Mutagenetix > Incidental Mutation

Incidental Mutation 'R1662:Scn9a'

186811

Institutional Source

Beutler Lab

Gene Symbol

Scn9a

Ensembl Gene

ENSMUSG00000075316

Gene Name

sodium channel, voltage-gated, type IX, alpha

Synonyms

PN1

MMRRC Submission

039698-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1662 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66310424-66465306 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 66313803 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 1972 (T1972S)

Ref Sequence

ENSEMBL: ENSMUSP00000126528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100063] [ENSMUST00000100064] [ENSMUST00000112354] [ENSMUST00000164384] [ENSMUST00000169900]

AlphaFold

Q62205

Predicted Effect

unknown
Transcript: ENSMUST00000100063
AA Change: T1963S

SMART Domains

Protein: ENSMUSP00000097641
Gene: ENSMUSG00000075316
AA Change: T1963S

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	403	9.5e-78	PFAM
coiled coil region	404	442	N/A	INTRINSIC
Pfam:DUF3451	465	685	1.3e-62	PFAM
Pfam:Ion_trans	768	957	9.9e-48	PFAM
Pfam:Na_trans_assoc	972	1191	2.9e-72	PFAM
low complexity region	1203	1214	N/A	INTRINSIC
Pfam:Ion_trans	1217	1445	2.8e-55	PFAM
PDB:1BYY\|A	1447	1499	9e-27	PDB
Pfam:Ion_trans	1538	1748	3.4e-52	PFAM
Pfam:PKD_channel	1599	1755	1.1e-7	PFAM
IQ	1877	1899	1.03e-3	SMART
low complexity region	1956	1972	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100064
AA Change: T1972S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000097642
Gene: ENSMUSG00000075316
AA Change: T1972S

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	125	412	2.2e-84	PFAM
low complexity region	433	446	N/A	INTRINSIC
Pfam:Na_trans_cytopl	483	693	7.5e-76	PFAM
Pfam:Ion_trans	742	977	4.1e-57	PFAM
Pfam:Na_trans_assoc	981	1185	1.4e-58	PFAM
Pfam:Ion_trans	1189	1466	7e-67	PFAM
Pfam:Ion_trans	1512	1769	1e-55	PFAM
Pfam:PKD_channel	1605	1763	2.6e-7	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112354
AA Change: T1961S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000107973
Gene: ENSMUSG00000075316
AA Change: T1961S

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.2e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141149

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152740

Predicted Effect

probably benign
Transcript: ENSMUST00000164384
AA Change: T1972S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000126528
Gene: ENSMUSG00000075316
AA Change: T1972S

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.1e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	694	4.2e-66	PFAM
Pfam:Ion_trans	777	966	8.8e-48	PFAM
Pfam:Na_trans_assoc	981	1200	6e-72	PFAM
low complexity region	1212	1223	N/A	INTRINSIC
Pfam:Ion_trans	1226	1454	2.5e-55	PFAM
PDB:1BYY\|A	1456	1508	6e-29	PDB
Pfam:Ion_trans	1547	1757	3e-52	PFAM
Pfam:PKD_channel	1608	1764	8.1e-8	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000169900
AA Change: T1961S

