Incidental Mutation 'R0103:Nanos3'
ID 17479
Institutional Source Beutler Lab
Gene Symbol Nanos3
Ensembl Gene ENSMUSG00000056155
Gene Name nanos C2HC-type zinc finger 3
Synonyms nos3
MMRRC Submission 038389-MU
Accession Numbers
Essential gene? Possibly essential (E-score: 0.731) question?
Stock # R0103 (G1)
Quality Score
Status Validated
Chromosome 8
Chromosomal Location 84900359-84911396 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to T at 84902763 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Glutamine at position 133 (R133Q)
Ref Sequence ENSEMBL: ENSMUSP00000147775 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000070102] [ENSMUST00000093375] [ENSMUST00000118856] [ENSMUST00000210202]
AlphaFold P60324
Predicted Effect probably damaging
Transcript: ENSMUST00000070102
AA Change: R133Q

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000067385
Gene: ENSMUSG00000056155
AA Change: R133Q

DomainStartEndE-ValueType
low complexity region 32 54 N/A INTRINSIC
Pfam:zf-nanos 57 110 1.2e-28 PFAM
low complexity region 157 178 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000083549
Predicted Effect probably benign
Transcript: ENSMUST00000093375
SMART Domains Protein: ENSMUSP00000091067
Gene: ENSMUSG00000008129

DomainStartEndE-ValueType
low complexity region 226 241 N/A INTRINSIC
low complexity region 291 306 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000102383
Predicted Effect probably benign
Transcript: ENSMUST00000118856
SMART Domains Protein: ENSMUSP00000113651
Gene: ENSMUSG00000008129

DomainStartEndE-ValueType
Pfam:DUF4671 1 193 2.1e-62 PFAM
Pfam:DUF4671 181 600 7.3e-131 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000210202
AA Change: R133Q

