Mutagenetix > Incidental Mutation

Incidental Mutation 'R1570:Gpr155'

177162

Institutional Source

Beutler Lab

Gene Symbol

Gpr155

Ensembl Gene

ENSMUSG00000041762

Gene Name

G protein-coupled receptor 155

Synonyms

DEPDC3, 1110017O10Rik, PGR22, F730029F15Rik

MMRRC Submission

039609-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1570 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73171850-73216842 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 73200382 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 375 (Y375C)

Ref Sequence

ENSEMBL: ENSMUSP00000107675 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076463] [ENSMUST00000112043] [ENSMUST00000112044]

AlphaFold

A2AWR3

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000048504

SMART Domains

Protein: ENSMUSP00000037772
Gene: ENSMUSG00000041762

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	1.9e-16	PFAM
Pfam:Mem_trans	187	360	4.9e-12	PFAM
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000076463
AA Change: Y375C

PolyPhen 2 Score 0.759 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: Y375C

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112043
AA Change: Y375C

PolyPhen 2 Score 0.759 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: Y375C

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112044
AA Change: Y375C

PolyPhen 2 Score 0.871 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: Y375C

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
Pfam:Mem_trans	187	360	8.3e-11	PFAM
transmembrane domain	382	404	N/A	INTRINSIC
transmembrane domain	414	436	N/A	INTRINSIC
transmembrane domain	449	471	N/A	INTRINSIC
transmembrane domain	491	510	N/A	INTRINSIC
transmembrane domain	629	651	N/A	INTRINSIC
transmembrane domain	661	683	N/A	INTRINSIC
DEP	731	805	8.28e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129184

