Mutagenetix > Incidental Mutation

Incidental Mutation 'R1657:Tubal3'

186481

Institutional Source

Beutler Lab

Gene Symbol

Tubal3

Ensembl Gene

ENSMUSG00000021216

Gene Name

tubulin, alpha-like 3

Synonyms

MMRRC Submission

039693-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.172) question?

Stock #

R1657 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

3918274-3935277 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 3933011 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 264 (T264A)

Ref Sequence

ENSEMBL: ENSMUSP00000021639 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021639]

AlphaFold

Q3UX10

Predicted Effect

possibly damaging
Transcript: ENSMUST00000021639
AA Change: T264A

PolyPhen 2 Score 0.871 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000021639
Gene: ENSMUSG00000021216
AA Change: T264A

Domain	Start	End	E-Value	Type
Tubulin	56	253	9.39e-66	SMART
Tubulin_C	255	400	8.93e-49	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000223035

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610009B22Rik	T	C	11: 51,685,678	Q131R	probably benign	Het
3632451O06Rik	G	C	14: 49,773,560	T230S	probably damaging	Het
Acaca	T	G	11: 84,264,084	D988E	probably benign	Het
Als2	A	G	1: 59,180,601	V1185A	probably damaging	Het
Amdhd2	A	G	17: 24,156,055	V391A	probably damaging	Het
Caprin1	A	T	2: 103,769,506	V608E	probably damaging	Het
Celsr3	T	A	9: 108,842,952	C2512*	probably null	Het
Cfl1	A	T	19: 5,493,555	R187W	probably damaging	Het
Cgnl1	C	T	9: 71,725,944	V42I	probably damaging	Het
Chd2	A	G	7: 73,480,430	Y826H	probably damaging	Het
Col9a2	C	A	4: 121,040,974	P28T	unknown	Het
Cyp3a44	G	A	5: 145,779,743	P346S	probably damaging	Het
Dact2	A	G	17: 14,197,990	V151A	probably benign	Het
Dhx29	T	C	13: 112,952,843	I716T	probably damaging	Het
Esam	T	C	9: 37,537,621	S342P	probably damaging	Het
Fam189a2	C	A	19: 23,975,635	C437F	probably damaging	Het
Fer1l4	A	G	2: 156,035,598	V1053A	possibly damaging	Het
Grk3	A	G	5: 112,966,982	F124S	probably damaging	Het
H2-DMa	G	A	17: 34,137,399		probably null	Het
Hsd3b5	T	A	3: 98,619,720	I137F	possibly damaging	Het
Itgav	A	T	2: 83,801,779	I902F	probably benign	Het
Itsn1	G	T	16: 91,909,223	C179F	probably damaging	Het
Kcnh8	G	A	17: 52,839,125	R347H	probably damaging	Het
Kif9	T	C	9: 110,489,966	M166T	possibly damaging	Het
Kmt5c	C	T	7: 4,746,454	Q324*	probably null	Het
Lcn9	G	A	2: 25,824,710	E154K	probably benign	Het
Mfge8	A	T	7: 79,141,773	L227Q	probably benign	Het
Mroh2b	A	T	15: 4,931,043	R753*	probably null	Het
Mtif2	G	A	11: 29,540,721	R475Q	probably benign	Het
Nln	C	T	13: 104,036,947	V584I	possibly damaging	Het
Nr2e3	T	C	9: 59,948,767	E129G	probably benign	Het
Ocstamp	T	C	2: 165,397,516	D250G	probably damaging	Het
Olfr1065	A	C	2: 86,445,218	L255V	probably damaging	Het
Olfr403	A	T	11: 74,195,896	H131L	probably damaging	Het
Olfr503	T	C	7: 108,545,377	I284T	possibly damaging	Het
Pld1	A	T	3: 28,071,187	I417L	probably benign	Het
Polr1a	A	T	6: 71,941,535	K692N	probably damaging	Het
Qsox2	A	T	2: 26,220,747	Y152*	probably null	Het
Rpap1	T	C	2: 119,783,778	D46G	possibly damaging	Het
Rpe65	A	G	3: 159,614,448	T246A	probably damaging	Het
Scn5a	T	C	9: 119,562,380	D82G	probably damaging	Het
Sema3d	A	G	5: 12,584,974	E669G	possibly damaging	Het
Serpinb6c	T	C	13: 33,880,226	N282S	probably benign	Het
Snap47	A	T	11: 59,428,770	S181T	probably benign	Het
Snx9	A	C	17: 5,918,436	T336P	possibly damaging	Het
Sphkap	G	A	1: 83,277,515	R838*	probably null	Het
Terb1	A	T	8: 104,488,491	D284E	possibly damaging	Het
Tmem266	C	T	9: 55,418,008	A153V	probably damaging	Het
Ttn	T	C	2: 76,742,804	E25915G	possibly damaging	Het
Vldlr	G	A	19: 27,245,670	R747Q	probably benign	Het
Zc3h8	G	T	2: 128,929,957		probably benign	Het
Zfp184	C	T	13: 21,959,273	T383M	probably damaging	Het
Zfp455	T	C	13: 67,198,639	F38S	possibly damaging	Het
Zfp746	A	G	6: 48,082,174	V167A	possibly damaging	Het
Zfp985	T	A	4: 147,584,110	N478K	probably benign	Het

