Mutagenetix > Incidental Mutation

Incidental Mutation 'R1591:Mdn1'

175470

Institutional Source

Beutler Lab

Gene Symbol

Mdn1

Ensembl Gene

ENSMUSG00000058006

Gene Name

midasin AAA ATPase 1

Synonyms

4833432B22Rik, D4Abb1e, LOC213784

MMRRC Submission

039628-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1591 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32657119-32775217 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 32700092 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 1395 (V1395A)

Ref Sequence

ENSEMBL: ENSMUSP00000136222 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071642] [ENSMUST00000178134]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000071642
AA Change: V1395A

PolyPhen 2 Score 0.293 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000071569
Gene: ENSMUSG00000058006
AA Change: V1395A

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	7.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4672	4681	N/A	INTRINSIC
low complexity region	4735	4756	N/A	INTRINSIC
low complexity region	4769	4790	N/A	INTRINSIC
low complexity region	4886	4905	N/A	INTRINSIC
low complexity region	4924	4937	N/A	INTRINSIC
coiled coil region	4957	4983	N/A	INTRINSIC
low complexity region	5000	5017	N/A	INTRINSIC
low complexity region	5176	5192	N/A	INTRINSIC
low complexity region	5315	5329	N/A	INTRINSIC
VWA	5375	5556	2.73e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125323

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136608

Predicted Effect

possibly damaging
Transcript: ENSMUST00000178134
AA Change: V1395A

PolyPhen 2 Score 0.650 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000136222
Gene: ENSMUSG00000058006
AA Change: V1395A

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	4.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4667	4676	N/A	INTRINSIC
low complexity region	4730	4751	N/A	INTRINSIC
low complexity region	4764	4785	N/A	INTRINSIC
low complexity region	4881	4900	N/A	INTRINSIC
low complexity region	4919	4932	N/A	INTRINSIC
coiled coil region	4952	4978	N/A	INTRINSIC
low complexity region	4992	5010	N/A	INTRINSIC
low complexity region	5169	5185	N/A	INTRINSIC
low complexity region	5308	5322	N/A	INTRINSIC
VWA	5368	5549	2.73e-6	SMART

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.5%
20x: 89.7%

Validation Efficiency

100% (80/80)

Allele List at MGI

All alleles(29) : Targeted, other(2) Gene trapped(27)

