Mutagenetix > Incidental Mutation

Incidental Mutation 'R0226:Gpr155'

172379

Institutional Source

Beutler Lab

Gene Symbol

Gpr155

Ensembl Gene

ENSMUSG00000041762

Gene Name

G protein-coupled receptor 155

Synonyms

PGR22, 1110017O10Rik, DEPDC3, F730029F15Rik

MMRRC Submission

038471-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0226 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

73341506-73386572 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 73367592 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 395 (V395I)

Ref Sequence

ENSEMBL: ENSMUSP00000107675 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076463] [ENSMUST00000112043] [ENSMUST00000112044]

AlphaFold

A2AWR3

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000048504

SMART Domains

Protein: ENSMUSP00000037772
Gene: ENSMUSG00000041762

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	1.9e-16	PFAM
Pfam:Mem_trans	187	360	4.9e-12	PFAM
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000076463
AA Change: V423I

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: V423I

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112043
AA Change: V423I

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: V423I

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112044
AA Change: V395I

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: V395I

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
Pfam:Mem_trans	187	360	8.3e-11	PFAM
transmembrane domain	382	404	N/A	INTRINSIC
transmembrane domain	414	436	N/A	INTRINSIC
transmembrane domain	449	471	N/A	INTRINSIC
transmembrane domain	491	510	N/A	INTRINSIC
transmembrane domain	629	651	N/A	INTRINSIC
transmembrane domain	661	683	N/A	INTRINSIC
DEP	731	805	8.28e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129184

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138573

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150705

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184503

Meta Mutation Damage Score

0.0660

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.4%

Validation Efficiency

100% (98/98)