SMART Domains

Protein: ENSMUSP00000131711
Gene: ENSMUSG00000075316
AA Change: T1961S

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	3.7e-78	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal/neonatal lethality. Mice homozygous for a knock-in allele exhibit increased susceptibility to electrically induced seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5730409E04Rik	T	A	4: 126,505,475 (GRCm39)	M1K	probably null	Het
5730507C01Rik	A	C	12: 18,581,967 (GRCm39)	R119S	possibly damaging	Het
Abca1	T	A	4: 53,090,251 (GRCm39)		probably null	Het
Aff1	G	A	5: 103,988,923 (GRCm39)	G830D	probably damaging	Het
AI987944	T	C	7: 41,023,873 (GRCm39)	T369A	possibly damaging	Het
Aoah	A	G	13: 21,184,283 (GRCm39)		probably null	Het
Arhgap40	C	G	2: 158,381,190 (GRCm39)	C349W	probably damaging	Het
Atic	T	A	1: 71,615,286 (GRCm39)	D438E	probably benign	Het
Barhl2	A	T	5: 106,601,365 (GRCm39)	M338K	probably benign	Het
Btd	T	C	14: 31,388,747 (GRCm39)	V156A	probably damaging	Het
Catspere2	A	G	1: 177,874,552 (GRCm39)	K140E	unknown	Het
Ccdc82	T	C	9: 13,262,397 (GRCm39)	V319A	probably damaging	Het
Celsr1	G	T	15: 85,915,263 (GRCm39)	N903K	probably damaging	Het
Cenpf	T	A	1: 189,389,968 (GRCm39)	N1288I	probably damaging	Het
Ciao1	G	A	2: 127,086,857 (GRCm39)	T252I	probably benign	Het
Cma2	T	C	14: 56,210,573 (GRCm39)	C87R	probably damaging	Het
Cops7a	T	A	6: 124,939,401 (GRCm39)	R83W	probably damaging	Het
Cxcr6	A	T	9: 123,639,613 (GRCm39)	M205L	possibly damaging	Het
Dcc	A	G	18: 71,553,409 (GRCm39)	L749P	probably benign	Het
Dync1i2	C	T	2: 71,081,323 (GRCm39)	T484I	possibly damaging	Het
Epas1	A	T	17: 87,136,455 (GRCm39)	K742N	probably damaging	Het
Evc2	A	G	5: 37,506,094 (GRCm39)	T138A	probably benign	Het
F5	T	C	1: 164,035,457 (GRCm39)	I1877T	probably damaging	Het
Fat1	T	G	8: 45,406,201 (GRCm39)	V984G	probably benign	Het
Fat4	T	A	3: 39,034,928 (GRCm39)	V2860D	probably damaging	Het
Foxn4	C	T	5: 114,394,955 (GRCm39)	R324Q	probably benign	Het
Gapdhs	C	T	7: 30,436,427 (GRCm39)	R120H	probably damaging	Het
Gcnt3	T	C	9: 69,941,659 (GRCm39)	D303G	probably benign	Het
Gng11	A	T	6: 4,008,066 (GRCm39)	Y43F	probably benign	Het
Hectd4	A	C	5: 121,455,308 (GRCm39)	M651L	probably benign	Het
Ifngr2	T	A	16: 91,357,484 (GRCm39)	Y200N	probably benign	Het
Iqgap3	T	C	3: 88,005,708 (GRCm39)	V512A	probably benign	Het
Kdm2a	A	C	19: 4,378,240 (GRCm39)	D187E	probably damaging	Het
Klhl8	A	G	5: 104,019,911 (GRCm39)	V370A	probably damaging	Het
Kmt2a	G	A	9: 44,747,967 (GRCm39)		probably benign	Het
