Incidental Mutation 'R1568:Rgs20'
ID 175387
Institutional Source Beutler Lab
Gene Symbol Rgs20
Ensembl Gene ENSMUSG00000002459
Gene Name regulator of G-protein signaling 20
Synonyms Rgsz1, 2900073E09Rik
MMRRC Submission 039607-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R1568 (G1)
Quality Score 225
Status Not validated
Chromosome 1
Chromosomal Location 4979799-5140508 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 5091050 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Serine at position 127 (R127S)
Ref Sequence ENSEMBL: ENSMUSP00000113398 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000002533] [ENSMUST00000118000] [ENSMUST00000119256] [ENSMUST00000147158] [ENSMUST00000170566]
AlphaFold Q9QZB1
Predicted Effect probably benign
Transcript: ENSMUST00000002533
SMART Domains Protein: ENSMUSP00000002533
Gene: ENSMUSG00000002459

low complexity region 8 19 N/A INTRINSIC
low complexity region 59 71 N/A INTRINSIC
RGS 113 229 2.09e-48 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000118000
AA Change: R127S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000113398
Gene: ENSMUSG00000002459
AA Change: R127S

low complexity region 123 135 N/A INTRINSIC
low complexity region 150 161 N/A INTRINSIC
low complexity region 192 204 N/A INTRINSIC
RGS 246 362 2.09e-48 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000119256
SMART Domains Protein: ENSMUSP00000113324
Gene: ENSMUSG00000002459

low complexity region 28 40 N/A INTRINSIC
RGS 82 198 2.09e-48 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000147158
SMART Domains Protein: ENSMUSP00000117380
Gene: ENSMUSG00000002459

