Mutagenetix > Incidental Mutation

Incidental Mutation 'R1584:Tln2'

177351

Institutional Source

Beutler Lab

Gene Symbol

Tln2

Ensembl Gene

ENSMUSG00000052698

Gene Name

talin 2

Synonyms

MMRRC Submission

039621-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.216) question?

Stock #

R1584 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67217087-67559703 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 67296414 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 470 (N470I)

Ref Sequence

ENSEMBL: ENSMUSP00000149474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039662] [ENSMUST00000040025] [ENSMUST00000215267] [ENSMUST00000215784] [ENSMUST00000217550]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000039662
AA Change: N1556I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000035272
Gene: ENSMUSG00000052698
AA Change: N1556I

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	3.4e-59	PFAM
Pfam:I_LWEQ	661	765	1.9e-10	PFAM
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	2.6e-67	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2384	2531	2.5e-52	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000040025
AA Change: N1556I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000039633
Gene: ENSMUSG00000052698
AA Change: N1556I

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	8.5e-78	PFAM
low complexity region	674	693	N/A	INTRINSIC
internal_repeat_2	703	763	2.05e-7	PROSPERO
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	9.9e-72	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2383	2533	4.3e-51	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000214859
AA Change: N10I

Predicted Effect

probably damaging
Transcript: ENSMUST00000215267
AA Change: N466I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215784
AA Change: N1558I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000217550
AA Change: N470I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.2256