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abraxas1	G	T	5: 100,957,505 (GRCm39)	Q201K	probably benign	Het
Acacb	A	T	5: 114,341,484 (GRCm39)	I829F	possibly damaging	Het
Acvr1b	G	T	15: 101,091,905 (GRCm39)	V62L	probably benign	Het
Adam6b	A	T	12: 113,453,452 (GRCm39)	I90F	probably benign	Het
Adgrf1	T	C	17: 43,621,872 (GRCm39)	L703P	probably damaging	Het
Arf3	T	C	15: 98,640,669 (GRCm39)		probably benign	Het
Asns	T	C	6: 7,678,007 (GRCm39)	D357G	probably damaging	Het
Atr	T	C	9: 95,827,438 (GRCm39)	M2461T	probably damaging	Het
B3galt4	G	A	17: 34,169,813 (GRCm39)	R142C	probably damaging	Het
Bod1l	T	C	5: 41,976,563 (GRCm39)	I1584V	probably benign	Het
Bub3	T	C	7: 131,163,337 (GRCm39)		probably null	Het
Camkk2	A	G	5: 122,895,621 (GRCm39)		probably null	Het
Cand1	G	T	10: 119,047,774 (GRCm39)	T572K	possibly damaging	Het
Caprin2	A	T	6: 148,774,606 (GRCm39)	S235R	possibly damaging	Het
Cdh24	T	C	14: 54,873,799 (GRCm39)	T452A	probably benign	Het
Cdh4	T	C	2: 179,528,657 (GRCm39)		probably null	Het
Cfap61	A	G	2: 145,987,378 (GRCm39)	R1060G	probably benign	Het
Chd9	A	G	8: 91,710,166 (GRCm39)	D314G	probably damaging	Het
Cplx3	C	T	9: 57,523,085 (GRCm39)	V125I	probably benign	Het
Csmd1	T	A	8: 15,950,710 (GRCm39)	I3500F	probably damaging	Het
Dnah1	G	T	14: 30,994,289 (GRCm39)	Q2858K	probably benign	Het
Dnah6	C	T	6: 73,053,583 (GRCm39)	E2884K	probably benign	Het
Ecm1	G	A	3: 95,643,275 (GRCm39)	R342C	probably damaging	Het
Exosc10	T	A	4: 148,652,840 (GRCm39)	I485N	probably benign	Het
Fap	T	C	2: 62,384,201 (GRCm39)	K2E	probably damaging	Het
Fgfr1	G	A	8: 26,062,736 (GRCm39)	G671D	probably damaging	Het
Fzr1	C	T	10: 81,206,201 (GRCm39)	R162Q	possibly damaging	Het
Grap2	A	T	15: 80,532,649 (GRCm39)	Y272F	probably damaging	Het
Il11ra1	T	A	4: 41,766,200 (GRCm39)	W246R	probably damaging	Het
Kcna3	A	T	3: 106,944,345 (GRCm39)	I203F	probably damaging	Het
Kcng1	T	C	2: 168,110,630 (GRCm39)	E178G	possibly damaging	Het
Kif19a	A	C	11: 114,680,057 (GRCm39)	H798P	probably benign	Het
Kif21b	T	C	1: 136,077,055 (GRCm39)	L359P	probably damaging	Het
Krt6a	A	T	15: 101,600,792 (GRCm39)		probably null	Het
Lrp1	C	T	10: 127,441,475 (GRCm39)	S216N	probably benign	Het
Lrp3	A	G	7: 34,901,790 (GRCm39)	V676A	probably benign	Het
Lrrc63	T	A	14: 75,363,332 (GRCm39)	K266N	possibly damaging	Het
