Incidental Mutation 'R1589:Vmn2r81'
ID177742
Institutional Source Beutler Lab
Gene Symbol Vmn2r81
Ensembl Gene ENSMUSG00000055515
Gene Namevomeronasal 2, receptor 81
SynonymsV2rf2, EC1-VR2, pheromone recepter
MMRRC Submission 039626-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.067) question?
Stock #R1589 (G1)
Quality Score225
Status Validated
Chromosome10
Chromosomal Location79247777-79294535 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to A at 79293024 bp
ZygosityHeterozygous
Amino Acid Change Threonine to Asparagine at position 583 (T583N)
Ref Sequence ENSEMBL: ENSMUSP00000020547 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020547]
Predicted Effect probably damaging
Transcript: ENSMUST00000020547
AA Change: T583N

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000020547
Gene: ENSMUSG00000055515
AA Change: T583N

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
Pfam:ANF_receptor 79 474 7.7e-37 PFAM
Pfam:NCD3G 517 570 8.9e-21 PFAM
Pfam:7tm_3 603 838 6.1e-50 PFAM
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.2%
  • 10x: 95.8%
  • 20x: 90.8%
Validation Efficiency 97% (89/92)
MGI Phenotype PHENOTYPE: Mice homozygous for a knock-out allele exhibit vomeronasal sensory neuron axons that spread out diffusely over the posterior accessory olfactory bulb and extend abnormally into the anterior olfactory bulb. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 87 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700008P02Rik A G 3: 6,620,441 probably benign Het
Aadacl2 C T 3: 60,010,576 T82I probably benign Het
Acadl T C 1: 66,853,223 N147S probably benign Het
Actr3 A T 1: 125,408,563 M79K probably damaging Het
Agbl3 A G 6: 34,857,517 S874G possibly damaging Het
Aoah A T 13: 21,002,948 T472S probably damaging Het
Asb16 A T 11: 102,268,995 D58V probably damaging Het
B4galt1 T C 4: 40,823,575 D172G probably benign Het
Bax T C 7: 45,465,247 N55S possibly damaging Het
Bdkrb2 A G 12: 105,591,859 N120D possibly damaging Het
Bicdl1 T C 5: 115,651,266 probably benign Het
Cand1 A G 10: 119,213,566 L425P probably damaging Het
Caskin1 G A 17: 24,505,478 probably null Het
Cd248 C T 19: 5,069,932 P603S probably benign Het
Cep95 G T 11: 106,800,104 R143L probably benign Het
Clptm1l G T 13: 73,614,673 probably null Het
Cntnap5a T C 1: 116,060,200 F154L possibly damaging Het
Cyld A T 8: 88,709,990 I303F possibly damaging Het
Dock2 A G 11: 34,706,461 S370P probably damaging Het
Enah G T 1: 181,922,293 T327K probably damaging Het
Farp2 A T 1: 93,579,860 S427C probably damaging Het
Fbxw11 C T 11: 32,733,612 T301M probably damaging Het
Foxc2 A T 8: 121,117,176 T188S probably benign Het
Fzd2 A G 11: 102,606,328 T533A probably benign Het
Glb1l2 G T 9: 26,769,038 S248* probably null Het
Glul A T 1: 153,905,538 probably benign Het
Gm28042 A G 2: 120,041,406 T946A probably benign Het
Golga3 T A 5: 110,181,783 D2E probably damaging Het
Grm1 A T 10: 10,719,967 F639Y probably benign Het
Gzmn A G 14: 56,165,911 L247P probably benign Het
Hgf T G 5: 16,613,785 I525R probably damaging Het
Hnrnpul2 T A 19: 8,831,332 D719E probably benign Het
Itga10 C T 3: 96,651,738 probably benign Het
Itga9 T C 9: 118,607,117 probably null Het
Itgb1 T A 8: 128,705,458 C16S probably damaging Het
Itgb1 G T 8: 128,705,459 C16F possibly damaging Het
Kat14 A G 2: 144,394,100 I251V probably benign Het
Kdsr A T 1: 106,734,541 probably null Het
Kif12 T A 4: 63,166,500 E527V probably benign Het
Larp1b T C 3: 41,033,474 S44P probably damaging Het
Lonp2 T C 8: 86,673,072 probably benign Het
Lrp1 C T 10: 127,605,606 S216N probably benign Het
Lrrc19 T C 4: 94,640,950 S32G probably benign Het
Mdm2 G A 10: 117,690,529 T335M probably benign Het
Mettl25 A G 10: 105,779,632 Y504H probably damaging Het
Mill1 T C 7: 18,245,647 I13T probably benign Het
Mmachc T A 4: 116,703,524 Q258L probably benign Het
Mpdz T A 4: 81,421,176 I5L probably benign Het
Mrps2 C T 2: 28,469,488 A119V probably benign Het
Mtmr11 A G 3: 96,168,113 T370A probably benign Het
Nmi T C 2: 51,958,977 I34V possibly damaging Het
Obscn A T 11: 59,036,075 M5538K possibly damaging Het
Ogdhl T C 14: 32,325,865 I24T probably benign Het
Olfr1477 C T 19: 13,502,757 T138M probably benign Het
Olfr675 A G 7: 105,024,560 V140A probably benign Het
Olfr703 A G 7: 106,845,196 Y195C possibly damaging Het
Olfr816 A G 10: 129,911,681 V199A probably benign Het
