Mutagenetix > Incidental Mutation

Incidental Mutation 'R1614:Vmn2r27'

176993

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r27

Ensembl Gene

ENSMUSG00000072778

Gene Name

vomeronasal 2, receptor27

Synonyms

EG232367

MMRRC Submission

039651-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.061) question?

Stock #

R1614 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

124168555-124208743 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 124200893 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 355 (I355L)

Ref Sequence

ENSEMBL: ENSMUSP00000098528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100968]

AlphaFold

D3YUK6

Predicted Effect

probably benign
Transcript: ENSMUST00000100968
AA Change: I355L

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: I355L

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	81	475	1.1e-27	PFAM
Pfam:NCD3G	519	570	1.3e-18	PFAM
Pfam:7tm_3	603	838	2.6e-50	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.4%
20x: 89.4%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arl9	A	G	5: 77,158,412 (GRCm39)	T165A	probably benign	Het
Atpaf1	A	T	4: 115,653,954 (GRCm39)	K201N	possibly damaging	Het
Cacna1b	G	T	2: 24,580,819 (GRCm39)	Q676K	possibly damaging	Het
Ccdc88c	A	T	12: 100,879,243 (GRCm39)	H1959Q	probably benign	Het
Cep162	T	C	9: 87,094,985 (GRCm39)	D808G	probably damaging	Het
Chct1	T	G	11: 85,063,690 (GRCm39)	S28A	possibly damaging	Het
Chtf18	A	G	17: 25,946,064 (GRCm39)	L42P	probably benign	Het
Cox7c	A	G	13: 86,193,904 (GRCm39)	F40L	probably benign	Het
Dock7	C	T	4: 98,949,517 (GRCm39)	V442I	probably benign	Het
Dst	T	C	1: 34,314,344 (GRCm39)	F4198S	probably damaging	Het
Fam13a	T	A	6: 58,917,169 (GRCm39)	D569V	probably damaging	Het
Gm6741	T	A	17: 91,544,424 (GRCm39)	H62Q	probably benign	Het
Gnptab	A	G	10: 88,250,451 (GRCm39)	T172A	probably benign	Het
Greb1	A	G	12: 16,751,172 (GRCm39)	S1013P	probably damaging	Het
Insl5	A	T	4: 102,883,846 (GRCm39)	L25*	probably null	Het
Ipo13	A	G	4: 117,761,815 (GRCm39)	S462P	probably benign	Het
Itgb1	G	A	8: 129,446,546 (GRCm39)	C401Y	probably damaging	Het
Kcnh7	G	T	2: 62,680,948 (GRCm39)	A213E	probably benign	Het
Kcnv1	G	A	15: 44,977,840 (GRCm39)	T66M	probably damaging	Het
Mesp2	T	C	7: 79,461,367 (GRCm39)	S231P	probably benign	Het
Nabp1	T	C	1: 51,510,511 (GRCm39)	N164D	possibly damaging	Het
Nop53	A	G	7: 15,679,890 (GRCm39)	V30A	probably benign	Het
Or1j17	T	G	2: 36,578,321 (GRCm39)	Y102*	probably null	Het
Or4a73	T	A	2: 89,421,040 (GRCm39)	I140L	possibly damaging	Het
Or4f54	T	A	2: 111,123,411 (GRCm39)	V266E	probably damaging	Het
Or4f62	T	A	2: 111,986,862 (GRCm39)	C189S	probably damaging	Het
Pcsk5	G	A	19: 17,492,620 (GRCm39)	R918C	probably damaging	Het
Pecam1	T	C	11: 106,571,905 (GRCm39)	D554G	probably benign	Het
Polr2a	T	C	11: 69,634,199 (GRCm39)	I744V	possibly damaging	Het
Pop1	C	A	15: 34,530,356 (GRCm39)	A918D	possibly damaging	Het
Ppp2r5e	C	G	12: 75,516,341 (GRCm39)	A239P	probably damaging	Het
Prmt3	A	T	7: 49,476,467 (GRCm39)	I359F	possibly damaging	Het
Proz	G	A	8: 13,116,904 (GRCm39)	C152Y	probably damaging	Het
Ptgfr	A	C	3: 151,507,416 (GRCm39)	Y316D	probably benign	Het
Ralgapa2	G	T	2: 146,230,532 (GRCm39)	S1011Y	probably damaging	Het
Rnf43	C	T	11: 87,622,485 (GRCm39)	R529*	probably null	Het
Slc17a6	G	A	7: 51,296,025 (GRCm39)		probably benign	Het
Slc25a19	A	T	11: 115,507,449 (GRCm39)	C224*	probably null	Het
Smarcd3	A	G	5: 24,799,874 (GRCm39)	S299P	possibly damaging	Het
Stard9	T	C	2: 120,528,156 (GRCm39)	F1471S	possibly damaging	Het
Strada	A	C	11: 106,059,145 (GRCm39)	V211G	probably damaging	Het
Tom1l1	T	C	11: 90,574,080 (GRCm39)	E68G	probably damaging	Het
Vmn2r68	A	T	7: 84,870,946 (GRCm39)	M779K	possibly damaging	Het
Zbtb18	T	C	1: 177,274,736 (GRCm39)	L23P	probably damaging	Het
Zfp112	G	T	7: 23,826,024 (GRCm39)	C664F	probably damaging	Het
Zfp955a	A	T	17: 33,461,306 (GRCm39)	N275K	possibly damaging	Het