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.2%

Validation Efficiency

99% (106/107)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal muscle morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd15	C	T	11: 77,515,410 (GRCm38)	A71V	probably damaging	Het
Ager	A	G	17: 34,600,718 (GRCm38)	E357G	probably damaging	Het
Akap13	T	C	7: 75,729,049 (GRCm38)	S2095P	possibly damaging	Het
Ampd2	T	C	3: 108,080,337 (GRCm38)		probably null	Het
Arrdc1	T	C	2: 24,925,795 (GRCm38)	I398V	probably benign	Het
Ash1l	T	C	3: 89,052,065 (GRCm38)	Y2250H	probably damaging	Het
Brca2	G	A	5: 150,552,258 (GRCm38)	A2478T	probably damaging	Het
Btrc	T	A	19: 45,513,382 (GRCm38)		probably benign	Het
C8g	C	T	2: 25,500,216 (GRCm38)	A6T	probably benign	Het
Ccdc121rt3	C	T	5: 112,354,764 (GRCm38)	G358D	probably benign	Het
Cdc123	T	A	2: 5,803,977 (GRCm38)		probably null	Het
Cilp	G	A	9: 65,279,715 (GRCm38)	G1031S	probably damaging	Het
Cldn5	G	A	16: 18,777,477 (GRCm38)	G161D	probably damaging	Het
Cndp2	G	A	18: 84,677,315 (GRCm38)		probably benign	Het
Cntnap1	C	A	11: 101,180,360 (GRCm38)	F366L	probably damaging	Het
Corin	C	T	5: 72,302,790 (GRCm38)		probably null	Het
Ctdspl2	G	A	2: 122,003,929 (GRCm38)	R332K	probably benign	Het
Dido1	G	A	2: 180,662,328 (GRCm38)	P1261L	probably damaging	Het
Dop1a	A	T	9: 86,548,172 (GRCm38)	R2200*	probably null	Het
Dtx3l	G	A	16: 35,932,728 (GRCm38)	L503F	probably damaging	Het
Dysf	A	G	6: 84,067,047 (GRCm38)	K259R	probably benign	Het
Enpep	T	A	3: 129,319,448 (GRCm38)	T203S	probably damaging	Het
Epha2	T	A	4: 141,322,047 (GRCm38)		probably null	Het
Fam20b	T	C	1: 156,686,188 (GRCm38)		probably benign	Het
Fbln7	A	G	2: 128,877,429 (GRCm38)	T49A	probably benign	Het
Fgf14	T	A	14: 124,676,539 (GRCm38)	K60M	probably benign	Het
Fhad1	T	C	4: 141,985,511 (GRCm38)	I206V	probably benign	Het
Figla	A	G	6: 86,020,782 (GRCm38)	E164G	probably benign	Het
Gimap4	C	A	6: 48,691,282 (GRCm38)	Q196K	probably benign	Het
Glcci1	C	T	6: 8,537,964 (GRCm38)	T6I	probably damaging	Het
Grk4	T	C	5: 34,694,750 (GRCm38)	S113P	probably benign	Het
Hectd3	G	A	4: 116,996,566 (GRCm38)	E220K	probably damaging	Het
Hecw1	A	G	13: 14,340,743 (GRCm38)		probably null	Het
Helz2	G	A	2: 181,236,297 (GRCm38)	P903S	probably damaging	Het
Hydin	T	C	8: 110,580,815 (GRCm38)	V3942A	probably benign	Het
Irs1	G	T	1: 82,289,444 (GRCm38)	H350Q	probably benign	Het
Kdm1b	G	A	13: 47,064,054 (GRCm38)	E46K	probably damaging	Het
Klf11	A	G	12: 24,655,305 (GRCm38)	N253D	probably damaging	Het
Klhl23	T	C	2: 69,833,888 (GRCm38)	I527T	probably damaging	Het
Lars1	G	A	18: 42,210,050 (GRCm38)	R1101C	probably damaging	Het
Lcn4	G	A	2: 26,668,576 (GRCm38)	P166L	probably damaging	Het
Letmd1	T	A	15: 100,472,542 (GRCm38)		probably null	Het
Lilra6	T	A	7: 3,912,662 (GRCm38)	D358V	probably damaging	Het
Mmrn2	A	G	14: 34,375,685 (GRCm38)	D24G	probably benign	Het
Mroh2b	G	T	15: 4,925,684 (GRCm38)	D720Y	probably damaging	Het
