Mutagenetix > Incidental Mutation

Incidental Mutation 'R1589:Spen'

177709

Institutional Source

Beutler Lab

Gene Symbol

Spen

Ensembl Gene

ENSMUSG00000040761

Gene Name

spen family transcription repressor

Synonyms

Mint

MMRRC Submission

039626-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1589 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

141467890-141538597 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 141488024 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 499 (D499G)

Ref Sequence

ENSEMBL: ENSMUSP00000101412 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078886] [ENSMUST00000105786]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000078886
AA Change: D499G

SMART Domains

Protein: ENSMUSP00000077925
Gene: ENSMUSG00000040761
AA Change: D499G

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	617	632	N/A	INTRINSIC
low complexity region	669	691	N/A	INTRINSIC
low complexity region	695	720	N/A	INTRINSIC
low complexity region	749	773	N/A	INTRINSIC
coiled coil region	800	825	N/A	INTRINSIC
low complexity region	830	841	N/A	INTRINSIC
internal_repeat_2	844	954	6.27e-5	PROSPERO
coiled coil region	1494	1522	N/A	INTRINSIC
low complexity region	1587	1627	N/A	INTRINSIC
low complexity region	1635	1641	N/A	INTRINSIC
low complexity region	1642	1671	N/A	INTRINSIC
low complexity region	1747	1758	N/A	INTRINSIC
low complexity region	1810	1823	N/A	INTRINSIC
low complexity region	1888	1903	N/A	INTRINSIC
low complexity region	1940	1955	N/A	INTRINSIC
low complexity region	2003	2012	N/A	INTRINSIC
internal_repeat_2	2015	2115	6.27e-5	PROSPERO
low complexity region	2127	2147	N/A	INTRINSIC
low complexity region	2169	2191	N/A	INTRINSIC
low complexity region	2207	2219	N/A	INTRINSIC
low complexity region	2304	2323	N/A	INTRINSIC
low complexity region	2332	2371	N/A	INTRINSIC
low complexity region	2396	2413	N/A	INTRINSIC
low complexity region	2518	2533	N/A	INTRINSIC
low complexity region	2545	2555	N/A	INTRINSIC
low complexity region	2696	2722	N/A	INTRINSIC
low complexity region	2931	2942	N/A	INTRINSIC
low complexity region	2994	3006	N/A	INTRINSIC
low complexity region	3192	3212	N/A	INTRINSIC
low complexity region	3299	3337	N/A	INTRINSIC
Pfam:SPOC	3465	3586	2.7e-29	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000105786
AA Change: D499G

SMART Domains

Protein: ENSMUSP00000101412
Gene: ENSMUSG00000040761
AA Change: D499G

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	692	714	N/A	INTRINSIC
low complexity region	718	743	N/A	INTRINSIC
low complexity region	772	796	N/A	INTRINSIC
coiled coil region	823	848	N/A	INTRINSIC
low complexity region	853	864	N/A	INTRINSIC
internal_repeat_2	867	977	8.58e-5	PROSPERO
coiled coil region	1517	1545	N/A	INTRINSIC
low complexity region	1610	1650	N/A	INTRINSIC
low complexity region	1658	1664	N/A	INTRINSIC
low complexity region	1665	1694	N/A	INTRINSIC
low complexity region	1770	1781	N/A	INTRINSIC
low complexity region	1833	1846	N/A	INTRINSIC
low complexity region	1911	1926	N/A	INTRINSIC
low complexity region	1963	1978	N/A	INTRINSIC
low complexity region	2026	2035	N/A	INTRINSIC
internal_repeat_2	2038	2138	8.58e-5	PROSPERO
low complexity region	2150	2170	N/A	INTRINSIC
low complexity region	2192	2214	N/A	INTRINSIC
low complexity region	2230	2242	N/A	INTRINSIC
low complexity region	2327	2346	N/A	INTRINSIC
low complexity region	2355	2394	N/A	INTRINSIC
low complexity region	2419	2436	N/A	INTRINSIC
low complexity region	2541	2556	N/A	INTRINSIC
low complexity region	2568	2578	N/A	INTRINSIC
low complexity region	2719	2745	N/A	INTRINSIC
low complexity region	2954	2965	N/A	INTRINSIC
low complexity region	3017	3029	N/A	INTRINSIC
low complexity region	3215	3235	N/A	INTRINSIC
low complexity region	3322	3360	N/A	INTRINSIC
Pfam:SPOC	3488	3609	2.7e-29	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152411

