Incidental Mutation 'R1323:H2af-ps2'
ID 172464
Institutional Source Beutler Lab
Gene Symbol H2af-ps2
Ensembl Gene ENSMUSG00000091383
Gene Name H2A histone family, pseudogene 2
Synonyms EG667728, Hist1h2al
MMRRC Submission 039389-MU
Accession Numbers
Essential gene? Not available question?
Stock # R1323 (G1)
Quality Score 205
Status Not validated
Chromosome 13
Chromosomal Location 51356763-51357101 bp(-) (GRCm39)
Type of Mutation unclassified
DNA Base Change (assembly) T to G at 51357100 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Gene Model predicted gene model for transcript(s):
AlphaFold no structure available at present
Predicted Effect noncoding transcript
Transcript: ENSMUST00000091708
SMART Domains Protein: ENSMUSP00000089300
Gene: ENSMUSG00000091383

DomainStartEndE-ValueType
H2A 1 118 3.71e-80 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000196168
Predicted Effect noncoding transcript
Transcript: ENSMUST00000222129
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.0%
  • 10x: 95.1%
  • 20x: 88.7%
Validation Efficiency
MGI Phenotype FUNCTION: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is a histone pseudogene found on chromosome 13. [provided by RefSeq, Oct 2015]
Allele List at MGI
Other mutations in this stock
Total: 23 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 C A 11: 9,240,937 (GRCm39) F933L possibly damaging Het
Abcc8 T G 7: 45,766,786 (GRCm39) Q998P probably benign Het
Akr1e1 C T 13: 4,657,547 (GRCm39) G17E probably damaging Het
Ankrd44 A G 1: 54,805,609 (GRCm39) probably benign Het
D630045J12Rik T C 6: 38,125,443 (GRCm39) I1524V probably damaging Het
Elovl1 T C 4: 118,288,851 (GRCm39) L103P possibly damaging Het
Emsy T C 7: 98,259,864 (GRCm39) probably benign Het
Fam171a2 T G 11: 102,334,951 (GRCm39) D62A probably damaging Het
Firrm C A 1: 163,783,030 (GRCm39) probably benign Het
Frzb G A 2: 80,243,720 (GRCm39) P320S probably benign Het
Fsip2 G T 2: 82,816,096 (GRCm39) G3943V probably damaging Het
Ftdc1 G A 16: 58,437,278 (GRCm39) P10L possibly damaging Het
Grm8 A G 6: 28,125,973 (GRCm39) L51P probably damaging Het
Hnf4g T C 3: 3,699,281 (GRCm39) S4P possibly damaging Het
Megf8 T A 7: 25,059,527 (GRCm39) probably null Het
Mtnr1b A G 9: 15,774,432 (GRCm39) F209S probably damaging Het
Nckipsd G A 9: 108,689,778 (GRCm39) R313Q probably damaging Het
Nrbp1 T A 5: 31,403,157 (GRCm39) I210N probably damaging Het
Paqr5 C T 9: 61,868,810 (GRCm39) probably null Het
S100a14 T C 3: 90,435,043 (GRCm39) V18A probably damaging Het
Sycp2 A T 2: 177,989,414 (GRCm39) S1441R possibly damaging Het
Vmn2r101 G A 17: 19,832,313 (GRCm39) D770N probably damaging Het
Zfp600 A G 4: 146,133,261 (GRCm39) Y643C probably damaging Het
Other mutations in H2af-ps2
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1323:H2af-ps2 UTSW 13 51,357,100 (GRCm39) unclassified noncoding transcript
Predicted Primers PCR Primer
(F):5'- GGTTATATCACAGAGACGCGGGC -3'
(R):5'- CTGAGCAGAAGTAATGGTCACTGGG -3'

Sequencing Primer
(F):5'- TCTTCTTGGGCAGCAGCAC -3'
(R):5'- acctagtgtatgtgggactttg -3'
Posted On 2014-04-24