Mutagenetix > Incidental Mutation

Incidental Mutation 'R1623:Itgb4'

174814

Institutional Source

Beutler Lab

Gene Symbol

Itgb4

Ensembl Gene

ENSMUSG00000020758

Gene Name

integrin beta 4

Synonyms

CD104

MMRRC Submission

039660-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1623 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

115865556-115899238 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 115882142 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 819 (Y819*)

Ref Sequence

ENSEMBL: ENSMUSP00000127604 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021107] [ENSMUST00000068981] [ENSMUST00000106458] [ENSMUST00000106460] [ENSMUST00000106461] [ENSMUST00000169928]

AlphaFold

A2A863

Predicted Effect

probably null
Transcript: ENSMUST00000021107
AA Change: Y819*

SMART Domains

Protein: ENSMUSP00000021107
Gene: ENSMUSG00000020758
AA Change: Y819*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000068981
AA Change: Y820*

SMART Domains

Protein: ENSMUSP00000070811
Gene: ENSMUSG00000020758
AA Change: Y820*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1459	1540	1.79e-12	SMART
FN3	1572	1656	1.7e-13	SMART
low complexity region	1689	1702	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106458
AA Change: Y820*

SMART Domains

Protein: ENSMUSP00000102066
Gene: ENSMUSG00000020758
AA Change: Y820*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106460
AA Change: Y820*

SMART Domains

Protein: ENSMUSP00000102068
Gene: ENSMUSG00000020758
AA Change: Y820*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1512	1593	1.79e-12	SMART
FN3	1625	1709	1.7e-13	SMART
low complexity region	1742	1755	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106461
AA Change: Y820*

SMART Domains

Protein: ENSMUSP00000102069
Gene: ENSMUSG00000020758
AA Change: Y820*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127523

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130286

Predicted Effect

probably null
Transcript: ENSMUST00000169928
AA Change: Y819*

SMART Domains

Protein: ENSMUSP00000127604
Gene: ENSMUSG00000020758
AA Change: Y819*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180782

