Mutagenetix > Incidental Mutations

Incidental Mutation 'R1227:Zfp108'

172408

Institutional Source

Beutler Lab

Gene Symbol

Zfp108

Ensembl Gene

ENSMUSG00000030486

Gene Name

zinc finger protein 108

Synonyms

MMRRC Submission

039296-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R1227 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

24254794-24262445 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 24260460 bp

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 159 (W159R)

Ref Sequence

ENSEMBL: ENSMUSP00000072496 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072713] [ENSMUST00000205982] [ENSMUST00000206777]

Predicted Effect

probably benign
Transcript: ENSMUST00000072713
AA Change: W159R

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000072496
Gene: ENSMUSG00000030486
AA Change: W159R

Domain	Start	End	E-Value	Type
KRAB	8	63	7.94e-18	SMART
low complexity region	140	153	N/A	INTRINSIC
ZnF_C2H2	281	303	1.33e-1	SMART
ZnF_C2H2	309	331	1.69e-3	SMART
ZnF_C2H2	337	359	1.3e-4	SMART
ZnF_C2H2	365	387	2.71e-2	SMART
ZnF_C2H2	393	415	5.14e-3	SMART
ZnF_C2H2	421	443	1.87e-5	SMART
ZnF_C2H2	449	471	3.44e-4	SMART
ZnF_C2H2	477	497	1.08e1	SMART
ZnF_C2H2	503	525	3.89e-3	SMART
ZnF_C2H2	531	553	2.09e-3	SMART
ZnF_C2H2	559	581	4.61e-5	SMART
ZnF_C2H2	587	609	7.9e-4	SMART
ZnF_C2H2	615	637	1.67e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000205982
AA Change: W159R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000206777
AA Change: W159R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.5%
20x: 89.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 20 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd42	T	A	7: 92,605,300	H367L	possibly damaging	Het
Arvcf	C	T	16: 18,389,304	R43C	probably benign	Het
Camk1g	T	C	1: 193,347,433	E453G	possibly damaging	Het
Cmya5	A	T	13: 93,094,446	L1378Q	probably damaging	Het
Cplx1	T	C	5: 108,525,396	D53G	possibly damaging	Het
Ddx31	A	G	2: 28,857,175	E222G	probably damaging	Het
Dync1h1	G	A	12: 110,636,509	E2195K	probably benign	Het
Fermt2	A	G	14: 45,459,990	S635P	probably benign	Het
Gls2	T	C	10: 128,199,664	S104P	probably damaging	Het
Gm13023	A	G	4: 143,793,564	H126R	probably benign	Het
Hnrnpul2	T	G	19: 8,823,237	S226A	possibly damaging	Het
Klk5	T	C	7: 43,847,246		probably null	Het
Nuak1	T	A	10: 84,440,309	T17S	probably benign	Het
Olfr1453	T	C	19: 13,027,853	M159V	probably benign	Het
Olfr474	C	A	7: 107,955,052	S137Y	probably damaging	Het
Prag1	G	A	8: 36,139,951	E949K	probably damaging	Het
Rasal1	T	C	5: 120,670,307	L468P	probably damaging	Het
Sez6l	C	A	5: 112,473,464	C248F	probably damaging	Het
Sstr2	T	C	11: 113,624,885	I210T	probably damaging	Het
Tbc1d2	C	T	4: 46,620,629	G394S	probably benign	Het

Other mutations in Zfp108

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00591:Zfp108	APN	7	24261486	missense	possibly damaging	0.82
IGL01392:Zfp108	APN	7	24258447	splice site	probably benign
R0123:Zfp108	UTSW	7	24260467	missense	probably benign	0.00
R0126:Zfp108	UTSW	7	24260724	missense	probably benign	0.01
R0134:Zfp108	UTSW	7	24260467	missense	probably benign	0.00
R0243:Zfp108	UTSW	7	24261783	missense	possibly damaging	0.81
R1464:Zfp108	UTSW	7	24260548	missense	probably benign	0.00
R1464:Zfp108	UTSW	7	24260548	missense	probably benign	0.00
R1731:Zfp108	UTSW	7	24258539	missense	possibly damaging	0.75
R1739:Zfp108	UTSW	7	24261310	missense	probably damaging	1.00
R1751:Zfp108	UTSW	7	24261896	missense	probably damaging	1.00
R3713:Zfp108	UTSW	7	24261845	nonsense	probably null
R3839:Zfp108	UTSW	7	24260556	missense	probably benign	0.01
R3919:Zfp108	UTSW	7	24260832	missense	probably damaging	0.99
R3922:Zfp108	UTSW	7	24261348	missense	probably damaging	1.00
R4707:Zfp108	UTSW	7	24260412	missense	probably benign	0.08
R4912:Zfp108	UTSW	7	24261314	missense	probably damaging	1.00
R4965:Zfp108	UTSW	7	24260148	missense	probably benign
R4989:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5014:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5163:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5183:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5184:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5185:Zfp108	UTSW	7	24260738	missense	probably benign	0.00
R5453:Zfp108	UTSW	7	24261264	missense	probably damaging	1.00
R5600:Zfp108	UTSW	7	24260586	missense	probably benign	0.00
R6494:Zfp108	UTSW	7	24261357	missense	probably damaging	1.00
R6601:Zfp108	UTSW	7	24261394	missense	probably damaging	0.98
R6735:Zfp108	UTSW	7	24261772	missense	probably damaging	1.00
R7646:Zfp108	UTSW	7	24261415	missense	probably damaging	1.00
R7732:Zfp108	UTSW	7	24261527	missense	probably benign	0.00
RF019:Zfp108	UTSW	7	24261607	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GAGAACTTAGCCCTTCAATGTCCCC -3'
(R):5'- TGATGACTTGATCACAGTCACCGC -3'

Sequencing Primer
(F):5'- TATATTCCCTTCAGGAGGCCAGAG -3'
(R):5'- ACAGTCACCGCTGCTGG -3'

Posted On

2014-04-24