Mrps35	C	T	6: 147,055,984 (GRCm38)	T169M	probably damaging	Het
Muc4	G	A	16: 32,753,595 (GRCm38)	G1157D	probably benign	Het
Naga	T	A	15: 82,334,788 (GRCm38)	M237L	probably null	Het
Nfu1	G	A	6: 87,020,809 (GRCm38)	E225K	probably damaging	Het
Nin	A	T	12: 70,042,669 (GRCm38)	L1324Q	probably benign	Het
Oc90	T	A	15: 65,897,720 (GRCm38)	Y96F	probably damaging	Het
Or4a80	C	T	2: 89,752,267 (GRCm38)	C187Y	probably damaging	Het
Or5b116	T	C	19: 13,445,659 (GRCm38)	Y216H	probably damaging	Het
Or5m11b	T	A	2: 85,975,995 (GRCm38)	F251I	probably damaging	Het
Or6ae1	A	G	7: 140,162,203 (GRCm38)	V249A	probably damaging	Het
Or8c9	G	A	9: 38,330,131 (GRCm38)	M181I	possibly damaging	Het
Orc4	A	T	2: 48,909,494 (GRCm38)	C324S	possibly damaging	Het
Pals1	A	G	12: 78,829,727 (GRCm38)	I482V	probably benign	Het
Pdzrn4	T	C	15: 92,770,537 (GRCm38)	S857P	probably benign	Het
Plec	C	T	15: 76,185,908 (GRCm38)	E1000K	possibly damaging	Het
Plvap	A	T	8: 71,508,481 (GRCm38)	V149D	probably benign	Het
Pm20d2	A	T	4: 33,174,772 (GRCm38)	N371K	probably damaging	Het
Ppp2r5d	A	G	17: 46,684,684 (GRCm38)	Y480H	probably benign	Het
Prkd1	A	T	12: 50,425,515 (GRCm38)	V205E	probably damaging	Het
R3hdm2	A	G	10: 127,476,690 (GRCm38)	I434V	probably benign	Het
Rel	T	A	11: 23,745,546 (GRCm38)	T246S	probably damaging	Het
Rnf215	T	A	11: 4,136,719 (GRCm38)	V172E	probably damaging	Het
Scara3	T	C	14: 65,921,104 (GRCm38)	D485G	probably damaging	Het
Sec16a	A	T	2: 26,431,157 (GRCm38)	Y1308N	probably damaging	Het
Sis	A	T	3: 72,932,060 (GRCm38)	D824E	possibly damaging	Het
Slc27a4	T	A	2: 29,811,190 (GRCm38)	V331E	probably damaging	Het
Srebf1	C	T	11: 60,200,702 (GRCm38)	R999H	probably benign	Het
St3gal3	A	C	4: 118,107,662 (GRCm38)	M1R	probably null	Het
Tapbp	C	T	17: 33,919,940 (GRCm38)		probably null	Het
Tep1	A	T	14: 50,866,037 (GRCm38)	N265K	probably damaging	Het
Thoc2l	T	A	5: 104,518,257 (GRCm38)	I215N	probably damaging	Het
Trim43a	G	T	9: 88,588,158 (GRCm38)	W339L	probably damaging	Het
Ttc22	T	C	4: 106,622,780 (GRCm38)	F77S	probably damaging	Het
Ttc8	A	G	12: 98,920,764 (GRCm38)	E32G	probably benign	Het
Uhmk1	A	T	1: 170,208,653 (GRCm38)		probably null	Het
Usp17lc	A	G	7: 103,418,941 (GRCm38)	H481R	possibly damaging	Het
Vamp8	G	A	6: 72,385,634 (GRCm38)	T35M	probably damaging	Het
Vill	A	G	9: 119,065,586 (GRCm38)	Y53C	probably damaging	Het
Vmn1r222	A	T	13: 23,232,762 (GRCm38)	S94T	probably damaging	Het
Vmn2r93	A	G	17: 18,305,151 (GRCm38)	Y357C	possibly damaging	Het
Vps13c	T	A	9: 67,893,112 (GRCm38)	V536D	possibly damaging	Het
Vrtn	G	A	12: 84,650,081 (GRCm38)	C535Y	probably damaging	Het
Vwa3a	A	G	7: 120,768,165 (GRCm38)	Y181C	probably damaging	Het
Wfikkn2	G	A	11: 94,238,895 (GRCm38)	T140I	probably damaging	Het
Ykt6	T	A	11: 5,962,349 (GRCm38)	F101I	probably damaging	Het
Zfp277	C	T	12: 40,378,826 (GRCm38)	G174D	probably benign	Het
Zfp638	A	G	6: 83,978,065 (GRCm38)		probably null	Het
Zzef1	C	T	11: 72,924,679 (GRCm38)	P2942S	probably damaging	Het