Krt12	C	A	11: 99,311,650 (GRCm39)	V184F	probably benign	Het
Lrrc8c	A	T	5: 105,754,623 (GRCm39)	I133F	probably benign	Het
Lsmem1	GTACATACATACATACATACATACATACA	GTACATACATACATACATACATACATACATACA	12: 40,235,260 (GRCm39)		probably null	Het
Map1a	C	G	2: 121,136,889 (GRCm39)	S2568R	possibly damaging	Het
Mbnl1	C	A	3: 60,532,593 (GRCm39)	Q301K	probably damaging	Het
Med12l	A	T	3: 59,001,038 (GRCm39)	K724N	probably damaging	Het
Mroh2a	A	C	1: 88,169,340 (GRCm39)	I672L	probably benign	Het
Mybph	C	T	1: 134,121,374 (GRCm39)	P45S	probably benign	Het
Myo15a	T	A	11: 60,392,527 (GRCm39)	S2157T	probably damaging	Het
Or14j2	G	T	17: 37,886,164 (GRCm39)	T50K	probably damaging	Het
Or1ab2	T	C	8: 72,863,623 (GRCm39)	V71A	probably benign	Het
Or2t35	T	A	14: 14,407,880 (GRCm38)	Y217*	probably null	Het
Or5h25	T	C	16: 58,930,967 (GRCm39)	E2G	probably benign	Het
Otogl	A	G	10: 107,634,218 (GRCm39)	I1419T	possibly damaging	Het
Ovol1	A	T	19: 5,601,667 (GRCm39)	F118L	probably damaging	Het
Pak5	T	C	2: 135,958,680 (GRCm39)	D136G	probably damaging	Het
Pik3r2	T	C	8: 71,223,250 (GRCm39)	Y417C	probably damaging	Het
Ppp2r2a	T	C	14: 67,254,052 (GRCm39)	N372S	probably benign	Het
Prss30	G	T	17: 24,191,806 (GRCm39)	N238K	possibly damaging	Het
Prss33	C	A	17: 24,053,785 (GRCm39)		probably null	Het
Ptpn4	T	C	1: 119,692,788 (GRCm39)	E187G	probably damaging	Het
Ptprg	A	T	14: 12,207,357 (GRCm38)	N100I	probably damaging	Het
Rbpms2	T	C	9: 65,558,324 (GRCm39)	V130A	probably benign	Het
Rdh7	A	T	10: 127,724,481 (GRCm39)	M1K	probably null	Het
Rtp3	A	C	9: 110,815,751 (GRCm39)	S205A	probably benign	Het
Ryr3	G	T	2: 112,539,618 (GRCm39)	D3207E	probably damaging	Het
Scnn1b	T	C	7: 121,501,551 (GRCm39)	V122A	probably benign	Het
Slc15a4	A	G	5: 127,686,043 (GRCm39)	L213S	probably damaging	Het
Slc27a5	T	A	7: 12,725,173 (GRCm39)	I425F	probably damaging	Het
Spata31d1b	A	G	13: 59,864,442 (GRCm39)	D530G	probably benign	Het
Tcf25	T	A	8: 124,108,289 (GRCm39)	S115T	probably benign	Het
Tet2	A	G	3: 133,172,613 (GRCm39)	L1883P	possibly damaging	Het
Trim21	T	A	7: 102,211,105 (GRCm39)	R205*	probably null	Het
Ttc23	G	T	7: 67,375,069 (GRCm39)		probably null	Het
Tut7	T	C	13: 59,947,717 (GRCm39)	E466G	possibly damaging	Het
Unc13d	T	C	11: 115,959,499 (GRCm39)	K658R	probably null	Het
Vmn1r43	A	G	6: 89,846,572 (GRCm39)	F305L	possibly damaging	Het
Vmn2r2	A	T	3: 64,024,551 (GRCm39)	C677S	probably benign	Het
Wnt5a	T	C	14: 28,240,300 (GRCm39)	M150T	probably benign	Het
Ythdc1	G	A	5: 86,975,981 (GRCm39)		probably null	Het
Zdbf2	A	T	1: 63,343,408 (GRCm39)	R596*	probably null	Het

