Mutagenetix > Incidental Mutations

Incidental Mutation 'R1462:Olfr829'

186324

Institutional Source

Beutler Lab

Gene Symbol

Olfr829

Ensembl Gene

ENSMUSG00000051414

Gene Name

olfactory receptor 829

Synonyms

GA_x6K02T2PVTD-12599710-12600648, MOR147-1

MMRRC Submission

039516-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.210) question?

Stock #

R1462 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

18854933-18859080 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 18857111 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 162 (M162K)

Ref Sequence

ENSEMBL: ENSMUSP00000058101 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058411] [ENSMUST00000212229]

AlphaFold

Q8VGX1

Predicted Effect

probably benign
Transcript: ENSMUST00000058411
AA Change: M162K

PolyPhen 2 Score 0.300 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000058101
Gene: ENSMUSG00000051414
AA Change: M162K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	40	317	2e-52	PFAM
Pfam:7TM_GPCR_Srsx	44	174	1e-10	PFAM
Pfam:7tm_1	50	299	1.6e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000212229
AA Change: M153K

PolyPhen 2 Score 0.255 (Sensitivity: 0.91; Specificity: 0.88)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.0%
20x: 92.0%

Validation Efficiency

99% (83/84)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930433I11Rik	C	A	7: 40,992,946	S104*	probably null	Het
A430093F15Rik	A	T	19: 10,785,481		probably benign	Het
Abca13	T	A	11: 9,483,924		probably benign	Het
Abca9	T	C	11: 110,160,516	D118G	probably benign	Het
AC239677.1	T	A	5: 25,951,625	I119F	possibly damaging	Het
Adamts16	A	G	13: 70,836,134	F137L	probably benign	Het
Adamts3	T	C	5: 89,861,349	I152V	probably benign	Het
Adcy4	T	A	14: 55,778,308	E441D	possibly damaging	Het
Adgra1	T	A	7: 139,875,829	Y458N	probably damaging	Het
Atpaf1	C	T	4: 115,784,953		probably benign	Het
Bhlhe22	C	G	3: 18,055,782	S332C	probably damaging	Het
Card19	T	A	13: 49,205,284	Q71L	probably benign	Het
Ccdc12	T	C	9: 110,656,594	L11P	probably damaging	Het
Ccdc129	A	G	6: 55,975,664	H864R	probably damaging	Het
Cdadc1	A	G	14: 59,575,858	Y367H	probably damaging	Het
Cdc5l	G	T	17: 45,408,362	Q542K	possibly damaging	Het
Cep170	T	C	1: 176,756,645	K723E	possibly damaging	Het
Cep70	A	G	9: 99,263,720	I147V	probably benign	Het
Cfap58	A	T	19: 47,962,430	H410L	probably damaging	Het
Chat	T	C	14: 32,420,778	K418R	probably damaging	Het
Cic	T	G	7: 25,271,607	D254E	probably damaging	Het
Ckap4	T	C	10: 84,527,567	E544G	probably damaging	Het
Crnkl1	C	T	2: 145,921,819	A500T	probably damaging	Het
Cyp2c38	T	C	19: 39,392,188	N418D	probably damaging	Het
Daam1	A	T	12: 71,944,142	I177L	unknown	Het
Dnah1	T	C	14: 31,268,781		probably benign	Het
Ercc5	A	G	1: 44,180,624	T1019A	probably damaging	Het
F13b	T	A	1: 139,507,636	V173E	probably damaging	Het
Fam126a	A	G	5: 23,985,732		probably benign	Het
Fam135b	A	G	15: 71,621,996		probably benign	Het
Fam20a	A	C	11: 109,677,317	F316V	probably damaging	Het
Flrt2	T	C	12: 95,779,338	V150A	probably damaging	Het
Fnta	A	C	8: 25,999,571		probably null	Het
Ghsr	A	G	3: 27,371,876	D27G	probably benign	Het
Glis3	G	T	19: 28,262,518		probably benign	Het
Gm5155	T	G	7: 17,915,591		noncoding transcript	Het
Gtpbp1	A	G	15: 79,707,885	N96D	probably damaging	Het
H1fnt	A	T	15: 98,256,573	W232R	unknown	Het
Ibtk	A	T	9: 85,724,145	I443N	probably damaging	Het
Ifi207	T	C	1: 173,724,947	H968R	probably damaging	Het
Ifit2	A	G	19: 34,573,186	D42G	probably null	Het
Il17rc	A	T	6: 113,478,989	D265V	probably damaging	Het
Ints10	G	A	8: 68,807,644		probably benign	Het
Itfg2	T	C	6: 128,424,728	D29G	probably damaging	Het
Kcng3	A	T	17: 83,631,063	C186S	probably damaging	Het
Lrrc1	A	G	9: 77,442,265	F295L	probably benign	Het
Mrps28	T	A	3: 8,900,124	H85L	possibly damaging	Het
Mtpn	T	A	6: 35,522,758	K37M	possibly damaging	Het
Mug1	C	T	6: 121,882,629	H1196Y	probably benign	Het
Mup4	T	C	4: 59,960,084	H60R	possibly damaging	Het
Musk	A	G	4: 58,286,204		probably benign	Het
Mybl2	T	C	2: 163,072,708	S249P	probably benign	Het
Naip6	A	G	13: 100,300,240	Y592H	possibly damaging	Het
Nrp1	A	G	8: 128,502,798	N919S	probably benign	Het
Nudt9	C	T	5: 104,065,038	Q326*	probably null	Het
Olfr1136	A	T	2: 87,693,376	C169S	probably damaging	Het
Olfr813	A	G	10: 129,857,231	T238A	probably damaging	Het
Olfr827	T	A	10: 130,210,723	I136F	probably benign	Het
Pcsk4	T	C	10: 80,325,981	E142G	probably damaging	Het
Pde3a	C	A	6: 141,459,834	P471T	probably benign	Het
Pign	A	T	1: 105,585,002	V652E	possibly damaging	Het
Prkcb	T	A	7: 122,582,449	M420K	probably damaging	Het
Prr14	T	A	7: 127,473,988		probably null	Het
Rchy1	T	A	5: 91,957,882	Q69L	probably damaging	Het
Sccpdh	A	C	1: 179,681,560		probably benign	Het
Sec23ip	T	G	7: 128,766,138	S625A	probably benign	Het
Smpdl3b	A	G	4: 132,746,614	S47P	probably damaging	Het
Stil	G	A	4: 115,023,964	M568I	probably benign	Het
Syt3	T	A	7: 44,396,010	V558E	probably damaging	Het
Sytl3	A	G	17: 6,706,031		probably benign	Het
Szt2	A	G	4: 118,373,967	V2533A	unknown	Het
Tenm4	A	G	7: 96,704,153	Y384C	probably damaging	Het
Tfam	T	C	10: 71,235,550	E94G	probably damaging	Het
Tmbim7	A	G	5: 3,664,304	T14A	probably damaging	Het
Tmtc2	A	T	10: 105,573,705	Y15*	probably null	Het
Uhrf1	C	T	17: 56,318,035	A526V	probably damaging	Het
Vmn2r67	T	C	7: 85,155,838	D22G	probably benign	Het
Vmn2r96	A	G	17: 18,597,398	I412M	possibly damaging	Het
Vmn2r-ps69	T	A	7: 85,310,352		noncoding transcript	Het
Wdr17	A	T	8: 54,670,328	I479K	probably damaging	Het
Wt1	T	C	2: 105,166,831	V371A	probably damaging	Het
Zfp536	G	T	7: 37,479,310	S226Y	probably damaging	Het
Zfp827	T	C	8: 79,076,479	V560A	probably benign	Het

