Mutagenetix > Incidental Mutations

Incidental Mutation 'IGL01995:Cry2'

183652

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome 2 (photolyase-like)

Synonyms

Accession Numbers

Is this an essential gene?

Possibly essential (E-score: 0.604) question?

Stock #

IGL01995

Quality Score

Status

Chromosome

Chromosomal Location

92403646-92434043 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 92424632 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 126 (E126G)

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

AlphaFold

Q9R194

Predicted Effect

probably benign
Transcript: ENSMUST00000090559
AA Change: E126G

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: E126G

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000111278
AA Change: E126G

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: E126G

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125488

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144842

Coding Region Coverage

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503E14Rik	T	C	14: 44,163,845		probably benign	Het
AI661453	C	A	17: 47,468,517		probably benign	Het
Arhgap29	G	A	3: 122,014,328	V1052I	probably benign	Het
Arhgef17	A	G	7: 100,928,655	S1029P	probably benign	Het
Arpc5l	T	C	2: 39,008,214	S7P	probably damaging	Het
Asf1b	A	G	8: 83,955,904	I26V	probably benign	Het
Cep95	T	C	11: 106,806,371	L240P	probably damaging	Het
Cnmd	A	G	14: 79,642,068		probably benign	Het
Cntn3	T	A	6: 102,203,885	N783I	probably damaging	Het
Col6a2	C	A	10: 76,604,842		probably benign	Het
Col9a2	T	C	4: 121,050,410		probably null	Het
Cxcl15	T	C	5: 90,794,608	V13A	possibly damaging	Het
Dbr1	A	G	9: 99,583,899	D510G	probably benign	Het
Dct	A	G	14: 118,034,243	F392L	probably damaging	Het
Dgkz	A	G	2: 91,934,050		probably benign	Het
Dhrs7b	A	G	11: 60,830,715	I2V	probably benign	Het
Efnb3	A	T	11: 69,556,904		probably null	Het
Fbf1	T	A	11: 116,151,020	R565S	probably null	Het
Gimap5	T	C	6: 48,752,793	I99T	probably damaging	Het
Gm9912	A	G	3: 149,185,449	L16P	unknown	Het
Gpr17	A	G	18: 31,947,134	L292P	probably damaging	Het
Hdac10	C	T	15: 89,127,598	V129M	probably damaging	Het
Mfap3l	A	G	8: 60,671,464	I247V	possibly damaging	Het
Mtus1	A	T	8: 41,084,420	S86R	probably damaging	Het
Mylk3	A	G	8: 85,359,261	S55P	possibly damaging	Het
Nol6	A	T	4: 41,118,567	C745S	probably damaging	Het
Nudt13	A	T	14: 20,306,317	E103D	probably damaging	Het
Olfr1129	A	G	2: 87,575,462	Y126C	probably damaging	Het
Olfr395	A	G	11: 73,907,435	I19T	possibly damaging	Het
Pdzk1	G	T	3: 96,857,371	A324S	probably benign	Het
Pofut2	T	C	10: 77,260,681	V80A	possibly damaging	Het
Rasip1	A	G	7: 45,636,816	D821G	probably damaging	Het
Rnf10	T	A	5: 115,251,102	K273*	probably null	Het
Slc4a9	A	G	18: 36,539,775	M811V	possibly damaging	Het
Smchd1	T	C	17: 71,444,020	D301G	probably damaging	Het
Tm9sf2	A	T	14: 122,143,471	T159S	probably benign	Het
Tom1l1	G	A	11: 90,649,861	P327S	probably damaging	Het
Trim3	A	G	7: 105,618,482		probably benign	Het
Trpm6	A	C	19: 18,830,327		probably benign	Het
Ttc13	A	C	8: 124,688,882	L245R	probably damaging	Het
Txnrd1	T	A	10: 82,877,284	V60E	probably damaging	Het
Ung	T	A	5: 114,136,386	D173E	probably benign	Het
Zbtb5	A	G	4: 44,995,294	V30A	probably damaging	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02167:Cry2	APN	2	92433821	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92413039	missense	probably damaging	0.99
IGL02343:Cry2	APN	2	92426921	missense	possibly damaging	0.90
IGL02432:Cry2	APN	2	92413667	missense	probably damaging	0.99
IGL02725:Cry2	APN	2	92413260	splice site	probably benign
IGL02932:Cry2	APN	2	92413117	nonsense	probably null
IGL03122:Cry2	APN	2	92413295	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92413715	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R1325:Cry2	UTSW	2	92413770	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92424566	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92413640	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92411692	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92413827	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92424554	missense	probably benign	0.38
R5130:Cry2	UTSW	2	92424599	missense	probably benign	0.28
R5145:Cry2	UTSW	2	92413060	missense	probably benign
R5970:Cry2	UTSW	2	92412967	missense	probably benign	0.08
R6179:Cry2	UTSW	2	92413842	missense	probably damaging	0.98
R7102:Cry2	UTSW	2	92413093	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92413659	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92412981	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92413664	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92413638	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92413005	missense	probably damaging	0.98
R8234:Cry2	UTSW	2	92412629	missense	probably benign
R8350:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8450:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8496:Cry2	UTSW	2	92426939	missense	probably damaging	1.00
R9172:Cry2	UTSW	2	92413648	missense	probably damaging	1.00
R9283:Cry2	UTSW	2	92413904	missense	probably damaging	1.00

Posted On

2014-05-07