Mutagenetix > Incidental Mutation

Incidental Mutation 'R1674:Naa16'

187944

Institutional Source

Beutler Lab

Gene Symbol

Naa16

Ensembl Gene

ENSMUSG00000022020

Gene Name

N(alpha)-acetyltransferase 16, NatA auxiliary subunit

Synonyms

1300019C06Rik, Narg1l

MMRRC Submission

039710-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R1674 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

79571947-79628228 bp(-) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 79624497 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000131268 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022597] [ENSMUST00000163486] [ENSMUST00000169500]

AlphaFold

Q9DBB4

Predicted Effect

probably benign
Transcript: ENSMUST00000022597
AA Change: M35K

PolyPhen 2 Score 0.025 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000022597
Gene: ENSMUSG00000022020
AA Change: M35K

Domain	Start	End	E-Value	Type
TPR	46	79	2.99e1	SMART
TPR	80	113	2.98e-3	SMART
Blast:TPR	224	257	1e-10	BLAST
TPR	374	407	9.96e0	SMART
TPR	408	441	7.47e0	SMART
low complexity region	616	633	N/A	INTRINSIC
Blast:TPR	672	705	3e-12	BLAST
low complexity region	830	841	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000163486
AA Change: M1K

PolyPhen 2 Score 0.653 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000131268
Gene: ENSMUSG00000022020
AA Change: M1K

Domain	Start	End	E-Value	Type
TPR	12	45	2.99e1	SMART
TPR	46	79	2.98e-3	SMART
Blast:TPR	190	223	3e-10	BLAST
TPR	340	373	9.96e0	SMART
TPR	374	407	7.47e0	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000165337

Predicted Effect

probably benign
Transcript: ENSMUST00000169500
AA Change: M35K

PolyPhen 2 Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000127298
Gene: ENSMUSG00000022020
AA Change: M35K

Domain	Start	End	E-Value	Type
PDB:4KVO\|D	1	46	1e-6	PDB

Meta Mutation Damage Score

0.0963

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

100% (91/91)

