Mutagenetix > Incidental Mutation

Incidental Mutation 'R1584:Tln2'

177351

Institutional Source

Beutler Lab

Gene Symbol

Tln2

Ensembl Gene

ENSMUSG00000052698

Gene Name

talin 2

Synonyms

MMRRC Submission

039621-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.185) question?

Stock #

R1584 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67124369-67466985 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 67203696 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 470 (N470I)

Ref Sequence

ENSEMBL: ENSMUSP00000149474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039662] [ENSMUST00000040025] [ENSMUST00000215267] [ENSMUST00000215784] [ENSMUST00000217550]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000039662
AA Change: N1556I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000035272
Gene: ENSMUSG00000052698
AA Change: N1556I

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	3.4e-59	PFAM
Pfam:I_LWEQ	661	765	1.9e-10	PFAM
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	2.6e-67	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2384	2531	2.5e-52	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000040025
AA Change: N1556I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000039633
Gene: ENSMUSG00000052698
AA Change: N1556I

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	8.5e-78	PFAM
low complexity region	674	693	N/A	INTRINSIC
internal_repeat_2	703	763	2.05e-7	PROSPERO
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	9.9e-72	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2383	2533	4.3e-51	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000214859
AA Change: N10I

Predicted Effect

probably damaging
Transcript: ENSMUST00000215267
AA Change: N466I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215784
AA Change: N1558I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000217550
AA Change: N470I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.2256

