Mutagenetix > Incidental Mutation

Incidental Mutation 'R1640:Spen'

173444

Institutional Source

Beutler Lab

Gene Symbol

Spen

Ensembl Gene

ENSMUSG00000040761

Gene Name

spen family transcription repressor

Synonyms

Mint

MMRRC Submission

039676-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1640 (G1)

Quality Score

174

Status

Not validated

Chromosome

Chromosomal Location

141195201-141265908 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 141196254 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 3632 (I3632T)

Ref Sequence

ENSEMBL: ENSMUSP00000101412 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006377] [ENSMUST00000078886] [ENSMUST00000105786]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000006377

SMART Domains

Protein: ENSMUSP00000006377
Gene: ENSMUSG00000006215

Domain	Start	End	E-Value	Type
BTB	24	116	1.38e-27	SMART
low complexity region	203	222	N/A	INTRINSIC
ZnF_C2H2	297	319	6.42e-4	SMART
ZnF_C2H2	325	347	3.11e-2	SMART
ZnF_C2H2	353	375	2.49e-1	SMART
ZnF_C2H2	381	403	8.47e-4	SMART
ZnF_C2H2	409	431	8.47e-4	SMART
ZnF_C2H2	437	459	1.22e-4	SMART
ZnF_C2H2	465	487	4.94e-5	SMART
ZnF_C2H2	493	515	3.26e-5	SMART
ZnF_C2H2	521	543	7.26e-3	SMART
ZnF_C2H2	549	571	4.79e-3	SMART
ZnF_C2H2	577	599	1.58e-3	SMART
ZnF_C2H2	605	628	2.57e-3	SMART
low complexity region	654	674	N/A	INTRINSIC
ZnF_C2H2	708	730	4.4e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000078886
AA Change: I3609T

PolyPhen 2 Score 0.116 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000077925
Gene: ENSMUSG00000040761
AA Change: I3609T

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	617	632	N/A	INTRINSIC
low complexity region	669	691	N/A	INTRINSIC
low complexity region	695	720	N/A	INTRINSIC
low complexity region	749	773	N/A	INTRINSIC
coiled coil region	800	825	N/A	INTRINSIC
low complexity region	830	841	N/A	INTRINSIC
internal_repeat_2	844	954	6.27e-5	PROSPERO
coiled coil region	1494	1522	N/A	INTRINSIC
low complexity region	1587	1627	N/A	INTRINSIC
low complexity region	1635	1641	N/A	INTRINSIC
low complexity region	1642	1671	N/A	INTRINSIC
low complexity region	1747	1758	N/A	INTRINSIC
low complexity region	1810	1823	N/A	INTRINSIC
low complexity region	1888	1903	N/A	INTRINSIC
low complexity region	1940	1955	N/A	INTRINSIC
low complexity region	2003	2012	N/A	INTRINSIC
internal_repeat_2	2015	2115	6.27e-5	PROSPERO
low complexity region	2127	2147	N/A	INTRINSIC
low complexity region	2169	2191	N/A	INTRINSIC
low complexity region	2207	2219	N/A	INTRINSIC
low complexity region	2304	2323	N/A	INTRINSIC
low complexity region	2332	2371	N/A	INTRINSIC
low complexity region	2396	2413	N/A	INTRINSIC
low complexity region	2518	2533	N/A	INTRINSIC
low complexity region	2545	2555	N/A	INTRINSIC
low complexity region	2696	2722	N/A	INTRINSIC
low complexity region	2931	2942	N/A	INTRINSIC
low complexity region	2994	3006	N/A	INTRINSIC
low complexity region	3192	3212	N/A	INTRINSIC
low complexity region	3299	3337	N/A	INTRINSIC
Pfam:SPOC	3465	3586	2.7e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105786
AA Change: I3632T

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000101412
Gene: ENSMUSG00000040761
AA Change: I3632T