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151691

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150129

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 94.9%
20x: 87.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations die shortly after birth with extensive detachment of the epidermis and other squamus epithelia. Stratified tissues lack hemidesmosomes and simple epithelia are also defective in adherence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acbd5	A	T	2: 22,984,356 (GRCm39)	D294V	probably damaging	Het
Actn2	A	T	13: 12,355,320 (GRCm39)	I21N	probably benign	Het
Adamts2	C	A	11: 50,558,942 (GRCm39)	P219H	possibly damaging	Het
Atg16l2	A	G	7: 100,939,113 (GRCm39)	F584L	probably benign	Het
Ccdc81	C	T	7: 89,535,390 (GRCm39)	R282Q	probably benign	Het
Ccn4	G	T	15: 66,763,448 (GRCm39)	V12L	possibly damaging	Het
Cd200r3	G	A	16: 44,771,811 (GRCm39)	C25Y	possibly damaging	Het
Cdkl4	C	T	17: 80,863,731 (GRCm39)		probably null	Het
Chrne	T	C	11: 70,509,254 (GRCm39)	E109G	possibly damaging	Het
Clmp	A	G	9: 40,693,856 (GRCm39)	T358A	probably benign	Het
Cmtr1	G	T	17: 29,906,021 (GRCm39)		probably null	Het
Col25a1	A	G	3: 130,343,699 (GRCm39)	E389G	probably damaging	Het
Ctc1	C	T	11: 68,911,968 (GRCm39)	T49M	probably damaging	Het
Cyth3	T	A	5: 143,687,127 (GRCm39)	M120K	probably damaging	Het
D430041D05Rik	T	A	2: 103,983,308 (GRCm39)	E1996V	probably damaging	Het
Dnah9	T	C	11: 65,928,463 (GRCm39)	M2069V	probably damaging	Het
Dsg1c	T	C	18: 20,408,234 (GRCm39)	Y428H	probably damaging	Het
Exosc8	T	A	3: 54,641,752 (GRCm39)	T7S	probably damaging	Het
F5	A	G	1: 164,023,191 (GRCm39)	Y1583C	probably damaging	Het
Fam178b	A	G	1: 36,683,405 (GRCm39)	I105T	probably damaging	Het
Fam181a	T	A	12: 103,282,591 (GRCm39)	Y165*	probably null	Het
Fbn2	G	T	18: 58,181,620 (GRCm39)	N1880K	possibly damaging	Het
Gbgt1	A	T	2: 28,394,988 (GRCm39)	M209L	probably benign	Het
Gkn2	C	A	6: 87,355,152 (GRCm39)	Y120*	probably null	Het
Gm14295	T	A	2: 176,499,157 (GRCm39)	D1E	probably damaging	Het
Gpr141	A	T	13: 19,936,082 (GRCm39)		probably null	Het
Gramd2b	C	A	18: 56,565,423 (GRCm39)	P26Q	probably benign	Het
Greb1	A	G	12: 16,724,771 (GRCm39)	I1801T	probably damaging	Het
Gstm3	T	A	3: 107,875,151 (GRCm39)	I64F	possibly damaging	Het
Gtse1	A	G	15: 85,751,779 (GRCm39)	Y324C	probably benign	Het
Hal	C	G	10: 93,352,159 (GRCm39)	T650R	probably benign	Het
Hdac7	G	A	15: 97,706,285 (GRCm39)	Q293*	probably null	Het
Hdlbp	A	T	1: 93,351,591 (GRCm39)	N437K	probably damaging	Het
Hif1an	A	G	19: 44,557,862 (GRCm39)	D248G	probably damaging	Het
Hivep3	T	C	4: 119,952,901 (GRCm39)	S406P	possibly damaging	Het
Hmcn2	A	T	2: 31,348,051 (GRCm39)	D4899V	possibly damaging	Het
Ikzf3	C	T	11: 98,381,157 (GRCm39)		probably null	Het
Inka2	G	A	3: 105,624,136 (GRCm39)	G151D	probably damaging	Het