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.6%
20x: 90.2%

Validation Efficiency

99% (106/107)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal muscle morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd15	C	T	11: 77,406,236 (GRCm39)	A71V	probably damaging	Het
Ager	A	G	17: 34,819,692 (GRCm39)	E357G	probably damaging	Het
Akap13	T	C	7: 75,378,797 (GRCm39)	S2095P	possibly damaging	Het
Ampd2	T	C	3: 107,987,653 (GRCm39)		probably null	Het
Arrdc1	T	C	2: 24,815,807 (GRCm39)	I398V	probably benign	Het
Ash1l	T	C	3: 88,959,372 (GRCm39)	Y2250H	probably damaging	Het
Brca2	G	A	5: 150,475,723 (GRCm39)	A2478T	probably damaging	Het
Btrc	T	A	19: 45,501,821 (GRCm39)		probably benign	Het
C8g	C	T	2: 25,390,228 (GRCm39)	A6T	probably benign	Het
Ccdc121rt3	C	T	5: 112,502,630 (GRCm39)	G358D	probably benign	Het
Cdc123	T	A	2: 5,808,788 (GRCm39)		probably null	Het
Cilp	G	A	9: 65,186,997 (GRCm39)	G1031S	probably damaging	Het
Cldn5	G	A	16: 18,596,227 (GRCm39)	G161D	probably damaging	Het
Cndp2	G	A	18: 84,695,440 (GRCm39)		probably benign	Het
Cntnap1	C	A	11: 101,071,186 (GRCm39)	F366L	probably damaging	Het
Corin	C	T	5: 72,460,133 (GRCm39)		probably null	Het
Ctdspl2	G	A	2: 121,834,410 (GRCm39)	R332K	probably benign	Het
Dido1	G	A	2: 180,304,121 (GRCm39)	P1261L	probably damaging	Het
Dop1a	A	T	9: 86,430,225 (GRCm39)	R2200*	probably null	Het
Dtx3l	G	A	16: 35,753,098 (GRCm39)	L503F	probably damaging	Het
Dysf	A	G	6: 84,044,029 (GRCm39)	K259R	probably benign	Het
Enpep	T	A	3: 129,113,097 (GRCm39)	T203S	probably damaging	Het
Epha2	T	A	4: 141,049,358 (GRCm39)		probably null	Het
Fam20b	T	C	1: 156,513,758 (GRCm39)		probably benign	Het
Fbln7	A	G	2: 128,719,349 (GRCm39)	T49A	probably benign	Het
Fgf14	T	A	14: 124,913,951 (GRCm39)	K60M	probably benign	Het
Fhad1	T	C	4: 141,712,822 (GRCm39)	I206V	probably benign	Het
Figla	A	G	6: 85,997,764 (GRCm39)	E164G	probably benign	Het
Gimap4	C	A	6: 48,668,216 (GRCm39)	Q196K	probably benign	Het
Glcci1	C	T	6: 8,537,964 (GRCm39)	T6I	probably damaging	Het
Grk4	T	C	5: 34,852,094 (GRCm39)	S113P	probably benign	Het
Hectd3	G	A	4: 116,853,763 (GRCm39)	E220K	probably damaging	Het
Hecw1	A	G	13: 14,515,328 (GRCm39)		probably null	Het
Helz2	G	A	2: 180,878,090 (GRCm39)	P903S	probably damaging	Het
Hydin	T	C	8: 111,307,447 (GRCm39)	V3942A	probably benign	Het
Irs1	G	T	1: 82,267,165 (GRCm39)	H350Q	probably benign	Het
Kdm1b	G	A	13: 47,217,530 (GRCm39)	E46K	probably damaging	Het
Klf11	A	G	12: 24,705,304 (GRCm39)	N253D	probably damaging	Het
Klhl23	T	C	2: 69,664,232 (GRCm39)	I527T	probably damaging	Het
Lars1	G	A	18: 42,343,115 (GRCm39)	R1101C	probably damaging	Het
Lcn4	G	A	2: 26,558,588 (GRCm39)	P166L	probably damaging	Het
Letmd1	T	A	15: 100,370,423 (GRCm39)		probably null	Het
Lilra6	T	A	7: 3,915,661 (GRCm39)	D358V	probably damaging	Het
Mmrn2	A	G	14: 34,097,642 (GRCm39)	D24G	probably benign	Het
Mroh2b	G	T	15: 4,955,166 (GRCm39)	D720Y	probably damaging	Het
