Incidental Mutation 'IGL01995:Cry2'
ID 183652
Institutional Source Australian Phenomics Network (link to record)
Gene Symbol Cry2
Ensembl Gene ENSMUSG00000068742
Gene Name cryptochrome 2 (photolyase-like)
Synonyms
Accession Numbers
Is this an essential gene? Possibly essential (E-score: 0.604) question?
Stock # IGL01995
Quality Score
Status
Chromosome 2
Chromosomal Location 92403646-92434043 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to C at 92424632 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Glycine at position 126 (E126G)
Ref Sequence ENSEMBL: ENSMUSP00000106909 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]
AlphaFold Q9R194
Predicted Effect probably benign
Transcript: ENSMUST00000090559
AA Change: E126G

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)
SMART Domains Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: E126G

DomainStartEndE-ValueType
low complexity region 2 13 N/A INTRINSIC
Pfam:DNA_photolyase 23 187 2.4e-50 PFAM
Pfam:FAD_binding_7 231 504 4.4e-89 PFAM
low complexity region 562 573 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000111278
AA Change: E126G

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)
SMART Domains Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: E126G

DomainStartEndE-ValueType
low complexity region 2 13 N/A INTRINSIC
Pfam:DNA_photolyase 23 189 3.6e-50 PFAM
Pfam:FAD_binding_7 230 506 1.4e-105 PFAM
low complexity region 562 573 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000125488
Predicted Effect noncoding transcript
Transcript: ENSMUST00000126002
Predicted Effect noncoding transcript
Transcript: ENSMUST00000144842
Coding Region Coverage
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 43 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930503E14Rik T C 14: 44,163,845 probably benign Het
AI661453 C A 17: 47,468,517 probably benign Het
Arhgap29 G A 3: 122,014,328 V1052I probably benign Het
Arhgef17 A G 7: 100,928,655 S1029P probably benign Het
Arpc5l T C 2: 39,008,214 S7P probably damaging Het
Asf1b A G 8: 83,955,904 I26V probably benign Het
Cep95 T C 11: 106,806,371 L240P probably damaging Het
Cnmd A G 14: 79,642,068 probably benign Het
Cntn3 T A 6: 102,203,885 N783I probably damaging Het
Col6a2 C A 10: 76,604,842 probably benign Het
Col9a2 T C 4: 121,050,410 probably null Het
Cxcl15 T C 5: 90,794,608 V13A possibly damaging Het
Dbr1 A G 9: 99,583,899 D510G probably benign Het
Dct A G 14: 118,034,243 F392L probably damaging Het
Dgkz A G 2: 91,934,050 probably benign Het
Dhrs7b A G 11: 60,830,715 I2V probably benign Het
Efnb3 A T 11: 69,556,904 probably null Het
Fbf1 T A 11: 116,151,020 R565S probably null Het
Gimap5 T C 6: 48,752,793 I99T probably damaging Het
Gm9912 A G 3: 149,185,449 L16P unknown Het
Gpr17 A G 18: 31,947,134 L292P probably damaging Het
Hdac10 C T 15: 89,127,598 V129M probably damaging Het
Mfap3l A G 8: 60,671,464 I247V possibly damaging Het
Mtus1 A T 8: 41,084,420 S86R probably damaging Het
Mylk3 A G 8: 85,359,261 S55P possibly damaging Het
Nol6 A T 4: 41,118,567 C745S probably damaging Het
Nudt13 A T 14: 20,306,317 E103D probably damaging Het
Olfr1129 A G 2: 87,575,462 Y126C probably damaging Het
Olfr395 A G 11: 73,907,435 I19T possibly damaging Het
Pdzk1 G T 3: 96,857,371 A324S probably benign Het
Pofut2 T C 10: 77,260,681 V80A possibly damaging Het
Rasip1 A G 7: 45,636,816 D821G probably damaging Het
Rnf10 T A 5: 115,251,102 K273* probably null Het
Slc4a9 A G 18: 36,539,775 M811V possibly damaging Het
Smchd1 T C 17: 71,444,020 D301G probably damaging Het
Tm9sf2 A T 14: 122,143,471 T159S probably benign Het
Tom1l1 G A 11: 90,649,861 P327S probably damaging Het
Trim3 A G 7: 105,618,482 probably benign Het
Trpm6 A C 19: 18,830,327 probably benign Het
Ttc13 A C 8: 124,688,882 L245R probably damaging Het
Txnrd1 T A 10: 82,877,284 V60E probably damaging Het
Ung T A 5: 114,136,386 D173E probably benign Het
Zbtb5 A G 4: 44,995,294 V30A probably damaging Het
Other mutations in Cry2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02167:Cry2 APN 2 92433821 missense possibly damaging 0.93
IGL02183:Cry2 APN 2 92413039 missense probably damaging 0.99
IGL02343:Cry2 APN 2 92426921 missense possibly damaging 0.90
IGL02432:Cry2 APN 2 92413667 missense probably damaging 0.99
IGL02725:Cry2 APN 2 92413260 splice site probably benign
IGL02932:Cry2 APN 2 92413117 nonsense probably null
IGL03122:Cry2 APN 2 92413295 missense probably damaging 1.00
IGL03366:Cry2 APN 2 92413715 missense probably damaging 1.00
R0679:Cry2 UTSW 2 92413715 missense probably damaging 1.00
R1325:Cry2 UTSW 2 92413770 missense probably damaging 1.00
R1862:Cry2 UTSW 2 92424566 missense probably damaging 1.00
R1891:Cry2 UTSW 2 92413640 missense possibly damaging 0.93
R2189:Cry2 UTSW 2 92411692 missense possibly damaging 0.84
R4032:Cry2 UTSW 2 92413827 missense probably benign 0.00
R4689:Cry2 UTSW 2 92424554 missense probably benign 0.38
R5130:Cry2 UTSW 2 92424599 missense probably benign 0.28
R5145:Cry2 UTSW 2 92413060 missense probably benign
R5970:Cry2 UTSW 2 92412967 missense probably benign 0.08
R6179:Cry2 UTSW 2 92413842 missense probably damaging 0.98
R7102:Cry2 UTSW 2 92413093 missense probably damaging 0.99
R7158:Cry2 UTSW 2 92413715 missense probably damaging 1.00
R7213:Cry2 UTSW 2 92413659 missense probably benign 0.00
R7257:Cry2 UTSW 2 92412981 missense possibly damaging 0.67
R7378:Cry2 UTSW 2 92413664 missense probably damaging 1.00
R7427:Cry2 UTSW 2 92413047 missense possibly damaging 0.74
R7428:Cry2 UTSW 2 92413047 missense possibly damaging 0.74
R7440:Cry2 UTSW 2 92413638 missense probably damaging 1.00
R7531:Cry2 UTSW 2 92413005 missense probably damaging 0.98
R8234:Cry2 UTSW 2 92412629 missense probably benign
R8350:Cry2 UTSW 2 92413941 missense probably benign 0.00
R8450:Cry2 UTSW 2 92413941 missense probably benign 0.00
R8496:Cry2 UTSW 2 92426939 missense probably damaging 1.00
R9172:Cry2 UTSW 2 92413648 missense probably damaging 1.00
R9283:Cry2 UTSW 2 92413904 missense probably damaging 1.00
Posted On 2014-05-07