Meta Mutation Damage Score

0.6995

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 95.8%
20x: 90.8%

Validation Efficiency

97% (89/92)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice die during late gestation exhibiting morphological abnormalities of the heart, pancreas, and liver. Inactivation of this gene also affects the differentiation of B cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700008P02Rik	A	G	3: 6,620,441 (GRCm38)		probably benign	Het
Aadacl2	C	T	3: 60,010,576 (GRCm38)	T82I	probably benign	Het
Acadl	T	C	1: 66,853,223 (GRCm38)	N147S	probably benign	Het
Actr3	A	T	1: 125,408,563 (GRCm38)	M79K	probably damaging	Het
Agbl3	A	G	6: 34,857,517 (GRCm38)	S874G	possibly damaging	Het
Aoah	A	T	13: 21,002,948 (GRCm38)	T472S	probably damaging	Het
Asb16	A	T	11: 102,268,995 (GRCm38)	D58V	probably damaging	Het
B4galt1	T	C	4: 40,823,575 (GRCm38)	D172G	probably benign	Het
Bax	T	C	7: 45,465,247 (GRCm38)	N55S	possibly damaging	Het
Bdkrb2	A	G	12: 105,591,859 (GRCm38)	N120D	possibly damaging	Het
Bicdl1	T	C	5: 115,651,266 (GRCm38)		probably benign	Het
Cand1	A	G	10: 119,213,566 (GRCm38)	L425P	probably damaging	Het
Caskin1	G	A	17: 24,505,478 (GRCm38)		probably null	Het
Cd248	C	T	19: 5,069,932 (GRCm38)	P603S	probably benign	Het
Cep95	G	T	11: 106,800,104 (GRCm38)	R143L	probably benign	Het
Clptm1l	G	T	13: 73,614,673 (GRCm38)		probably null	Het
Cntnap5a	T	C	1: 116,060,200 (GRCm38)	F154L	possibly damaging	Het
Cyld	A	T	8: 88,709,990 (GRCm38)	I303F	possibly damaging	Het
Dock2	A	G	11: 34,706,461 (GRCm38)	S370P	probably damaging	Het
Enah	G	T	1: 181,922,293 (GRCm38)	T327K	probably damaging	Het
Farp2	A	T	1: 93,579,860 (GRCm38)	S427C	probably damaging	Het
Fbxw11	C	T	11: 32,733,612 (GRCm38)	T301M	probably damaging	Het
Foxc2	A	T	8: 121,117,176 (GRCm38)	T188S	probably benign	Het
Fzd2	A	G	11: 102,606,328 (GRCm38)	T533A	probably benign	Het
Glb1l2	G	T	9: 26,769,038 (GRCm38)	S248*	probably null	Het
Glul	A	T	1: 153,905,538 (GRCm38)		probably benign	Het
Gm28042	A	G	2: 120,041,406 (GRCm38)	T946A	probably benign	Het
Golga3	T	A	5: 110,181,783 (GRCm38)	D2E	probably damaging	Het
Grm1	A	T	10: 10,719,967 (GRCm38)	F639Y	probably benign	Het
Gzmn	A	G	14: 56,165,911 (GRCm38)	L247P	probably benign	Het
Hgf	T	G	5: 16,613,785 (GRCm38)	I525R	probably damaging	Het
Hnrnpul2	T	A	19: 8,831,332 (GRCm38)	D719E	probably benign	Het
Itga10	C	T	3: 96,651,738 (GRCm38)		probably benign	Het
Itga9	T	C	9: 118,607,117 (GRCm38)		probably null	Het
Itgb1	T	A	8: 128,705,458 (GRCm38)	C16S	probably damaging	Het
Itgb1	G	T	8: 128,705,459 (GRCm38)	C16F	possibly damaging	Het
Kat14	A	G	2: 144,394,100 (GRCm38)	I251V	probably benign	Het
Kdsr	A	T	1: 106,734,541 (GRCm38)		probably null	Het
Kif12	T	A	4: 63,166,500 (GRCm38)	E527V	probably benign	Het
Larp1b	T	C	3: 41,033,474 (GRCm38)	S44P	probably damaging	Het
