GRCm39)	R25C	probably damaging	Het
Rfwd3	C	T	8: 112,014,874 (GRCm39)	R326Q	probably damaging	Het
Rnd2	A	G	11: 101,362,022 (GRCm39)	T192A	probably benign	Het
Rtbdn	A	G	8: 85,681,695 (GRCm39)	E131G	probably benign	Het
Ryr2	A	G	13: 11,809,449 (GRCm39)	V792A	probably benign	Het
Sacs	G	T	14: 61,451,128 (GRCm39)	Q4391H	probably damaging	Het
Scd2	A	G	19: 44,286,538 (GRCm39)	S123G	probably benign	Het
Septin9	A	G	11: 117,181,421 (GRCm39)	R74G	probably damaging	Het
Sipa1l2	T	A	8: 126,218,356 (GRCm39)	Q327L	probably damaging	Het
Skor1	T	C	9: 63,053,505 (GRCm39)	T127A	probably damaging	Het
Sphk2	A	G	7: 45,362,920 (GRCm39)	V57A	probably damaging	Het
Tfec	A	T	6: 16,844,243 (GRCm39)	D101E	probably damaging	Het
Tmem43	T	A	6: 91,455,717 (GRCm39)	H109Q	probably benign	Het
Tmem67	C	A	4: 12,047,814 (GRCm39)	S839I	probably damaging	Het
Trpv5	T	C	6: 41,630,074 (GRCm39)	Y672C	probably damaging	Het
Ttc39d	A	C	17: 80,523,913 (GRCm39)	S191R	probably benign	Het
Ttll1	C	T	15: 83,380,478 (GRCm39)	V296M	probably damaging	Het
Upp2	A	C	2: 58,664,177 (GRCm39)	K130T	possibly damaging	Het
Wars1	C	A	12: 108,841,635 (GRCm39)	E171*	probably null	Het
Zeb2	A	T	2: 44,887,012 (GRCm39)	S637T	probably damaging	Het
Zfp27	T	A	7: 29,595,549 (GRCm39)	T139S	possibly damaging	Het
Zfp941	A	T	7: 140,392,033 (GRCm39)	L442Q	probably benign	Het

Other mutations in Vmn2r5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00742:Vmn2r5	APN	3	64,398,834 (GRCm39)	missense	possibly damaging	0.67
IGL01139:Vmn2r5	APN	3	64,398,826 (GRCm39)	missense	probably benign	0.28
IGL03293:Vmn2r5	APN	3	64,398,747 (GRCm39)	missense	probably benign	0.01
PIT4581001:Vmn2r5	UTSW	3	64,411,348 (GRCm39)	nonsense	probably null
R0063:Vmn2r5	UTSW	3	64,411,221 (GRCm39)	missense	probably benign	0.01
R0142:Vmn2r5	UTSW	3	64,400,009 (GRCm39)	missense	probably damaging	1.00
R0193:Vmn2r5	UTSW	3	64,398,951 (GRCm39)	missense	possibly damaging	0.91
R0219:Vmn2r5	UTSW	3	64,411,734 (GRCm39)	missense	probably damaging	0.99
R0313:Vmn2r5	UTSW	3	64,411,248 (GRCm39)	missense	probably benign
R0620:Vmn2r5	UTSW	3	64,411,235 (GRCm39)	nonsense	probably null
R0726:Vmn2r5	UTSW	3	64,411,186 (GRCm39)	missense	probably benign	0.01
R1073:Vmn2r5	UTSW	3	64,398,726 (GRCm39)	nonsense	probably null
R1549:Vmn2r5	UTSW	3	64,411,421 (GRCm39)	missense	probably damaging	1.00
R1624:Vmn2r5	UTSW	3	64,417,116 (GRCm39)	missense	probably benign	0.19
R1973:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1975:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1977:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R2189:Vmn2r5	UTSW	3	64,417,014 (GRCm39)	missense	probably benign	0.27
R2484:Vmn2r5	UTSW	3	64,411,392 (GRCm39)	missense	possibly damaging	0.94
R4573:Vmn2r5	UTSW	3	64,411,339 (GRCm39)	missense	probably damaging	0.98
R5243:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5384:Vmn2r5	UTSW	3	64,416,931 (GRCm39)	missense	probably benign	0.02
R5385:Vmn2r5	UTSW	3	64,416,931 (GRCm39)	missense	probably benign	0.02
R5587:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5588:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5589:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5590:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5739:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R6113:Vmn2r5	UTSW	3	64,398,820 (GRCm39)	missense	probably benign	0.03
R6739:Vmn2r5	UTSW	3	64,398,637 (GRCm39)	missense	probably damaging	0.97
R7106:Vmn2r5	UTSW	3	64,399,104 (GRCm39)	missense	probably benign	0.05
R7304:Vmn2r5	UTSW	3	64,411,671 (GRCm39)	missense	probably damaging	0.97
R7402:Vmn2r5	UTSW	3	64,403,176 (GRCm39)	missense	probably benign	0.05
R7477:Vmn2r5	UTSW	3	64,399,060 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r5	UTSW	3	64,411,825 (GRCm39)	missense	probably damaging	1.00
R7678:Vmn2r5	UTSW	3	64,416,943 (GRCm39)	missense	probably benign
R7874:Vmn2r5	UTSW	3	64,398,453 (GRCm39)	missense	probably damaging	1.00
R8110:Vmn2r5	UTSW	3	64,398,709 (GRCm39)	missense	probably benign	0.00
R8223:Vmn2r5	UTSW	3	64,398,726 (GRCm39)	nonsense	probably null
R8767:Vmn2r5	UTSW	3	64,415,103 (GRCm39)	missense	possibly damaging	0.79
R8896:Vmn2r5	UTSW	3	64,411,203 (GRCm39)	missense	probably benign	0.00
R8948:Vmn2r5	UTSW	3	64,398,522 (GRCm39)	missense	probably damaging	1.00
R8950:Vmn2r5	UTSW	3	64,398,522 (GRCm39)	missense	probably damaging	1.00
R8962:Vmn2r5	UTSW	3	64,398,564 (GRCm39)	missense	probably damaging	1.00
R9012:Vmn2r5	UTSW	3	64,411,915 (GRCm39)	missense	probably damaging	1.00
R9109:Vmn2r5	UTSW	3	64,411,411 (GRCm39)	missense	possibly damaging	0.58
R9126:Vmn2r5	UTSW	3	64,399,159 (GRCm39)	missense	probably benign	0.13
R9192:Vmn2r5	UTSW	3	64,398,938 (GRCm39)	nonsense	probably null
R9221:Vmn2r5	UTSW	3	64,411,721 (GRCm39)	nonsense	probably null
R9375:Vmn2r5	UTSW	3	64,411,316 (GRCm39)	missense	probably damaging	1.00
R9529:Vmn2r5	UTSW	3	64,400,018 (GRCm39)	missense	probably benign	0.06
Z1088:Vmn2r5	UTSW	3	64,416,963 (GRCm39)	missense	probably benign	0.02
Z1177:Vmn2r5	UTSW	3	64,398,472 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTGGTGCTCAAGTAAGCAGGGAC -3'
(R):5'- CCGAGAGCTAAGCTTCCTCATTCAG -3'

Sequencing Primer
(F):5'- CAGGGACAAAAGAGATCCAGAC -3'
(R):5'- CCATGCTGTTCATAGGCAAG -3'

Posted On

2014-04-13