Other mutations in Olfr829

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01142:Olfr829	APN	9	18857534	missense	probably damaging	0.97
IGL01696:Olfr829	APN	9	18857056	missense	probably benign	0.19
IGL02285:Olfr829	APN	9	18856990	missense	possibly damaging	0.87
R0133:Olfr829	UTSW	9	18856629	start codon destroyed	probably null
R0173:Olfr829	UTSW	9	18857029	missense	probably damaging	0.98
R0270:Olfr829	UTSW	9	18856831	missense	probably damaging	1.00
R0449:Olfr829	UTSW	9	18856649	missense	probably benign	0.00
R1111:Olfr829	UTSW	9	18857592	makesense	probably null
R1462:Olfr829	UTSW	9	18857111	missense	probably benign	0.30
R1845:Olfr829	UTSW	9	18857486	missense	possibly damaging	0.95
R4090:Olfr829	UTSW	9	18857102	missense	probably benign	0.00
R4096:Olfr829	UTSW	9	18856637	missense	probably benign
R4097:Olfr829	UTSW	9	18856637	missense	probably benign
R4755:Olfr829	UTSW	9	18857180	missense	probably benign	0.03
R4867:Olfr829	UTSW	9	18857566	missense	probably benign	0.00
R5084:Olfr829	UTSW	9	18857336	missense	probably benign	0.43
R7216:Olfr829	UTSW	9	18857336	missense	probably benign	0.43
R7252:Olfr829	UTSW	9	18857252	missense	probably damaging	1.00
R7300:Olfr829	UTSW	9	18857234	missense	not run
R7412:Olfr829	UTSW	9	18856789	missense	possibly damaging	0.51
R8079:Olfr829	UTSW	9	18857429	missense	possibly damaging	0.54
R9083:Olfr829	UTSW	9	18857254	missense	probably benign	0.00
X0024:Olfr829	UTSW	9	18857024	missense	probably damaging	1.00
X0057:Olfr829	UTSW	9	18856637	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GGACTCACAGATGATACCAAGCTGC -3'
(R):5'- AAGCCACAGCACTCTTCCTGGATG -3'

Sequencing Primer
(F):5'- GCACTATTCCAAAGATGCTGG -3'
(R):5'- TTGTTGAGCTGATATAGACCCC -3'

Posted On

2014-05-09