Mccc1	C	T	3: 36,044,006 (GRCm39)	V246M	probably damaging	Het
Mmrn1	A	T	6: 60,921,755 (GRCm39)	R71*	probably null	Het
Mrps2	C	T	2: 28,359,500 (GRCm39)	A119V	probably benign	Het
Ogdh	T	C	11: 6,299,384 (GRCm39)	F750S	probably damaging	Het
Or1ak2	C	A	2: 36,827,990 (GRCm39)	N286K	probably damaging	Het
Or51aa2	T	G	7: 103,188,274 (GRCm39)	T56P	probably benign	Het
Or5p60	T	A	7: 107,723,571 (GRCm39)	I300F	possibly damaging	Het
Or8b3	A	G	9: 38,314,232 (GRCm39)	T18A	probably damaging	Het
Or8b42	A	T	9: 38,341,707 (GRCm39)	N43I	probably damaging	Het
Osbpl11	A	C	16: 33,030,353 (GRCm39)	I194L	probably benign	Het
Pacsin2	T	C	15: 83,269,252 (GRCm39)	E14G	probably damaging	Het
Pate7	T	C	9: 35,687,952 (GRCm39)	D96G	probably damaging	Het
Pcdh7	A	G	5: 57,877,764 (GRCm39)	T440A	probably damaging	Het
Pcdhb17	T	C	18: 37,618,878 (GRCm39)	S223P	probably damaging	Het
Pik3c2g	G	A	6: 139,693,904 (GRCm39)	R109K	probably benign	Het
Ppp2ca	T	C	11: 51,989,916 (GRCm39)	I14T	possibly damaging	Het
Rdh12	A	T	12: 79,258,278 (GRCm39)	T102S	probably damaging	Het
Rsf1	T	A	7: 97,288,520 (GRCm39)	C132*	probably null	Het
Rufy1	G	A	11: 50,285,755 (GRCm39)	L621F	probably damaging	Het
Ruvbl2	C	T	7: 45,074,135 (GRCm39)	R253H	possibly damaging	Het
Sdf2	A	G	11: 78,145,819 (GRCm39)	E172G	probably damaging	Het
Skint5	C	T	4: 113,856,651 (GRCm39)		probably null	Het
Slc25a27	A	G	17: 43,964,315 (GRCm39)	I185T	probably benign	Het
Slc41a3	A	C	6: 90,610,677 (GRCm39)	K180Q	probably benign	Het
Slc7a14	A	C	3: 31,291,598 (GRCm39)	F227V	probably damaging	Het
Smg1	T	C	7: 117,756,142 (GRCm39)		probably benign	Het
St6galnac1	A	T	11: 116,656,689 (GRCm39)	D483E	probably damaging	Het
Tes	A	G	6: 17,097,441 (GRCm39)	K183R	probably damaging	Het
Tgfbr1	G	T	4: 47,403,471 (GRCm39)	D290Y	probably damaging	Het
Tifab	T	A	13: 56,324,164 (GRCm39)	Y93F	probably benign	Het
Tmem131l	A	G	3: 83,848,196 (GRCm39)		probably null	Het
Tnfrsf21	T	A	17: 43,396,265 (GRCm39)	S516R	probably benign	Het
Ttn	T	C	2: 76,606,389 (GRCm39)	Y18140C	probably damaging	Het
Unc13b	T	A	4: 43,244,747 (GRCm39)	S3692T	probably damaging	Het
Vwa8	C	T	14: 79,145,670 (GRCm39)	R116C	probably damaging	Het
Zfp113	T	C	5: 138,149,459 (GRCm39)		probably benign	Het
Zfp26	T	C	9: 20,348,921 (GRCm39)	T548A	probably benign	Het
Zfp870	C	T	17: 33,102,990 (GRCm39)	G114D	probably damaging	Het