Other mutations in Tln2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00468:Tln2	APN	9	67,344,187 (GRCm38)	missense	possibly damaging	0.59
IGL01110:Tln2	APN	9	67,250,582 (GRCm38)	nonsense	probably null
IGL01112:Tln2	APN	9	67,311,811 (GRCm38)	missense	probably damaging	1.00
IGL01307:Tln2	APN	9	67,395,467 (GRCm38)	missense	probably benign	0.25
IGL01374:Tln2	APN	9	67,261,923 (GRCm38)	missense	probably damaging	1.00
IGL01625:Tln2	APN	9	67,370,623 (GRCm38)	missense	probably damaging	1.00
IGL01865:Tln2	APN	9	67,250,614 (GRCm38)	nonsense	probably null
IGL01999:Tln2	APN	9	67,392,505 (GRCm38)	missense	possibly damaging	0.81
IGL02002:Tln2	APN	9	67,356,698 (GRCm38)	missense	probably damaging	0.98
IGL02005:Tln2	APN	9	67,392,505 (GRCm38)	missense	possibly damaging	0.81
IGL02015:Tln2	APN	9	67,361,439 (GRCm38)	splice site	probably benign
IGL02368:Tln2	APN	9	67,240,810 (GRCm38)	splice site	probably benign
IGL02444:Tln2	APN	9	67,258,592 (GRCm38)	splice site	probably benign
IGL02646:Tln2	APN	9	67,255,996 (GRCm38)	missense	probably benign	0.43
IGL02744:Tln2	APN	9	67,229,376 (GRCm38)	nonsense	probably null
IGL02869:Tln2	APN	9	67,221,525 (GRCm38)	splice site	probably benign
IGL02930:Tln2	APN	9	67,393,662 (GRCm38)	nonsense	probably null
IGL03100:Tln2	APN	9	67,295,737 (GRCm38)	missense	probably damaging	1.00
IGL03326:Tln2	APN	9	67,334,257 (GRCm38)	missense	possibly damaging	0.67
Harrier	UTSW	9	67,330,552 (GRCm38)	nonsense	probably null
Marsh	UTSW	9	67,272,654 (GRCm38)	missense	probably benign	0.19
BB008:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
BB018:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
R0047:Tln2	UTSW	9	67,240,672 (GRCm38)	splice site	probably benign
R0047:Tln2	UTSW	9	67,240,672 (GRCm38)	splice site	probably benign
R0107:Tln2	UTSW	9	67,370,706 (GRCm38)	missense	probably damaging	1.00
R0494:Tln2	UTSW	9	67,355,197 (GRCm38)	missense	probably benign	0.22
R0884:Tln2	UTSW	9	67,370,733 (GRCm38)	missense	probably damaging	1.00
R0947:Tln2	UTSW	9	67,295,813 (GRCm38)	missense	probably benign	0.08
R0989:Tln2	UTSW	9	67,229,454 (GRCm38)	missense	probably damaging	1.00
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1486:Tln2	UTSW	9	67,311,839 (GRCm38)	missense	probably damaging	1.00
R1527:Tln2	UTSW	9	67,272,668 (GRCm38)	missense	possibly damaging	0.95
R1636:Tln2	UTSW	9	67,306,532 (GRCm38)	missense	probably damaging	1.00
R1656:Tln2	UTSW	9	67,227,107 (GRCm38)	missense	possibly damaging	0.81
R1707:Tln2	UTSW	9	67,375,807 (GRCm38)	missense	probably benign	0.00
R1749:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1751:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1761:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1767:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1815:Tln2	UTSW	9	67,229,423 (GRCm38)	missense	probably damaging	1.00
R1840:Tln2	UTSW	9	67,342,043 (GRCm38)	missense	probably damaging	1.00
R1847:Tln2	UTSW	9	67,362,687 (GRCm38)	nonsense	probably null
R1964:Tln2	UTSW	9	67,342,135 (GRCm38)	missense	probably benign	0.00
R1968:Tln2	UTSW	9	67,255,901 (GRCm38)	missense	probably damaging	1.00
R2036:Tln2	UTSW	9	67,272,704 (GRCm38)	missense	possibly damaging	0.76
R2038:Tln2	UTSW	9	67,397,653 (GRCm38)	start codon destroyed	probably benign	0.01
R2152:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2153:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2154:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2191:Tln2	UTSW	9	67,355,221 (GRCm38)	missense	probably damaging	1.00
