Mutagenetix > Incidental Mutation

Incidental Mutation 'R1676:Or1af1'

188045

Institutional Source

Beutler Lab

Gene Symbol

Or1af1

Ensembl Gene

ENSMUSG00000068947

Gene Name

olfactory receptor family 1 subfamily AF member 1

Synonyms

Olfr366, MOR138-5P, MOR138-6, GA_x6K02T2NLDC-33902472-33903401

MMRRC Submission

039712-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R1676 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

37109503-37110432 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 37109653 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 51 (R51*)

Ref Sequence

ENSEMBL: ENSMUSP00000150608 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091001] [ENSMUST00000214897]

AlphaFold

Q7TRY4

Predicted Effect

probably null
Transcript: ENSMUST00000091001
AA Change: R51*

SMART Domains

Protein: ENSMUSP00000088524
Gene: ENSMUSG00000068947
AA Change: R51*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	5e-62	PFAM
Pfam:7tm_1	41	290	3.7e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000214897
AA Change: R51*

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810021J22Rik	G	T	11: 58,771,819 (GRCm39)	A434S	possibly damaging	Het
Abhd8	T	A	8: 71,914,517 (GRCm39)	D37V	probably damaging	Het
Acvr2a	G	A	2: 48,763,095 (GRCm39)	S97N	probably benign	Het
Ampd3	T	A	7: 110,394,940 (GRCm39)	H296Q	probably damaging	Het
Arid4a	G	A	12: 71,122,112 (GRCm39)	S509N	probably benign	Het
Cap2	T	G	13: 46,791,335 (GRCm39)	H167Q	probably damaging	Het
Car8	A	G	4: 8,185,616 (GRCm39)	L180S	probably damaging	Het
Casp8	T	A	1: 58,883,575 (GRCm39)	I314N	probably damaging	Het
Cavin2	T	A	1: 51,340,330 (GRCm39)	S336T	probably benign	Het
Cdc14b	A	T	13: 64,373,416 (GRCm39)	I119N	possibly damaging	Het
Cemip	T	A	7: 83,613,246 (GRCm39)	I651F	possibly damaging	Het
Cfap57	T	A	4: 118,453,137 (GRCm39)	D522V	probably damaging	Het
Clrn3	A	T	7: 135,120,307 (GRCm39)	V93D	probably damaging	Het
Crppa	T	C	12: 36,526,720 (GRCm39)	C170R	probably benign	Het
Cyp4f39	A	G	17: 32,701,176 (GRCm39)	D222G	probably benign	Het
D5Ertd579e	A	G	5: 36,773,453 (GRCm39)	V314A	probably benign	Het
Dchs1	C	T	7: 105,404,128 (GRCm39)	V2805I	probably benign	Het
Dsp	A	T	13: 38,377,350 (GRCm39)	K1712*	probably null	Het
Emilin2	T	C	17: 71,581,085 (GRCm39)	D547G	probably benign	Het
Erbb3	T	C	10: 128,419,117 (GRCm39)	H248R	probably benign	Het
Erich3	A	G	3: 154,468,260 (GRCm39)		probably benign	Het
Fbn1	T	C	2: 125,151,701 (GRCm39)	T2518A	probably damaging	Het
Fzd2	G	A	11: 102,496,707 (GRCm39)	V384M	probably damaging	Het
Gpc5	T	A	14: 115,607,510 (GRCm39)	S371T	probably damaging	Het
Hbb-bh2	T	C	7: 103,488,362 (GRCm39)	K145R	probably null	Het
Hectd1	G	T	12: 51,791,571 (GRCm39)	P2558Q	probably damaging	Het
Hgsnat	C	T	8: 26,444,633 (GRCm39)		probably null	Het
Hirip3	T	C	7: 126,462,647 (GRCm39)		probably null	Het
Itpkc	T	C	7: 26,907,706 (GRCm39)	D666G	probably damaging	Het
Jmjd1c	T	A	10: 67,060,588 (GRCm39)	D693E	probably benign	Het
Katnbl1	T	C	2: 112,236,454 (GRCm39)	L60P	probably damaging	Het
Kcna1	T	A	6: 126,619,645 (GRCm39)	E225V	probably damaging	Het
Kcnj6	T	A	16: 94,633,443 (GRCm39)	M223L	probably damaging	Het
Kcnk7	G	T	19: 5,757,006 (GRCm39)	V332F	probably benign	Het
Kmt5a	GAA	GA	5: 124,597,948 (GRCm39)		probably null	Het
Mdga2	C	A	12: 66,615,546 (GRCm39)	G618V	probably damaging	Het