Other mutations in Mdn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Mdn1	APN	4	32,723,651 (GRCm39)	missense	probably damaging	1.00
IGL00426:Mdn1	APN	4	32,719,214 (GRCm39)	missense	possibly damaging	0.91
IGL00570:Mdn1	APN	4	32,735,719 (GRCm39)	missense	probably benign
IGL00573:Mdn1	APN	4	32,666,619 (GRCm39)	critical splice donor site	probably null
IGL00983:Mdn1	APN	4	32,735,525 (GRCm39)	missense	probably damaging	1.00
IGL01288:Mdn1	APN	4	32,730,864 (GRCm39)	missense	probably benign	0.00
IGL01359:Mdn1	APN	4	32,743,686 (GRCm39)	missense	probably benign	0.10
IGL01457:Mdn1	APN	4	32,715,922 (GRCm39)	missense	possibly damaging	0.82
IGL01530:Mdn1	APN	4	32,711,938 (GRCm39)	splice site	probably benign
IGL01684:Mdn1	APN	4	32,726,857 (GRCm39)	missense	probably benign
IGL01753:Mdn1	APN	4	32,708,483 (GRCm39)	missense	probably benign
IGL01901:Mdn1	APN	4	32,669,591 (GRCm39)	missense	probably damaging	1.00
IGL01952:Mdn1	APN	4	32,723,657 (GRCm39)	missense	possibly damaging	0.82
IGL01960:Mdn1	APN	4	32,758,393 (GRCm39)	missense	probably benign	0.14
IGL02019:Mdn1	APN	4	32,749,948 (GRCm39)	missense	possibly damaging	0.93
IGL02100:Mdn1	APN	4	32,715,708 (GRCm39)	missense	possibly damaging	0.90
IGL02117:Mdn1	APN	4	32,709,364 (GRCm39)	missense	probably benign	0.00
IGL02154:Mdn1	APN	4	32,740,395 (GRCm39)	missense	probably benign	0.35
IGL02216:Mdn1	APN	4	32,739,092 (GRCm39)	missense	probably benign	0.03
IGL02371:Mdn1	APN	4	32,676,860 (GRCm39)	splice site	probably benign
IGL02396:Mdn1	APN	4	32,700,120 (GRCm39)	missense	probably damaging	0.99
IGL02454:Mdn1	APN	4	32,694,674 (GRCm39)	critical splice donor site	probably null
IGL02502:Mdn1	APN	4	32,670,579 (GRCm39)	missense	possibly damaging	0.69
IGL02883:Mdn1	APN	4	32,763,199 (GRCm39)	missense	probably benign	0.05
IGL02946:Mdn1	APN	4	32,734,366 (GRCm39)	missense	probably damaging	0.98
IGL02950:Mdn1	APN	4	32,713,360 (GRCm39)	splice site	probably benign
IGL03076:Mdn1	APN	4	32,735,564 (GRCm39)	missense	probably damaging	0.97
IGL03129:Mdn1	APN	4	32,729,994 (GRCm39)	missense	possibly damaging	0.47
IGL03234:Mdn1	APN	4	32,732,842 (GRCm39)	missense	probably benign	0.06
3-1:Mdn1	UTSW	4	32,725,967 (GRCm39)	critical splice donor site	probably null
IGL03046:Mdn1	UTSW	4	32,694,495 (GRCm39)	missense	possibly damaging	0.73
P0035:Mdn1	UTSW	4	32,749,934 (GRCm39)	missense	probably benign	0.05
PIT4508001:Mdn1	UTSW	4	32,719,223 (GRCm39)	missense	probably damaging	0.97
PIT4618001:Mdn1	UTSW	4	32,746,527 (GRCm39)	missense	probably benign	0.20
R0008:Mdn1	UTSW	4	32,718,317 (GRCm39)	missense	possibly damaging	0.47
R0110:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0125:Mdn1	UTSW	4	32,729,956 (GRCm39)	missense	probably damaging	0.98
R0257:Mdn1	UTSW	4	32,693,534 (GRCm39)	missense	probably damaging	0.99
R0266:Mdn1	UTSW	4	32,741,835 (GRCm39)	missense	probably damaging	0.99
R0349:Mdn1	UTSW	4	32,750,318 (GRCm39)	missense	probably damaging	1.00
R0362:Mdn1	UTSW	4	32,746,439 (GRCm39)	critical splice acceptor site	probably null
R0421:Mdn1	UTSW	4	32,684,707 (GRCm39)	missense	probably benign	0.39
R0450:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0465:Mdn1	UTSW	4	32,699,204 (GRCm39)	splice site	probably benign