Other mutations in Scn9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Scn9a	APN	2	66,393,945 (GRCm39)	missense	probably damaging	1.00
IGL00570:Scn9a	APN	2	66,314,486 (GRCm39)	missense	probably damaging	1.00
IGL00809:Scn9a	APN	2	66,314,279 (GRCm39)	missense	probably damaging	1.00
IGL00977:Scn9a	APN	2	66,314,645 (GRCm39)	missense	probably damaging	0.99
IGL01120:Scn9a	APN	2	66,357,316 (GRCm39)	missense	probably benign	0.00
IGL01134:Scn9a	APN	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
IGL01300:Scn9a	APN	2	66,318,397 (GRCm39)	nonsense	probably null
IGL01452:Scn9a	APN	2	66,357,416 (GRCm39)	missense	probably damaging	1.00
IGL01531:Scn9a	APN	2	66,367,722 (GRCm39)	missense	probably benign	0.11
IGL01572:Scn9a	APN	2	66,324,230 (GRCm39)	missense	probably benign	0.00
IGL01645:Scn9a	APN	2	66,317,986 (GRCm39)	missense	possibly damaging	0.62
IGL01823:Scn9a	APN	2	66,314,386 (GRCm39)	missense	probably damaging	1.00
IGL01965:Scn9a	APN	2	66,314,777 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,325,170 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,377,479 (GRCm39)	missense	probably damaging	1.00
IGL02166:Scn9a	APN	2	66,323,447 (GRCm39)	missense	possibly damaging	0.95
IGL02183:Scn9a	APN	2	66,314,955 (GRCm39)	splice site	probably benign
IGL02640:Scn9a	APN	2	66,366,440 (GRCm39)	critical splice donor site	probably null
IGL02685:Scn9a	APN	2	66,367,637 (GRCm39)	missense	probably damaging	1.00
IGL02798:Scn9a	APN	2	66,370,903 (GRCm39)	missense	possibly damaging	0.52
IGL02832:Scn9a	APN	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
IGL03008:Scn9a	APN	2	66,392,855 (GRCm39)	missense	probably damaging	1.00
IGL03270:Scn9a	APN	2	66,314,358 (GRCm39)	missense	probably damaging	1.00
IGL03408:Scn9a	APN	2	66,357,091 (GRCm39)	missense	probably benign	0.00
BB007:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
BB017:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R0039:Scn9a	UTSW	2	66,392,788 (GRCm39)	missense	probably damaging	0.98
R0173:Scn9a	UTSW	2	66,363,437 (GRCm39)	missense	probably damaging	1.00
R0323:Scn9a	UTSW	2	66,398,475 (GRCm39)	missense	probably damaging	1.00
R0344:Scn9a	UTSW	2	66,335,354 (GRCm39)	missense	probably damaging	0.99
R0421:Scn9a	UTSW	2	66,373,621 (GRCm39)	missense	probably benign
R0465:Scn9a	UTSW	2	66,357,340 (GRCm39)	missense	probably damaging	1.00
R0514:Scn9a	UTSW	2	66,314,022 (GRCm39)	missense	probably damaging	1.00
R0599:Scn9a	UTSW	2	66,357,143 (GRCm39)	missense	probably damaging	0.96
R0627:Scn9a	UTSW	2	66,367,721 (GRCm39)	missense	probably benign	0.00
R0644:Scn9a	UTSW	2	66,363,405 (GRCm39)	critical splice donor site	probably null
R0653:Scn9a	UTSW	2	66,363,721 (GRCm39)	missense	probably damaging	1.00
R0685:Scn9a	UTSW	2	66,313,843 (GRCm39)	missense	probably benign	0.02
R0718:Scn9a	UTSW	2	66,377,456 (GRCm39)	missense	probably damaging	1.00
R0827:Scn9a	UTSW	2	66,366,468 (GRCm39)	nonsense	probably null
R0890:Scn9a	UTSW	2	66,314,079 (GRCm39)	missense	probably damaging	1.00
R1139:Scn9a	UTSW	2	66,335,341 (GRCm39)	missense	probably benign	0.02
R1385:Scn9a	UTSW	2	66,393,886 (GRCm39)	missense	probably damaging	1.00
R1398:Scn9a	UTSW	2	66,314,930 (GRCm39)	missense	probably benign	0.11
R1496:Scn9a	UTSW	2	66,357,232 (GRCm39)	missense	probably benign
R1511:Scn9a	UTSW	2	66,357,157 (GRCm39)	missense	probably benign	0.01
R1517:Scn9a	UTSW	2	66,335,371 (GRCm39)	splice site	probably benign