Mrps35	C	T	6: 146,957,482 (GRCm39)	T169M	probably damaging	Het
Muc4	G	A	16: 32,574,886 (GRCm39)	G1157D	probably benign	Het
Naga	T	A	15: 82,218,989 (GRCm39)	M237L	probably null	Het
Nfu1	G	A	6: 86,997,791 (GRCm39)	E225K	probably damaging	Het
Nin	A	T	12: 70,089,443 (GRCm39)	L1324Q	probably benign	Het
Oc90	T	A	15: 65,769,569 (GRCm39)	Y96F	probably damaging	Het
Or4a80	C	T	2: 89,582,611 (GRCm39)	C187Y	probably damaging	Het
Or5b116	T	C	19: 13,423,023 (GRCm39)	Y216H	probably damaging	Het
Or5m11b	T	A	2: 85,806,339 (GRCm39)	F251I	probably damaging	Het
Or6ae1	A	G	7: 139,742,116 (GRCm39)	V249A	probably damaging	Het
Or8c9	G	A	9: 38,241,427 (GRCm39)	M181I	possibly damaging	Het
Orc4	A	T	2: 48,799,506 (GRCm39)	C324S	possibly damaging	Het
Pals1	A	G	12: 78,876,501 (GRCm39)	I482V	probably benign	Het
Pdzrn4	T	C	15: 92,668,418 (GRCm39)	S857P	probably benign	Het
Plec	C	T	15: 76,070,108 (GRCm39)	E1000K	possibly damaging	Het
Plvap	A	T	8: 71,961,125 (GRCm39)	V149D	probably benign	Het
Pm20d2	A	T	4: 33,174,772 (GRCm39)	N371K	probably damaging	Het
Ppp2r5d	A	G	17: 46,995,610 (GRCm39)	Y480H	probably benign	Het
Prkd1	A	T	12: 50,472,298 (GRCm39)	V205E	probably damaging	Het
R3hdm2	A	G	10: 127,312,559 (GRCm39)	I434V	probably benign	Het
Rel	T	A	11: 23,695,546 (GRCm39)	T246S	probably damaging	Het
Rnf215	T	A	11: 4,086,719 (GRCm39)	V172E	probably damaging	Het
Scara3	T	C	14: 66,158,553 (GRCm39)	D485G	probably damaging	Het
Sec16a	A	T	2: 26,321,169 (GRCm39)	Y1308N	probably damaging	Het
Sis	A	T	3: 72,839,393 (GRCm39)	D824E	possibly damaging	Het
Slc27a4	T	A	2: 29,701,202 (GRCm39)	V331E	probably damaging	Het
Srebf1	C	T	11: 60,091,528 (GRCm39)	R999H	probably benign	Het
St3gal3	A	C	4: 117,964,859 (GRCm39)	M1R	probably null	Het
Tapbp	C	T	17: 34,138,914 (GRCm39)		probably null	Het
Tep1	A	T	14: 51,103,494 (GRCm39)	N265K	probably damaging	Het
Thoc2l	T	A	5: 104,666,123 (GRCm39)	I215N	probably damaging	Het
Trim43a	G	T	9: 88,470,211 (GRCm39)	W339L	probably damaging	Het
Ttc22	T	C	4: 106,479,977 (GRCm39)	F77S	probably damaging	Het
Ttc8	A	G	12: 98,887,023 (GRCm39)	E32G	probably benign	Het
Uhmk1	A	T	1: 170,036,222 (GRCm39)		probably null	Het
Usp17lc	A	G	7: 103,068,148 (GRCm39)	H481R	possibly damaging	Het
Vamp8	G	A	6: 72,362,617 (GRCm39)	T35M	probably damaging	Het
Vill	A	G	9: 118,894,654 (GRCm39)	Y53C	probably damaging	Het
Vmn1r222	A	T	13: 23,416,932 (GRCm39)	S94T	probably damaging	Het
Vmn2r93	A	G	17: 18,525,413 (GRCm39)	Y357C	possibly damaging	Het
Vps13c	T	A	9: 67,800,394 (GRCm39)	V536D	possibly damaging	Het
Vrtn	G	A	12: 84,696,855 (GRCm39)	C535Y	probably damaging	Het
Vwa3a	A	G	7: 120,367,388 (GRCm39)	Y181C	probably damaging	Het
Wfikkn2	G	A	11: 94,129,721 (GRCm39)	T140I	probably damaging	Het
Ykt6	T	A	11: 5,912,349 (GRCm39)	F101I	probably damaging	Het
Zfp277	C	T	12: 40,428,825 (GRCm39)	G174D	probably benign	Het
Zfp638	A	G	6: 83,955,047 (GRCm39)		probably null	Het
Zzef1	C	T	11: 72,815,505 (GRCm39)	P2942S	probably damaging	Het