Lonp2	T	C	8: 86,673,072 (GRCm38)		probably benign	Het
Lrp1	C	T	10: 127,605,606 (GRCm38)	S216N	probably benign	Het
Lrrc19	T	C	4: 94,640,950 (GRCm38)	S32G	probably benign	Het
Mdm2	G	A	10: 117,690,529 (GRCm38)	T335M	probably benign	Het
Mettl25	A	G	10: 105,779,632 (GRCm38)	Y504H	probably damaging	Het
Mill1	T	C	7: 18,245,647 (GRCm38)	I13T	probably benign	Het
Mmachc	T	A	4: 116,703,524 (GRCm38)	Q258L	probably benign	Het
Mpdz	T	A	4: 81,421,176 (GRCm38)	I5L	probably benign	Het
Mrps2	C	T	2: 28,469,488 (GRCm38)	A119V	probably benign	Het
Mtcl2	A	T	2: 157,027,637 (GRCm38)	M1026K	probably benign	Het
Mtmr11	A	G	3: 96,168,113 (GRCm38)	T370A	probably benign	Het
Nmi	T	C	2: 51,958,977 (GRCm38)	I34V	possibly damaging	Het
Obscn	A	T	11: 59,036,075 (GRCm38)	M5538K	possibly damaging	Het
Ogdhl	T	C	14: 32,325,865 (GRCm38)	I24T	probably benign	Het
Omp	G	T	7: 98,145,359 (GRCm38)	D20E	probably benign	Het
Or2ag19	A	G	7: 106,845,196 (GRCm38)	Y195C	possibly damaging	Het
Or52e8b	A	G	7: 105,024,560 (GRCm38)	V140A	probably benign	Het
Or5b120	C	T	19: 13,502,757 (GRCm38)	T138M	probably benign	Het
Or6c69	A	G	10: 129,911,681 (GRCm38)	V199A	probably benign	Het
Otog	A	G	7: 46,283,908 (GRCm38)	H1291R	probably benign	Het
Pgbd1	A	G	13: 21,423,292 (GRCm38)	L244P	probably damaging	Het
Ranbp2	A	G	10: 58,463,986 (GRCm38)	I481V	probably benign	Het
Rbp3	A	T	14: 33,955,792 (GRCm38)	I566F	probably damaging	Het
Rsph4a	A	G	10: 33,905,529 (GRCm38)	D125G	probably benign	Het
Scn11a	A	G	9: 119,769,807 (GRCm38)	V1219A	probably damaging	Het
Serpine3	G	A	14: 62,674,381 (GRCm38)	G264D	probably benign	Het
Slc4a10	T	C	2: 62,257,462 (GRCm38)	F400L	probably damaging	Het
Slco1a4	C	T	6: 141,845,447 (GRCm38)	V8I	probably benign	Het
Stk32c	A	G	7: 139,119,015 (GRCm38)		probably null	Het
Tars1	A	G	15: 11,388,175 (GRCm38)	V485A	probably benign	Het
Tcerg1l	A	G	7: 138,361,767 (GRCm38)	L258P	probably damaging	Het
Tmc2	A	T	2: 130,247,960 (GRCm38)	I622F	probably damaging	Het
Tmem183a	A	T	1: 134,354,706 (GRCm38)	N220K	probably damaging	Het
Tnni3	T	C	7: 4,520,526 (GRCm38)	D146G	probably damaging	Het
Trappc11	G	T	8: 47,501,680 (GRCm38)	D908E	probably damaging	Het
Trappc8	A	G	18: 20,863,551 (GRCm38)	Y436H	probably damaging	Het
Ttc41	G	A	10: 86,776,390 (GRCm38)	V1176I	probably benign	Het
Ube2b	A	G	11: 51,997,872 (GRCm38)	V24A	probably benign	Het
Usp30	T	C	5: 114,112,961 (GRCm38)	C233R	probably damaging	Het
Vmn1r87	T	A	7: 13,131,776 (GRCm38)	T195S	possibly damaging	Het
Vmn2r24	T	C	6: 123,806,520 (GRCm38)		probably benign	Het
Vmn2r81	C	A	10: 79,293,024 (GRCm38)	T583N	probably damaging	Het
Vwa8	C	T	14: 78,908,230 (GRCm38)	R116C	probably damaging	Het
Wdr1	C	A	5: 38,529,972 (GRCm38)	V239L	probably benign	Het
Wdr17	C	T	8: 54,703,907 (GRCm38)		probably benign	Het
Zfhx4	A	C	3: 5,241,729 (GRCm38)	D5A	probably damaging	Het
Zfyve27	T	C	19: 42,171,745 (GRCm38)		probably null	Het