R1564:Scn9a	UTSW	2	66,314,648 (GRCm39)	missense	probably damaging	1.00
R1634:Scn9a	UTSW	2	66,318,361 (GRCm39)	missense	probably damaging	1.00
R1695:Scn9a	UTSW	2	66,335,220 (GRCm39)	nonsense	probably null
R1709:Scn9a	UTSW	2	66,313,850 (GRCm39)	missense	probably damaging	1.00
R1741:Scn9a	UTSW	2	66,317,938 (GRCm39)	missense	probably damaging	0.99
R1755:Scn9a	UTSW	2	66,332,060 (GRCm39)	missense	probably benign	0.38
R1914:Scn9a	UTSW	2	66,396,594 (GRCm39)	missense	probably damaging	1.00
R1962:Scn9a	UTSW	2	66,314,655 (GRCm39)	missense	probably damaging	1.00
R1970:Scn9a	UTSW	2	66,345,724 (GRCm39)	missense	probably damaging	0.97
R2017:Scn9a	UTSW	2	66,345,665 (GRCm39)	missense	probably damaging	0.99
R2092:Scn9a	UTSW	2	66,363,720 (GRCm39)	missense	probably damaging	0.99
R2105:Scn9a	UTSW	2	66,398,527 (GRCm39)	missense	probably benign	0.25
R2114:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2115:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2128:Scn9a	UTSW	2	66,356,998 (GRCm39)	missense	probably damaging	1.00
R2157:Scn9a	UTSW	2	66,366,669 (GRCm39)	missense	probably damaging	1.00
R2162:Scn9a	UTSW	2	66,364,573 (GRCm39)	missense	probably damaging	0.98
R2350:Scn9a	UTSW	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
R3694:Scn9a	UTSW	2	66,392,749 (GRCm39)	missense	probably benign
R3771:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3772:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3773:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3922:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R3926:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R4258:Scn9a	UTSW	2	66,395,398 (GRCm39)	intron	probably benign
R4385:Scn9a	UTSW	2	66,314,900 (GRCm39)	missense	probably damaging	1.00
R4415:Scn9a	UTSW	2	66,357,037 (GRCm39)	missense	probably damaging	1.00
R4570:Scn9a	UTSW	2	66,313,902 (GRCm39)	missense	possibly damaging	0.85
R4682:Scn9a	UTSW	2	66,377,362 (GRCm39)	missense	probably benign
R4783:Scn9a	UTSW	2	66,370,967 (GRCm39)	missense	probably benign	0.01
R4822:Scn9a	UTSW	2	66,314,093 (GRCm39)	missense	possibly damaging	0.55
R4829:Scn9a	UTSW	2	66,382,057 (GRCm39)	missense	probably benign
R4908:Scn9a	UTSW	2	66,357,087 (GRCm39)	missense	probably benign	0.03
R4983:Scn9a	UTSW	2	66,396,614 (GRCm39)	missense	probably benign	0.02
R5047:Scn9a	UTSW	2	66,392,824 (GRCm39)	missense	probably damaging	1.00
R5100:Scn9a	UTSW	2	66,364,463 (GRCm39)	missense	probably damaging	1.00
R5140:Scn9a	UTSW	2	66,395,511 (GRCm39)	missense	possibly damaging	0.81
R5398:Scn9a	UTSW	2	66,318,387 (GRCm39)	missense	probably damaging	1.00
R5557:Scn9a	UTSW	2	66,377,447 (GRCm39)	missense	probably damaging	0.99
R5582:Scn9a	UTSW	2	66,395,373 (GRCm39)	intron	probably benign
R6108:Scn9a	UTSW	2	66,314,393 (GRCm39)	missense	probably damaging	1.00
R6115:Scn9a	UTSW	2	66,393,973 (GRCm39)	missense	possibly damaging	0.70
R6143:Scn9a	UTSW	2	66,317,868 (GRCm39)	missense	probably benign	0.00
R6261:Scn9a	UTSW	2	66,314,240 (GRCm39)	missense	probably damaging	1.00
R6335:Scn9a	UTSW	2	66,398,608 (GRCm39)	start codon destroyed	possibly damaging	0.91
R6429:Scn9a	UTSW	2	66,357,307 (GRCm39)	missense	possibly damaging	0.95
R6632:Scn9a	UTSW	2	66,313,846 (GRCm39)	missense	probably benign	0.23
R6681:Scn9a	UTSW	2	66,393,686 (GRCm39)	missense	possibly damaging	0.90
R6830:Scn9a	UTSW	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
R7102:Scn9a	UTSW	2	66,379,359 (GRCm39)	missense	probably damaging	1.00