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	692	714	N/A	INTRINSIC
low complexity region	718	743	N/A	INTRINSIC
low complexity region	772	796	N/A	INTRINSIC
coiled coil region	823	848	N/A	INTRINSIC
low complexity region	853	864	N/A	INTRINSIC
internal_repeat_2	867	977	8.58e-5	PROSPERO
coiled coil region	1517	1545	N/A	INTRINSIC
low complexity region	1610	1650	N/A	INTRINSIC
low complexity region	1658	1664	N/A	INTRINSIC
low complexity region	1665	1694	N/A	INTRINSIC
low complexity region	1770	1781	N/A	INTRINSIC
low complexity region	1833	1846	N/A	INTRINSIC
low complexity region	1911	1926	N/A	INTRINSIC
low complexity region	1963	1978	N/A	INTRINSIC
low complexity region	2026	2035	N/A	INTRINSIC
internal_repeat_2	2038	2138	8.58e-5	PROSPERO
low complexity region	2150	2170	N/A	INTRINSIC
low complexity region	2192	2214	N/A	INTRINSIC
low complexity region	2230	2242	N/A	INTRINSIC
low complexity region	2327	2346	N/A	INTRINSIC
low complexity region	2355	2394	N/A	INTRINSIC
low complexity region	2419	2436	N/A	INTRINSIC
low complexity region	2541	2556	N/A	INTRINSIC
low complexity region	2568	2578	N/A	INTRINSIC
low complexity region	2719	2745	N/A	INTRINSIC
low complexity region	2954	2965	N/A	INTRINSIC
low complexity region	3017	3029	N/A	INTRINSIC
low complexity region	3215	3235	N/A	INTRINSIC
low complexity region	3322	3360	N/A	INTRINSIC
Pfam:SPOC	3488	3609	2.7e-29	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123477

