Mutagenetix > Incidental Mutation

Incidental Mutation 'R1631:Mslnl'

172822

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

039668-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1631 (G1)

Quality Score

214

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 93.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933425L06Rik	C	A	13: 105,082,241 (GRCm38)	Q28K	probably benign	Het
Adamts10	T	A	17: 33,537,342 (GRCm38)	S320T	probably benign	Het
Alkbh2	C	T	5: 114,124,226 (GRCm38)	E148K	probably damaging	Het
Als2	T	C	1: 59,218,067 (GRCm38)	E12G	probably benign	Het
Arhgef10	A	T	8: 14,947,157 (GRCm38)	D321V	probably damaging	Het
Atp8a1	T	G	5: 67,749,052 (GRCm38)		probably null	Het
Avil	A	G	10: 127,010,625 (GRCm38)		probably null	Het
C2cd3	G	A	7: 100,372,497 (GRCm38)		probably null	Het
Col18a1	G	A	10: 77,059,297 (GRCm38)	P1177S	probably damaging	Het
Copb2	T	A	9: 98,580,160 (GRCm38)	F428L	probably benign	Het
Cpa1	A	G	6: 30,640,924 (GRCm38)	E138G	probably damaging	Het
Ctsm	T	C	13: 61,538,435 (GRCm38)	I12V	possibly damaging	Het
Dctn1	A	G	6: 83,197,596 (GRCm38)	Q967R	possibly damaging	Het
Dok2	T	C	14: 70,776,953 (GRCm38)	Y194H	probably damaging	Het
Ezh2	C	T	6: 47,577,658 (GRCm38)	M1I	probably null	Het
Fkbp8	T	A	8: 70,531,632 (GRCm38)	L210Q	probably damaging	Het
Fpr1	T	A	17: 17,877,001 (GRCm38)	Q242L	probably benign	Het
Gal3st2b	T	A	1: 93,940,783 (GRCm38)	D243E	probably damaging	Het
Gm281	C	T	14: 13,829,796 (GRCm38)	E649K	probably damaging	Het
Gm5422	A	G	10: 31,249,806 (GRCm38)		noncoding transcript	Het
Gucy1a2	A	G	9: 3,533,052 (GRCm38)	N84D	probably damaging	Het
Hsd3b5	A	C	3: 98,622,077 (GRCm38)	V79G	probably damaging	Het
Htr6	A	T	4: 139,061,493 (GRCm38)	V417E	probably benign	Het
Ifnar2	G	A	16: 91,391,867 (GRCm38)	V79I	probably benign	Het
Ighv10-1	T	C	12: 114,479,482 (GRCm38)		probably benign	Het
Itpr2	A	G	6: 146,180,290 (GRCm38)	F182L	probably damaging	Het
Kirrel	C	T	3: 87,089,151 (GRCm38)	M380I	probably null	Het
Lama3	A	G	18: 12,407,494 (GRCm38)	Y285C	probably damaging	Het
Lamc2	C	A	1: 153,158,934 (GRCm38)	V108L	possibly damaging	Het
Lrrc14b	A	G	13: 74,361,254 (GRCm38)		probably null	Het
Magi3	T	C	3: 104,051,177 (GRCm38)	T531A	probably benign	Het
Mapre2	A	G	18: 23,832,954 (GRCm38)	Y32C	probably damaging	Het
Med1	C	T	11: 98,155,626 (GRCm38)		probably benign	Het
Olfr1351	A	T	10: 79,017,405 (GRCm38)	I28L	probably benign	Het
Olfr191	G	A	16: 59,086,045 (GRCm38)	T146I	probably benign	Het
Olfr516	A	C	7: 108,845,857 (GRCm38)	L51R	probably damaging	Het
Olfr555	A	G	7: 102,659,201 (GRCm38)	I127V	probably damaging	Het
Pde1b	A	G	15: 103,521,672 (GRCm38)	T143A	probably damaging	Het
Pkhd1	T	C	1: 20,522,897 (GRCm38)	D1664G	probably benign	Het
Plod3	C	T	5: 136,988,993 (GRCm38)	R208W	probably damaging	Het
Pstk	G	A	7: 131,384,542 (GRCm38)	A277T	possibly damaging	Het
Qrich1	T	C	9: 108,534,485 (GRCm38)	V403A	probably damaging	Het
Rad21	C	A	15: 51,970,040 (GRCm38)	V348F	probably damaging	Het
Sacs	T	A	14: 61,210,732 (GRCm38)	L3409*	probably null	Het
Setd4	T	A	16: 93,593,248 (GRCm38)	K98*	probably null	Het
Skint5	A	G	4: 113,483,926 (GRCm38)	V1385A	probably benign	Het
Stam	C	A	2: 14,146,248 (GRCm38)	S472*	probably null	Het
Stx2	A	G	5: 128,992,225 (GRCm38)	F141L	probably damaging	Het
Tia1	A	G	6: 86,420,348 (GRCm38)	D101G	probably damaging	Het
Ttc21a	T	A	9: 119,954,162 (GRCm38)		probably null	Het
Usp34	T	A	11: 23,460,651 (GRCm38)	N2700K	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,743,667 (GRCm38)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,744,775 (GRCm38)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,746,151 (GRCm38)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,747,998 (GRCm38)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,744,103 (GRCm38)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,744,077 (GRCm38)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,743,203 (GRCm38)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,742,965 (GRCm38)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,744,517 (GRCm38)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,744,969 (GRCm38)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,738,978 (GRCm38)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,738,968 (GRCm38)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,746,165 (GRCm38)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,743,159 (GRCm38)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,737,842 (GRCm38)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,746,775 (GRCm38)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,737,902 (GRCm38)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,744,557 (GRCm38)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,746,073 (GRCm38)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,743,212 (GRCm38)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,743,210 (GRCm38)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,736,921 (GRCm38)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,743,183 (GRCm38)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,746,777 (GRCm38)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,746,988 (GRCm38)	missense	probably benign
R8735:Mslnl	UTSW	17	25,745,088 (GRCm38)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,745,073 (GRCm38)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,742,720 (GRCm38)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,742,532 (GRCm38)	intron	probably benign
RF007:Mslnl	UTSW	17	25,743,228 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CTTTTCTACGAGTGAGTGCCCCTG -3'
(R):5'- GCAGTCACAATTTGTGCTGCCTC -3'

Sequencing Primer
(F):5'- TGCCCCTGCCATCAGAAG -3'
(R):5'- TCTGAAGCACTGAGCTGTAGG -3'

Posted On

2014-04-24