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138573

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184503

Meta Mutation Damage Score

0.0620

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.0%

Validation Efficiency

93% (77/83)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Apobec1	A	G	6: 122,568,044 (GRCm39)		probably null	Het
Arhgap21	G	T	2: 20,885,651 (GRCm39)	Q348K	probably benign	Het
Arl5c	A	G	11: 97,883,213 (GRCm39)	V129A	probably benign	Het
Armh1	A	G	4: 117,087,189 (GRCm39)	S159P	probably damaging	Het
Asb8	A	G	15: 98,034,309 (GRCm39)	L82P	probably damaging	Het
Bahcc1	G	A	11: 120,163,009 (GRCm39)	A436T	possibly damaging	Het
Btc	T	C	5: 91,550,576 (GRCm39)	D2G	unknown	Het
C1s2	G	A	6: 124,602,723 (GRCm39)	T490M	probably benign	Het
Caap1	C	T	4: 94,444,814 (GRCm39)	G43D	probably benign	Het
Ccr5	T	C	9: 123,925,000 (GRCm39)	V201A	probably benign	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Cdhr5	C	A	7: 140,851,682 (GRCm39)	G541C	probably damaging	Het
Cep170	A	G	1: 176,583,367 (GRCm39)	I1004T	possibly damaging	Het
Chd9	A	T	8: 91,763,170 (GRCm39)	M2332L	probably benign	Het
Clk1	T	A	1: 58,453,584 (GRCm39)	H334L	probably benign	Het
Cyp4b1	G	A	4: 115,493,160 (GRCm39)	S228F	probably benign	Het
Dnah9	T	C	11: 66,003,156 (GRCm39)	N883D	probably benign	Het
Dync2h1	A	G	9: 7,176,926 (GRCm39)	L11P	probably benign	Het
Ephx4	A	G	5: 107,567,717 (GRCm39)	E225G	probably damaging	Het
Erich6	C	T	3: 58,538,080 (GRCm39)		probably null	Het
Espl1	A	G	15: 102,206,802 (GRCm39)	T89A	probably damaging	Het
Evi2	T	A	11: 79,407,076 (GRCm39)	K166N	possibly damaging	Het
Glrx5	A	G	12: 104,999,127 (GRCm39)	T57A	possibly damaging	Het
Gnptab	T	A	10: 88,255,316 (GRCm39)	V222E	probably damaging	Het
Hsd11b1	C	G	1: 192,922,635 (GRCm39)	E141Q	probably damaging	Het
Ildr2	T	C	1: 166,131,154 (GRCm39)	F337L	probably damaging	Het
Ino80	C	T	2: 119,277,509 (GRCm39)	R322Q	possibly damaging	Het
Lcp2	A	G	11: 34,039,601 (GRCm39)	D467G	probably benign	Het
Lmbr1	A	G	5: 29,459,556 (GRCm39)	I229T	probably damaging	Het
Lnpep	A	T	17: 17,799,418 (GRCm39)	M79K	probably damaging	Het
Lpin1	T	C	12: 16,610,999 (GRCm39)	Q564R	possibly damaging	Het
Lpin2	T	A	17: 71,552,176 (GRCm39)	L794*	probably null	Het
Lrrc37	A	G	11: 103,500,764 (GRCm39)	Y597H	possibly damaging	Het
Lrrc45	T	C	11: 120,610,935 (GRCm39)		probably null	Het
Mtus1	A	G	8: 41,529,278 (GRCm39)	S751P	probably damaging	Het
Nbr1	T	C	11: 101,455,656 (GRCm39)		probably benign	Het
Nup107	A	G	10: 117,599,749 (GRCm39)	F592S	possibly damaging	Het
Nup133	T	A	8: 124,675,915 (GRCm39)	M1L	possibly damaging	Het
Or2a20	T	C	6: 43,194,285 (GRCm39)	V146A	probably benign	Het
Or3a10	A	C	11: 73,935,633 (GRCm39)	F156V	possibly damaging	Het
Or4n4	T	G	14: 50,518,981 (GRCm39)	H243P	probably damaging	Het
Or4p8	A	C	2: 88,727,290 (GRCm39)	I217S	probably damaging	Het
Or52b4i	C	A	7: 102,191,177 (GRCm39)	H11Q	probably damaging	Het
Or8i2	A	T	2: 86,852,616 (GRCm39)	S91T	probably benign	Het
Otud7b	T	C	3: 96,063,208 (GRCm39)	C816R	probably damaging	Het
Pi4k2a	T	C	19: 42,089,083 (GRCm39)	V148A	probably benign	Het
Pih1d2	T	C	9: 50,532,479 (GRCm39)	M195T	probably benign	Het
Pira13	T	C	7: 3,826,060 (GRCm39)	E311G	probably benign	Het
Plpp6	T	C	19: 28,942,178 (GRCm39)	F260L	probably damaging	Het
R3hcc1l	A	T	19: 42,570,393 (GRCm39)	T663S	probably damaging	Het
Rnf25	T	C	1: 74,634,426 (GRCm39)	E199G	probably damaging	Het
Scin	G	A	12: 40,134,380 (GRCm39)		probably benign	Het
Serpinb1c	A	T	13: 33,080,973 (GRCm39)	S37T	probably benign	Het
Snx19	A	G	9: 30,339,639 (GRCm39)	D259G	probably damaging	Het
Sorcs3	A	G	19: 48,752,620 (GRCm39)	K805R	probably damaging	Het
Sox6	C	A	7: 115,376,358 (GRCm39)	G125W	probably damaging	Het
Spink5	A	G	18: 44,100,174 (GRCm39)	I64V	probably benign	Het
Spmip10	T	A	18: 56,727,606 (GRCm39)	D101E	probably benign	Het
St6galnac1	A	G	11: 116,657,474 (GRCm39)		probably benign	Het
Sult1c2	T	C	17: 54,143,991 (GRCm39)	I105V	probably benign	Het
Tacr3	T	G	3: 134,535,517 (GRCm39)	S162A	probably damaging	Het
Ttc6	T	C	12: 57,721,549 (GRCm39)	S1013P	probably damaging	Het
Zbtb11	C	A	16: 55,811,178 (GRCm39)	N445K	probably benign	Het
Zfp423	A	C	8: 88,509,186 (GRCm39)	V261G	probably benign	Het
Zfp59	T	C	7: 27,553,016 (GRCm39)	V156A	probably benign	Het
Zscan2	C	A	7: 80,513,141 (GRCm39)	A42E	probably damaging	Het