Mdga2	C	A	12: 66,615,547 (GRCm39)	G618*	probably null	Het
Morc2b	T	C	17: 33,354,955 (GRCm39)	E939G	possibly damaging	Het
Mstn	T	A	1: 53,101,224 (GRCm39)	D100E	probably benign	Het
Mthfd1l	G	A	10: 4,033,877 (GRCm39)		probably null	Het
Muc20	T	A	16: 32,614,649 (GRCm39)	T243S	probably damaging	Het
Myo1d	A	T	11: 80,575,247 (GRCm39)	N156K	probably damaging	Het
Myo7a	A	G	7: 97,748,679 (GRCm39)		probably null	Het
Naa35	A	T	13: 59,760,490 (GRCm39)	Q274L	probably damaging	Het
Ncam1	G	A	9: 49,468,472 (GRCm39)	T329I	probably damaging	Het
Nisch	T	C	14: 30,902,859 (GRCm39)		probably benign	Het
Nlrp1b	A	T	11: 71,073,637 (GRCm39)	W69R	probably benign	Het
Nrap	T	A	19: 56,323,687 (GRCm39)	H1294L	probably damaging	Het
Nsun6	T	A	2: 15,052,024 (GRCm39)	R56*	probably null	Het
Nudt1	A	G	5: 140,320,378 (GRCm39)		probably null	Het
Nxph4	T	G	10: 127,362,077 (GRCm39)	K271N	probably damaging	Het
Or4a66	T	G	2: 88,531,661 (GRCm39)	H4P	probably benign	Het
Or51e2	T	A	7: 102,391,605 (GRCm39)	I202F	probably damaging	Het
Or52a20	T	C	7: 103,366,319 (GRCm39)	W173R	probably benign	Het
Otud6b	T	A	4: 14,825,617 (GRCm39)	N71I	probably damaging	Het
Parp8	T	A	13: 117,014,064 (GRCm39)	D623V	probably damaging	Het
Pcdhb5	T	C	18: 37,453,805 (GRCm39)	S62P	probably benign	Het
Ppp1r12b	A	G	1: 134,705,190 (GRCm39)	S833P	probably damaging	Het
Ppp1r1a	T	G	15: 103,441,915 (GRCm39)	D51A	probably damaging	Het
Prag1	C	A	8: 36,570,052 (GRCm39)	P212T	probably damaging	Het
Prxl2b	C	T	4: 154,981,520 (GRCm39)	V186I	probably benign	Het
Ptdss1	A	G	13: 67,081,701 (GRCm39)	T44A	probably damaging	Het
Rab14	T	C	2: 35,076,735 (GRCm39)	H83R	possibly damaging	Het
Rapgef3	C	T	15: 97,659,063 (GRCm39)	G101E	probably benign	Het
Rimbp3	T	A	16: 17,028,977 (GRCm39)	D800E	probably benign	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Selenbp1	A	G	3: 94,851,854 (GRCm39)	D465G	probably damaging	Het
Slc28a2b	T	C	2: 122,352,340 (GRCm39)	S366P	probably damaging	Het
Slc29a1	T	C	17: 45,899,936 (GRCm39)	D251G	probably damaging	Het
Slc6a11	G	T	6: 114,224,627 (GRCm39)	V604F	probably benign	Het
Sltm	T	A	9: 70,480,929 (GRCm39)	D260E	probably damaging	Het
Spta1	G	A	1: 174,007,405 (GRCm39)	V212M	probably damaging	Het
St6gal2	T	C	17: 55,803,396 (GRCm39)		probably null	Het
Tc2n	A	G	12: 101,655,251 (GRCm39)	S235P	probably damaging	Het
Tenm3	T	A	8: 48,870,154 (GRCm39)	N213I	possibly damaging	Het
Ticam2	T	C	18: 46,693,677 (GRCm39)	T137A	probably damaging	Het
Ttn	TCCC	TCCCC	2: 76,573,251 (GRCm39)		probably null	Het
Tyk2	G	A	9: 21,026,545 (GRCm39)	Q684*	probably null	Het
Ubqln1	A	G	13: 58,327,205 (GRCm39)	F478S	possibly damaging	Het
Uckl1	G	A	2: 181,216,711 (GRCm39)	T78I	probably damaging	Het
Uhmk1	A	G	1: 170,027,581 (GRCm39)	V372A	probably damaging	Het
Unc5c	G	A	3: 141,463,598 (GRCm39)	V240I	possibly damaging	Het
Ush2a	T	C	1: 188,460,782 (GRCm39)	L2681P	probably damaging	Het
Vmn2r1	C	T	3: 63,997,603 (GRCm39)	Q420*	probably null	Het
Vmn2r101	A	T	17: 19,832,184 (GRCm39)	T727S	probably benign	Het
Vmn2r79	A	G	7: 86,651,839 (GRCm39)	T413A	probably benign	Het
Vps13c	T	C	9: 67,834,244 (GRCm39)	V1637A	probably benign	Het
Xpc	A	G	6: 91,469,929 (GRCm39)	V765A	possibly damaging	Het
Zbtb18	T	G	1: 177,274,913 (GRCm39)		probably null	Het
Zfp712	A	T	13: 67,200,400 (GRCm39)	D28E	probably benign	Het
Zfp735	A	G	11: 73,602,301 (GRCm39)	N415S	possibly damaging	Het