Other mutations in Spen

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Spen	APN	4	141,489,901 (GRCm38)	missense	unknown
IGL01357:Spen	APN	4	141,517,113 (GRCm38)	missense	unknown
IGL02184:Spen	APN	4	141,487,606 (GRCm38)	missense	unknown
IGL02226:Spen	APN	4	141,478,146 (GRCm38)	missense	unknown
IGL02321:Spen	APN	4	141,517,130 (GRCm38)	missense	unknown
IGL02350:Spen	APN	4	141,477,579 (GRCm38)	missense	unknown
IGL02357:Spen	APN	4	141,477,579 (GRCm38)	missense	unknown
IGL02627:Spen	APN	4	141,473,015 (GRCm38)	missense	probably damaging	0.99
IGL02683:Spen	APN	4	141,471,645 (GRCm38)	missense	probably benign	0.06
IGL02945:Spen	APN	4	141,494,313 (GRCm38)	missense	unknown
IGL02950:Spen	APN	4	141,469,508 (GRCm38)	missense	probably damaging	1.00
IGL03008:Spen	APN	4	141,476,137 (GRCm38)	missense	possibly damaging	0.70
IGL03019:Spen	APN	4	141,478,916 (GRCm38)	missense	unknown
IGL03038:Spen	APN	4	141,538,239 (GRCm38)	missense	unknown
IGL03334:Spen	APN	4	141,469,969 (GRCm38)	missense	probably damaging	1.00
filtered	UTSW	4	141,477,372 (GRCm38)	missense	unknown
mentholated	UTSW	4	141,469,400 (GRCm38)	missense	possibly damaging	0.78
R0105:Spen	UTSW	4	141,469,810 (GRCm38)	splice site	probably benign
R0268:Spen	UTSW	4	141,477,557 (GRCm38)	missense	unknown
R0359:Spen	UTSW	4	141,516,870 (GRCm38)	missense	unknown
R0394:Spen	UTSW	4	141,474,203 (GRCm38)	missense	probably benign	0.03
R0423:Spen	UTSW	4	141,479,336 (GRCm38)	missense	unknown
R0433:Spen	UTSW	4	141,483,758 (GRCm38)	missense	unknown
R0462:Spen	UTSW	4	141,473,651 (GRCm38)	missense	probably damaging	1.00
R0687:Spen	UTSW	4	141,488,028 (GRCm38)	missense	unknown
R0699:Spen	UTSW	4	141,474,391 (GRCm38)	missense	possibly damaging	0.72
R0865:Spen	UTSW	4	141,471,870 (GRCm38)	missense	probably benign	0.11
R0918:Spen	UTSW	4	141,485,564 (GRCm38)	missense	unknown
R1034:Spen	UTSW	4	141,475,752 (GRCm38)	missense	probably benign	0.33
R1341:Spen	UTSW	4	141,469,400 (GRCm38)	missense	possibly damaging	0.78
R1401:Spen	UTSW	4	141,471,821 (GRCm38)	missense	probably damaging	0.98
R1509:Spen	UTSW	4	141,475,635 (GRCm38)	missense	probably benign	0.00
R1509:Spen	UTSW	4	141,475,700 (GRCm38)	missense	possibly damaging	0.53
R1561:Spen	UTSW	4	141,472,383 (GRCm38)	nonsense	probably null
R1640:Spen	UTSW	4	141,468,943 (GRCm38)	missense	probably damaging	0.98
R1758:Spen	UTSW	4	141,476,375 (GRCm38)	missense	unknown
R1764:Spen	UTSW	4	141,472,950 (GRCm38)	missense	probably damaging	1.00
R1824:Spen	UTSW	4	141,472,785 (GRCm38)	missense	probably damaging	1.00
R1899:Spen	UTSW	4	141,470,343 (GRCm38)	missense	probably benign	0.17
R1916:Spen	UTSW	4	141,472,598 (GRCm38)	missense	probably damaging	1.00
R2011:Spen	UTSW	4	141,473,329 (GRCm38)	missense	probably damaging	1.00
