Mutagenetix > Incidental Mutation

Incidental Mutation 'R1660:Or5b98'

186707

Institutional Source

Beutler Lab

Gene Symbol

Or5b98

Ensembl Gene

ENSMUSG00000062892

Gene Name

olfactory receptor family 5 subfamily B member 98

Synonyms

Olfr1450, GA_x6K02T2RE5P-3283121-3284098, MOR202-33

MMRRC Submission

039696-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R1660 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

12930955-12931932 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 12931055 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 34 (I34N)

Ref Sequence

ENSEMBL: ENSMUSP00000150243 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000082006] [ENSMUST00000213587] [ENSMUST00000213925]

AlphaFold

Q8VF19

Predicted Effect

probably damaging
Transcript: ENSMUST00000082006
AA Change: I34N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000080666
Gene: ENSMUSG00000062892
AA Change: I34N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	6.8e-52	PFAM
Pfam:7TM_GPCR_Srsx	35	305	2.5e-6	PFAM
Pfam:7tm_1	41	290	2.1e-21	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213587
AA Change: I34N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000213925
AA Change: I34N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A1cf	C	A	19: 31,870,507 (GRCm39)	S3*	probably null	Het
Adgrv1	C	T	13: 81,624,750 (GRCm39)	V3740I	probably benign	Het
Aida	T	C	1: 183,079,127 (GRCm39)	F22S	probably damaging	Het
Ankrd65	A	T	4: 155,876,528 (GRCm39)	D220V	probably damaging	Het
Antxr2	A	T	5: 98,123,209 (GRCm39)	C279*	probably null	Het
Ap3b1	T	C	13: 94,545,320 (GRCm39)	V191A	probably damaging	Het
Arhgef28	T	C	13: 98,117,884 (GRCm39)	K595E	probably benign	Het
Atp12a	A	G	14: 56,608,305 (GRCm39)	T98A	probably benign	Het
Cdcp1	A	T	9: 123,014,427 (GRCm39)	S116T	probably benign	Het
Chrm1	T	C	19: 8,656,582 (GRCm39)	F429S	possibly damaging	Het
Ckap5	C	A	2: 91,393,303 (GRCm39)	Q395K	possibly damaging	Het
Cntn4	T	A	6: 106,656,258 (GRCm39)	I853K	probably benign	Het
Cyp2g1	G	A	7: 26,509,107 (GRCm39)		probably null	Het
Dhx57	C	T	17: 80,553,157 (GRCm39)	V1257I	possibly damaging	Het
Disp1	A	T	1: 182,869,306 (GRCm39)	V1038D	probably damaging	Het
Dmxl2	A	T	9: 54,358,314 (GRCm39)	S462T	possibly damaging	Het
Dnaaf10	A	T	11: 17,177,183 (GRCm39)	E180D	probably benign	Het
Exoc3l	A	G	8: 106,019,692 (GRCm39)		probably null	Het
Fam210a	T	C	18: 68,409,167 (GRCm39)	T48A	probably benign	Het
Fbxw5	G	A	2: 25,393,286 (GRCm39)		probably null	Het
Fkbp9	T	C	6: 56,850,434 (GRCm39)	C437R	probably damaging	Het
Gpc5	A	G	14: 115,636,691 (GRCm39)	K458R	probably benign	Het
Grik2	C	A	10: 49,120,439 (GRCm39)	G56*	probably null	Het
Igsf10	T	C	3: 59,238,706 (GRCm39)	T492A	probably damaging	Het
Kif1c	C	T	11: 70,619,223 (GRCm39)	L953F	probably damaging	Het
Lrrc51	T	C	7: 101,562,645 (GRCm39)	Y145C	probably damaging	Het
Lsmem1	GTACATACATACATACATACATACATACA	GTACATACATACATACATACATACATACATACA	12: 40,235,260 (GRCm39)		probably null	Het
Mapkbp1	A	T	2: 119,849,029 (GRCm39)	I682F	possibly damaging	Het
Mttp	A	T	3: 137,808,954 (GRCm39)	V718D	probably damaging	Het
Myt1l	T	A	12: 29,945,272 (GRCm39)	D1012E	unknown	Het
Nbn	G	A	4: 15,971,771 (GRCm39)	G301D	probably benign	Het
Ncstn	T	A	1: 171,894,339 (GRCm39)	S677C	possibly damaging	Het
Nod1	C	A	6: 54,921,218 (GRCm39)		probably null	Het
Nos1ap	A	G	1: 170,342,206 (GRCm39)	V52A	possibly damaging	Het
Or51f2	T	G	7: 102,526,863 (GRCm39)	Y179D	probably damaging	Het
Or52z14	T	A	7: 103,252,882 (GRCm39)	L7*	probably null	Het
Or5h23	T	A	16: 58,906,706 (GRCm39)	I47F	probably benign	Het
Phf13	T	C	4: 152,076,962 (GRCm39)	I77V	probably benign	Het
Pias2	A	G	18: 77,207,825 (GRCm39)	K230E	probably damaging	Het
Poli	A	G	18: 70,642,535 (GRCm39)	L469P	probably damaging	Het
Prag1	A	T	8: 36,607,177 (GRCm39)	T973S	possibly damaging	Het
Prpf40b	C	A	15: 99,203,442 (GRCm39)	H101Q	probably damaging	Het
Prss50	T	C	9: 110,691,557 (GRCm39)	V287A	possibly damaging	Het
Rbm25	C	T	12: 83,714,924 (GRCm39)		probably benign	Het
Rcor2	T	C	19: 7,246,337 (GRCm39)	V4A	probably damaging	Het
Rnf180	T	C	13: 105,407,499 (GRCm39)	T17A	probably benign	Het
Robo3	A	T	9: 37,340,440 (GRCm39)	V179E	probably damaging	Het
Sacs	T	A	14: 61,446,458 (GRCm39)	S2835T	probably damaging	Het
Serpinb3c	T	A	1: 107,199,432 (GRCm39)	H363L	probably damaging	Het
Setd1a	T	C	7: 127,395,841 (GRCm39)		probably benign	Het
Skp2	A	C	15: 9,125,201 (GRCm39)	V126G	probably benign	Het
Snph	G	A	2: 151,436,398 (GRCm39)	Q108*	probably null	Het
Snrpa1	T	A	7: 65,719,246 (GRCm39)	V144E	probably damaging	Het
Tifab	T	C	13: 56,324,248 (GRCm39)	E65G	probably damaging	Het
Tmem201	A	T	4: 149,804,032 (GRCm39)	Y468N	probably damaging	Het
Tpcn1	T	C	5: 120,687,580 (GRCm39)	N388S	possibly damaging	Het
Tssk4	A	G	14: 55,888,029 (GRCm39)	Q75R	probably null	Het
Tuba4a	T	C	1: 75,192,547 (GRCm39)	N356D	probably benign	Het
Ugt2b5	T	A	5: 87,287,477 (GRCm39)	D230V	probably benign	Het
Ush2a	T	C	1: 188,648,261 (GRCm39)	V4622A	probably benign	Het
Vmn2r11	T	G	5: 109,201,724 (GRCm39)	Y260S	possibly damaging	Het
Vmn2r89	C	A	14: 51,693,693 (GRCm39)	H348N	possibly damaging	Het
Vmn2r96	A	T	17: 18,817,988 (GRCm39)	M714L	probably benign	Het
Zbtb18	T	C	1: 177,275,329 (GRCm39)	S221P	probably benign	Het
Zfp418	A	G	7: 7,184,789 (GRCm39)	T251A	probably benign	Het
Zmynd15	A	G	11: 70,354,328 (GRCm39)	Y267C	probably damaging	Het