Itpripl1	T	C	2: 126,983,555 (GRCm39)	D189G	possibly damaging	Het
Kmt2e	A	G	5: 23,687,500 (GRCm39)	Y450C	probably damaging	Het
Mc4r	A	G	18: 66,993,068 (GRCm39)	L15P	probably benign	Het
Mical1	T	G	10: 41,357,389 (GRCm39)		probably null	Het
Mical3	C	T	6: 121,001,768 (GRCm39)	V575M	probably damaging	Het
Mki67	A	T	7: 135,310,547 (GRCm39)		probably null	Het
Mocs2	G	A	13: 114,961,158 (GRCm39)	E52K	probably benign	Het
Myo7b	T	C	18: 32,133,104 (GRCm39)	N415S	probably damaging	Het
Notch1	G	A	2: 26,368,624 (GRCm39)	T555I	possibly damaging	Het
Notch3	T	C	17: 32,358,165 (GRCm39)	D1686G	probably benign	Het
Oit3	A	G	10: 59,264,061 (GRCm39)	F358L	probably damaging	Het
Or10x1	A	C	1: 174,196,515 (GRCm39)	I11L	probably benign	Het
Or6k4	A	T	1: 173,964,883 (GRCm39)	D191V	probably damaging	Het
Orai1	T	A	5: 123,167,265 (GRCm39)	I146N	probably damaging	Het
Pck1	A	G	2: 172,996,511 (GRCm39)	I142V	probably benign	Het
Pdap1	C	T	5: 145,069,739 (GRCm39)	V89M	possibly damaging	Het
Phf11a	T	C	14: 59,525,000 (GRCm39)	D68G	possibly damaging	Het
Pilrb2	T	C	5: 137,869,510 (GRCm39)	N30S	probably damaging	Het
Pkd1	G	A	17: 24,797,243 (GRCm39)	V2551I	probably damaging	Het
Pnpla7	T	C	2: 24,942,611 (GRCm39)	V132A	probably damaging	Het
Prr35	G	A	17: 26,166,508 (GRCm39)	P343L	probably benign	Het
Rad18	C	T	6: 112,605,480 (GRCm39)	S398N	probably damaging	Het
Rdh16f1	C	A	10: 127,626,722 (GRCm39)	N258K	probably benign	Het
Riox2	A	G	16: 59,303,405 (GRCm39)	H240R	probably damaging	Het
Ruvbl1	C	T	6: 88,462,752 (GRCm39)	A292V	probably damaging	Het
Ryr1	C	T	7: 28,794,915 (GRCm39)	G1151S	probably damaging	Het
Serpinb9b	T	C	13: 33,213,548 (GRCm39)	I35T	possibly damaging	Het
Slc1a1	T	A	19: 28,882,122 (GRCm39)	M328K	probably benign	Het
Slc7a13	A	T	4: 19,824,031 (GRCm39)	T267S	possibly damaging	Het
Speer3	G	T	5: 13,846,335 (GRCm39)	M218I	probably benign	Het
Sptan1	A	G	2: 29,876,432 (GRCm39)	I271V	probably damaging	Het
Swap70	A	G	7: 109,863,255 (GRCm39)	T195A	probably benign	Het
Tgs1	T	A	4: 3,585,964 (GRCm39)	N280K	probably benign	Het
Tonsl	A	G	15: 76,522,709 (GRCm39)	C181R	probably damaging	Het
Trdn	A	G	10: 33,134,098 (GRCm39)	K333R	possibly damaging	Het
Trpc4	T	A	3: 54,206,600 (GRCm39)	M600K	probably damaging	Het
Ubr3	C	T	2: 69,808,067 (GRCm39)	Q1183*	probably null	Het
Ubxn4	T	A	1: 128,200,588 (GRCm39)	L360I	possibly damaging	Het
Ugt2b1	T	A	5: 87,074,267 (GRCm39)	T31S	probably benign	Het
Uspl1	T	C	5: 149,152,009 (GRCm39)	S1056P	probably damaging	Het
Vmn2r60	A	T	7: 41,785,279 (GRCm39)	K164*	probably null	Het
Vwce	T	A	19: 10,624,108 (GRCm39)	L333*	probably null	Het
Wdr95	T	A	5: 149,497,581 (GRCm39)	L253Q	probably damaging	Het
Zfp113	T	C	5: 138,143,930 (GRCm39)	M107V	probably benign	Het
Zfp142	T	C	1: 74,610,934 (GRCm39)	T851A	possibly damaging	Het