low complexity region 92 104 N/A INTRINSIC
Pfam:RGS 146 200 2.8e-16 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000156289
Predicted Effect probably benign
Transcript: ENSMUST00000170566
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.2%
  • 10x: 95.8%
  • 20x: 90.9%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam18 T G 8: 25,137,799 (GRCm39) probably null Het
Adgb A T 10: 10,318,409 (GRCm39) Y138* probably null Het
Adra1d T C 2: 131,388,092 (GRCm39) R488G possibly damaging Het
Ahnak G A 19: 8,979,739 (GRCm39) G341E probably damaging Het
Ankmy1 T C 1: 92,808,838 (GRCm39) D690G probably damaging Het
Arhgef38 C T 3: 132,838,225 (GRCm39) E21K probably damaging Het
Atp8b2 T C 3: 89,857,155 (GRCm39) M402V probably damaging Het
Atp8b4 G T 2: 126,167,314 (GRCm39) H1062N probably benign Het
Bdnf A G 2: 109,554,139 (GRCm39) H131R probably damaging Het
Cblb C T 16: 51,956,192 (GRCm39) T265M probably damaging Het
Cenpe T A 3: 134,945,519 (GRCm39) M1011K probably benign Het
Clca3a2 A T 3: 144,781,410 (GRCm39) Y711* probably null Het
Clca4a A G 3: 144,658,690 (GRCm39) Y842H probably benign Het
Clspn T A 4: 126,475,310 (GRCm39) M1021K probably benign Het
Cpne8 G T 15: 90,503,845 (GRCm39) R107S probably damaging Het
D6Ertd527e C G 6: 87,088,506 (GRCm39) T223S unknown Het
Dna2 C T 10: 62,784,966 (GRCm39) R28W probably benign Het
Dnah5 T A 15: 28,409,323 (GRCm39) N3580K probably damaging Het
Dsp T C 13: 38,359,123 (GRCm39) I298T probably damaging Het
Dync2h1 T C 9: 7,157,553 (GRCm39) K858R probably null Het
Fasn A T 11: 120,704,075 (GRCm39) V1448E possibly damaging Het
Ghdc A G 11: 100,659,331 (GRCm39) I322T probably benign Het
Gins2 T C 8: 121,308,939 (GRCm39) D105G probably damaging Het
Insr A T 8: 3,215,576 (GRCm39) D975E probably benign Het
Klrc3 T C 6: 129,616,510 (GRCm39) D169G probably benign Het
Krt76 T A 15: 101,793,443 (GRCm39) S532C unknown Het
Lamp3 A T 16: 19,492,275 (GRCm39) M323K probably damaging Het
Lgmn T C 12: 102,360,868 (GRCm39) I423M possibly damaging Het
Lpgat1 A T 1: 191,508,538 (GRCm39) T359S possibly damaging Het
Lrguk T A 6: 34,063,373 (GRCm39) I466N probably damaging Het
Magi3 A C 3: 103,996,843 (GRCm39) M234R probably benign Het
Myh14 G A 7: 44,261,122 (GRCm39) R1042* probably null Het
Nfia T G 4: 97,999,461 (GRCm39) Y378D possibly damaging Het
Npffr1 T A 10: 61,462,012 (GRCm39) S383T possibly damaging Het
Or4f57 C A 2: 111,790,585 (GRCm39) V278F probably benign Het
Or52n4 T A 7: 104,293,977 (GRCm39) I199F probably benign Het
Or5b112 C A 19: 13,319,539 (GRCm39) P139Q probably benign Het
Or9s14 T C 1: 92,535,676 (GRCm39) L39P probably damaging Het
Osr1 G A 12: 9,629,798 (GRCm39) probably null Het
Pde4b A C 4: 102,454,896 (GRCm39) R375S probably damaging Het
Pde8a A G 7: 80,942,011 (GRCm39) E150G probably damaging Het
Pkhd1l1 A T 15: 44,408,897 (GRCm39) probably null Het
Ppp3ca A G 3: 136,634,305 (GRCm39) T422A probably benign Het
Proser1 T C 3: 53,385,180 (GRCm39) V354A possibly damaging Het
Sanbr T C 11: 23,539,971 (GRCm39) K48E probably damaging Het
Sec22a T C 16: 35,167,998 (GRCm39) D171G probably benign Het
Secisbp2 A G 13: 51,827,143 (GRCm39) E417G possibly damaging Het
Serpinb6b T C 13: 33,158,895 (GRCm39) L32S probably damaging Het
Sf3b1 C G 1: 55,058,554 (GRCm39) E12Q possibly damaging Het
Slc39a10 A T 1: 46,865,375 (GRCm39) S487T probably benign Het
Slc6a1 G T 6: 114,284,731 (GRCm39) G263V probably damaging Het
Spag6 A G 2: 18,737,925 (GRCm39) D265G probably benign Het
Spata31e2 T C 1: 26,724,950 (GRCm39) T77A probably benign Het
Sybu G T 15: 44,582,228 (GRCm39) S132* probably null Het
Virma T C 4: 11,528,776 (GRCm39) S1288P probably damaging Het
Vmn1r229 T A 17: 21,035,067 (GRCm39) V104D probably damaging Het
Vmn1r238 T C 18: 3,123,358 (GRCm39) T19A probably benign Het
Vmn2r86 C T 10: 130,289,010 (GRCm39) V164I probably benign Het
Wfdc13 G T 2: 164,528,854 (GRCm39) A64S probably damaging Het
Zfp873 C A 10: 81,896,113 (GRCm39) H318Q probably damaging Het
Zyg11a A G 4: 108,040,843 (GRCm39) probably null Het
Other mutations in Rgs20
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00840:Rgs20 APN 1 5,140,238 (GRCm39) missense probably benign 0.04
IGL01433:Rgs20 APN 1 5,140,300 (GRCm39) missense possibly damaging 0.85
IGL01691:Rgs20 APN 1 4,987,145 (GRCm39) missense probably benign 0.00
R0573:Rgs20 UTSW 1 5,091,037 (GRCm39) missense possibly damaging 0.85
R1274:Rgs20 UTSW 1 4,982,670 (GRCm39) missense probably damaging 1.00
R1305:Rgs20 UTSW 1 5,091,262 (GRCm39) splice site probably null
R1513:Rgs20 UTSW 1 4,982,560 (GRCm39) missense probably damaging 1.00
R1763:Rgs20 UTSW 1 4,980,863 (GRCm39) missense probably damaging 1.00
R1794:Rgs20 UTSW 1 4,980,795 (GRCm39) missense probably damaging 1.00
R2118:Rgs20 UTSW 1 4,987,113 (GRCm39) splice site probably benign
R2437:Rgs20 UTSW 1 5,140,370 (GRCm39) splice site probably null
R3029:Rgs20 UTSW 1 5,140,276 (GRCm39) missense probably benign 0.00
R4665:Rgs20 UTSW 1 5,091,231 (GRCm39) missense probably benign 0.00
R5014:Rgs20 UTSW 1 4,980,770 (GRCm39) missense probably damaging 1.00
R5433:Rgs20 UTSW 1 5,140,333 (GRCm39) missense possibly damaging 0.93
R5620:Rgs20 UTSW 1 4,982,666 (GRCm39) missense probably damaging 1.00
R5880:Rgs20 UTSW 1 4,994,104 (GRCm39) missense probably damaging 0.99
R5990:Rgs20 UTSW 1 4,982,553 (GRCm39) missense probably benign 0.11
R6188:Rgs20 UTSW 1 5,091,106 (GRCm39) frame shift probably null
R7323:Rgs20 UTSW 1 4,982,535 (GRCm39) critical splice donor site probably null
R7459:Rgs20 UTSW 1 4,980,857 (GRCm39) missense probably benign 0.04
R7467:Rgs20 UTSW 1 4,982,553 (GRCm39) missense probably benign 0.11
R8101:Rgs20 UTSW 1 4,982,638 (GRCm39) missense probably benign 0.16
R9087:Rgs20 UTSW 1 4,994,190 (GRCm39) missense possibly damaging 0.88
R9193:Rgs20 UTSW 1 5,091,067 (GRCm39) missense possibly damaging 0.65
R9443:Rgs20 UTSW 1 4,982,629 (GRCm39) nonsense probably null
R9725:Rgs20 UTSW 1 4,980,793 (GRCm39) nonsense probably null
Z1176:Rgs20 UTSW 1 5,140,337 (GRCm39) missense probably benign
Z1177:Rgs20 UTSW 1 5,091,242 (GRCm39) frame shift probably null
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2014-04-24