Mutagenetix > Incidental Mutations

Incidental Mutation 'R1657:Zfp985'

186452

Institutional Source

Beutler Lab

Gene Symbol

Zfp985

Ensembl Gene

ENSMUSG00000065999

Gene Name

zinc finger protein 985

Synonyms

Gm13154

MMRRC Submission

039693-MU

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.320) question?

Stock #

R1657 (G1)

Quality Score

142

Status

Not validated

Chromosome

Chromosomal Location

147553277-147585198 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 147584110 bp

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 478 (N478K)

Ref Sequence

ENSEMBL: ENSMUSP00000080438 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081742] [ENSMUST00000139784] [ENSMUST00000143885]

Predicted Effect

probably benign
Transcript: ENSMUST00000081742
AA Change: N478K

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000080438
Gene: ENSMUSG00000065999
AA Change: N478K

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART
ZnF_C2H2	238	260	8.34e-3	SMART
ZnF_C2H2	266	288	1.47e-3	SMART
ZnF_C2H2	294	316	2.36e-2	SMART
ZnF_C2H2	322	344	8.34e-3	SMART
ZnF_C2H2	350	372	7.67e-2	SMART
ZnF_C2H2	378	400	8.6e-5	SMART
ZnF_C2H2	406	428	8.6e-5	SMART
ZnF_C2H2	434	456	7.9e-4	SMART
ZnF_C2H2	462	484	1.95e-3	SMART
ZnF_C2H2	490	512	2.09e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000139784

SMART Domains

Protein: ENSMUSP00000123296
Gene: ENSMUSG00000065999

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000143885

SMART Domains

Protein: ENSMUSP00000121177
Gene: ENSMUSG00000065999

Domain	Start	End	E-Value	Type
KRAB	13	72	4.36e-15	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610009B22Rik	T	C	11: 51,685,678	Q131R	probably benign	Het
3632451O06Rik	G	C	14: 49,773,560	T230S	probably damaging	Het
Acaca	T	G	11: 84,264,084	D988E	probably benign	Het
Als2	A	G	1: 59,180,601	V1185A	probably damaging	Het
Amdhd2	A	G	17: 24,156,055	V391A	probably damaging	Het
Caprin1	A	T	2: 103,769,506	V608E	probably damaging	Het
Celsr3	T	A	9: 108,842,952	C2512*	probably null	Het
Cfl1	A	T	19: 5,493,555	R187W	probably damaging	Het
Cgnl1	C	T	9: 71,725,944	V42I	probably damaging	Het
Chd2	A	G	7: 73,480,430	Y826H	probably damaging	Het
Col9a2	C	A	4: 121,040,974	P28T	unknown	Het
Cyp3a44	G	A	5: 145,779,743	P346S	probably damaging	Het
Dact2	A	G	17: 14,197,990	V151A	probably benign	Het
Dhx29	T	C	13: 112,952,843	I716T	probably damaging	Het
Esam	T	C	9: 37,537,621	S342P	probably damaging	Het
Fam189a2	C	A	19: 23,975,635	C437F	probably damaging	Het
Fer1l4	A	G	2: 156,035,598	V1053A	possibly damaging	Het
Grk3	A	G	5: 112,966,982	F124S	probably damaging	Het
H2-DMa	G	A	17: 34,137,399		probably null	Het
Hsd3b5	T	A	3: 98,619,720	I137F	possibly damaging	Het
Itgav	A	T	2: 83,801,779	I902F	probably benign	Het
Itsn1	G	T	16: 91,909,223	C179F	probably damaging	Het
Kcnh8	G	A	17: 52,839,125	R347H	probably damaging	Het
Kif9	T	C	9: 110,489,966	M166T	possibly damaging	Het
Kmt5c	C	T	7: 4,746,454	Q324*	probably null	Het
Lcn9	G	A	2: 25,824,710	E154K	probably benign	Het
Mfge8	A	T	7: 79,141,773	L227Q	probably benign	Het
Mroh2b	A	T	15: 4,931,043	R753*	probably null	Het
Mtif2	G	A	11: 29,540,721	R475Q	probably benign	Het
Nln	C	T	13: 104,036,947	V584I	possibly damaging	Het
Nr2e3	T	C	9: 59,948,767	E129G	probably benign	Het
Ocstamp	T	C	2: 165,397,516	D250G	probably damaging	Het
Olfr1065	A	C	2: 86,445,218	L255V	probably damaging	Het
Olfr403	A	T	11: 74,195,896	H131L	probably damaging	Het
Olfr503	T	C	7: 108,545,377	I284T	possibly damaging	Het
Pld1	A	T	3: 28,071,187	I417L	probably benign	Het
Polr1a	A	T	6: 71,941,535	K692N	probably damaging	Het
Qsox2	A	T	2: 26,220,747	Y152*	probably null	Het
Rpap1	T	C	2: 119,783,778	D46G	possibly damaging	Het
Rpe65	A	G	3: 159,614,448	T246A	probably damaging	Het
Scn5a	T	C	9: 119,562,380	D82G	probably damaging	Het
Sema3d	A	G	5: 12,584,974	E669G	possibly damaging	Het
Serpinb6c	T	C	13: 33,880,226	N282S	probably benign	Het
Snap47	A	T	11: 59,428,770	S181T	probably benign	Het
Snx9	A	C	17: 5,918,436	T336P	possibly damaging	Het
Sphkap	G	A	1: 83,277,515	R838*	probably null	Het
Terb1	A	T	8: 104,488,491	D284E	possibly damaging	Het
Tmem266	C	T	9: 55,418,008	A153V	probably damaging	Het
Ttn	T	C	2: 76,742,804	E25915G	possibly damaging	Het
Tubal3	A	G	13: 3,933,011	T264A	possibly damaging	Het
Vldlr	G	A	19: 27,245,670	R747Q	probably benign	Het
Zc3h8	G	T	2: 128,929,957		probably benign	Het
Zfp184	C	T	13: 21,959,273	T383M	probably damaging	Het
Zfp455	T	C	13: 67,198,639	F38S	possibly damaging	Het
Zfp746	A	G	6: 48,082,174	V167A	possibly damaging	Het