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130482

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142020

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142438

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142695

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000147227

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.1%
20x: 92.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice die during late gestation exhibiting morphological abnormalities of the heart, pancreas, and liver. Inactivation of this gene also affects the differentiation of B cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810004N23Rik	C	A	8: 125,566,584 (GRCm39)	V279L	probably damaging	Het
Acot6	A	T	12: 84,147,900 (GRCm39)	Y52F	probably damaging	Het
Adam22	C	T	5: 8,195,689 (GRCm39)	V284I	probably damaging	Het
Agl	A	T	3: 116,545,739 (GRCm39)	H1352Q	probably benign	Het
Aldh18a1	G	A	19: 40,573,943 (GRCm39)	P27S	probably benign	Het
C1ra	A	G	6: 124,499,233 (GRCm39)	N473S	probably benign	Het
C87436	T	C	6: 86,423,233 (GRCm39)	L269P	probably damaging	Het
Calcrl	A	G	2: 84,164,021 (GRCm39)	V390A	probably damaging	Het
Ccp110	T	G	7: 118,314,751 (GRCm39)		probably null	Het
Cerk	A	G	15: 86,033,601 (GRCm39)	V274A	probably damaging	Het
Chd1l	A	T	3: 97,488,307 (GRCm39)	S570T	probably benign	Het
Chst14	T	A	2: 118,757,379 (GRCm39)	W83R	probably damaging	Het
Chsy1	A	T	7: 65,821,262 (GRCm39)	D499V	probably benign	Het
Ckmt1	C	A	2: 121,190,198 (GRCm39)		probably null	Het
Cnot1	A	T	8: 96,496,460 (GRCm39)	V282D	probably damaging	Het
Cntn3	A	T	6: 102,218,974 (GRCm39)	S549T	possibly damaging	Het
Cntnap5c	A	T	17: 58,702,289 (GRCm39)	D1203V	probably benign	Het
Col4a4	A	G	1: 82,513,491 (GRCm39)	Y169H	unknown	Het
Cwc22	A	G	2: 77,745,874 (GRCm39)	F454S	possibly damaging	Het
Dab1	C	T	4: 104,588,948 (GRCm39)	A524V	probably benign	Het
Dhrs7	A	T	12: 72,699,089 (GRCm39)	W298R	possibly damaging	Het
Dock7	G	T	4: 98,833,483 (GRCm39)	T1906N	probably damaging	Het
Dpy19l3	T	C	7: 35,449,203 (GRCm39)	T67A	probably benign	Het
Drc1	A	G	5: 30,521,301 (GRCm39)	D654G	possibly damaging	Het
Ech1	A	T	7: 28,531,264 (GRCm39)	H284L	probably damaging	Het
Epc1	G	A	18: 6,441,175 (GRCm39)	Q8*	probably null	Het
Etv5	A	T	16: 22,254,664 (GRCm39)	D65E	probably damaging	Het
F2rl3	A	G	8: 73,489,534 (GRCm39)	R254G	probably benign	Het
Fabp5	T	G	3: 10,080,170 (GRCm39)	F73L	probably benign	Het
Fbrs	T	C	7: 127,086,483 (GRCm39)	I611T	probably damaging	Het
Flnc	T	C	6: 29,433,806 (GRCm39)	S117P	possibly damaging	Het
Frmd4b	A	C	6: 97,285,634 (GRCm39)	S291A	possibly damaging	Het
Galnt13	A	G	2: 54,950,558 (GRCm39)	Y413C	probably damaging	Het
Gfer	A	G	17: 24,914,337 (GRCm39)	Y109H	possibly damaging	Het
Gli2	T	C	1: 118,764,254 (GRCm39)	H1299R	possibly damaging	Het
