Mutagenetix > Incidental Mutation

Incidental Mutation 'R1607:Or2w4'

176578

Institutional Source

Beutler Lab

Gene Symbol

Or2w4

Ensembl Gene

ENSMUSG00000051258

Gene Name

olfactory receptor family 2 subfamily W member 4

Synonyms

Olfr1363-ps1, Olfr1362, GA_x6K02T2QHY8-11617756-11618682, GA_x6K02T2QHY8-11628399-11628868, MOR256-8

MMRRC Submission

039644-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.138) question?

Stock #

R1607 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21795190-21796174 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 21795934 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 68 (F68L)

Ref Sequence

ENSEMBL: ENSMUSP00000061660 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051874]

AlphaFold

Q5SZZ8

Predicted Effect

probably benign
Transcript: ENSMUST00000051874
AA Change: F68L

PolyPhen 2 Score 0.041 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000061660
Gene: ENSMUSG00000051258
AA Change: F68L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	307	3.5e-51	PFAM
Pfam:7tm_1	41	290	9e-24	PFAM

Coding Region Coverage

1x: 98.9%
3x: 98.0%
10x: 95.1%
20x: 88.5%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	C	T	7: 119,850,514 (GRCm39)	P789S	probably damaging	Het
Adam17	T	A	12: 21,384,139 (GRCm39)		probably null	Het
Afdn	C	T	17: 14,030,763 (GRCm39)	R224W	probably damaging	Het
Als2	A	G	1: 59,219,306 (GRCm39)	Y1215H	probably damaging	Het
Anks1b	A	T	10: 89,878,410 (GRCm39)	D73V	probably damaging	Het
Bpifb6	T	C	2: 153,748,781 (GRCm39)	F259S	probably damaging	Het
Cenpv	A	T	11: 62,416,002 (GRCm39)	M249K	probably benign	Het
Ces1d	C	T	8: 93,912,746 (GRCm39)	V231I	probably benign	Het
Clasp1	G	A	1: 118,432,689 (GRCm39)	V460I	probably damaging	Het
Cnot10	A	T	9: 114,458,163 (GRCm39)	Y114*	probably null	Het
Cpb1	G	T	3: 20,317,946 (GRCm39)	R193S	probably benign	Het
Ctc1	T	C	11: 68,926,976 (GRCm39)	S1207P	possibly damaging	Het
Cttnbp2	T	A	6: 18,435,432 (GRCm39)	K142M	probably damaging	Het
Dhrs7b	T	G	11: 60,742,717 (GRCm39)	D136E	probably benign	Het
Dnah7b	T	C	1: 46,329,806 (GRCm39)	S3217P	probably damaging	Het
Eif4g3	C	T	4: 137,853,874 (GRCm39)	S480L	probably benign	Het
Enah	A	T	1: 181,744,762 (GRCm39)		probably null	Het
Epx	T	C	11: 87,759,538 (GRCm39)	D517G	probably damaging	Het
Gabrg2	T	A	11: 41,867,490 (GRCm39)	D43V	probably damaging	Het
Gdf9	A	T	11: 53,328,338 (GRCm39)	E431D	possibly damaging	Het
Ghr	T	C	15: 3,350,056 (GRCm39)	D374G	probably damaging	Het
Gin1	A	T	1: 97,713,875 (GRCm39)	I392F	probably damaging	Het
Greb1l	A	G	18: 10,529,703 (GRCm39)	E786G	possibly damaging	Het
Grk4	T	C	5: 34,888,882 (GRCm39)	V342A	probably benign	Het
Hhipl2	A	T	1: 183,204,432 (GRCm39)	Y135F	possibly damaging	Het
Insig1	C	T	5: 28,276,706 (GRCm39)	R91W	probably damaging	Het
Kcnf1	C	G	12: 17,225,733 (GRCm39)	D163H	probably benign	Het
Kif11	A	T	19: 37,375,648 (GRCm39)	K190*	probably null	Het
Ldb2	T	G	5: 44,630,814 (GRCm39)	E309A	probably damaging	Het
Lipo4	T	G	19: 33,490,073 (GRCm39)	D143A	probably damaging	Het
Map3k6	T	G	4: 132,979,784 (GRCm39)	I1261S	probably damaging	Het
Mtus1	C	T	8: 41,468,446 (GRCm39)	V27I	possibly damaging	Het
Ndufaf2	C	T	13: 108,228,107 (GRCm39)	V60I	probably benign	Het
Nup133	A	G	8: 124,675,774 (GRCm39)	F48L	probably benign	Het
Or1i2	G	T	10: 78,447,974 (GRCm39)	T167N	probably benign	Het
Or5w11	T	A	2: 87,459,321 (GRCm39)	N171K	probably benign	Het
Pcdhb21	A	T	18: 37,648,532 (GRCm39)	N554Y	probably damaging	Het
Pcx	A	T	19: 4,653,187 (GRCm39)	D284V	possibly damaging	Het
Pcyt1a	T	C	16: 32,285,937 (GRCm39)	S203P	probably damaging	Het
Pdlim3	A	T	8: 46,349,896 (GRCm39)	I69F	probably damaging	Het
Pkp4	T	C	2: 59,152,898 (GRCm39)	V598A	probably benign	Het
Plbd1	T	C	6: 136,589,304 (GRCm39)	I509V	probably benign	Het
Polr3b	C	A	10: 84,488,647 (GRCm39)	T319N	probably benign	Het
Ptprj	T	C	2: 90,293,664 (GRCm39)	D380G	probably benign	Het
Ralgapa1	T	G	12: 55,788,321 (GRCm39)	R587S	probably damaging	Het
Scn5a	C	A	9: 119,315,158 (GRCm39)	R1850L	probably damaging	Het
Ssc5d	C	A	7: 4,947,042 (GRCm39)	T1132K	probably benign	Het
Stard7	T	A	2: 127,137,406 (GRCm39)	N285K	possibly damaging	Het
Tcp11l2	C	T	10: 84,449,351 (GRCm39)	R439W	probably damaging	Het
Tecrl	T	C	5: 83,428,355 (GRCm39)		probably null	Het
Tep1	A	T	14: 51,062,020 (GRCm39)	L2570Q	probably null	Het
Tex14	A	T	11: 87,445,754 (GRCm39)	D191V	probably damaging	Het
Tm7sf2	A	G	19: 6,113,049 (GRCm39)		probably null	Het
Tmem132e	A	G	11: 82,328,196 (GRCm39)	K408R	probably benign	Het
Tube1	A	G	10: 39,020,762 (GRCm39)	D216G	possibly damaging	Het
Ubap2	C	T	4: 41,199,872 (GRCm39)	A752T	probably benign	Het
Vmn2r82	A	G	10: 79,215,253 (GRCm39)	H412R	possibly damaging	Het
Wtip	T	C	7: 33,816,020 (GRCm39)	E352G	probably damaging	Het
Xirp2	T	A	2: 67,340,639 (GRCm39)	L960*	probably null	Het
Zc3h11a	A	C	1: 133,552,425 (GRCm39)	S561A	probably benign	Het
Zfhx2	A	T	14: 55,300,442 (GRCm39)	D2436E	probably damaging	Het
Zfp763	T	C	17: 33,238,881 (GRCm39)	H88R	probably benign	Het