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579G24Rik	A	G	3: 79,538,451 (GRCm39)	T66A	probably benign	Het
5430401F13Rik	A	T	6: 131,529,766 (GRCm39)	Q120L	unknown	Het
Akirin1	A	G	4: 123,637,256 (GRCm39)	S110P	possibly damaging	Het
Ankmy2	T	A	12: 36,237,668 (GRCm39)	S256T	probably benign	Het
Areg	T	C	5: 91,291,485 (GRCm39)	F143L	probably damaging	Het
Arhgap42	A	G	9: 9,006,585 (GRCm39)	S604P	probably damaging	Het
Arid1a	A	G	4: 133,416,571 (GRCm39)	V1068A	unknown	Het
Arid4a	G	A	12: 71,122,112 (GRCm39)	S509N	probably benign	Het
Atoh1	T	G	6: 64,706,914 (GRCm39)	I203S	possibly damaging	Het
Atp5mg	T	C	9: 44,825,957 (GRCm39)	T69A	possibly damaging	Het
Baz1b	A	G	5: 135,233,965 (GRCm39)	E164G	probably damaging	Het
Baz2b	A	T	2: 59,743,336 (GRCm39)	V1545E	possibly damaging	Het
Best1	A	G	19: 9,970,590 (GRCm39)		probably null	Het
Casp8	T	A	1: 58,883,575 (GRCm39)	I314N	probably damaging	Het
Ccdc13	T	C	9: 121,638,208 (GRCm39)	T26A	probably damaging	Het
Ccr6	A	T	17: 8,475,049 (GRCm39)	I85L	probably damaging	Het
Cdh10	A	T	15: 18,985,152 (GRCm39)	N272I	probably benign	Het
Cdh10	T	A	15: 19,013,416 (GRCm39)	I672K	probably damaging	Het
Cdk17	A	T	10: 93,057,492 (GRCm39)	E163V	probably benign	Het
Chsy1	T	C	7: 65,821,411 (GRCm39)	F549L	probably damaging	Het
CN725425	T	C	15: 91,131,124 (GRCm39)	Y420H	possibly damaging	Het
Cpt1b	A	T	15: 89,306,535 (GRCm39)	M281K	possibly damaging	Het
Crlf2	A	G	5: 109,706,669 (GRCm39)		probably null	Het
Ctif	T	C	18: 75,770,251 (GRCm39)	T45A	probably benign	Het
Ddx31	T	C	2: 28,748,828 (GRCm39)	F252S	probably damaging	Het
Dennd2d	T	C	3: 106,399,833 (GRCm39)	I242T	probably benign	Het
Dennd5b	A	G	6: 148,899,782 (GRCm39)	F1205S	probably damaging	Het
Dop1a	T	C	9: 86,418,213 (GRCm39)	S1981P	probably damaging	Het
Dsg1b	A	T	18: 20,532,578 (GRCm39)	T541S	probably benign	Het
Dst	T	A	1: 34,262,876 (GRCm39)		probably null	Het
Dysf	T	C	6: 84,156,697 (GRCm39)	V1508A	probably benign	Het
Erf	A	T	7: 24,944,731 (GRCm39)	L200Q	possibly damaging	Het
Erich3	A	G	3: 154,468,260 (GRCm39)		probably benign	Het
Esrrb	C	T	12: 86,561,225 (GRCm39)	L320F	probably damaging	Het
Fap	T	A	2: 62,349,349 (GRCm39)	D508V	probably benign	Het
Fdft1	A	C	14: 63,402,034 (GRCm39)	N48K	probably benign	Het
Fdps	A	G	3: 89,008,037 (GRCm39)	V94A	probably benign	Het
Fes	A	T	7: 80,027,686 (GRCm39)	H819Q	probably benign	Het
Foxd1	G	T	13: 98,491,347 (GRCm39)	D74Y	unknown	Het
Gm136	A	T	4: 34,746,662 (GRCm39)		probably benign	Het
Gm5919	T	A	9: 83,765,338 (GRCm39)	L58*	probably null	Het
Gm6871	C	T	7: 41,223,059 (GRCm39)	V10I	possibly damaging	Het
Isy1	T	C	6: 87,811,469 (GRCm39)	R29G	probably damaging	Het
Kif16b	T	A	2: 142,554,873 (GRCm39)	K653*	probably null	Het
Kif17	A	G	4: 138,028,569 (GRCm39)	T706A	probably benign	Het