R0469:Mdn1	UTSW	4	32,738,619 (GRCm39)	missense	probably benign	0.20
R0477:Mdn1	UTSW	4	32,750,928 (GRCm39)	missense	probably benign	0.02
R0481:Mdn1	UTSW	4	32,767,182 (GRCm39)	splice site	probably benign
R0504:Mdn1	UTSW	4	32,698,916 (GRCm39)	splice site	probably benign
R0522:Mdn1	UTSW	4	32,672,837 (GRCm39)	missense	probably benign	0.09
R0550:Mdn1	UTSW	4	32,730,479 (GRCm39)	missense	probably benign	0.13
R0607:Mdn1	UTSW	4	32,732,829 (GRCm39)	missense	probably benign	0.36
R0607:Mdn1	UTSW	4	32,712,014 (GRCm39)	missense	probably damaging	1.00
R0664:Mdn1	UTSW	4	32,768,011 (GRCm39)	nonsense	probably null
R0701:Mdn1	UTSW	4	32,699,263 (GRCm39)	missense	probably benign	0.00
R0801:Mdn1	UTSW	4	32,668,895 (GRCm39)	missense	probably benign	0.04
R0841:Mdn1	UTSW	4	32,752,032 (GRCm39)	missense	probably benign	0.23
R0849:Mdn1	UTSW	4	32,741,835 (GRCm39)	missense	probably damaging	0.99
R0893:Mdn1	UTSW	4	32,701,713 (GRCm39)	missense	probably benign	0.01
R1114:Mdn1	UTSW	4	32,746,568 (GRCm39)	critical splice donor site	probably null
R1137:Mdn1	UTSW	4	32,694,511 (GRCm39)	missense	probably damaging	1.00
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32,735,576 (GRCm39)	missense	possibly damaging	0.94
R1257:Mdn1	UTSW	4	32,667,089 (GRCm39)	critical splice acceptor site	probably null
R1356:Mdn1	UTSW	4	32,700,334 (GRCm39)	splice site	probably benign
R1466:Mdn1	UTSW	4	32,730,788 (GRCm39)	missense	probably benign	0.28
R1466:Mdn1	UTSW	4	32,730,788 (GRCm39)	missense	probably benign	0.28
R1518:Mdn1	UTSW	4	32,739,977 (GRCm39)	missense	probably damaging	1.00
R1569:Mdn1	UTSW	4	32,723,501 (GRCm39)	missense	probably null	0.10
R1574:Mdn1	UTSW	4	32,722,315 (GRCm39)	missense	probably benign
R1574:Mdn1	UTSW	4	32,722,315 (GRCm39)	missense	probably benign
R1678:Mdn1	UTSW	4	32,663,050 (GRCm39)	missense	probably damaging	0.99
R1696:Mdn1	UTSW	4	32,700,417 (GRCm39)	missense	possibly damaging	0.91
R1707:Mdn1	UTSW	4	32,693,504 (GRCm39)	missense	probably damaging	1.00
R1749:Mdn1	UTSW	4	32,773,952 (GRCm39)	missense	probably damaging	1.00
R1780:Mdn1	UTSW	4	32,700,103 (GRCm39)	missense	probably damaging	1.00
R1833:Mdn1	UTSW	4	32,720,761 (GRCm39)	missense	probably damaging	0.97
R1858:Mdn1	UTSW	4	32,730,881 (GRCm39)	missense	probably benign	0.17
R1870:Mdn1	UTSW	4	32,763,339 (GRCm39)	missense	probably damaging	1.00
R1887:Mdn1	UTSW	4	32,742,540 (GRCm39)	missense	probably damaging	1.00
R1909:Mdn1	UTSW	4	32,760,839 (GRCm39)	small deletion	probably benign
R2075:Mdn1	UTSW	4	32,716,058 (GRCm39)	missense	probably benign	0.03
R2103:Mdn1	UTSW	4	32,738,712 (GRCm39)	missense	possibly damaging	0.75
R2104:Mdn1	UTSW	4	32,743,843 (GRCm39)	splice site	probably null
R2110:Mdn1	UTSW	4	32,700,409 (GRCm39)	missense	probably damaging	1.00
R2111:Mdn1	UTSW	4	32,700,409 (GRCm39)	missense	probably damaging	1.00
R2206:Mdn1	UTSW	4	32,716,271 (GRCm39)	missense	possibly damaging	0.71
R2221:Mdn1	UTSW	4	32,763,306 (GRCm39)	missense	probably benign	0.37
R2240:Mdn1	UTSW	4	32,765,701 (GRCm39)	missense	possibly damaging	0.90
R2351:Mdn1	UTSW	4	32,750,010 (GRCm39)	missense	probably benign	0.21
R2421:Mdn1	UTSW	4	32,723,621 (GRCm39)	missense	probably damaging	0.96
