Mutagenetix > Incidental Mutation

Incidental Mutation 'R1676:Hectd1'

188104

Institutional Source

Beutler Lab

Gene Symbol

Hectd1

Ensembl Gene

ENSMUSG00000035247

Gene Name

HECT domain E3 ubiquitin protein ligase 1

Synonyms

A630086P08Rik, b2b327Clo, opm

MMRRC Submission

039712-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1676 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

51790505-51876319 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 51791571 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Glutamine at position 2558 (P2558Q)

Ref Sequence

ENSEMBL: ENSMUSP00000046766 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042052] [ENSMUST00000179265] [ENSMUST00000218820]

AlphaFold

Q69ZR2

Predicted Effect

probably damaging
Transcript: ENSMUST00000042052
AA Change: P2558Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000046766
Gene: ENSMUSG00000035247
AA Change: P2558Q

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	395	424	1.44e-1	SMART
ANK	426	455	2.81e-4	SMART
ANK	459	488	1.55e2	SMART
low complexity region	490	509	N/A	INTRINSIC
low complexity region	630	654	N/A	INTRINSIC
low complexity region	707	723	N/A	INTRINSIC
low complexity region	821	832	N/A	INTRINSIC
Pfam:Sad1_UNC	1107	1240	9.2e-27	PFAM
low complexity region	1259	1271	N/A	INTRINSIC
Pfam:MIB_HERC2	1277	1338	7.6e-27	PFAM
low complexity region	1373	1401	N/A	INTRINSIC
low complexity region	1441	1458	N/A	INTRINSIC
low complexity region	1484	1495	N/A	INTRINSIC
low complexity region	1508	1524	N/A	INTRINSIC
low complexity region	1600	1630	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
low complexity region	1674	1703	N/A	INTRINSIC
low complexity region	1745	1752	N/A	INTRINSIC
PDB:2LC3\|A	1879	1966	4e-57	PDB
low complexity region	2101	2117	N/A	INTRINSIC
HECTc	2143	2610	8.32e-76	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000158721

Predicted Effect

probably damaging
Transcript: ENSMUST00000179265
AA Change: P2566Q

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000136449
Gene: ENSMUSG00000035247
AA Change: P2566Q

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	396	425	1.44e-1	SMART
ANK	427	456	2.81e-4	SMART
ANK	460	489	1.55e2	SMART
low complexity region	491	510	N/A	INTRINSIC
low complexity region	631	655	N/A	INTRINSIC
low complexity region	708	724	N/A	INTRINSIC
low complexity region	822	833	N/A	INTRINSIC
Pfam:Sad1_UNC	1112	1245	1.3e-26	PFAM
low complexity region	1264	1276	N/A	INTRINSIC
Pfam:MIB_HERC2	1282	1341	5.3e-26	PFAM
low complexity region	1378	1406	N/A	INTRINSIC
low complexity region	1446	1463	N/A	INTRINSIC
low complexity region	1489	1500	N/A	INTRINSIC
low complexity region	1513	1529	N/A	INTRINSIC
low complexity region	1605	1635	N/A	INTRINSIC
low complexity region	1638	1656	N/A	INTRINSIC
low complexity region	1679	1708	N/A	INTRINSIC
low complexity region	1750	1757	N/A	INTRINSIC
PDB:2LC3\|A	1884	1971	3e-57	PDB
low complexity region	2106	2122	N/A	INTRINSIC
HECTc	2148	2618	4.5e-72	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000218820

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice that are homozygous for either a gene trapped or an ENU-induced allele exhibit exencephaly associated with impaired head mesenchyme development and neural tube closure, and show eye and cranial vault dysplasia. Homozygotes for another ENU-induced allele show congenital cardiovascular defects. [provided by MGI curators]

Allele List at MGI

All alleles(30) : Gene trapped(29) Chemically induced(1)