Kif18b	G	T	11: 102,803,886 (GRCm39)	P425T	probably benign	Het
Lama1	T	A	17: 68,098,239 (GRCm39)	V1812E	probably benign	Het
Lama5	A	G	2: 179,843,780 (GRCm39)	V430A	probably benign	Het
Lclat1	A	G	17: 73,546,776 (GRCm39)	E231G	probably damaging	Het
Lig4	T	C	8: 10,021,692 (GRCm39)	D696G	probably benign	Het
Mylpf	T	A	7: 126,813,309 (GRCm39)	V151E	probably damaging	Het
Ndufaf6	T	C	4: 11,070,264 (GRCm39)	K119R	probably benign	Het
Nt5c1b	T	C	12: 10,420,055 (GRCm39)		probably benign	Het
Or14a260	G	A	7: 85,984,765 (GRCm39)	P280S	probably damaging	Het
Or4a27	A	G	2: 88,559,601 (GRCm39)	V114A	probably damaging	Het
Or5t17	T	C	2: 86,832,577 (GRCm39)	V88A	probably benign	Het
Or8k33	T	A	2: 86,384,204 (GRCm39)	D88V	probably damaging	Het
Or9i16	T	A	19: 13,864,954 (GRCm39)	I207L	probably benign	Het
Otud4	A	G	8: 80,399,776 (GRCm39)	N830S	probably benign	Het
Pdcd10	A	C	3: 75,448,486 (GRCm39)	M26R	probably damaging	Het
Pitpnc1	A	G	11: 107,117,071 (GRCm39)	V223A	possibly damaging	Het
Pkp2	A	G	16: 16,058,422 (GRCm39)	D368G	possibly damaging	Het
Pla1a	A	T	16: 38,235,172 (GRCm39)	M174K	probably benign	Het
Polr2b	A	G	5: 77,474,470 (GRCm39)	K436E	possibly damaging	Het
Pwwp4a	G	T	X: 72,171,261 (GRCm39)	G218C	probably damaging	Het
Rdh16	A	G	10: 127,637,226 (GRCm39)	M54V	probably benign	Het
Sall2	C	A	14: 52,551,293 (GRCm39)	C632F	probably damaging	Het
Sart1	T	A	19: 5,435,853 (GRCm39)	I120F	probably damaging	Het
Slco1a1	A	T	6: 141,881,661 (GRCm39)	M157K	probably damaging	Het
Snapc4	T	C	2: 26,266,209 (GRCm39)	T178A	probably benign	Het
Sox6	T	C	7: 115,400,654 (GRCm39)	I63V	probably benign	Het
Spin1	T	A	13: 51,303,135 (GRCm39)	Y243N	probably damaging	Het
Stmnd1	T	C	13: 46,453,097 (GRCm39)	Y258H	possibly damaging	Het
Tex13b	A	T	X: 139,710,819 (GRCm39)	N184K	probably benign	Het
Tgm5	T	C	2: 120,902,025 (GRCm39)	T215A	possibly damaging	Het
Tnks2	A	G	19: 36,849,022 (GRCm39)	T165A	probably benign	Het
Top2a	A	T	11: 98,900,099 (GRCm39)	F667Y	probably damaging	Het
Tpo	T	C	12: 30,150,567 (GRCm39)	M438V	probably benign	Het
Tyw1	A	G	5: 130,298,169 (GRCm39)	R237G	probably benign	Het
Unc5c	G	A	3: 141,463,598 (GRCm39)	V240I	possibly damaging	Het
Unc80	A	C	1: 66,548,467 (GRCm39)	T580P	probably damaging	Het
Upp2	G	A	2: 58,680,076 (GRCm39)	E301K	probably benign	Het
Utp18	A	G	11: 93,766,879 (GRCm39)		probably null	Het
Vmn1r23	T	C	6: 57,903,046 (GRCm39)	D244G	possibly damaging	Het
Vps13c	T	A	9: 67,760,985 (GRCm39)	L51*	probably null	Het
Xpnpep3	A	G	15: 81,314,968 (GRCm39)	T223A	probably benign	Het
Zfp28	T	A	7: 6,397,942 (GRCm39)	H792Q	possibly damaging	Het
Zfp804a	A	G	2: 82,089,168 (GRCm39)	K999R	probably benign	Het
Zkscan16	G	A	4: 58,948,918 (GRCm39)	V158M	possibly damaging	Het