Other mutations in Vmn2r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Vmn2r27	APN	6	124,169,370 (GRCm39)	missense	possibly damaging	0.86
IGL01388:Vmn2r27	APN	6	124,200,791 (GRCm39)	missense	possibly damaging	0.55
IGL01923:Vmn2r27	APN	6	124,177,484 (GRCm39)	missense	probably benign	0.20
IGL01954:Vmn2r27	APN	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
IGL02105:Vmn2r27	APN	6	124,174,308 (GRCm39)	splice site	probably benign
IGL02586:Vmn2r27	APN	6	124,201,434 (GRCm39)	nonsense	probably null
IGL03130:Vmn2r27	APN	6	124,169,276 (GRCm39)	missense	possibly damaging	0.82
IGL03330:Vmn2r27	APN	6	124,207,139 (GRCm39)	nonsense	probably null
R0124:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0234:Vmn2r27	UTSW	6	124,208,578 (GRCm39)	missense	probably benign
R0384:Vmn2r27	UTSW	6	124,200,871 (GRCm39)	missense	probably benign	0.01
R0582:Vmn2r27	UTSW	6	124,201,249 (GRCm39)	missense	probably benign	0.02
R0733:Vmn2r27	UTSW	6	124,169,147 (GRCm39)	missense	probably benign	0.18
R0738:Vmn2r27	UTSW	6	124,200,661 (GRCm39)	missense	possibly damaging	0.48
R0835:Vmn2r27	UTSW	6	124,177,583 (GRCm39)	missense	probably damaging	0.99
R1183:Vmn2r27	UTSW	6	124,177,491 (GRCm39)	missense	probably benign
R1401:Vmn2r27	UTSW	6	124,168,591 (GRCm39)	nonsense	probably null
R1484:Vmn2r27	UTSW	6	124,177,474 (GRCm39)	missense	probably damaging	0.96
R1536:Vmn2r27	UTSW	6	124,177,649 (GRCm39)	missense	probably damaging	1.00
R1539:Vmn2r27	UTSW	6	124,168,730 (GRCm39)	missense	probably damaging	1.00
R1565:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1595:Vmn2r27	UTSW	6	124,208,574 (GRCm39)	missense	probably benign	0.00
R1742:Vmn2r27	UTSW	6	124,177,636 (GRCm39)	missense	possibly damaging	0.48
R1816:Vmn2r27	UTSW	6	124,207,330 (GRCm39)	nonsense	probably null
R1822:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1824:Vmn2r27	UTSW	6	124,208,593 (GRCm39)	missense	probably benign
R1870:Vmn2r27	UTSW	6	124,201,170 (GRCm39)	missense	probably benign	0.11
R1942:Vmn2r27	UTSW	6	124,200,722 (GRCm39)	missense	probably damaging	1.00
R1962:Vmn2r27	UTSW	6	124,200,793 (GRCm39)	missense	possibly damaging	0.70
R2069:Vmn2r27	UTSW	6	124,201,442 (GRCm39)	missense	probably damaging	1.00
R2075:Vmn2r27	UTSW	6	124,177,510 (GRCm39)	missense	possibly damaging	0.85
R2379:Vmn2r27	UTSW	6	124,201,342 (GRCm39)	missense	possibly damaging	0.89
R3748:Vmn2r27	UTSW	6	124,207,351 (GRCm39)	missense	probably benign	0.35
R4384:Vmn2r27	UTSW	6	124,201,115 (GRCm39)	missense	probably benign	0.05
R4392:Vmn2r27	UTSW	6	124,207,135 (GRCm39)	missense	probably benign	0.01
R4758:Vmn2r27	UTSW	6	124,208,596 (GRCm39)	missense	possibly damaging	0.87