Other mutations in Itgb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Itgb4	APN	11	115,881,766 (GRCm39)	missense	probably damaging	1.00
IGL01391:Itgb4	APN	11	115,881,746 (GRCm39)	missense	probably damaging	1.00
IGL01431:Itgb4	APN	11	115,897,283 (GRCm39)	splice site	probably benign
IGL01750:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01752:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01756:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01766:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01769:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02188:Itgb4	APN	11	115,894,213 (GRCm39)	missense	probably benign	0.08
IGL02262:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02293:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02318:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02319:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02338:Itgb4	APN	11	115,898,795 (GRCm39)	missense	probably damaging	1.00
IGL02734:Itgb4	APN	11	115,896,792 (GRCm39)	missense	probably benign
IGL02879:Itgb4	APN	11	115,885,178 (GRCm39)	missense	probably benign	0.05
IGL02889:Itgb4	APN	11	115,879,731 (GRCm39)	missense	probably damaging	1.00
IGL03183:Itgb4	APN	11	115,879,550 (GRCm39)	missense	probably damaging	1.00
IGL03054:Itgb4	UTSW	11	115,891,166 (GRCm39)	nonsense	probably null
R0021:Itgb4	UTSW	11	115,870,453 (GRCm39)	missense	possibly damaging	0.95
R0092:Itgb4	UTSW	11	115,869,950 (GRCm39)	missense	probably damaging	1.00
R0305:Itgb4	UTSW	11	115,870,238 (GRCm39)	missense	probably damaging	1.00
R0408:Itgb4	UTSW	11	115,898,428 (GRCm39)	missense	probably damaging	0.99
R0465:Itgb4	UTSW	11	115,870,582 (GRCm39)	missense	probably damaging	1.00
R0499:Itgb4	UTSW	11	115,870,521 (GRCm39)	missense	probably benign	0.00
R0535:Itgb4	UTSW	11	115,881,835 (GRCm39)	missense	possibly damaging	0.86
R0571:Itgb4	UTSW	11	115,870,594 (GRCm39)	missense	possibly damaging	0.94
R0613:Itgb4	UTSW	11	115,884,168 (GRCm39)	missense	probably damaging	0.98
R0838:Itgb4	UTSW	11	115,888,988 (GRCm39)	intron	probably benign
R1381:Itgb4	UTSW	11	115,885,163 (GRCm39)	missense	probably benign	0.00
R1451:Itgb4	UTSW	11	115,881,710 (GRCm39)	missense	probably damaging	1.00
R1459:Itgb4	UTSW	11	115,869,937 (GRCm39)	missense	probably benign	0.42
R1460:Itgb4	UTSW	11	115,874,990 (GRCm39)	missense	probably damaging	0.96
R1473:Itgb4	UTSW	11	115,874,873 (GRCm39)	missense	probably benign	0.01
R1484:Itgb4	UTSW	11	115,890,625 (GRCm39)	missense	probably benign	0.01
R1593:Itgb4	UTSW	11	115,871,817 (GRCm39)	missense	probably damaging	1.00
R1633:Itgb4	UTSW	11	115,898,586 (GRCm39)	missense	probably damaging	1.00
R1642:Itgb4	UTSW	11	115,898,183 (GRCm39)	missense	probably damaging	1.00
R1669:Itgb4	UTSW	11	115,882,156 (GRCm39)	missense	probably benign	0.07
R1713:Itgb4	UTSW	11	115,894,315 (GRCm39)	missense	probably damaging	1.00
R1732:Itgb4	UTSW	11	115,879,744 (GRCm39)	missense	probably damaging	1.00
R1791:Itgb4	UTSW	11	115,879,346 (GRCm39)	missense	probably damaging	1.00
R1847:Itgb4	UTSW	11	115,874,590 (GRCm39)	missense	probably benign	0.31
R1902:Itgb4	UTSW	11	115,871,564 (GRCm39)	missense	probably damaging	0.98
R1945:Itgb4	UTSW	11	115,884,279 (GRCm39)	nonsense	probably null
R2102:Itgb4	UTSW	11	115,896,561 (GRCm39)	missense	probably benign	0.23
R2184:Itgb4	UTSW	11	115,870,450 (GRCm39)	missense	probably damaging	0.96
R2334:Itgb4	UTSW	11	115,884,261 (GRCm39)	missense	probably damaging	1.00
R2401:Itgb4	UTSW	11	115,897,389 (GRCm39)	missense	possibly damaging	0.67
R3743:Itgb4	UTSW	11	115,894,496 (GRCm39)	missense	probably damaging	1.00
R3938:Itgb4	UTSW	11	115,896,752 (GRCm39)	missense	possibly damaging	0.92
R4134:Itgb4	UTSW	11	115,897,296 (GRCm39)	missense	probably benign	0.03
R4280:Itgb4	UTSW	11	115,881,761 (GRCm39)	missense	probably damaging	1.00
R4342:Itgb4	UTSW	11	115,879,555 (GRCm39)	missense	probably benign	0.01
R4434:Itgb4	UTSW	11	115,890,640 (GRCm39)	missense	probably benign	0.10
R4505:Itgb4	UTSW	11	115,874,087 (GRCm39)	splice site	silent
R4585:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4586:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4601:Itgb4	UTSW	11	115,896,548 (GRCm39)	missense	probably damaging	1.00
R4921:Itgb4	UTSW	11	115,897,431 (GRCm39)	missense	probably benign	0.12
R4962:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5027:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5029:Itgb4	UTSW	11	115,879,417 (GRCm39)	intron	probably benign