R3036:Mdn1	UTSW	4	32,750,013 (GRCm39)	missense	probably damaging	0.99
R3434:Mdn1	UTSW	4	32,733,726 (GRCm39)	critical splice donor site	probably null
R3435:Mdn1	UTSW	4	32,733,726 (GRCm39)	critical splice donor site	probably null
R3783:Mdn1	UTSW	4	32,720,818 (GRCm39)	missense	probably benign	0.01
R3811:Mdn1	UTSW	4	32,693,506 (GRCm39)	nonsense	probably null
R3973:Mdn1	UTSW	4	32,722,363 (GRCm39)	missense	probably benign	0.00
R4154:Mdn1	UTSW	4	32,707,475 (GRCm39)	missense	probably damaging	0.96
R4372:Mdn1	UTSW	4	32,743,809 (GRCm39)	missense	probably benign	0.03
R4393:Mdn1	UTSW	4	32,754,482 (GRCm39)	missense	possibly damaging	0.48
R4438:Mdn1	UTSW	4	32,704,635 (GRCm39)	missense	probably damaging	1.00
R4471:Mdn1	UTSW	4	32,668,860 (GRCm39)	missense	probably benign	0.00
R4509:Mdn1	UTSW	4	32,715,883 (GRCm39)	missense	probably damaging	1.00
R4538:Mdn1	UTSW	4	32,722,334 (GRCm39)	missense	probably damaging	1.00
R4557:Mdn1	UTSW	4	32,754,437 (GRCm39)	missense	probably damaging	1.00
R4570:Mdn1	UTSW	4	32,741,812 (GRCm39)	missense	probably damaging	1.00
R4591:Mdn1	UTSW	4	32,707,636 (GRCm39)	missense	probably damaging	1.00
R4658:Mdn1	UTSW	4	32,730,749 (GRCm39)	splice site	probably null
R4667:Mdn1	UTSW	4	32,679,572 (GRCm39)	missense	probably damaging	1.00
R4684:Mdn1	UTSW	4	32,666,430 (GRCm39)	missense	probably damaging	1.00
R4778:Mdn1	UTSW	4	32,683,583 (GRCm39)	nonsense	probably null
R4807:Mdn1	UTSW	4	32,685,651 (GRCm39)	splice site	probably null
R4923:Mdn1	UTSW	4	32,671,608 (GRCm39)	missense	possibly damaging	0.89
R4951:Mdn1	UTSW	4	32,707,459 (GRCm39)	missense	probably damaging	1.00
R4963:Mdn1	UTSW	4	32,756,512 (GRCm39)	missense	probably benign	0.00
R4971:Mdn1	UTSW	4	32,739,827 (GRCm39)	missense	probably damaging	1.00
R4973:Mdn1	UTSW	4	32,734,418 (GRCm39)	missense	probably benign	0.01
R5122:Mdn1	UTSW	4	32,670,593 (GRCm39)	missense	probably damaging	1.00
R5159:Mdn1	UTSW	4	32,774,008 (GRCm39)	missense	possibly damaging	0.93
R5164:Mdn1	UTSW	4	32,759,011 (GRCm39)	splice site	probably null
R5215:Mdn1	UTSW	4	32,741,418 (GRCm39)	missense	possibly damaging	0.78
R5217:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5219:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5365:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5366:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5368:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5445:Mdn1	UTSW	4	32,723,690 (GRCm39)	missense	probably damaging	0.98
R5462:Mdn1	UTSW	4	32,720,897 (GRCm39)	missense	probably benign
R5522:Mdn1	UTSW	4	32,685,783 (GRCm39)	missense	probably damaging	1.00
R5525:Mdn1	UTSW	4	32,767,961 (GRCm39)	missense	possibly damaging	0.73
R5578:Mdn1	UTSW	4	32,728,167 (GRCm39)	missense	probably benign	0.04
R5605:Mdn1	UTSW	4	32,765,664 (GRCm39)	missense	probably benign
R5621:Mdn1	UTSW	4	32,716,371 (GRCm39)	missense	possibly damaging	0.46
R5636:Mdn1	UTSW	4	32,695,480 (GRCm39)	missense	probably damaging	1.00
R5650:Mdn1	UTSW	4	32,667,467 (GRCm39)	splice site	probably null
R5780:Mdn1	UTSW	4	32,722,950 (GRCm39)	missense	probably benign	0.02
R5838:Mdn1	UTSW	4	32,754,547 (GRCm39)	missense	probably damaging	0.99
R5857:Mdn1	UTSW	4	32,670,646 (GRCm39)	missense	probably benign	0.09