R7186:Scn9a	UTSW	2	66,364,567 (GRCm39)	missense	probably damaging	1.00
R7243:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R7311:Scn9a	UTSW	2	66,314,748 (GRCm39)	missense	possibly damaging	0.54
R7328:Scn9a	UTSW	2	66,314,931 (GRCm39)	missense	probably benign
R7386:Scn9a	UTSW	2	66,370,894 (GRCm39)	missense	probably damaging	1.00
R7438:Scn9a	UTSW	2	66,377,531 (GRCm39)	missense	possibly damaging	0.81
R7483:Scn9a	UTSW	2	66,363,692 (GRCm39)	missense	probably damaging	0.99
R7485:Scn9a	UTSW	2	66,364,561 (GRCm39)	missense	probably damaging	1.00
R7526:Scn9a	UTSW	2	66,313,990 (GRCm39)	missense	probably benign
R7617:Scn9a	UTSW	2	66,370,893 (GRCm39)	missense	possibly damaging	0.55
R7642:Scn9a	UTSW	2	66,366,580 (GRCm39)	missense	probably benign	0.02
R7653:Scn9a	UTSW	2	66,357,424 (GRCm39)	missense	probably damaging	1.00
R7747:Scn9a	UTSW	2	66,314,642 (GRCm39)	missense	probably damaging	1.00
R7823:Scn9a	UTSW	2	66,314,135 (GRCm39)	missense	probably damaging	1.00
R7864:Scn9a	UTSW	2	66,314,904 (GRCm39)	missense	possibly damaging	0.73
R7890:Scn9a	UTSW	2	66,373,456 (GRCm39)	missense	probably benign	0.00
R7930:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R7975:Scn9a	UTSW	2	66,314,597 (GRCm39)	missense	probably damaging	1.00
R8057:Scn9a	UTSW	2	66,345,774 (GRCm39)	missense	probably benign	0.06
R8145:Scn9a	UTSW	2	66,317,754 (GRCm39)	missense	probably damaging	1.00
R8163:Scn9a	UTSW	2	66,314,745 (GRCm39)	missense	probably damaging	1.00
R8165:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R8342:Scn9a	UTSW	2	66,366,626 (GRCm39)	missense	probably benign
R8345:Scn9a	UTSW	2	66,324,966 (GRCm39)	missense	probably damaging	0.96
R8464:Scn9a	UTSW	2	66,396,625 (GRCm39)	missense	probably damaging	0.99
R8467:Scn9a	UTSW	2	66,332,015 (GRCm39)	missense	probably damaging	1.00
R8698:Scn9a	UTSW	2	66,366,628 (GRCm39)	missense	probably benign	0.00
R8810:Scn9a	UTSW	2	66,332,010 (GRCm39)	missense	probably damaging	1.00
R8822:Scn9a	UTSW	2	66,370,979 (GRCm39)	missense	probably damaging	0.99
R8829:Scn9a	UTSW	2	66,313,961 (GRCm39)	missense	probably benign
R9009:Scn9a	UTSW	2	66,338,927 (GRCm39)	missense	probably damaging	1.00
R9038:Scn9a	UTSW	2	66,325,147 (GRCm39)	missense	probably damaging	1.00
R9126:Scn9a	UTSW	2	66,314,744 (GRCm39)	missense	probably damaging	1.00
R9205:Scn9a	UTSW	2	66,363,657 (GRCm39)	missense	probably damaging	1.00
R9300:Scn9a	UTSW	2	66,335,236 (GRCm39)	missense	probably benign	0.39
R9373:Scn9a	UTSW	2	66,314,261 (GRCm39)	missense	probably benign	0.00
R9404:Scn9a	UTSW	2	66,357,040 (GRCm39)	missense	probably benign	0.02
R9443:Scn9a	UTSW	2	66,395,553 (GRCm39)	missense	probably damaging	1.00
R9590:Scn9a	UTSW	2	66,314,328 (GRCm39)	missense	probably benign	0.05
R9612:Scn9a	UTSW	2	66,363,708 (GRCm39)	missense	probably damaging	1.00
R9617:Scn9a	UTSW	2	66,392,809 (GRCm39)	missense	probably damaging	1.00
R9717:Scn9a	UTSW	2	66,357,002 (GRCm39)	missense	probably benign
X0003:Scn9a	UTSW	2	66,338,991 (GRCm39)	missense	probably benign	0.02
X0062:Scn9a	UTSW	2	66,398,421 (GRCm39)	missense	probably damaging	1.00
Z1176:Scn9a	UTSW	2	66,370,936 (GRCm39)	missense	probably benign	0.00
Z1177:Scn9a	UTSW	2	66,325,029 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- ACTGGATCAGAGCTAGTGGCTGTG -3'
(R):5'- ACGAAGTCTGGGAGAAGTTCGACC -3'

Sequencing Primer
(F):5'- CTGTGCTGCCTTTGAGAATATTTG -3'
(R):5'- GCGACTATCATTCAGCGTG -3'

Posted On

2014-05-09