Other mutations in Tln2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00468:Tln2	APN	9	67,251,469 (GRCm39)	missense	possibly damaging	0.59
IGL01110:Tln2	APN	9	67,157,864 (GRCm39)	nonsense	probably null
IGL01112:Tln2	APN	9	67,219,093 (GRCm39)	missense	probably damaging	1.00
IGL01307:Tln2	APN	9	67,302,749 (GRCm39)	missense	probably benign	0.25
IGL01374:Tln2	APN	9	67,169,205 (GRCm39)	missense	probably damaging	1.00
IGL01625:Tln2	APN	9	67,277,905 (GRCm39)	missense	probably damaging	1.00
IGL01865:Tln2	APN	9	67,157,896 (GRCm39)	nonsense	probably null
IGL01999:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02002:Tln2	APN	9	67,263,980 (GRCm39)	missense	probably damaging	0.98
IGL02005:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02015:Tln2	APN	9	67,268,721 (GRCm39)	splice site	probably benign
IGL02368:Tln2	APN	9	67,148,092 (GRCm39)	splice site	probably benign
IGL02444:Tln2	APN	9	67,165,874 (GRCm39)	splice site	probably benign
IGL02646:Tln2	APN	9	67,163,278 (GRCm39)	missense	probably benign	0.43
IGL02744:Tln2	APN	9	67,136,658 (GRCm39)	nonsense	probably null
IGL02869:Tln2	APN	9	67,128,807 (GRCm39)	splice site	probably benign
IGL02930:Tln2	APN	9	67,300,944 (GRCm39)	nonsense	probably null
IGL03100:Tln2	APN	9	67,203,019 (GRCm39)	missense	probably damaging	1.00
IGL03326:Tln2	APN	9	67,241,539 (GRCm39)	missense	possibly damaging	0.67
Harrier	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
Marsh	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
BB008:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
BB018:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0107:Tln2	UTSW	9	67,277,988 (GRCm39)	missense	probably damaging	1.00
R0494:Tln2	UTSW	9	67,262,479 (GRCm39)	missense	probably benign	0.22
R0884:Tln2	UTSW	9	67,278,015 (GRCm39)	missense	probably damaging	1.00
R0947:Tln2	UTSW	9	67,203,095 (GRCm39)	missense	probably benign	0.08
R0989:Tln2	UTSW	9	67,136,736 (GRCm39)	missense	probably damaging	1.00
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1486:Tln2	UTSW	9	67,219,121 (GRCm39)	missense	probably damaging	1.00
R1527:Tln2	UTSW	9	67,179,950 (GRCm39)	missense	possibly damaging	0.95
R1636:Tln2	UTSW	9	67,213,814 (GRCm39)	missense	probably damaging	1.00
R1656:Tln2	UTSW	9	67,134,389 (GRCm39)	missense	possibly damaging	0.81
R1707:Tln2	UTSW	9	67,283,089 (GRCm39)	missense	probably benign	0.00
R1749:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1751:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1761:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1767:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1815:Tln2	UTSW	9	67,136,705 (GRCm39)	missense	probably damaging	1.00
R1840:Tln2	UTSW	9	67,249,325 (GRCm39)	missense	probably damaging	1.00
R1847:Tln2	UTSW	9	67,269,969 (GRCm39)	nonsense	probably null
R1964:Tln2	UTSW	9	67,249,417 (GRCm39)	missense	probably benign	0.00
R1968:Tln2	UTSW	9	67,163,183 (GRCm39)	missense	probably damaging	1.00
R2036:Tln2	UTSW	9	67,179,986 (GRCm39)	missense	possibly damaging	0.76
R2038:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R2152:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2153:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2154:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2191:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2192:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2201:Tln2	UTSW	9	67,283,039 (GRCm39)	missense	probably damaging	1.00
R3116:Tln2	UTSW	9	67,262,421 (GRCm39)	missense	probably benign	0.10
R3151:Tln2	UTSW	9	67,237,829 (GRCm39)	critical splice donor site	probably null
R3795:Tln2	UTSW	9	67,163,197 (GRCm39)	missense	probably damaging	0.97
R3953:Tln2	UTSW	9	67,277,911 (GRCm39)	missense	probably damaging	1.00
R4450:Tln2	UTSW	9	67,251,347 (GRCm39)	critical splice donor site	probably null
R4685:Tln2	UTSW	9	67,209,854 (GRCm39)	missense	probably damaging	1.00
R4688:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R4696:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4697:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4700:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4701:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4741:Tln2	UTSW	9	67,293,837 (GRCm39)	critical splice donor site	probably null
R4806:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4807:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4808:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4967:Tln2	UTSW	9	67,262,407 (GRCm39)	missense	probably damaging	0.97
R5061:Tln2	UTSW	9	67,261,750 (GRCm39)	missense	probably benign
R5092:Tln2	UTSW	9	67,163,310 (GRCm39)	missense	probably benign	0.13
R5093:Tln2	UTSW	9	67,241,596 (GRCm39)	missense	probably benign	0.44
R5126:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R5204:Tln2	UTSW	9	67,261,764 (GRCm39)	missense	probably benign	0.00
R5236:Tln2	UTSW	9	67,273,205 (GRCm39)	missense	probably damaging	0.99