Gm10110	T	C	14: 90,135,679 (GRCm39)		noncoding transcript	Het
Gprc5a	T	G	6: 135,055,652 (GRCm39)	L33W	probably damaging	Het
Grin3a	T	C	4: 49,844,721 (GRCm39)	T121A	probably benign	Het
Grk3	A	G	5: 113,163,248 (GRCm39)	V33A	probably benign	Het
Hc	A	T	2: 34,947,336 (GRCm39)	Y59*	probably null	Het
Hrnr	A	G	3: 93,239,823 (GRCm39)	I3354V	unknown	Het
Hyou1	C	G	9: 44,300,703 (GRCm39)	T924S	probably benign	Het
Ifi209	T	G	1: 173,464,931 (GRCm39)	H20Q	probably damaging	Het
Ifi44	A	G	3: 151,438,171 (GRCm39)	V372A	probably benign	Het
Igdcc4	T	A	9: 65,030,077 (GRCm39)	L328H	probably damaging	Het
Jkampl	A	G	6: 73,445,869 (GRCm39)	Y227H	probably benign	Het
Kif18a	A	G	2: 109,120,161 (GRCm39)	T155A	probably benign	Het
Kmt2d	A	G	15: 98,742,938 (GRCm39)		probably benign	Het
Kri1	T	C	9: 21,191,753 (GRCm39)	D281G	possibly damaging	Het
Larp1b	T	A	3: 40,988,507 (GRCm39)	M1K	probably null	Het
Loxhd1	A	G	18: 77,490,259 (GRCm39)	D1225G	probably damaging	Het
Mzf1	A	G	7: 12,777,197 (GRCm39)	*736Q	probably null	Het
Naip2	C	T	13: 100,298,489 (GRCm39)	A516T	possibly damaging	Het
Ncf2	T	A	1: 152,683,784 (GRCm39)	M1K	probably null	Het
Or5t15	T	A	2: 86,681,571 (GRCm39)	H157L	probably benign	Het
Or8h10	A	C	2: 86,808,963 (GRCm39)	M59R	probably damaging	Het
Parp12	T	C	6: 39,073,574 (GRCm39)	D417G	probably benign	Het
Parp12	T	C	6: 39,088,612 (GRCm39)	H208R	probably damaging	Het
Pcif1	T	C	2: 164,727,603 (GRCm39)	I132T	probably benign	Het
Pck2	A	G	14: 55,786,041 (GRCm39)	D610G	possibly damaging	Het
Plbd1	T	C	6: 136,617,123 (GRCm39)	K185E	probably benign	Het
Ppp2r5a	T	C	1: 191,086,126 (GRCm39)	M425V	probably damaging	Het
Rapgef6	T	A	11: 54,548,231 (GRCm39)	V805D	probably damaging	Het
Rprd2	C	T	3: 95,671,059 (GRCm39)		probably benign	Het
Ryr3	A	G	2: 112,731,178 (GRCm39)	S711P	probably damaging	Het
Slc17a3	T	A	13: 24,036,340 (GRCm39)	L212*	probably null	Het
Slc4a8	A	G	15: 100,681,668 (GRCm39)	D41G	probably benign	Het
Slc9a3	T	A	13: 74,306,937 (GRCm39)	V354E	probably damaging	Het
Tesc	A	G	5: 118,192,914 (GRCm39)	S77G	probably benign	Het
Tet3	A	T	6: 83,346,297 (GRCm39)	V1245D	probably benign	Het
Tox4	T	A	14: 52,530,000 (GRCm39)	D553E	possibly damaging	Het
Tpmt	A	T	13: 47,180,759 (GRCm39)	Y193*	probably null	Het
Trio	T	C	15: 27,833,130 (GRCm39)	Y1169C	probably damaging	Het
Urb1	G	A	16: 90,569,514 (GRCm39)	T1404I	probably benign	Het
Usp47	T	C	7: 111,682,334 (GRCm39)	S540P	probably damaging	Het
Vmn1r178	A	T	7: 23,593,548 (GRCm39)	M126L	possibly damaging	Het
Vmn1r223	T	C	13: 23,434,348 (GRCm39)	F314S	probably damaging	Het
Vmn2r112	A	T	17: 22,824,097 (GRCm39)	I451L	probably benign	Het
Zfc3h1	A	G	10: 115,242,806 (GRCm39)		probably null	Het
Zfp503	A	T	14: 22,034,969 (GRCm39)	L649Q	probably damaging	Het
Zfp977	A	C	7: 42,229,530 (GRCm39)	C332G	probably damaging	Het