Other mutations in Or1af1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01691:Or1af1	APN	2	37,110,038 (GRCm39)	missense	probably damaging	1.00
IGL01925:Or1af1	APN	2	37,110,058 (GRCm39)	missense	probably benign	0.14
IGL02355:Or1af1	APN	2	37,109,681 (GRCm39)	missense	probably damaging	1.00
IGL02362:Or1af1	APN	2	37,109,681 (GRCm39)	missense	probably damaging	1.00
IGL02671:Or1af1	APN	2	37,110,257 (GRCm39)	missense	probably damaging	1.00
IGL02821:Or1af1	APN	2	37,110,112 (GRCm39)	missense	probably damaging	1.00
R0603:Or1af1	UTSW	2	37,110,118 (GRCm39)	missense	probably damaging	1.00
R0707:Or1af1	UTSW	2	37,110,208 (GRCm39)	nonsense	probably null
R1204:Or1af1	UTSW	2	37,109,651 (GRCm39)	missense	probably benign
R1457:Or1af1	UTSW	2	37,109,671 (GRCm39)	missense	possibly damaging	0.95
R1509:Or1af1	UTSW	2	37,109,966 (GRCm39)	missense	probably damaging	1.00
R1823:Or1af1	UTSW	2	37,110,344 (GRCm39)	missense	probably damaging	0.96
R2163:Or1af1	UTSW	2	37,110,089 (GRCm39)	missense	probably damaging	1.00
R2909:Or1af1	UTSW	2	37,110,188 (GRCm39)	missense	probably damaging	0.98
R3696:Or1af1	UTSW	2	37,110,188 (GRCm39)	missense	probably damaging	0.98
R3698:Or1af1	UTSW	2	37,110,188 (GRCm39)	missense	probably damaging	0.98
R4004:Or1af1	UTSW	2	37,109,960 (GRCm39)	missense	probably benign	0.00
R4655:Or1af1	UTSW	2	37,109,885 (GRCm39)	missense	probably benign	0.03
R5311:Or1af1	UTSW	2	37,109,633 (GRCm39)	missense	probably benign	0.00
R5385:Or1af1	UTSW	2	37,109,599 (GRCm39)	missense	possibly damaging	0.77
R5433:Or1af1	UTSW	2	37,109,684 (GRCm39)	missense	probably damaging	1.00
R5499:Or1af1	UTSW	2	37,109,777 (GRCm39)	missense	possibly damaging	0.81
R5707:Or1af1	UTSW	2	37,109,901 (GRCm39)	missense	probably benign	0.00
R6330:Or1af1	UTSW	2	37,110,136 (GRCm39)	missense	probably benign	0.00
R6338:Or1af1	UTSW	2	37,109,834 (GRCm39)	missense	probably damaging	1.00
R6666:Or1af1	UTSW	2	37,110,331 (GRCm39)	missense	probably damaging	1.00
R6872:Or1af1	UTSW	2	37,109,989 (GRCm39)	missense	possibly damaging	0.60
R7412:Or1af1	UTSW	2	37,109,774 (GRCm39)	missense	possibly damaging	0.48
R7789:Or1af1	UTSW	2	37,109,672 (GRCm39)	missense	probably benign	0.01
R7831:Or1af1	UTSW	2	37,109,723 (GRCm39)	missense	probably damaging	0.98
R8220:Or1af1	UTSW	2	37,109,791 (GRCm39)	missense	probably benign	0.06
R8391:Or1af1	UTSW	2	37,110,277 (GRCm39)	missense	probably damaging	1.00
R8708:Or1af1	UTSW	2	37,109,956 (GRCm39)	missense	probably damaging	1.00
R9049:Or1af1	UTSW	2	37,109,959 (GRCm39)	missense	probably damaging	0.98
R9231:Or1af1	UTSW	2	37,109,989 (GRCm39)	missense	possibly damaging	0.60
R9294:Or1af1	UTSW	2	37,110,122 (GRCm39)	missense	possibly damaging	0.63
R9471:Or1af1	UTSW	2	37,110,400 (GRCm39)	missense	probably damaging	1.00
R9595:Or1af1	UTSW	2	37,110,281 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCAATGCTCCGGGAAAACCAG -3'
(R):5'- AGGCGACCCATGAGAATTGTGTG -3'

Sequencing Primer
(F):5'- GAGCCACATGACAGAATTCCTC -3'
(R):5'- TTGCAAATAGCAACATAGCGGTC -3'

Posted On

2014-05-09