R5895:Mdn1	UTSW	4	32,695,400 (GRCm39)	missense	probably damaging	1.00
R5943:Mdn1	UTSW	4	32,678,330 (GRCm39)	missense	probably damaging	1.00
R6008:Mdn1	UTSW	4	32,741,073 (GRCm39)	missense	probably damaging	1.00
R6013:Mdn1	UTSW	4	32,715,713 (GRCm39)	missense	probably damaging	1.00
R6075:Mdn1	UTSW	4	32,689,581 (GRCm39)	missense	possibly damaging	0.48
R6151:Mdn1	UTSW	4	32,684,735 (GRCm39)	missense	probably damaging	1.00
R6163:Mdn1	UTSW	4	32,716,040 (GRCm39)	missense	probably damaging	1.00
R6181:Mdn1	UTSW	4	32,715,953 (GRCm39)	missense	probably damaging	1.00
R6211:Mdn1	UTSW	4	32,696,269 (GRCm39)	missense	probably benign	0.12
R6249:Mdn1	UTSW	4	32,708,484 (GRCm39)	missense	possibly damaging	0.85
R6251:Mdn1	UTSW	4	32,748,590 (GRCm39)	missense	probably benign	0.13
R6253:Mdn1	UTSW	4	32,749,593 (GRCm39)	missense	probably benign	0.25
R6273:Mdn1	UTSW	4	32,715,979 (GRCm39)	missense	probably benign	0.01
R6297:Mdn1	UTSW	4	32,730,054 (GRCm39)	nonsense	probably null
R6384:Mdn1	UTSW	4	32,670,607 (GRCm39)	missense	probably damaging	1.00
R6463:Mdn1	UTSW	4	32,773,308 (GRCm39)	missense	probably damaging	1.00
R6528:Mdn1	UTSW	4	32,713,780 (GRCm39)	missense	probably damaging	1.00
R6688:Mdn1	UTSW	4	32,774,041 (GRCm39)	missense	possibly damaging	0.74
R6762:Mdn1	UTSW	4	32,676,786 (GRCm39)	missense	possibly damaging	0.50
R6794:Mdn1	UTSW	4	32,741,893 (GRCm39)	missense	probably damaging	1.00
R6894:Mdn1	UTSW	4	32,748,614 (GRCm39)	missense	possibly damaging	0.75
R6935:Mdn1	UTSW	4	32,774,041 (GRCm39)	missense	possibly damaging	0.74
R6980:Mdn1	UTSW	4	32,726,942 (GRCm39)	critical splice donor site	probably null
R6995:Mdn1	UTSW	4	32,733,374 (GRCm39)	missense	probably benign	0.03
R7048:Mdn1	UTSW	4	32,767,969 (GRCm39)	missense	probably benign	0.00
R7082:Mdn1	UTSW	4	32,762,341 (GRCm39)	missense	probably benign
R7158:Mdn1	UTSW	4	32,725,121 (GRCm39)	missense	probably benign	0.09
R7166:Mdn1	UTSW	4	32,746,446 (GRCm39)	missense	probably damaging	1.00
R7168:Mdn1	UTSW	4	32,719,184 (GRCm39)	missense	probably damaging	1.00
R7175:Mdn1	UTSW	4	32,694,634 (GRCm39)	missense	probably damaging	1.00
R7195:Mdn1	UTSW	4	32,701,823 (GRCm39)	missense	probably damaging	1.00
R7250:Mdn1	UTSW	4	32,695,427 (GRCm39)	missense	probably damaging	1.00
R7274:Mdn1	UTSW	4	32,725,944 (GRCm39)	missense	probably benign	0.12
R7330:Mdn1	UTSW	4	32,723,685 (GRCm39)	missense	probably benign	0.16
R7363:Mdn1	UTSW	4	32,691,729 (GRCm39)	missense	probably damaging	0.99
R7369:Mdn1	UTSW	4	32,773,375 (GRCm39)	missense	probably damaging	0.99
R7452:Mdn1	UTSW	4	32,739,030 (GRCm39)	missense	possibly damaging	0.87
R7523:Mdn1	UTSW	4	32,667,270 (GRCm39)	critical splice acceptor site	probably null
R7594:Mdn1	UTSW	4	32,696,359 (GRCm39)	missense	probably benign	0.27
R7605:Mdn1	UTSW	4	32,694,599 (GRCm39)	missense	probably damaging	1.00
R7661:Mdn1	UTSW	4	32,691,229 (GRCm39)	missense	probably benign	0.08
R7689:Mdn1	UTSW	4	32,739,912 (GRCm39)	missense	probably damaging	1.00
R7699:Mdn1	UTSW	4	32,741,344 (GRCm39)	missense	probably damaging	1.00
R7700:Mdn1	UTSW	4	32,741,344 (GRCm39)	missense	probably damaging	1.00
R7714:Mdn1	UTSW	4	32,722,360 (GRCm39)	missense	possibly damaging	0.75