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810021J22Rik	G	T	11: 58,771,819 (GRCm39)	A434S	possibly damaging	Het
Abhd8	T	A	8: 71,914,517 (GRCm39)	D37V	probably damaging	Het
Acvr2a	G	A	2: 48,763,095 (GRCm39)	S97N	probably benign	Het
Ampd3	T	A	7: 110,394,940 (GRCm39)	H296Q	probably damaging	Het
Arid4a	G	A	12: 71,122,112 (GRCm39)	S509N	probably benign	Het
Cap2	T	G	13: 46,791,335 (GRCm39)	H167Q	probably damaging	Het
Car8	A	G	4: 8,185,616 (GRCm39)	L180S	probably damaging	Het
Casp8	T	A	1: 58,883,575 (GRCm39)	I314N	probably damaging	Het
Cavin2	T	A	1: 51,340,330 (GRCm39)	S336T	probably benign	Het
Cdc14b	A	T	13: 64,373,416 (GRCm39)	I119N	possibly damaging	Het
Cemip	T	A	7: 83,613,246 (GRCm39)	I651F	possibly damaging	Het
Cfap57	T	A	4: 118,453,137 (GRCm39)	D522V	probably damaging	Het
Clrn3	A	T	7: 135,120,307 (GRCm39)	V93D	probably damaging	Het
Crppa	T	C	12: 36,526,720 (GRCm39)	C170R	probably benign	Het
Cyp4f39	A	G	17: 32,701,176 (GRCm39)	D222G	probably benign	Het
D5Ertd579e	A	G	5: 36,773,453 (GRCm39)	V314A	probably benign	Het
Dchs1	C	T	7: 105,404,128 (GRCm39)	V2805I	probably benign	Het
Dsp	A	T	13: 38,377,350 (GRCm39)	K1712*	probably null	Het
Emilin2	T	C	17: 71,581,085 (GRCm39)	D547G	probably benign	Het
Erbb3	T	C	10: 128,419,117 (GRCm39)	H248R	probably benign	Het
Erich3	A	G	3: 154,468,260 (GRCm39)		probably benign	Het
Fbn1	T	C	2: 125,151,701 (GRCm39)	T2518A	probably damaging	Het
Fzd2	G	A	11: 102,496,707 (GRCm39)	V384M	probably damaging	Het
Gpc5	T	A	14: 115,607,510 (GRCm39)	S371T	probably damaging	Het
Hbb-bh2	T	C	7: 103,488,362 (GRCm39)	K145R	probably null	Het
Hgsnat	C	T	8: 26,444,633 (GRCm39)		probably null	Het
Hirip3	T	C	7: 126,462,647 (GRCm39)		probably null	Het
Itpkc	T	C	7: 26,907,706 (GRCm39)	D666G	probably damaging	Het
Jmjd1c	T	A	10: 67,060,588 (GRCm39)	D693E	probably benign	Het
Katnbl1	T	C	2: 112,236,454 (GRCm39)	L60P	probably damaging	Het
Kcna1	T	A	6: 126,619,645 (GRCm39)	E225V	probably damaging	Het
Kcnj6	T	A	16: 94,633,443 (GRCm39)	M223L	probably damaging	Het
Kcnk7	G	T	19: 5,757,006 (GRCm39)	V332F	probably benign	Het
Kmt5a	GAA	GA	5: 124,597,948 (GRCm39)		probably null	Het
Mdga2	C	A	12: 66,615,546 (GRCm39)	G618V	probably damaging	Het
Mdga2	C	A	12: 66,615,547 (GRCm39)	G618*	probably null	Het
Morc2b	T	C	17: 33,354,955 (GRCm39)	E939G	possibly damaging	Het
Mstn	T	A	1: 53,101,224 (GRCm39)	D100E	probably benign	Het
Mthfd1l	G	A	10: 4,033,877 (GRCm39)		probably null	Het
Muc20	T	A	16: 32,614,649 (GRCm39)	T243S	probably damaging	Het
Myo1d	A	T	11: 80,575,247 (GRCm39)	N156K	probably damaging	Het
Myo7a	A	G	7: 97,748,679 (GRCm39)		probably null	Het
Naa35	A	T	13: 59,760,490 (GRCm39)	Q274L	probably damaging	Het
Ncam1	G	A	9: 49,468,472 (GRCm39)	T329I	probably damaging	Het
Nisch	T	C	14: 30,902,859 (GRCm39)		probably benign	Het