R2192:Tln2	UTSW	9	67,355,221 (GRCm38)	missense	probably damaging	1.00
R2201:Tln2	UTSW	9	67,375,757 (GRCm38)	missense	probably damaging	1.00
R3116:Tln2	UTSW	9	67,355,139 (GRCm38)	missense	probably benign	0.10
R3151:Tln2	UTSW	9	67,330,547 (GRCm38)	critical splice donor site	probably null
R3795:Tln2	UTSW	9	67,255,915 (GRCm38)	missense	probably damaging	0.97
R3953:Tln2	UTSW	9	67,370,629 (GRCm38)	missense	probably damaging	1.00
R4450:Tln2	UTSW	9	67,344,065 (GRCm38)	critical splice donor site	probably null
R4685:Tln2	UTSW	9	67,302,572 (GRCm38)	missense	probably damaging	1.00
R4688:Tln2	UTSW	9	67,397,653 (GRCm38)	start codon destroyed	probably benign	0.01
R4696:Tln2	UTSW	9	67,395,461 (GRCm38)	missense	probably damaging	1.00
R4697:Tln2	UTSW	9	67,395,461 (GRCm38)	missense	probably damaging	1.00
R4700:Tln2	UTSW	9	67,346,527 (GRCm38)	missense	probably benign	0.03
R4701:Tln2	UTSW	9	67,346,527 (GRCm38)	missense	probably benign	0.03
R4741:Tln2	UTSW	9	67,386,555 (GRCm38)	critical splice donor site	probably null
R4806:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4807:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4808:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4967:Tln2	UTSW	9	67,355,125 (GRCm38)	missense	probably damaging	0.97
R5061:Tln2	UTSW	9	67,354,468 (GRCm38)	missense	probably benign
R5092:Tln2	UTSW	9	67,256,028 (GRCm38)	missense	probably benign	0.13
R5093:Tln2	UTSW	9	67,334,314 (GRCm38)	missense	probably benign	0.44
R5126:Tln2	UTSW	9	67,258,535 (GRCm38)	missense	probably damaging	1.00
R5204:Tln2	UTSW	9	67,354,482 (GRCm38)	missense	probably benign	0.00
R5236:Tln2	UTSW	9	67,365,923 (GRCm38)	missense	probably damaging	0.99
R5287:Tln2	UTSW	9	67,242,359 (GRCm38)	missense	probably damaging	1.00
R5568:Tln2	UTSW	9	67,311,865 (GRCm38)	missense	probably damaging	1.00
R5571:Tln2	UTSW	9	67,334,320 (GRCm38)	missense	possibly damaging	0.88
R5642:Tln2	UTSW	9	67,296,358 (GRCm38)	missense	probably benign	0.01
R5711:Tln2	UTSW	9	67,392,547 (GRCm38)	missense	probably benign	0.00
R5776:Tln2	UTSW	9	67,258,250 (GRCm38)	missense	probably damaging	1.00
R5791:Tln2	UTSW	9	67,386,605 (GRCm38)	missense	probably damaging	0.98
R5866:Tln2	UTSW	9	67,266,868 (GRCm38)	missense	probably damaging	1.00
R5888:Tln2	UTSW	9	67,229,403 (GRCm38)	missense	probably damaging	1.00
R5902:Tln2	UTSW	9	67,362,717 (GRCm38)	missense	probably benign	0.02
R6106:Tln2	UTSW	9	67,323,020 (GRCm38)	missense	probably damaging	0.99
R6175:Tln2	UTSW	9	67,224,081 (GRCm38)	missense	probably damaging	1.00
R6385:Tln2	UTSW	9	67,278,129 (GRCm38)	missense	probably benign	0.45
R6430:Tln2	UTSW	9	67,272,665 (GRCm38)	missense	probably damaging	1.00
R6441:Tln2	UTSW	9	67,272,689 (GRCm38)	missense	probably damaging	1.00
R6738:Tln2	UTSW	9	67,386,664 (GRCm38)	missense	possibly damaging	0.91
R6776:Tln2	UTSW	9	67,262,905 (GRCm38)	missense	probably damaging	1.00
R6794:Tln2	UTSW	9	67,286,558 (GRCm38)	missense	probably benign	0.07
R6850:Tln2	UTSW	9	67,258,535 (GRCm38)	missense	probably damaging	1.00
R6907:Tln2	UTSW	9	67,397,635 (GRCm38)	missense	probably damaging	0.98
R6909:Tln2	UTSW	9	67,392,532 (GRCm38)	missense	probably damaging	0.97
R6951:Tln2	UTSW	9	67,258,485 (GRCm38)	missense	probably damaging	0.97
R7015:Tln2	UTSW	9	67,362,647 (GRCm38)	missense	possibly damaging	0.55