Other mutations in Tubal3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00595:Tubal3	APN	13	3,933,015 (GRCm38)	missense	probably damaging	1.00
IGL02053:Tubal3	APN	13	3,933,159 (GRCm38)	missense	probably damaging	0.97
IGL02120:Tubal3	APN	13	3,930,675 (GRCm38)	missense	probably damaging	0.96
IGL02426:Tubal3	APN	13	3,932,750 (GRCm38)	missense	probably damaging	0.99
IGL02548:Tubal3	APN	13	3,930,554 (GRCm38)	missense	probably benign	0.05
IGL02981:Tubal3	APN	13	3,933,257 (GRCm38)	missense	probably benign	0.02
R0458:Tubal3	UTSW	13	3,933,137 (GRCm38)	missense	probably damaging	1.00
R1626:Tubal3	UTSW	13	3,932,841 (GRCm38)	missense	probably damaging	1.00
R2084:Tubal3	UTSW	13	3,928,192 (GRCm38)	missense	possibly damaging	0.62
R3545:Tubal3	UTSW	13	3,933,560 (GRCm38)	makesense	probably null
R3976:Tubal3	UTSW	13	3,932,946 (GRCm38)	missense	probably benign
R4700:Tubal3	UTSW	13	3,933,514 (GRCm38)	missense	probably damaging	0.99
R4722:Tubal3	UTSW	13	3,928,185 (GRCm38)	missense	probably damaging	1.00
R5617:Tubal3	UTSW	13	3,933,432 (GRCm38)	missense	probably damaging	1.00
R6474:Tubal3	UTSW	13	3,933,107 (GRCm38)	missense	probably benign	0.10
R7049:Tubal3	UTSW	13	3,932,756 (GRCm38)	missense	probably damaging	1.00
R7082:Tubal3	UTSW	13	3,933,050 (GRCm38)	missense	possibly damaging	0.56
R7273:Tubal3	UTSW	13	3,930,675 (GRCm38)	missense	probably damaging	0.96
R7586:Tubal3	UTSW	13	3,928,198 (GRCm38)	missense	possibly damaging	0.78
R8805:Tubal3	UTSW	13	3,933,293 (GRCm38)	missense	probably damaging	1.00
R8921:Tubal3	UTSW	13	3,933,428 (GRCm38)	missense	probably damaging	1.00
R9043:Tubal3	UTSW	13	3,932,708 (GRCm38)	nonsense	probably null
R9173:Tubal3	UTSW	13	3,933,050 (GRCm38)	missense	possibly damaging	0.86
R9487:Tubal3	UTSW	13	3,930,674 (GRCm38)	missense	probably benign	0.03
Z1088:Tubal3	UTSW	13	3,933,511 (GRCm38)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- GGTAGAACCGTACAATGCCATCCTC -3'
(R):5'- AGCAGCAAGCCATGTACTTCCC -3'

Sequencing Primer
(F):5'- TCCACCATAGAATACTCAGATTGTGC -3'
(R):5'- TTCCCAAGCCTGGGATCAC -3'

Posted On

2014-05-09