Nlrp1b	A	T	11: 71,073,637 (GRCm39)	W69R	probably benign	Het
Nrap	T	A	19: 56,323,687 (GRCm39)	H1294L	probably damaging	Het
Nsun6	T	A	2: 15,052,024 (GRCm39)	R56*	probably null	Het
Nudt1	A	G	5: 140,320,378 (GRCm39)		probably null	Het
Nxph4	T	G	10: 127,362,077 (GRCm39)	K271N	probably damaging	Het
Or1af1	A	T	2: 37,109,653 (GRCm39)	R51*	probably null	Het
Or4a66	T	G	2: 88,531,661 (GRCm39)	H4P	probably benign	Het
Or51e2	T	A	7: 102,391,605 (GRCm39)	I202F	probably damaging	Het
Or52a20	T	C	7: 103,366,319 (GRCm39)	W173R	probably benign	Het
Otud6b	T	A	4: 14,825,617 (GRCm39)	N71I	probably damaging	Het
Parp8	T	A	13: 117,014,064 (GRCm39)	D623V	probably damaging	Het
Pcdhb5	T	C	18: 37,453,805 (GRCm39)	S62P	probably benign	Het
Ppp1r12b	A	G	1: 134,705,190 (GRCm39)	S833P	probably damaging	Het
Ppp1r1a	T	G	15: 103,441,915 (GRCm39)	D51A	probably damaging	Het
Prag1	C	A	8: 36,570,052 (GRCm39)	P212T	probably damaging	Het
Prxl2b	C	T	4: 154,981,520 (GRCm39)	V186I	probably benign	Het
Ptdss1	A	G	13: 67,081,701 (GRCm39)	T44A	probably damaging	Het
Rab14	T	C	2: 35,076,735 (GRCm39)	H83R	possibly damaging	Het
Rapgef3	C	T	15: 97,659,063 (GRCm39)	G101E	probably benign	Het
Rimbp3	T	A	16: 17,028,977 (GRCm39)	D800E	probably benign	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Selenbp1	A	G	3: 94,851,854 (GRCm39)	D465G	probably damaging	Het
Slc28a2b	T	C	2: 122,352,340 (GRCm39)	S366P	probably damaging	Het
Slc29a1	T	C	17: 45,899,936 (GRCm39)	D251G	probably damaging	Het
Slc6a11	G	T	6: 114,224,627 (GRCm39)	V604F	probably benign	Het
Sltm	T	A	9: 70,480,929 (GRCm39)	D260E	probably damaging	Het
Spta1	G	A	1: 174,007,405 (GRCm39)	V212M	probably damaging	Het
St6gal2	T	C	17: 55,803,396 (GRCm39)		probably null	Het
Tc2n	A	G	12: 101,655,251 (GRCm39)	S235P	probably damaging	Het
Tenm3	T	A	8: 48,870,154 (GRCm39)	N213I	possibly damaging	Het
Ticam2	T	C	18: 46,693,677 (GRCm39)	T137A	probably damaging	Het
Ttn	TCCC	TCCCC	2: 76,573,251 (GRCm39)		probably null	Het
Tyk2	G	A	9: 21,026,545 (GRCm39)	Q684*	probably null	Het
Ubqln1	A	G	13: 58,327,205 (GRCm39)	F478S	possibly damaging	Het
Uckl1	G	A	2: 181,216,711 (GRCm39)	T78I	probably damaging	Het
Uhmk1	A	G	1: 170,027,581 (GRCm39)	V372A	probably damaging	Het
Unc5c	G	A	3: 141,463,598 (GRCm39)	V240I	possibly damaging	Het
Ush2a	T	C	1: 188,460,782 (GRCm39)	L2681P	probably damaging	Het
Vmn2r1	C	T	3: 63,997,603 (GRCm39)	Q420*	probably null	Het
Vmn2r101	A	T	17: 19,832,184 (GRCm39)	T727S	probably benign	Het
Vmn2r79	A	G	7: 86,651,839 (GRCm39)	T413A	probably benign	Het
Vps13c	T	C	9: 67,834,244 (GRCm39)	V1637A	probably benign	Het
Xpc	A	G	6: 91,469,929 (GRCm39)	V765A	possibly damaging	Het
Zbtb18	T	G	1: 177,274,913 (GRCm39)		probably null	Het
Zfp712	A	T	13: 67,200,400 (GRCm39)	D28E	probably benign	Het
Zfp735	A	G	11: 73,602,301 (GRCm39)	N415S	possibly damaging	Het