Other mutations in Or5b98

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01978:Or5b98	APN	19	12,931,406 (GRCm39)	missense	probably benign	0.00
IGL02378:Or5b98	APN	19	12,931,747 (GRCm39)	missense	probably benign	0.01
IGL02405:Or5b98	APN	19	12,931,823 (GRCm39)	missense	probably damaging	1.00
IGL02493:Or5b98	APN	19	12,931,138 (GRCm39)	missense	probably benign	0.12
IGL02496:Or5b98	APN	19	12,931,556 (GRCm39)	missense	possibly damaging	0.86
IGL02866:Or5b98	APN	19	12,931,719 (GRCm39)	missense	possibly damaging	0.81
IGL02886:Or5b98	APN	19	12,931,882 (GRCm39)	missense	probably benign	0.00
IGL03223:Or5b98	APN	19	12,931,268 (GRCm39)	missense	probably benign	0.00
IGL03286:Or5b98	APN	19	12,931,532 (GRCm39)	missense	probably benign	0.05
IGL03396:Or5b98	APN	19	12,931,184 (GRCm39)	missense	probably damaging	1.00
R0106:Or5b98	UTSW	19	12,931,720 (GRCm39)	missense	probably benign	0.10
R0106:Or5b98	UTSW	19	12,931,720 (GRCm39)	missense	probably benign	0.10
R0544:Or5b98	UTSW	19	12,931,066 (GRCm39)	missense	possibly damaging	0.79
R2020:Or5b98	UTSW	19	12,931,696 (GRCm39)	missense	possibly damaging	0.61
R4292:Or5b98	UTSW	19	12,931,520 (GRCm39)	missense	possibly damaging	0.83
R4647:Or5b98	UTSW	19	12,931,441 (GRCm39)	missense	probably benign	0.00
R5964:Or5b98	UTSW	19	12,931,895 (GRCm39)	missense	probably benign	0.03
R6398:Or5b98	UTSW	19	12,931,681 (GRCm39)	missense	probably damaging	1.00
R6681:Or5b98	UTSW	19	12,931,823 (GRCm39)	missense	probably damaging	1.00
R7129:Or5b98	UTSW	19	12,931,478 (GRCm39)	missense	possibly damaging	0.94
R7399:Or5b98	UTSW	19	12,931,811 (GRCm39)	missense	probably damaging	1.00
R7561:Or5b98	UTSW	19	12,931,403 (GRCm39)	missense	probably benign	0.00
R7692:Or5b98	UTSW	19	12,931,006 (GRCm39)	missense	possibly damaging	0.59
R8094:Or5b98	UTSW	19	12,931,366 (GRCm39)	missense	probably benign	0.00
R8258:Or5b98	UTSW	19	12,931,727 (GRCm39)	missense	possibly damaging	0.58
R8259:Or5b98	UTSW	19	12,931,727 (GRCm39)	missense	possibly damaging	0.58
R9522:Or5b98	UTSW	19	12,931,377 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCCAAGTAAGGTATGTGGTCAAACCTG -3'
(R):5'- AGCCCAACCATCACTTTTGGAGTC -3'

Sequencing Primer
(F):5'- GCCTTATGCAGTCTGGGAAAG -3'
(R):5'- CACTTTTGGAGTCACAGCAG -3'

Posted On

2014-05-09