R7718:Mdn1	UTSW	4	32,718,420 (GRCm39)	missense	probably damaging	1.00
R7762:Mdn1	UTSW	4	32,734,421 (GRCm39)	missense	probably benign
R7787:Mdn1	UTSW	4	32,741,794 (GRCm39)	missense	probably damaging	1.00
R8111:Mdn1	UTSW	4	32,674,003 (GRCm39)	missense	possibly damaging	0.81
R8222:Mdn1	UTSW	4	32,707,477 (GRCm39)	missense	probably benign	0.09
R8246:Mdn1	UTSW	4	32,657,284 (GRCm39)	missense	probably benign	0.06
R8267:Mdn1	UTSW	4	32,742,485 (GRCm39)	missense	possibly damaging	0.82
R8286:Mdn1	UTSW	4	32,731,960 (GRCm39)	missense	possibly damaging	0.91
R8305:Mdn1	UTSW	4	32,725,107 (GRCm39)	missense	probably benign
R8318:Mdn1	UTSW	4	32,735,897 (GRCm39)	critical splice donor site	probably null
R8379:Mdn1	UTSW	4	32,756,453 (GRCm39)	missense	probably null	1.00
R8384:Mdn1	UTSW	4	32,765,680 (GRCm39)	missense	probably benign	0.05
R8514:Mdn1	UTSW	4	32,739,857 (GRCm39)	missense	probably damaging	1.00
R8560:Mdn1	UTSW	4	32,743,830 (GRCm39)	missense	probably benign	0.08
R8672:Mdn1	UTSW	4	32,768,793 (GRCm39)	missense	probably damaging	1.00
R8708:Mdn1	UTSW	4	32,725,854 (GRCm39)	missense	probably damaging	1.00
R8769:Mdn1	UTSW	4	32,751,390 (GRCm39)	missense	probably damaging	0.97
R8896:Mdn1	UTSW	4	32,678,328 (GRCm39)	missense	probably benign	0.28
R8918:Mdn1	UTSW	4	32,744,579 (GRCm39)	nonsense	probably null
R8920:Mdn1	UTSW	4	32,719,280 (GRCm39)	missense	probably damaging	1.00
R8966:Mdn1	UTSW	4	32,672,837 (GRCm39)	nonsense	probably null
R8997:Mdn1	UTSW	4	32,773,275 (GRCm39)	missense	probably damaging	1.00
R9120:Mdn1	UTSW	4	32,701,814 (GRCm39)	missense	probably damaging	1.00
R9129:Mdn1	UTSW	4	32,676,812 (GRCm39)	missense	probably benign	0.24
R9131:Mdn1	UTSW	4	32,762,275 (GRCm39)	missense	possibly damaging	0.69
R9200:Mdn1	UTSW	4	32,760,791 (GRCm39)	missense	probably benign	0.00
R9226:Mdn1	UTSW	4	32,694,612 (GRCm39)	missense	probably benign	0.25
R9235:Mdn1	UTSW	4	32,739,122 (GRCm39)	missense	probably benign	0.10
R9293:Mdn1	UTSW	4	32,707,579 (GRCm39)	missense	probably damaging	1.00
R9315:Mdn1	UTSW	4	32,760,911 (GRCm39)	missense	probably benign	0.00
R9338:Mdn1	UTSW	4	32,666,536 (GRCm39)	missense	probably benign	0.00
R9353:Mdn1	UTSW	4	32,693,504 (GRCm39)	missense	probably damaging	1.00
R9393:Mdn1	UTSW	4	32,713,825 (GRCm39)	missense
R9420:Mdn1	UTSW	4	32,678,414 (GRCm39)	missense	probably damaging	1.00
R9475:Mdn1	UTSW	4	32,739,849 (GRCm39)	missense	possibly damaging	0.65
R9583:Mdn1	UTSW	4	32,741,372 (GRCm39)	missense	probably damaging	1.00
R9600:Mdn1	UTSW	4	32,684,723 (GRCm39)	nonsense	probably null
R9640:Mdn1	UTSW	4	32,754,539 (GRCm39)	missense	probably damaging	1.00
R9688:Mdn1	UTSW	4	32,745,590 (GRCm39)	missense	probably damaging	1.00
R9744:Mdn1	UTSW	4	32,715,711 (GRCm39)	missense	possibly damaging	0.91
X0066:Mdn1	UTSW	4	32,739,030 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,696,244 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,668,944 (GRCm39)	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32,667,102 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ACCATCTGAGAGGTGCTGCTGTTG -3'
(R):5'- GAAGAGTCTTGTGTCCAGTTCGTCC -3'

Sequencing Primer
(F):5'- TGTACAGGGAAACACATAAACCTGAG -3'
(R):5'- TCCTATGATTTGGAGAAGCAGC -3'

Posted On

2014-04-24