R5084:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5085:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5124:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5125:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5150:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5175:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5176:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5179:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5207:Itgb4	UTSW	11	115,897,365 (GRCm39)	missense	probably damaging	1.00
R5263:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5264:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5334:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5337:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5344:Itgb4	UTSW	11	115,880,575 (GRCm39)	missense	probably null	0.92
R5391:Itgb4	UTSW	11	115,875,894 (GRCm39)	missense	probably benign	0.05
R5437:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5440:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5653:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5654:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5655:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5772:Itgb4	UTSW	11	115,879,258 (GRCm39)	intron	probably benign
R5812:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5813:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5814:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5863:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5864:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5865:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5951:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5954:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5982:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6043:Itgb4	UTSW	11	115,870,212 (GRCm39)	missense	probably benign	0.30
R6133:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6134:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6135:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6169:Itgb4	UTSW	11	115,885,102 (GRCm39)	missense	probably damaging	0.98
R6172:Itgb4	UTSW	11	115,891,237 (GRCm39)	missense	probably benign	0.23
R6255:Itgb4	UTSW	11	115,888,963 (GRCm39)	missense	possibly damaging	0.83
R6258:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6259:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6260:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6612:Itgb4	UTSW	11	115,874,897 (GRCm39)	missense	probably benign	0.00
R7037:Itgb4	UTSW	11	115,896,391 (GRCm39)	nonsense	probably null
R7371:Itgb4	UTSW	11	115,888,906 (GRCm39)	missense	probably benign	0.29
R7605:Itgb4	UTSW	11	115,897,302 (GRCm39)	missense	probably benign	0.01
R7659:Itgb4	UTSW	11	115,870,557 (GRCm39)	missense	probably damaging	1.00
R7759:Itgb4	UTSW	11	115,894,536 (GRCm39)	missense	possibly damaging	0.92
R7804:Itgb4	UTSW	11	115,894,510 (GRCm39)	missense	probably damaging	1.00
R7832:Itgb4	UTSW	11	115,891,087 (GRCm39)	missense	probably damaging	1.00
R7842:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.18
R7923:Itgb4	UTSW	11	115,873,525 (GRCm39)	critical splice acceptor site	probably null
R8004:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.00
R8143:Itgb4	UTSW	11	115,884,255 (GRCm39)	missense	probably damaging	1.00
R8427:Itgb4	UTSW	11	115,882,544 (GRCm39)	critical splice donor site	probably null
R8857:Itgb4	UTSW	11	115,871,853 (GRCm39)	missense	probably benign	0.04
R8863:Itgb4	UTSW	11	115,875,898 (GRCm39)	nonsense	probably null
R8932:Itgb4	UTSW	11	115,879,295 (GRCm39)	missense	probably benign	0.01
R9153:Itgb4	UTSW	11	115,874,879 (GRCm39)	missense	probably benign	0.00
R9207:Itgb4	UTSW	11	115,897,923 (GRCm39)	missense	probably damaging	1.00
R9239:Itgb4	UTSW	11	115,898,130 (GRCm39)	missense	probably damaging	1.00
R9267:Itgb4	UTSW	11	115,870,465 (GRCm39)	missense	probably benign
R9289:Itgb4	UTSW	11	115,885,187 (GRCm39)	missense	probably benign	0.01
R9328:Itgb4	UTSW	11	115,880,625 (GRCm39)	missense	probably benign	0.00
R9435:Itgb4	UTSW	11	115,895,855 (GRCm39)	missense	probably benign	0.01
R9450:Itgb4	UTSW	11	115,874,097 (GRCm39)	missense	probably damaging	1.00
R9649:Itgb4	UTSW	11	115,885,171 (GRCm39)	missense	possibly damaging	0.78
R9779:Itgb4	UTSW	11	115,882,485 (GRCm39)	missense	probably damaging	1.00
X0062:Itgb4	UTSW	11	115,884,278 (GRCm39)	missense	probably damaging	1.00
Z1176:Itgb4	UTSW	11	115,897,346 (GRCm39)	missense	probably damaging	1.00
Z1177:Itgb4	UTSW	11	115,888,884 (GRCm39)	missense	probably benign	0.00
Z1177:Itgb4	UTSW	11	115,877,637 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GTCCCATCTTGATGGCACCAGAAC -3'
(R):5'- TGCCTGAGGGATGAACCCTGAAAC -3'

Sequencing Primer
(F):5'- TCTCTGTAGTGCCTTCTGCA -3'
(R):5'- TTCGAAGCACATGTCCAAAGAG -3'

Posted On

2014-04-24