R2295:Spen	UTSW	4	141,477,273 (GRCm38)	missense	unknown
R2379:Spen	UTSW	4	141,516,927 (GRCm38)	missense	unknown
R2404:Spen	UTSW	4	141,477,905 (GRCm38)	missense	unknown
R3719:Spen	UTSW	4	141,517,183 (GRCm38)	missense	unknown
R3889:Spen	UTSW	4	141,477,881 (GRCm38)	missense	unknown
R3945:Spen	UTSW	4	141,477,353 (GRCm38)	missense	unknown
R4227:Spen	UTSW	4	141,522,147 (GRCm38)	missense	unknown
R4326:Spen	UTSW	4	141,477,372 (GRCm38)	missense	unknown
R4382:Spen	UTSW	4	141,473,139 (GRCm38)	missense	possibly damaging	0.88
R4542:Spen	UTSW	4	141,476,786 (GRCm38)	missense	unknown
R4757:Spen	UTSW	4	141,473,079 (GRCm38)	nonsense	probably null
R4771:Spen	UTSW	4	141,472,596 (GRCm38)	missense	probably benign	0.14
R5072:Spen	UTSW	4	141,522,302 (GRCm38)	missense	unknown
R5121:Spen	UTSW	4	141,476,099 (GRCm38)	missense	probably benign	0.00
R5176:Spen	UTSW	4	141,476,276 (GRCm38)	missense	unknown
R5290:Spen	UTSW	4	141,473,816 (GRCm38)	missense	probably damaging	1.00
R5291:Spen	UTSW	4	141,488,079 (GRCm38)	missense	unknown
R5293:Spen	UTSW	4	141,472,406 (GRCm38)	missense	possibly damaging	0.89
R5347:Spen	UTSW	4	141,471,485 (GRCm38)	missense	probably benign	0.26
R5511:Spen	UTSW	4	141,516,838 (GRCm38)	missense	unknown
R5511:Spen	UTSW	4	141,475,064 (GRCm38)	missense	possibly damaging	0.86
R5772:Spen	UTSW	4	141,478,184 (GRCm38)	missense	unknown
R5834:Spen	UTSW	4	141,471,843 (GRCm38)	missense	possibly damaging	0.63
R5858:Spen	UTSW	4	141,473,871 (GRCm38)	missense	probably benign	0.05
R6214:Spen	UTSW	4	141,479,112 (GRCm38)	missense	unknown
R6232:Spen	UTSW	4	141,517,022 (GRCm38)	missense	unknown
R6345:Spen	UTSW	4	141,471,633 (GRCm38)	missense	possibly damaging	0.86
R6419:Spen	UTSW	4	141,476,310 (GRCm38)	missense	unknown
R6455:Spen	UTSW	4	141,475,509 (GRCm38)	missense	probably damaging	0.97
R6979:Spen	UTSW	4	141,478,063 (GRCm38)	missense	unknown
R6994:Spen	UTSW	4	141,493,459 (GRCm38)	missense	unknown
R7018:Spen	UTSW	4	141,493,444 (GRCm38)	missense	unknown
R7040:Spen	UTSW	4	141,494,382 (GRCm38)	missense	unknown
R7127:Spen	UTSW	4	141,476,108 (GRCm38)	missense	possibly damaging	0.53
R7218:Spen	UTSW	4	141,472,650 (GRCm38)	missense	possibly damaging	0.54
R7234:Spen	UTSW	4	141,479,135 (GRCm38)	missense	unknown
R7316:Spen	UTSW	4	141,477,054 (GRCm38)	missense	unknown
R7350:Spen	UTSW	4	141,479,385 (GRCm38)	missense	unknown
R7356:Spen	UTSW	4	141,471,924 (GRCm38)	nonsense	probably null
R7400:Spen	UTSW	4	141,473,741 (GRCm38)	missense	probably damaging	1.00
R7470:Spen	UTSW	4	141,479,294 (GRCm38)	missense	unknown
R7698:Spen	UTSW	4	141,472,845 (GRCm38)	missense	probably damaging	1.00
R7858:Spen	UTSW	4	141,488,131 (GRCm38)	splice site	probably null