Other mutations in Or2w4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00949:Or2w4	APN	13	21,795,521 (GRCm39)	missense	probably damaging	0.99
R0070:Or2w4	UTSW	13	21,795,431 (GRCm39)	missense	possibly damaging	0.60
R0070:Or2w4	UTSW	13	21,795,431 (GRCm39)	missense	possibly damaging	0.60
R1458:Or2w4	UTSW	13	21,795,992 (GRCm39)	missense	probably benign	0.37
R1587:Or2w4	UTSW	13	21,796,083 (GRCm39)	missense	probably benign	0.01
R1588:Or2w4	UTSW	13	21,796,083 (GRCm39)	missense	probably benign	0.01
R1708:Or2w4	UTSW	13	21,795,240 (GRCm39)	missense	probably damaging	0.98
R4633:Or2w4	UTSW	13	21,795,398 (GRCm39)	missense	probably damaging	0.99
R6223:Or2w4	UTSW	13	21,795,536 (GRCm39)	missense	probably benign
R6229:Or2w4	UTSW	13	21,795,819 (GRCm39)	missense	probably benign
R7140:Or2w4	UTSW	13	21,795,297 (GRCm39)	missense	possibly damaging	0.87
R8385:Or2w4	UTSW	13	21,795,522 (GRCm39)	missense	probably benign	0.12
R9433:Or2w4	UTSW	13	21,795,224 (GRCm39)	missense	probably benign	0.04
R9654:Or2w4	UTSW	13	21,795,915 (GRCm39)	missense	possibly damaging	0.93
R9744:Or2w4	UTSW	13	21,795,827 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- TGACATGATGATGGCATTGGCGAG -3'
(R):5'- TAGAAGGCTTCTCTGATCGCCCTC -3'

Sequencing Primer
(F):5'- AGCTGTGGGTGCATAATGACC -3'
(R):5'- TCGCCTGGAGATGGTACTC -3'

Posted On

2014-04-24