Other mutations in Gpr155

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00731:Gpr155	APN	2	73,192,957 (GRCm39)	missense	probably damaging	1.00
IGL01432:Gpr155	APN	2	73,182,229 (GRCm39)	missense	possibly damaging	0.51
IGL01528:Gpr155	APN	2	73,192,767 (GRCm39)	critical splice donor site	probably null
IGL01718:Gpr155	APN	2	73,212,576 (GRCm39)	missense	probably benign
IGL01733:Gpr155	APN	2	73,183,956 (GRCm39)	splice site	probably null
IGL03342:Gpr155	APN	2	73,180,022 (GRCm39)	missense	probably damaging	1.00
PIT1430001:Gpr155	UTSW	2	73,200,482 (GRCm39)	missense	probably benign	0.08
PIT4810001:Gpr155	UTSW	2	73,178,607 (GRCm39)	missense	probably benign	0.08
R0226:Gpr155	UTSW	2	73,197,936 (GRCm39)	missense	probably benign	0.02
R0399:Gpr155	UTSW	2	73,200,346 (GRCm39)	missense	possibly damaging	0.91
R0445:Gpr155	UTSW	2	73,200,488 (GRCm39)	splice site	probably benign
R1598:Gpr155	UTSW	2	73,200,434 (GRCm39)	missense	probably damaging	0.98
R1647:Gpr155	UTSW	2	73,194,508 (GRCm39)	splice site	probably null
R1648:Gpr155	UTSW	2	73,194,508 (GRCm39)	splice site	probably null
R1756:Gpr155	UTSW	2	73,197,921 (GRCm39)	missense	probably benign	0.00
R1760:Gpr155	UTSW	2	73,212,279 (GRCm39)	missense	probably damaging	1.00
R2033:Gpr155	UTSW	2	73,178,526 (GRCm39)	missense	probably benign	0.18
R2044:Gpr155	UTSW	2	73,203,977 (GRCm39)	missense	probably damaging	1.00
R2145:Gpr155	UTSW	2	73,187,002 (GRCm39)	missense	probably benign	0.01
R2172:Gpr155	UTSW	2	73,212,471 (GRCm39)	missense	probably benign	0.00
R2274:Gpr155	UTSW	2	73,178,479 (GRCm39)	critical splice donor site	probably null
R3878:Gpr155	UTSW	2	73,198,736 (GRCm39)	nonsense	probably null
R3924:Gpr155	UTSW	2	73,200,420 (GRCm39)	missense	probably damaging	1.00
R4910:Gpr155	UTSW	2	73,197,882 (GRCm39)	nonsense	probably null
R4950:Gpr155	UTSW	2	73,212,529 (GRCm39)	missense	probably benign
R5337:Gpr155	UTSW	2	73,178,592 (GRCm39)	missense	probably benign	0.32
R5830:Gpr155	UTSW	2	73,200,433 (GRCm39)	missense	possibly damaging	0.93
R5887:Gpr155	UTSW	2	73,174,062 (GRCm39)	nonsense	probably null
R5929:Gpr155	UTSW	2	73,204,011 (GRCm39)	nonsense	probably null
R6293:Gpr155	UTSW	2	73,204,341 (GRCm39)	missense	possibly damaging	0.47
R6553:Gpr155	UTSW	2	73,179,989 (GRCm39)	missense	probably damaging	1.00
R6585:Gpr155	UTSW	2	73,179,989 (GRCm39)	missense	probably damaging	1.00
R7003:Gpr155	UTSW	2	73,173,961 (GRCm39)	missense	probably damaging	0.99
R7353:Gpr155	UTSW	2	73,197,835 (GRCm39)	nonsense	probably null
R7506:Gpr155	UTSW	2	73,198,683 (GRCm39)	missense	probably damaging	0.97
R7631:Gpr155	UTSW	2	73,213,291 (GRCm39)	intron	probably benign
R7753:Gpr155	UTSW	2	73,212,550 (GRCm39)	missense	probably benign	0.27
R7810:Gpr155	UTSW	2	73,212,296 (GRCm39)	missense	probably damaging	0.99
R7813:Gpr155	UTSW	2	73,212,329 (GRCm39)	nonsense	probably null
R7815:Gpr155	UTSW	2	73,192,904 (GRCm39)	missense	probably benign
R7873:Gpr155	UTSW	2	73,173,934 (GRCm39)	missense	possibly damaging	0.51
R8506:Gpr155	UTSW	2	73,173,806 (GRCm39)	missense	probably damaging	0.99
R8680:Gpr155	UTSW	2	73,174,039 (GRCm39)	missense	probably damaging	0.99
R8856:Gpr155	UTSW	2	73,203,993 (GRCm39)	missense	probably benign	0.27
R8872:Gpr155	UTSW	2	73,197,936 (GRCm39)	missense	probably benign	0.02
R9116:Gpr155	UTSW	2	73,204,109 (GRCm39)	missense	possibly damaging	0.78
R9683:Gpr155	UTSW	2	73,192,780 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GCTCAAAACTCCTGACTGGAAAGACAT -3'
(R):5'- GGGGCCAACAGAGCATTCTCTTATAAC -3'

Sequencing Primer
(F):5'- CTGGAAAGACATACTCAGTCAATTAC -3'
(R):5'- CGGATAATGTGCCGGATTCC -3'

Posted On

2014-04-24