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700129C05Rik	G	T	14: 59,142,120	T54K	possibly damaging	Het
2210408I21Rik	A	T	13: 77,303,425	E876V	possibly damaging	Het
Aasdh	A	T	5: 76,902,002	L49Q	probably damaging	Het
Abca8b	T	C	11: 109,957,018		probably null	Het
Ablim1	A	T	19: 57,043,870	L556Q	probably damaging	Het
Afdn	A	G	17: 13,899,146	T1700A	probably benign	Het
Agl	G	A	3: 116,752,071	R1359C	probably damaging	Het
Agpat3	T	A	10: 78,278,029	H275L	possibly damaging	Het
Ahcyl1	A	T	3: 107,670,270	C180*	probably null	Het
Aim2	A	G	1: 173,462,333		probably benign	Het
Angpt1	T	C	15: 42,468,235	N320S	probably benign	Het
Ankrd52	T	A	10: 128,389,858		probably null	Het
Ap1g1	C	T	8: 109,855,062	S654L	probably benign	Het
Bpifa1	T	A	2: 154,146,057	S173R	probably benign	Het
Brd8	T	C	18: 34,603,894		probably benign	Het
Btbd9	C	T	17: 30,274,942	D492N	possibly damaging	Het
C1qtnf2	A	G	11: 43,490,843	T161A	probably benign	Het
Car6	T	C	4: 150,187,508	Y228C	probably damaging	Het
Ccdc149	A	G	5: 52,400,217	L273P	probably damaging	Het
Cit	G	A	5: 115,984,840	R1405Q	probably damaging	Het
Cox17	T	C	16: 38,349,276	L48P	probably damaging	Het
Cttn	A	G	7: 144,441,852		probably benign	Het
Cyp4f18	T	C	8: 71,989,775		probably benign	Het
Dtnbp1	A	G	13: 44,923,193	L175P	probably damaging	Het
Efl1	T	C	7: 82,693,011		probably benign	Het
Fbn1	C	T	2: 125,320,910	R2152Q	possibly damaging	Het
Fignl1	A	T	11: 11,801,061	S665T	probably benign	Het
Gm884	A	T	11: 103,603,241	F663L	probably benign	Het
Gm9843	A	T	16: 76,403,561		noncoding transcript	Het
Greb1l	T	C	18: 10,522,076		probably benign	Het
Hdgfl1	A	T	13: 26,769,996	H31Q	probably benign	Het
Heatr1	T	C	13: 12,410,562	S628P	probably damaging	Het
Hivep2	A	G	10: 14,129,712	T685A	probably benign	Het
Hmgcl	T	G	4: 135,958,728	V168G	probably damaging	Het
Itch	T	C	2: 155,199,394	I454T	probably benign	Het
Itih2	T	C	2: 10,115,299	D309G	possibly damaging	Het
Kcmf1	T	C	6: 72,842,952	I304V	probably benign	Het
Kcnh1	G	A	1: 192,276,804	W222*	probably null	Het
Kcnh1	G	T	1: 192,276,805	W222C	probably damaging	Het
Kif24	T	A	4: 41,414,939	K287*	probably null	Het
Lrig3	A	G	10: 125,972,117		probably benign	Het
Lrp2	A	T	2: 69,537,563	C202S	probably null	Het
Lrrn2	A	G	1: 132,937,820	N208D	probably damaging	Het
Mcm5	C	T	8: 75,126,252	T664I	possibly damaging	Het
Mfsd10	A	G	5: 34,634,446	L365S	probably benign	Het
Mfsd6	A	G	1: 52,658,690		probably benign	Het
Mgat4e	A	G	1: 134,541,103	V401A	probably benign	Het
Mllt3	C	A	4: 87,840,732	V360L	probably benign	Het
Mrm1	G	A	11: 84,819,170	A68V	possibly damaging	Het
Myo19	A	G	11: 84,897,732		probably benign	Het
Myo3b	A	G	2: 70,217,166	T311A	probably benign	Het
Myo5b	T	C	18: 74,742,180	F1552L	probably benign	Het
Myo7b	C	T	18: 31,972,896	V1353I	probably benign	Het
Myo9b	T	C	8: 71,353,832	S1512P	probably damaging	Het
Nanos3	C	T	8: 84,176,134	R133Q	probably damaging	Het
Osbpl3	A	G	6: 50,353,008	W63R	probably damaging	Het
Pcdh17	T	C	14: 84,448,201	S703P	probably damaging	Het
Pclo	T	C	5: 14,765,223	I1231T	probably damaging	Het
Pex16	A	C	2: 92,375,687		probably benign	Het
Pfkl	T	C	10: 77,992,534	N399S	probably benign	Het
Pkhd1l1	G	A	15: 44,526,784	R1432K	possibly damaging	Het
Prdm1	T	A	10: 44,456,696	T106S	probably benign	Het
Prrc1	A	G	18: 57,363,291	M105V	probably benign	Het
Psg26	A	G	7: 18,483,958	C12R	possibly damaging	Het
Rnf43	A	T	11: 87,731,437	S455C	probably damaging	Het
Ryr2	T	C	13: 11,772,556	K977R	probably damaging	Het
Sart1	C	T	19: 5,381,122		probably benign	Het
Sec14l5	A	G	16: 5,180,303	S509G	probably benign	Het
Sin3b	A	T	8: 72,744,508	E361V	probably benign	Het
Slc35e3	C	T	10: 117,740,890	E179K	possibly damaging	Het
Sntb2	T	C	8: 107,001,583	S388P	probably damaging	Het
Srms	A	G	2: 181,212,382	S131P	probably benign	Het
Stxbp5	T	C	10: 9,866,698		probably benign	Het
Taar2	T	C	10: 23,941,063	V167A	probably damaging	Het
Taar2	G	A	10: 23,941,495	R311H	probably benign	Het
Thsd7a	A	G	6: 12,321,900	Y1516H	possibly damaging	Het
Tlk1	T	A	2: 70,714,169		probably benign	Het
Tmem173	A	T	18: 35,739,088	F120L	probably benign	Het
Tnfaip3	T	C	10: 19,002,747	K771R	probably damaging	Het
Treml1	C	T	17: 48,360,458	L124F	probably damaging	Het
Ttn	G	T	2: 76,780,797	Q9137K	possibly damaging	Het
Unkl	T	A	17: 25,230,711	I469N	probably damaging	Het
Vmn1r173	G	T	7: 23,703,083	V248L	possibly damaging	Het
Vps35	T	C	8: 85,273,575	Q474R	probably damaging	Het
Wdr17	T	A	8: 54,663,008	T580S	probably benign	Het
Xpnpep1	T	C	19: 53,010,152	K222E	probably benign	Het
Ylpm1	A	G	12: 85,049,737	T1446A	probably benign	Het
Zfp277	A	G	12: 40,364,162	L228S	possibly damaging	Het
Zfp941	T	A	7: 140,813,275	K57M	probably damaging	Het
Zmpste24	A	T	4: 121,081,209	S244R	probably benign	Het