R8033:Spen	UTSW	4	141,471,746 (GRCm38)	missense	probably benign	0.03
R8064:Spen	UTSW	4	141,475,700 (GRCm38)	missense	possibly damaging	0.53
R8159:Spen	UTSW	4	141,475,003 (GRCm38)	missense	possibly damaging	0.53
R8187:Spen	UTSW	4	141,472,905 (GRCm38)	missense	possibly damaging	0.93
R8463:Spen	UTSW	4	141,522,279 (GRCm38)	missense	unknown
R8557:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8558:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8672:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8673:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8674:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8714:Spen	UTSW	4	141,488,003 (GRCm38)	missense	unknown
R8735:Spen	UTSW	4	141,469,818 (GRCm38)	missense	probably benign	0.32
R8762:Spen	UTSW	4	141,472,950 (GRCm38)	missense	probably damaging	1.00
R8877:Spen	UTSW	4	141,471,826 (GRCm38)	nonsense	probably null
R8878:Spen	UTSW	4	141,477,209 (GRCm38)	missense	unknown
R8937:Spen	UTSW	4	141,474,063 (GRCm38)	missense	probably damaging	1.00
R8939:Spen	UTSW	4	141,475,658 (GRCm38)	missense	possibly damaging	0.72
R8968:Spen	UTSW	4	141,470,390 (GRCm38)	missense	probably benign	0.02
R8971:Spen	UTSW	4	141,474,578 (GRCm38)	missense	possibly damaging	0.53
R9016:Spen	UTSW	4	141,473,627 (GRCm38)	missense	probably damaging	1.00
R9072:Spen	UTSW	4	141,476,391 (GRCm38)	missense	unknown
R9073:Spen	UTSW	4	141,476,391 (GRCm38)	missense	unknown
R9120:Spen	UTSW	4	141,472,922 (GRCm38)	missense
R9136:Spen	UTSW	4	141,522,312 (GRCm38)	missense	unknown
R9138:Spen	UTSW	4	141,469,486 (GRCm38)	missense	probably damaging	1.00
R9150:Spen	UTSW	4	141,517,157 (GRCm38)	missense	unknown
R9225:Spen	UTSW	4	141,475,632 (GRCm38)	missense	possibly damaging	0.53
R9492:Spen	UTSW	4	141,471,787 (GRCm38)	missense	probably benign	0.26
R9537:Spen	UTSW	4	141,516,845 (GRCm38)	small deletion	probably benign
R9537:Spen	UTSW	4	141,471,704 (GRCm38)	missense	probably benign	0.15
R9602:Spen	UTSW	4	141,477,872 (GRCm38)	missense	unknown
R9609:Spen	UTSW	4	141,488,108 (GRCm38)	missense	unknown
R9686:Spen	UTSW	4	141,472,635 (GRCm38)	missense	probably benign	0.27
R9697:Spen	UTSW	4	141,468,964 (GRCm38)	missense	probably damaging	1.00
R9713:Spen	UTSW	4	141,517,020 (GRCm38)	missense	unknown
T0722:Spen	UTSW	4	141,474,353 (GRCm38)	missense	probably benign	0.33
T0975:Spen	UTSW	4	141,474,353 (GRCm38)	missense	probably benign	0.33
Z1088:Spen	UTSW	4	141,477,976 (GRCm38)	missense	unknown
Z1088:Spen	UTSW	4	141,477,977 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TAGCATGTGTCACCTGCCAGTGAG -3'
(R):5'- ACATAGTTGCTTTTCTCCAGGAGCC -3'

Sequencing Primer
(F):5'- GCCACACCCTCAAGTGTTC -3'
(R):5'- TGCGTACATGATCCTCTAAGCAG -3'

Posted On

2014-04-24