R5018:Vmn2r27	UTSW	6	124,201,141 (GRCm39)	missense	probably benign	0.02
R5235:Vmn2r27	UTSW	6	124,169,013 (GRCm39)	missense	probably damaging	0.99
R5718:Vmn2r27	UTSW	6	124,169,103 (GRCm39)	missense	possibly damaging	0.66
R5859:Vmn2r27	UTSW	6	124,177,647 (GRCm39)	missense	probably damaging	1.00
R5958:Vmn2r27	UTSW	6	124,208,686 (GRCm39)	missense	probably benign	0.00
R6044:Vmn2r27	UTSW	6	124,208,731 (GRCm39)	missense	probably benign
R6086:Vmn2r27	UTSW	6	124,168,958 (GRCm39)	missense	probably damaging	1.00
R6396:Vmn2r27	UTSW	6	124,201,125 (GRCm39)	nonsense	probably null
R6546:Vmn2r27	UTSW	6	124,169,369 (GRCm39)	missense	possibly damaging	0.49
R6746:Vmn2r27	UTSW	6	124,177,552 (GRCm39)	missense	possibly damaging	0.47
R6976:Vmn2r27	UTSW	6	124,201,312 (GRCm39)	nonsense	probably null
R7091:Vmn2r27	UTSW	6	124,200,904 (GRCm39)	missense	possibly damaging	0.85
R7145:Vmn2r27	UTSW	6	124,168,711 (GRCm39)	missense	probably benign
R7176:Vmn2r27	UTSW	6	124,168,995 (GRCm39)	missense	probably benign	0.01
R7382:Vmn2r27	UTSW	6	124,174,276 (GRCm39)	missense	probably damaging	1.00
R7482:Vmn2r27	UTSW	6	124,201,220 (GRCm39)	missense	probably damaging	1.00
R7853:Vmn2r27	UTSW	6	124,168,980 (GRCm39)	missense	probably damaging	1.00
R7859:Vmn2r27	UTSW	6	124,201,201 (GRCm39)	missense	probably benign	0.00
R7959:Vmn2r27	UTSW	6	124,169,040 (GRCm39)	missense	probably benign
R8266:Vmn2r27	UTSW	6	124,168,937 (GRCm39)	missense	probably benign	0.00
R8353:Vmn2r27	UTSW	6	124,169,404 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r27	UTSW	6	124,168,776 (GRCm39)	missense	possibly damaging	0.71
R8463:Vmn2r27	UTSW	6	124,169,168 (GRCm39)	missense	probably damaging	1.00
R8477:Vmn2r27	UTSW	6	124,201,200 (GRCm39)	missense	probably benign	0.11
R8705:Vmn2r27	UTSW	6	124,207,188 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r27	UTSW	6	124,201,018 (GRCm39)	missense	probably benign	0.00
R9109:Vmn2r27	UTSW	6	124,174,224 (GRCm39)	missense	possibly damaging	0.95
R9140:Vmn2r27	UTSW	6	124,169,207 (GRCm39)	missense	probably damaging	1.00
R9157:Vmn2r27	UTSW	6	124,201,244 (GRCm39)	missense	probably benign	0.09
R9431:Vmn2r27	UTSW	6	124,168,856 (GRCm39)	missense	probably damaging	1.00
R9477:Vmn2r27	UTSW	6	124,168,910 (GRCm39)	missense	probably damaging	0.99
R9758:Vmn2r27	UTSW	6	124,168,637 (GRCm39)	missense	possibly damaging	0.89
Z1177:Vmn2r27	UTSW	6	124,168,860 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAGCAGCATGGACTTTGTAGCTC -3'
(R):5'- AGACATGACCAAGCACGGACTTTG -3'

Sequencing Primer
(F):5'- GCTCATAGGTGAGGTATTCATATCCC -3'
(R):5'- GACCAAGCACGGACTTTGTATTG -3'

Posted On

2014-04-24