Other mutations in Spen

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Spen	APN	4	141,217,212 (GRCm39)	missense	unknown
IGL01357:Spen	APN	4	141,244,424 (GRCm39)	missense	unknown
IGL02184:Spen	APN	4	141,214,917 (GRCm39)	missense	unknown
IGL02226:Spen	APN	4	141,205,457 (GRCm39)	missense	unknown
IGL02321:Spen	APN	4	141,244,441 (GRCm39)	missense	unknown
IGL02350:Spen	APN	4	141,204,890 (GRCm39)	missense	unknown
IGL02357:Spen	APN	4	141,204,890 (GRCm39)	missense	unknown
IGL02627:Spen	APN	4	141,200,326 (GRCm39)	missense	probably damaging	0.99
IGL02683:Spen	APN	4	141,198,956 (GRCm39)	missense	probably benign	0.06
IGL02945:Spen	APN	4	141,221,624 (GRCm39)	missense	unknown
IGL02950:Spen	APN	4	141,196,819 (GRCm39)	missense	probably damaging	1.00
IGL03008:Spen	APN	4	141,203,448 (GRCm39)	missense	possibly damaging	0.70
IGL03019:Spen	APN	4	141,206,227 (GRCm39)	missense	unknown
IGL03038:Spen	APN	4	141,265,550 (GRCm39)	missense	unknown
IGL03334:Spen	APN	4	141,197,280 (GRCm39)	missense	probably damaging	1.00
filtered	UTSW	4	141,204,683 (GRCm39)	missense	unknown
mentholated	UTSW	4	141,196,711 (GRCm39)	missense	possibly damaging	0.78
R0105:Spen	UTSW	4	141,197,121 (GRCm39)	splice site	probably benign
R0268:Spen	UTSW	4	141,204,868 (GRCm39)	missense	unknown
R0359:Spen	UTSW	4	141,244,181 (GRCm39)	missense	unknown
R0394:Spen	UTSW	4	141,201,514 (GRCm39)	missense	probably benign	0.03
R0423:Spen	UTSW	4	141,206,647 (GRCm39)	missense	unknown
R0433:Spen	UTSW	4	141,211,069 (GRCm39)	missense	unknown
R0462:Spen	UTSW	4	141,200,962 (GRCm39)	missense	probably damaging	1.00
R0687:Spen	UTSW	4	141,215,339 (GRCm39)	missense	unknown
R0699:Spen	UTSW	4	141,201,702 (GRCm39)	missense	possibly damaging	0.72
R0865:Spen	UTSW	4	141,199,181 (GRCm39)	missense	probably benign	0.11
R0918:Spen	UTSW	4	141,212,875 (GRCm39)	missense	unknown
R1034:Spen	UTSW	4	141,203,063 (GRCm39)	missense	probably benign	0.33
R1341:Spen	UTSW	4	141,196,711 (GRCm39)	missense	possibly damaging	0.78
R1401:Spen	UTSW	4	141,199,132 (GRCm39)	missense	probably damaging	0.98
R1509:Spen	UTSW	4	141,202,946 (GRCm39)	missense	probably benign	0.00
R1509:Spen	UTSW	4	141,203,011 (GRCm39)	missense	possibly damaging	0.53
R1561:Spen	UTSW	4	141,199,694 (GRCm39)	nonsense	probably null
R1589:Spen	UTSW	4	141,215,335 (GRCm39)	missense	unknown
R1758:Spen	UTSW	4	141,203,686 (GRCm39)	missense	unknown
R1764:Spen	UTSW	4	141,200,261 (GRCm39)	missense	probably damaging	1.00
R1824:Spen	UTSW	4	141,200,096 (GRCm39)	missense	probably damaging	1.00
R1899:Spen	UTSW	4	141,197,654 (GRCm39)	missense	probably benign	0.17
R1916:Spen	UTSW	4	141,199,909 (GRCm39)	missense	probably damaging	1.00
R2011:Spen	UTSW	4	141,200,640 (GRCm39)	missense	probably damaging	1.00
R2295:Spen	UTSW	4	141,204,584 (GRCm39)	missense	unknown
R2379:Spen	UTSW	4	141,244,238 (GRCm39)	missense	unknown
R2404:Spen	UTSW	4	141,205,216 (GRCm39)	missense	unknown
R3719:Spen	UTSW	4	141,244,494 (GRCm39)	missense	unknown
R3889:Spen	UTSW	4	141,205,192 (GRCm39)	missense	unknown
R3945:Spen	UTSW	4	141,204,664 (GRCm39)	missense	unknown
R4227:Spen	UTSW	4	141,249,458 (GRCm39)	missense	unknown
R4326:Spen	UTSW	4	141,204,683 (GRCm39)	missense	unknown
R4382:Spen	UTSW	4	141,200,450 (GRCm39)	missense	possibly damaging	0.88
R4542:Spen	UTSW	4	141,204,097 (GRCm39)	missense	unknown
R4757:Spen	UTSW	4	141,200,390 (GRCm39)	nonsense	probably null
R4771:Spen	UTSW	4	141,199,907 (GRCm39)	missense	probably benign	0.14
R5072:Spen	UTSW	4	141,249,613 (GRCm39)	missense	unknown
R5121:Spen	UTSW	4	141,203,410 (GRCm39)	missense	probably benign	0.00
R5176:Spen	UTSW	4	141,203,587 (GRCm39)	missense	unknown
R5290:Spen	UTSW	4	141,201,127 (GRCm39)	missense	probably damaging	1.00
R5291:Spen	UTSW	4	141,215,390 (GRCm39)	missense	unknown
R5293:Spen	UTSW	4	141,199,717 (GRCm39)	missense	possibly damaging	0.89
R5347:Spen	UTSW	4	141,198,796 (GRCm39)	missense	probably benign	0.26
R5511:Spen	UTSW	4	141,244,149 (GRCm39)	missense	unknown
R5511:Spen	UTSW	4	141,202,375 (GRCm39)	missense	possibly damaging	0.86
R5772:Spen	UTSW	4	141,205,495 (GRCm39)	missense	unknown