R5287:Tln2	UTSW	9	67,149,641 (GRCm39)	missense	probably damaging	1.00
R5568:Tln2	UTSW	9	67,219,147 (GRCm39)	missense	probably damaging	1.00
R5571:Tln2	UTSW	9	67,241,602 (GRCm39)	missense	possibly damaging	0.88
R5642:Tln2	UTSW	9	67,203,640 (GRCm39)	missense	probably benign	0.01
R5711:Tln2	UTSW	9	67,299,829 (GRCm39)	missense	probably benign	0.00
R5776:Tln2	UTSW	9	67,165,532 (GRCm39)	missense	probably damaging	1.00
R5791:Tln2	UTSW	9	67,293,887 (GRCm39)	missense	probably damaging	0.98
R5866:Tln2	UTSW	9	67,174,150 (GRCm39)	missense	probably damaging	1.00
R5888:Tln2	UTSW	9	67,136,685 (GRCm39)	missense	probably damaging	1.00
R5902:Tln2	UTSW	9	67,269,999 (GRCm39)	missense	probably benign	0.02
R6106:Tln2	UTSW	9	67,230,302 (GRCm39)	missense	probably damaging	0.99
R6175:Tln2	UTSW	9	67,131,363 (GRCm39)	missense	probably damaging	1.00
R6385:Tln2	UTSW	9	67,185,411 (GRCm39)	missense	probably benign	0.45
R6430:Tln2	UTSW	9	67,179,947 (GRCm39)	missense	probably damaging	1.00
R6441:Tln2	UTSW	9	67,179,971 (GRCm39)	missense	probably damaging	1.00
R6738:Tln2	UTSW	9	67,293,946 (GRCm39)	missense	possibly damaging	0.91
R6776:Tln2	UTSW	9	67,170,187 (GRCm39)	missense	probably damaging	1.00
R6794:Tln2	UTSW	9	67,193,840 (GRCm39)	missense	probably benign	0.07
R6850:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R6907:Tln2	UTSW	9	67,304,917 (GRCm39)	missense	probably damaging	0.98
R6909:Tln2	UTSW	9	67,299,814 (GRCm39)	missense	probably damaging	0.97
R6951:Tln2	UTSW	9	67,165,767 (GRCm39)	missense	probably damaging	0.97
R7015:Tln2	UTSW	9	67,269,929 (GRCm39)	missense	possibly damaging	0.55
R7051:Tln2	UTSW	9	67,253,699 (GRCm39)	missense	probably benign	0.00
R7246:Tln2	UTSW	9	67,170,261 (GRCm39)	missense	probably damaging	1.00
R7292:Tln2	UTSW	9	67,253,743 (GRCm39)	missense	probably benign
R7753:Tln2	UTSW	9	67,302,755 (GRCm39)	missense	probably damaging	1.00
R7868:Tln2	UTSW	9	67,255,508 (GRCm39)	missense	probably damaging	1.00
R7931:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R8023:Tln2	UTSW	9	67,131,346 (GRCm39)	missense	probably damaging	1.00
R8081:Tln2	UTSW	9	67,264,029 (GRCm39)	missense	probably damaging	1.00
R8164:Tln2	UTSW	9	67,226,702 (GRCm39)	missense	probably benign	0.31
R8192:Tln2	UTSW	9	67,253,811 (GRCm39)	nonsense	probably null
R8495:Tln2	UTSW	9	67,261,749 (GRCm39)	missense	probably benign	0.01
R8734:Tln2	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
R8739:Tln2	UTSW	9	67,165,555 (GRCm39)	missense	probably damaging	1.00
R8757:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8759:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8770:Tln2	UTSW	9	67,230,304 (GRCm39)	missense	probably benign
R8781:Tln2	UTSW	9	67,163,233 (GRCm39)	missense	probably damaging	1.00
R8812:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8814:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8816:Tln2	UTSW	9	67,128,799 (GRCm39)	missense	probably damaging	1.00
R8816:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8833:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8835:Tln2	UTSW	9	67,304,975 (GRCm39)	splice site	probably benign
R8837:Tln2	UTSW	9	67,157,866 (GRCm39)	missense	probably damaging	0.99
R8843:Tln2	UTSW	9	67,302,827 (GRCm39)	missense	probably damaging	1.00
R8864:Tln2	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
R8867:Tln2	UTSW	9	67,237,832 (GRCm39)	missense	probably damaging	0.98
R8921:Tln2	UTSW	9	67,174,105 (GRCm39)	missense	probably damaging	0.99
R9080:Tln2	UTSW	9	67,253,843 (GRCm39)	missense	probably damaging	1.00
R9083:Tln2	UTSW	9	67,269,927 (GRCm39)	missense	probably damaging	0.96
R9150:Tln2	UTSW	9	67,128,778 (GRCm39)	missense	probably damaging	1.00
R9287:Tln2	UTSW	9	67,277,980 (GRCm39)	missense	probably benign	0.20
R9330:Tln2	UTSW	9	67,229,213 (GRCm39)	missense	possibly damaging	0.61
R9343:Tln2	UTSW	9	67,230,353 (GRCm39)	missense	probably benign	0.10
R9355:Tln2	UTSW	9	67,262,529 (GRCm39)	missense	possibly damaging	0.46
R9383:Tln2	UTSW	9	67,278,043 (GRCm39)	missense	probably benign	0.17
R9386:Tln2	UTSW	9	67,273,249 (GRCm39)	missense	possibly damaging	0.78
R9407:Tln2	UTSW	9	67,136,732 (GRCm39)	missense	probably damaging	1.00
R9483:Tln2	UTSW	9	67,299,769 (GRCm39)	missense	probably damaging	1.00
R9523:Tln2	UTSW	9	67,165,766 (GRCm39)	missense	probably damaging	0.99
R9642:Tln2	UTSW	9	67,157,826 (GRCm39)	missense	probably benign	0.02
R9703:Tln2	UTSW	9	67,293,938 (GRCm39)	missense	probably damaging	1.00
X0027:Tln2	UTSW	9	67,284,135 (GRCm39)	missense	probably damaging	1.00
X0064:Tln2	UTSW	9	67,255,420 (GRCm39)	missense	probably damaging	1.00
X0067:Tln2	UTSW	9	67,277,973 (GRCm39)	missense	probably damaging	1.00
Z1176:Tln2	UTSW	9	67,253,767 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- CCAGTCCCAGGCACAAAGATTATTCTC -3'
(R):5'- TCATTTGGACGGAAATGGACTTACTCC -3'

Sequencing Primer
(F):5'- GTACCATCCACAGTAACAGAGTTATG -3'
(R):5'- TCATGACAGCATGGCTGG -3'

Posted On

2014-04-24