Other mutations in Gpr155

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00731:Gpr155	APN	2	73362613	missense	probably damaging	1.00
IGL01432:Gpr155	APN	2	73351885	missense	possibly damaging	0.51
IGL01528:Gpr155	APN	2	73362423	critical splice donor site	probably null
IGL01718:Gpr155	APN	2	73382232	missense	probably benign
IGL01733:Gpr155	APN	2	73353612	splice site	probably null
IGL03342:Gpr155	APN	2	73349678	missense	probably damaging	1.00
PIT1430001:Gpr155	UTSW	2	73370138	missense	probably benign	0.08
PIT4810001:Gpr155	UTSW	2	73348263	missense	probably benign	0.08
R0399:Gpr155	UTSW	2	73370002	missense	possibly damaging	0.91
R0445:Gpr155	UTSW	2	73370144	splice site	probably benign
R1570:Gpr155	UTSW	2	73370038	missense	possibly damaging	0.87
R1598:Gpr155	UTSW	2	73370090	missense	probably damaging	0.98
R1647:Gpr155	UTSW	2	73364164	splice site	probably null
R1648:Gpr155	UTSW	2	73364164	splice site	probably null
R1756:Gpr155	UTSW	2	73367577	missense	probably benign	0.00
R1760:Gpr155	UTSW	2	73381935	missense	probably damaging	1.00
R2033:Gpr155	UTSW	2	73348182	missense	probably benign	0.18
R2044:Gpr155	UTSW	2	73373633	missense	probably damaging	1.00
R2145:Gpr155	UTSW	2	73356658	missense	probably benign	0.01
R2172:Gpr155	UTSW	2	73382127	missense	probably benign	0.00
R2274:Gpr155	UTSW	2	73348135	critical splice donor site	probably null
R3878:Gpr155	UTSW	2	73368392	nonsense	probably null
R3924:Gpr155	UTSW	2	73370076	missense	probably damaging	1.00
R4910:Gpr155	UTSW	2	73367538	nonsense	probably null
R4950:Gpr155	UTSW	2	73382185	missense	probably benign
R5337:Gpr155	UTSW	2	73348248	missense	probably benign	0.32
R5830:Gpr155	UTSW	2	73370089	missense	possibly damaging	0.93
R5887:Gpr155	UTSW	2	73343718	nonsense	probably null
R5929:Gpr155	UTSW	2	73373667	nonsense	probably null
R6293:Gpr155	UTSW	2	73373997	missense	possibly damaging	0.47
R6553:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R6585:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R7003:Gpr155	UTSW	2	73343617	missense	probably damaging	0.99
R7353:Gpr155	UTSW	2	73367491	nonsense	probably null
R7506:Gpr155	UTSW	2	73368339	missense	probably damaging	0.97
R7631:Gpr155	UTSW	2	73382947	intron	probably benign
R7753:Gpr155	UTSW	2	73382206	missense	probably benign	0.27
R7810:Gpr155	UTSW	2	73381952	missense	probably damaging	0.99
R7813:Gpr155	UTSW	2	73381985	nonsense	probably null
R7815:Gpr155	UTSW	2	73362560	missense	probably benign
R7873:Gpr155	UTSW	2	73343590	missense	possibly damaging	0.51
R8506:Gpr155	UTSW	2	73343462	missense	probably damaging	0.99
R8680:Gpr155	UTSW	2	73343695	missense	probably damaging	0.99
R8856:Gpr155	UTSW	2	73373649	missense	probably benign	0.27
R8872:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R9116:Gpr155	UTSW	2	73373765	missense	possibly damaging	0.78
R9683:Gpr155	UTSW	2	73362436	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CACCTGGTGTCTTCAAGGGGAAAC -3'
(R):5'- AAGACTGTCTCGGCGACTGAAAC -3'

Sequencing Primer
(F):5'- tgtgtgtgtctgtgtgtttg -3'
(R):5'- AACTTACTCCCCAGCATGTGG -3'

Posted On

2014-04-22