Other mutations in Zfp985

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0098:Zfp985	UTSW	4	147577109	missense	probably damaging	0.97
R0324:Zfp985	UTSW	4	147582857	missense	probably benign	0.00
R1307:Zfp985	UTSW	4	147583247	missense	probably benign
R1594:Zfp985	UTSW	4	147583080	missense	probably benign	0.05
R1667:Zfp985	UTSW	4	147583950	missense	possibly damaging	0.84
R1761:Zfp985	UTSW	4	147584045	missense	probably benign	0.00
R1858:Zfp985	UTSW	4	147582858	missense	probably benign	0.29
R2509:Zfp985	UTSW	4	147582986	missense	possibly damaging	0.75
R2510:Zfp985	UTSW	4	147582986	missense	possibly damaging	0.75
R2847:Zfp985	UTSW	4	147583011	nonsense	probably null
R2848:Zfp985	UTSW	4	147583011	nonsense	probably null
R4245:Zfp985	UTSW	4	147582939	missense	probably damaging	0.96
R4260:Zfp985	UTSW	4	147583572	missense	probably damaging	1.00
R4434:Zfp985	UTSW	4	147583911	missense	probably benign	0.37
R4480:Zfp985	UTSW	4	147584079	missense	probably benign	0.07
R4512:Zfp985	UTSW	4	147583563	missense	probably damaging	1.00
R4514:Zfp985	UTSW	4	147583563	missense	probably damaging	1.00
R4528:Zfp985	UTSW	4	147582890	missense	possibly damaging	0.49
R4836:Zfp985	UTSW	4	147584155	missense	probably damaging	0.97
R4884:Zfp985	UTSW	4	147583344	missense	probably benign	0.04
R5054:Zfp985	UTSW	4	147582981	missense	probably damaging	0.98
R5106:Zfp985	UTSW	4	147584155	missense	probably damaging	0.97
R5205:Zfp985	UTSW	4	147582911	missense	probably damaging	1.00
R5266:Zfp985	UTSW	4	147582832	critical splice acceptor site	probably null
R5468:Zfp985	UTSW	4	147583245	missense	probably benign
R5533:Zfp985	UTSW	4	147582983	nonsense	probably null
R6282:Zfp985	UTSW	4	147583348	missense	probably benign	0.00
R6303:Zfp985	UTSW	4	147583775	missense	probably benign	0.01
R6609:Zfp985	UTSW	4	147583121	missense	probably benign
R6609:Zfp985	UTSW	4	147583667	missense	probably damaging	1.00
R6722:Zfp985	UTSW	4	147583071	missense	probably benign	0.26
R6858:Zfp985	UTSW	4	147583307	nonsense	probably null
R7064:Zfp985	UTSW	4	147583116	missense	probably benign	0.20
R7216:Zfp985	UTSW	4	147583456	missense	probably damaging	1.00
R7471:Zfp985	UTSW	4	147582931	missense	possibly damaging	0.75
R7583:Zfp985	UTSW	4	147583489	nonsense	probably null
R7685:Zfp985	UTSW	4	147582874	missense	probably benign	0.00
X0050:Zfp985	UTSW	4	147583271	frame shift	probably null

Predicted Primers

Posted On

2014-05-09