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000211482
Meta Mutation Damage Score 0.1597 question?
Coding Region Coverage
  • 1x: 89.4%
  • 3x: 86.4%
  • 10x: 77.8%
  • 20x: 63.0%
Validation Efficiency 91% (89/98)
MGI Phenotype PHENOTYPE: Homozygous inactivation of this locus results in both male and female infertility. Both male and female gonads are reduced in size and are devoid of germ cells, putatively due to impaired maintainence of primordial germ cells. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 A C 11: 9,223,951 (GRCm39) R443S probably damaging Het
Adgrl3 A G 5: 81,940,194 (GRCm39) probably benign Het
Anapc1 T C 2: 128,522,372 (GRCm39) probably benign Het
Aqr T A 2: 113,979,497 (GRCm39) I313F probably damaging Het
Arfgap3 A T 15: 83,206,922 (GRCm39) probably benign Het
Asah2 G T 19: 31,996,377 (GRCm39) H374N probably benign Het
Catspere2 A G 1: 177,943,771 (GRCm39) N703D unknown Het
Ccdc106 C A 7: 5,060,544 (GRCm39) Q35K probably benign Het
Ccm2l G T 2: 152,909,839 (GRCm39) E64* probably null Het
Cep85l A T 10: 53,154,270 (GRCm39) D776E possibly damaging Het
Cfap52 T A 11: 67,815,951 (GRCm39) I611F possibly damaging Het
Cldn22 C T 8: 48,277,589 (GRCm39) T9M probably benign Het
Coa7 T C 4: 108,195,338 (GRCm39) L89P possibly damaging Het
Cox7a2l A T 17: 83,821,701 (GRCm39) Y2N probably damaging Het
Cyp27a1 A C 1: 74,775,074 (GRCm39) E301A probably benign Het
Dennd4c A G 4: 86,730,683 (GRCm39) Y860C probably benign Het
Dhx58 T C 11: 100,586,096 (GRCm39) T642A probably damaging Het
Dlg4 A G 11: 69,922,019 (GRCm39) Y87C probably damaging Het
Dnah6 C T 6: 73,069,155 (GRCm39) E2511K probably damaging Het
Entpd5 C A 12: 84,443,717 (GRCm39) E9* probably null Het
Fbln2 A C 6: 91,248,532 (GRCm39) I1066L probably benign Het
Fhl2 C T 1: 43,192,381 (GRCm39) R4H probably benign Het
Frmpd1 T A 4: 45,229,884 (GRCm39) I17K probably damaging Het
Gbp7 T A 3: 142,252,299 (GRCm39) N627K probably benign Het
Gnptab A G 10: 88,265,381 (GRCm39) Y331C probably damaging Het
Hibadh T A 6: 52,534,862 (GRCm39) M173L probably benign Het
Itga1 T C 13: 115,152,790 (GRCm39) I211V probably benign Het
Keg1 A T 19: 12,696,280 (GRCm39) I155F possibly damaging Het
Ltb A G 17: 35,414,016 (GRCm39) probably benign Het
Manea A T 4: 26,329,080 (GRCm39) probably null Het
Ms4a4a T A 19: 11,370,048 (GRCm39) M202K possibly damaging Het
Myo3a T G 2: 22,436,360 (GRCm39) probably benign Het
Myo9b C T 8: 71,776,493 (GRCm39) probably benign Het
Ncor1 G T 11: 62,233,871 (GRCm39) Q444K possibly damaging Het
Nek7 A T 1: 138,471,980 (GRCm39) C53* probably null Het
Obscn G T 11: 58,953,522 (GRCm39) Y4044* probably null Het
Pcsk6 T C 7: 65,578,845 (GRCm39) probably benign Het
Phax T A 18: 56,695,785 (GRCm39) V7D probably benign Het
Phxr4 T C 9: 13,343,087 (GRCm39) probably benign Het
Pkhd1 T A 1: 20,593,583 (GRCm39) D1510V probably benign Het
Pkhd1l1 T C 15: 44,460,537 (GRCm39) C4249R probably benign Het
Prpf39 T C 12: 65,102,057 (GRCm39) V378A possibly damaging Het
Psd2 A G 18: 36,137,770 (GRCm39) N455S probably damaging Het
Ptpro T A 6: 137,420,592 (GRCm39) V1007D probably damaging Het
Rab4b A G 7: 26,873,927 (GRCm39) I117T probably benign Het
Rad9b A T 5: 122,469,590 (GRCm39) V348E probably damaging Het
Rcor1 T C 12: 111,076,212 (GRCm39) probably benign Het
Rhoc A T 3: 104,699,307 (GRCm39) E32V possibly damaging Het
Rnf40 T G 7: 127,199,743 (GRCm39) V925G probably damaging Het
Slc25a32 A T 15: 38,963,292 (GRCm39) Y176* probably null Het
Slc7a1 T A 5: 148,289,236 (GRCm39) K4* probably null Het
Taar2 G A 10: 23,817,393 (GRCm39) R311H probably benign Het
Taar4 A T 10: 23,837,304 (GRCm39) N305Y probably damaging Het
Tcaf1 G T 6: 42,663,324 (GRCm39) D185E probably benign Het
Tmem138 T C 19: 10,552,316 (GRCm39) N62S possibly damaging Het
Tnfrsf25 C T 4: 152,201,405 (GRCm39) P65S possibly damaging Het
Trp53bp1 A T 2: 121,067,240 (GRCm39) S495R possibly damaging Het
Trpv3 T C 11: 73,184,805 (GRCm39) F597S probably damaging Het
Ugt2a3 A G 5: 87,484,577 (GRCm39) V149A possibly damaging Het
Ush2a T G 1: 188,051,267 (GRCm39) I251R possibly damaging Het
Vamp4 T C 1: 162,417,108 (GRCm39) C114R possibly damaging Het
Zc3h13 T A 14: 75,567,908 (GRCm39) V1067E probably damaging Het
Zcwpw1 G A 5: 137,808,375 (GRCm39) W274* probably null Het
Other mutations in Nanos3
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0047:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0048:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0089:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0090:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0102:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0134:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0145:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0189:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0190:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0226:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0344:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R0494:Nanos3 UTSW 8 84,902,763 (GRCm39) missense probably damaging 1.00
R8971:Nanos3 UTSW 8 84,902,815 (GRCm39) missense probably benign 0.14
R9128:Nanos3 UTSW 8 84,903,080 (GRCm39) missense probably benign 0.00
Posted On 2013-01-31