Other mutations in Naa16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Naa16	APN	14	79,593,169 (GRCm39)	missense	probably damaging	1.00
IGL01025:Naa16	APN	14	79,622,196 (GRCm39)	missense	probably damaging	1.00
IGL01155:Naa16	APN	14	79,622,155 (GRCm39)	missense	probably damaging	0.98
IGL01335:Naa16	APN	14	79,582,556 (GRCm39)	splice site	probably benign
IGL01981:Naa16	APN	14	79,618,956 (GRCm39)	missense	probably benign	0.05
IGL02230:Naa16	APN	14	79,614,801 (GRCm39)	splice site	probably benign
IGL02313:Naa16	APN	14	79,622,108 (GRCm39)	missense	probably damaging	1.00
IGL02418:Naa16	APN	14	79,620,806 (GRCm39)	missense	probably damaging	1.00
IGL02544:Naa16	APN	14	79,573,260 (GRCm39)	missense	probably damaging	1.00
IGL03051:Naa16	APN	14	79,606,522 (GRCm39)	missense	probably benign	0.01
IGL03064:Naa16	APN	14	79,577,068 (GRCm39)	missense	probably damaging	0.98
IGL03205:Naa16	APN	14	79,593,952 (GRCm39)	missense	possibly damaging	0.89
PIT4508001:Naa16	UTSW	14	79,606,527 (GRCm39)	missense	probably benign	0.15
R0651:Naa16	UTSW	14	79,588,832 (GRCm39)	missense	probably damaging	1.00
R1429:Naa16	UTSW	14	79,596,967 (GRCm39)	missense	probably benign	0.01
R1693:Naa16	UTSW	14	79,588,896 (GRCm39)	missense	probably damaging	1.00
R1874:Naa16	UTSW	14	79,593,183 (GRCm39)	missense	possibly damaging	0.62
R1992:Naa16	UTSW	14	79,593,931 (GRCm39)	missense	probably damaging	1.00
R2015:Naa16	UTSW	14	79,582,499 (GRCm39)	missense	probably damaging	1.00
R2391:Naa16	UTSW	14	79,607,489 (GRCm39)	missense	probably benign	0.16
R2847:Naa16	UTSW	14	79,573,323 (GRCm39)	missense	probably damaging	1.00
R2848:Naa16	UTSW	14	79,573,323 (GRCm39)	missense	probably damaging	1.00
R2877:Naa16	UTSW	14	79,580,738 (GRCm39)	missense	probably benign	0.00
R3884:Naa16	UTSW	14	79,580,702 (GRCm39)	missense	probably damaging	0.98
R4001:Naa16	UTSW	14	79,580,561 (GRCm39)	splice site	probably null
R4199:Naa16	UTSW	14	79,593,311 (GRCm39)	missense	probably damaging	1.00
R4638:Naa16	UTSW	14	79,577,473 (GRCm39)	splice site	probably null
R4676:Naa16	UTSW	14	79,573,788 (GRCm39)	unclassified	probably benign
R4690:Naa16	UTSW	14	79,582,497 (GRCm39)	missense	probably damaging	1.00
R4952:Naa16	UTSW	14	79,582,525 (GRCm39)	missense	probably damaging	1.00
R5087:Naa16	UTSW	14	79,614,855 (GRCm39)	missense	possibly damaging	0.68
R5104:Naa16	UTSW	14	79,622,140 (GRCm39)	nonsense	probably null
R5729:Naa16	UTSW	14	79,593,220 (GRCm39)	missense	probably damaging	1.00
R6178:Naa16	UTSW	14	79,620,780 (GRCm39)	missense	possibly damaging	0.93
R6960:Naa16	UTSW	14	79,596,911 (GRCm39)	missense	possibly damaging	0.65
R7794:Naa16	UTSW	14	79,614,934 (GRCm39)	missense	probably damaging	1.00
R7936:Naa16	UTSW	14	79,578,486 (GRCm39)	missense	possibly damaging	0.47
R8356:Naa16	UTSW	14	79,596,915 (GRCm39)	missense	probably benign	0.00
R8456:Naa16	UTSW	14	79,596,915 (GRCm39)	missense	probably benign	0.00
R8892:Naa16	UTSW	14	79,628,016 (GRCm39)	missense	probably benign	0.32
R8931:Naa16	UTSW	14	79,582,395 (GRCm39)	missense	probably damaging	1.00
R9010:Naa16	UTSW	14	79,607,482 (GRCm39)	missense	probably benign	0.01
R9068:Naa16	UTSW	14	79,612,289 (GRCm39)	missense	probably benign	0.18
R9360:Naa16	UTSW	14	79,593,943 (GRCm39)	missense	probably benign	0.05
R9688:Naa16	UTSW	14	79,573,309 (GRCm39)	nonsense	probably null
X0064:Naa16	UTSW	14	79,588,829 (GRCm39)	missense	probably damaging	1.00
Z1177:Naa16	UTSW	14	79,582,419 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCACTGCACATACTAGAGCATGAAA -3'
(R):5'- AGTGGAGATGCTACATGTCAAGGCATA -3'

Sequencing Primer
(F):5'- TAGCTCAACCTAAGATTCACTCG -3'
(R):5'- GGCATATATAACCGCACTTAATGCTG -3'

Posted On

2014-05-09