R5834:Spen	UTSW	4	141,199,154 (GRCm39)	missense	possibly damaging	0.63
R5858:Spen	UTSW	4	141,201,182 (GRCm39)	missense	probably benign	0.05
R6214:Spen	UTSW	4	141,206,423 (GRCm39)	missense	unknown
R6232:Spen	UTSW	4	141,244,333 (GRCm39)	missense	unknown
R6345:Spen	UTSW	4	141,198,944 (GRCm39)	missense	possibly damaging	0.86
R6419:Spen	UTSW	4	141,203,621 (GRCm39)	missense	unknown
R6455:Spen	UTSW	4	141,202,820 (GRCm39)	missense	probably damaging	0.97
R6979:Spen	UTSW	4	141,205,374 (GRCm39)	missense	unknown
R6994:Spen	UTSW	4	141,220,770 (GRCm39)	missense	unknown
R7018:Spen	UTSW	4	141,220,755 (GRCm39)	missense	unknown
R7040:Spen	UTSW	4	141,221,693 (GRCm39)	missense	unknown
R7127:Spen	UTSW	4	141,203,419 (GRCm39)	missense	possibly damaging	0.53
R7218:Spen	UTSW	4	141,199,961 (GRCm39)	missense	possibly damaging	0.54
R7234:Spen	UTSW	4	141,206,446 (GRCm39)	missense	unknown
R7316:Spen	UTSW	4	141,204,365 (GRCm39)	missense	unknown
R7350:Spen	UTSW	4	141,206,696 (GRCm39)	missense	unknown
R7356:Spen	UTSW	4	141,199,235 (GRCm39)	nonsense	probably null
R7400:Spen	UTSW	4	141,201,052 (GRCm39)	missense	probably damaging	1.00
R7470:Spen	UTSW	4	141,206,605 (GRCm39)	missense	unknown
R7698:Spen	UTSW	4	141,200,156 (GRCm39)	missense	probably damaging	1.00
R7858:Spen	UTSW	4	141,215,442 (GRCm39)	splice site	probably null
R8033:Spen	UTSW	4	141,199,057 (GRCm39)	missense	probably benign	0.03
R8064:Spen	UTSW	4	141,203,011 (GRCm39)	missense	possibly damaging	0.53
R8159:Spen	UTSW	4	141,202,314 (GRCm39)	missense	possibly damaging	0.53
R8187:Spen	UTSW	4	141,200,216 (GRCm39)	missense	possibly damaging	0.93
R8463:Spen	UTSW	4	141,249,590 (GRCm39)	missense	unknown
R8557:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8558:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8672:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8673:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8674:Spen	UTSW	4	141,197,681 (GRCm39)	missense	probably benign	0.14
R8714:Spen	UTSW	4	141,215,314 (GRCm39)	missense	unknown
R8735:Spen	UTSW	4	141,197,129 (GRCm39)	missense	probably benign	0.32
R8762:Spen	UTSW	4	141,200,261 (GRCm39)	missense	probably damaging	1.00
R8877:Spen	UTSW	4	141,199,137 (GRCm39)	nonsense	probably null
R8878:Spen	UTSW	4	141,204,520 (GRCm39)	missense	unknown
R8937:Spen	UTSW	4	141,201,374 (GRCm39)	missense	probably damaging	1.00
R8939:Spen	UTSW	4	141,202,969 (GRCm39)	missense	possibly damaging	0.72
R8968:Spen	UTSW	4	141,197,701 (GRCm39)	missense	probably benign	0.02
R8971:Spen	UTSW	4	141,201,889 (GRCm39)	missense	possibly damaging	0.53
R9016:Spen	UTSW	4	141,200,938 (GRCm39)	missense	probably damaging	1.00
R9072:Spen	UTSW	4	141,203,702 (GRCm39)	missense	unknown
R9073:Spen	UTSW	4	141,203,702 (GRCm39)	missense	unknown
R9120:Spen	UTSW	4	141,200,233 (GRCm39)	missense
R9136:Spen	UTSW	4	141,249,623 (GRCm39)	missense	unknown
R9138:Spen	UTSW	4	141,196,797 (GRCm39)	missense	probably damaging	1.00
R9150:Spen	UTSW	4	141,244,468 (GRCm39)	missense	unknown
R9225:Spen	UTSW	4	141,202,943 (GRCm39)	missense	possibly damaging	0.53
R9492:Spen	UTSW	4	141,199,098 (GRCm39)	missense	probably benign	0.26
R9537:Spen	UTSW	4	141,244,156 (GRCm39)	small deletion	probably benign
R9537:Spen	UTSW	4	141,199,015 (GRCm39)	missense	probably benign	0.15
R9602:Spen	UTSW	4	141,205,183 (GRCm39)	missense	unknown
R9609:Spen	UTSW	4	141,215,419 (GRCm39)	missense	unknown
R9686:Spen	UTSW	4	141,199,946 (GRCm39)	missense	probably benign	0.27
R9697:Spen	UTSW	4	141,196,275 (GRCm39)	missense	probably damaging	1.00
R9713:Spen	UTSW	4	141,244,331 (GRCm39)	missense	unknown
T0722:Spen	UTSW	4	141,201,664 (GRCm39)	missense	probably benign	0.33
T0975:Spen	UTSW	4	141,201,664 (GRCm39)	missense	probably benign	0.33
Z1088:Spen	UTSW	4	141,205,287 (GRCm39)	missense	unknown
Z1088:Spen	UTSW	4	141,205,288 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ACACATGTCCAGAGCATCGCAG -3'
(R):5'- TGCCCATTCCAGAGAAAGGAGGTAG -3'

Sequencing Primer
(F):5'- CGCGAGTCACTAATTTAGTGC -3'
(R):5'- GACCTTGAACTATAGAACTGGTGAC -3'

Posted On

2014-04-24