Omp G T 7: 98,145,359 D20E probably benign Het
Otog A G 7: 46,283,908 H1291R probably benign Het
Pgbd1 A G 13: 21,423,292 L244P probably damaging Het
Ranbp2 A G 10: 58,463,986 I481V probably benign Het
Rbp3 A T 14: 33,955,792 I566F probably damaging Het
Rsph4a A G 10: 33,905,529 D125G probably benign Het
Scn11a A G 9: 119,769,807 V1219A probably damaging Het
Serpine3 G A 14: 62,674,381 G264D probably benign Het
Slc4a10 T C 2: 62,257,462 F400L probably damaging Het
Slco1a4 C T 6: 141,845,447 V8I probably benign Het
Soga1 A T 2: 157,027,637 M1026K probably benign Het
Spen T C 4: 141,488,024 D499G unknown Het
Stk32c A G 7: 139,119,015 probably null Het
Tars A G 15: 11,388,175 V485A probably benign Het
Tcerg1l A G 7: 138,361,767 L258P probably damaging Het
Tmc2 A T 2: 130,247,960 I622F probably damaging Het
Tmem183a A T 1: 134,354,706 N220K probably damaging Het
Tnni3 T C 7: 4,520,526 D146G probably damaging Het
Trappc11 G T 8: 47,501,680 D908E probably damaging Het
Trappc8 A G 18: 20,863,551 Y436H probably damaging Het
Ttc41 G A 10: 86,776,390 V1176I probably benign Het
Ube2b A G 11: 51,997,872 V24A probably benign Het
Usp30 T C 5: 114,112,961 C233R probably damaging Het
Vmn1r87 T A 7: 13,131,776 T195S possibly damaging Het
Vmn2r24 T C 6: 123,806,520 probably benign Het
Vwa8 C T 14: 78,908,230 R116C probably damaging Het
Wdr1 C A 5: 38,529,972 V239L probably benign Het
Wdr17 C T 8: 54,703,907 probably benign Het
Zfhx4 A C 3: 5,241,729 D5A probably damaging Het
Zfyve27 T C 19: 42,171,745 probably null Het
Other mutations in Vmn2r81
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01111:Vmn2r81 APN 10 79247997 missense probably benign 0.20
IGL02255:Vmn2r81 APN 10 79247972 nonsense probably null
IGL02901:Vmn2r81 APN 10 79270730 missense probably damaging 0.98
R0383:Vmn2r81 UTSW 10 79293447 missense possibly damaging 0.95
R0555:Vmn2r81 UTSW 10 79293449 missense probably damaging 1.00
R0715:Vmn2r81 UTSW 10 79268600 missense probably damaging 1.00
R0967:Vmn2r81 UTSW 10 79248023 splice site probably benign
R1438:Vmn2r81 UTSW 10 79293857 missense probably benign 0.00
R1468:Vmn2r81 UTSW 10 79293662 missense probably damaging 1.00
R1468:Vmn2r81 UTSW 10 79293662 missense probably damaging 1.00
R1671:Vmn2r81 UTSW 10 79267431 missense probably benign 0.04
R1672:Vmn2r81 UTSW 10 79268278 missense probably damaging 0.99
R1728:Vmn2r81 UTSW 10 79270655 missense probably benign 0.08
R1784:Vmn2r81 UTSW 10 79270655 missense probably benign 0.08
R1931:Vmn2r81 UTSW 10 79293494 missense probably damaging 0.98
R1934:Vmn2r81 UTSW 10 79247794 start codon destroyed probably null 0.71
R1944:Vmn2r81 UTSW 10 79293737 missense probably damaging 1.00
R2102:Vmn2r81 UTSW 10 79293500 missense probably damaging 0.97
R2190:Vmn2r81 UTSW 10 79268251 missense possibly damaging 0.67
R2392:Vmn2r81 UTSW 10 79274682 missense probably damaging 1.00
R3770:Vmn2r81 UTSW 10 79270600 missense probably damaging 1.00
R4551:Vmn2r81 UTSW 10 79268407 missense possibly damaging 0.95
R4629:Vmn2r81 UTSW 10 79267442 missense probably damaging 0.99
R4691:Vmn2r81 UTSW 10 79293377 nonsense probably null
R4829:Vmn2r81 UTSW 10 79247801 missense possibly damaging 0.48
R4914:Vmn2r81 UTSW 10 79270523 missense probably null 1.00
R4996:Vmn2r81 UTSW 10 79293413 missense probably benign 0.04
R5242:Vmn2r81 UTSW 10 79293475 nonsense probably null
R5253:Vmn2r81 UTSW 10 79247986 missense probably benign 0.00
R5925:Vmn2r81 UTSW 10 79247803 missense probably damaging 1.00
R6169:Vmn2r81 UTSW 10 79268548 missense probably benign
R6270:Vmn2r81 UTSW 10 79293815 missense probably benign
R6525:Vmn2r81 UTSW 10 79293726 missense probably benign 0.11
R6813:Vmn2r81 UTSW 10 79268605 missense probably benign 0.00
R6886:Vmn2r81 UTSW 10 79268154 missense possibly damaging 0.90
R7200:Vmn2r81 UTSW 10 79270736 critical splice donor site probably null
R7350:Vmn2r81 UTSW 10 79268385 missense probably benign 0.03
R7504:Vmn2r81 UTSW 10 79268332 missense probably benign
R7832:Vmn2r81 UTSW 10 79293830 missense probably damaging 1.00
R7915:Vmn2r81 UTSW 10 79293830 missense probably damaging 1.00
X0067:Vmn2r81 UTSW 10 79293043 missense possibly damaging 0.46
Predicted Primers PCR Primer
(F):5'- TGGAACATGGTTGTAATAACAATGCAATGAG -3'
(R):5'- CCAGGTAAGCCAGAGTGCAGTAGAA -3'

Sequencing Primer
(F):5'- ACAATGCAATGAGGTTGAACTC -3'
(R):5'- GAGGAGATGTTCCCAGCCATATAC -3'
Posted On2014-04-24