R7051:Tln2	UTSW	9	67,346,417 (GRCm38)	missense	probably benign	0.00
R7246:Tln2	UTSW	9	67,262,979 (GRCm38)	missense	probably damaging	1.00
R7292:Tln2	UTSW	9	67,346,461 (GRCm38)	missense	probably benign
R7753:Tln2	UTSW	9	67,395,473 (GRCm38)	missense	probably damaging	1.00
R7868:Tln2	UTSW	9	67,348,226 (GRCm38)	missense	probably damaging	1.00
R7931:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
R8023:Tln2	UTSW	9	67,224,064 (GRCm38)	missense	probably damaging	1.00
R8081:Tln2	UTSW	9	67,356,747 (GRCm38)	missense	probably damaging	1.00
R8164:Tln2	UTSW	9	67,319,420 (GRCm38)	missense	probably benign	0.31
R8192:Tln2	UTSW	9	67,346,529 (GRCm38)	nonsense	probably null
R8495:Tln2	UTSW	9	67,354,467 (GRCm38)	missense	probably benign	0.01
R8734:Tln2	UTSW	9	67,272,654 (GRCm38)	missense	probably benign	0.19
R8739:Tln2	UTSW	9	67,258,273 (GRCm38)	missense	probably damaging	1.00
R8757:Tln2	UTSW	9	67,367,218 (GRCm38)	missense	probably damaging	1.00
R8759:Tln2	UTSW	9	67,367,218 (GRCm38)	missense	probably damaging	1.00
R8770:Tln2	UTSW	9	67,323,022 (GRCm38)	missense	probably benign
R8781:Tln2	UTSW	9	67,255,951 (GRCm38)	missense	probably damaging	1.00
R8812:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8814:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8816:Tln2	UTSW	9	67,221,517 (GRCm38)	missense	probably damaging	1.00
R8816:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8833:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8835:Tln2	UTSW	9	67,397,693 (GRCm38)	splice site	probably benign
R8837:Tln2	UTSW	9	67,250,584 (GRCm38)	missense	probably damaging	0.99
R8843:Tln2	UTSW	9	67,395,545 (GRCm38)	missense	probably damaging	1.00
R8864:Tln2	UTSW	9	67,330,552 (GRCm38)	nonsense	probably null
R8867:Tln2	UTSW	9	67,330,550 (GRCm38)	missense	probably damaging	0.98
R8921:Tln2	UTSW	9	67,266,823 (GRCm38)	missense	probably damaging	0.99
R9080:Tln2	UTSW	9	67,346,561 (GRCm38)	missense	probably damaging	1.00
R9083:Tln2	UTSW	9	67,362,645 (GRCm38)	missense	probably damaging	0.96
R9150:Tln2	UTSW	9	67,221,496 (GRCm38)	missense	probably damaging	1.00
R9287:Tln2	UTSW	9	67,370,698 (GRCm38)	missense	probably benign	0.20
R9330:Tln2	UTSW	9	67,321,931 (GRCm38)	missense	possibly damaging	0.61
R9343:Tln2	UTSW	9	67,323,071 (GRCm38)	missense	probably benign	0.10
R9355:Tln2	UTSW	9	67,355,247 (GRCm38)	missense	possibly damaging	0.46
R9383:Tln2	UTSW	9	67,370,761 (GRCm38)	missense	probably benign	0.17
R9386:Tln2	UTSW	9	67,365,967 (GRCm38)	missense	possibly damaging	0.78
R9407:Tln2	UTSW	9	67,229,450 (GRCm38)	missense	probably damaging	1.00
R9483:Tln2	UTSW	9	67,392,487 (GRCm38)	missense	probably damaging	1.00
R9523:Tln2	UTSW	9	67,258,484 (GRCm38)	missense	probably damaging	0.99
R9642:Tln2	UTSW	9	67,250,544 (GRCm38)	missense	probably benign	0.02
R9703:Tln2	UTSW	9	67,386,656 (GRCm38)	missense	probably damaging	1.00
X0027:Tln2	UTSW	9	67,376,853 (GRCm38)	missense	probably damaging	1.00
X0064:Tln2	UTSW	9	67,348,138 (GRCm38)	missense	probably damaging	1.00
X0067:Tln2	UTSW	9	67,370,691 (GRCm38)	missense	probably damaging	1.00
Z1176:Tln2	UTSW	9	67,346,485 (GRCm38)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- CCAGTCCCAGGCACAAAGATTATTCTC -3'
(R):5'- TCATTTGGACGGAAATGGACTTACTCC -3'

Sequencing Primer
(F):5'- GTACCATCCACAGTAACAGAGTTATG -3'
(R):5'- TCATGACAGCATGGCTGG -3'

Posted On

2014-04-24