Other mutations in Hectd1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Hectd1	APN	12	51,806,215 (GRCm39)	missense	probably benign
IGL00402:Hectd1	APN	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
IGL00419:Hectd1	APN	12	51,810,818 (GRCm39)	missense	probably damaging	0.99
IGL00518:Hectd1	APN	12	51,823,272 (GRCm39)	splice site	probably benign
IGL00565:Hectd1	APN	12	51,837,181 (GRCm39)	missense	probably damaging	0.97
IGL00574:Hectd1	APN	12	51,820,787 (GRCm39)	missense	probably benign	0.17
IGL00576:Hectd1	APN	12	51,806,092 (GRCm39)	missense	probably damaging	0.99
IGL00788:Hectd1	APN	12	51,795,571 (GRCm39)	missense	probably damaging	0.99
IGL00978:Hectd1	APN	12	51,838,173 (GRCm39)	missense	possibly damaging	0.95
IGL01328:Hectd1	APN	12	51,807,904 (GRCm39)	missense	probably damaging	1.00
IGL01337:Hectd1	APN	12	51,849,057 (GRCm39)	missense	possibly damaging	0.95
IGL01634:Hectd1	APN	12	51,850,562 (GRCm39)	missense	probably damaging	0.98
IGL01731:Hectd1	APN	12	51,849,593 (GRCm39)	missense	possibly damaging	0.59
IGL01920:Hectd1	APN	12	51,829,337 (GRCm39)	missense	probably damaging	0.99
IGL01951:Hectd1	APN	12	51,841,280 (GRCm39)	nonsense	probably null
IGL01994:Hectd1	APN	12	51,844,725 (GRCm39)	missense	probably damaging	0.99
IGL02140:Hectd1	APN	12	51,820,920 (GRCm39)	missense	probably damaging	0.99
IGL02150:Hectd1	APN	12	51,815,974 (GRCm39)	missense	probably damaging	0.97
IGL02156:Hectd1	APN	12	51,800,916 (GRCm39)	splice site	probably benign
IGL02177:Hectd1	APN	12	51,819,103 (GRCm39)	missense	probably damaging	0.99
IGL02502:Hectd1	APN	12	51,844,635 (GRCm39)	missense	possibly damaging	0.77
IGL02505:Hectd1	APN	12	51,847,496 (GRCm39)	critical splice donor site	probably null
IGL02519:Hectd1	APN	12	51,815,894 (GRCm39)	missense	probably damaging	0.99
IGL02624:Hectd1	APN	12	51,809,233 (GRCm39)	missense	possibly damaging	0.61
IGL02833:Hectd1	APN	12	51,810,864 (GRCm39)	missense	probably damaging	0.96
IGL02851:Hectd1	APN	12	51,814,423 (GRCm39)	missense	possibly damaging	0.94
IGL02866:Hectd1	APN	12	51,837,396 (GRCm39)	missense	probably damaging	1.00
IGL02981:Hectd1	APN	12	51,815,670 (GRCm39)	missense	possibly damaging	0.70
IGL02987:Hectd1	APN	12	51,791,550 (GRCm39)	missense	probably damaging	1.00
IGL02999:Hectd1	APN	12	51,874,205 (GRCm39)	missense	possibly damaging	0.77
IGL03071:Hectd1	APN	12	51,815,957 (GRCm39)	missense	probably benign	0.00
IGL03078:Hectd1	APN	12	51,849,019 (GRCm39)	missense	probably damaging	0.98
IGL03299:Hectd1	APN	12	51,847,671 (GRCm39)	splice site	probably benign
3-1:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R0039:Hectd1	UTSW	12	51,800,608 (GRCm39)	missense	possibly damaging	0.83
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0268:Hectd1	UTSW	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
R0268:Hectd1	UTSW	12	51,815,890 (GRCm39)	missense	probably damaging	0.99
R0409:Hectd1	UTSW	12	51,829,339 (GRCm39)	missense	possibly damaging	0.59
R1019:Hectd1	UTSW	12	51,795,440 (GRCm39)	missense	probably damaging	0.99
R1072:Hectd1	UTSW	12	51,807,855 (GRCm39)	missense	probably benign	0.11
R1087:Hectd1	UTSW	12	51,823,355 (GRCm39)	missense	probably damaging	0.99
R1165:Hectd1	UTSW	12	51,810,947 (GRCm39)	splice site	probably benign
R1350:Hectd1	UTSW	12	51,809,217 (GRCm39)	missense	probably benign
R1553:Hectd1	UTSW	12	51,820,661 (GRCm39)	missense	probably damaging	0.98
R1666:Hectd1	UTSW	12	51,800,607 (GRCm39)	missense	possibly damaging	0.91
R1694:Hectd1	UTSW	12	51,791,375 (GRCm39)	missense	probably damaging	1.00
R1778:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R1856:Hectd1	UTSW	12	51,791,577 (GRCm39)	missense	probably damaging	1.00
R1859:Hectd1	UTSW	12	51,853,350 (GRCm39)	missense	probably damaging	1.00
R1884:Hectd1	UTSW	12	51,847,738 (GRCm39)	missense	probably benign	0.00
R1982:Hectd1	UTSW	12	51,832,624 (GRCm39)	missense	probably damaging	0.97
R2034:Hectd1	UTSW	12	51,803,899 (GRCm39)	splice site	probably null
R2061:Hectd1	UTSW	12	51,841,227 (GRCm39)	missense	probably damaging	0.99
R2078:Hectd1	UTSW	12	51,795,325 (GRCm39)	missense	probably damaging	0.99
R2176:Hectd1	UTSW	12	51,792,277 (GRCm39)	missense	probably damaging	1.00
R2210:Hectd1	UTSW	12	51,853,245 (GRCm39)	missense	probably damaging	0.99
R2248:Hectd1	UTSW	12	51,853,254 (GRCm39)	missense	probably damaging	0.99
R2282:Hectd1	UTSW	12	51,815,791 (GRCm39)	missense	possibly damaging	0.95
R2402:Hectd1	UTSW	12	51,792,317 (GRCm39)	missense	probably benign	0.01
R3876:Hectd1	UTSW	12	51,815,513 (GRCm39)	missense	probably damaging	0.98
R4027:Hectd1	UTSW	12	51,849,219 (GRCm39)	critical splice acceptor site	probably null
R4085:Hectd1	UTSW	12	51,821,533 (GRCm39)	missense	possibly damaging	0.93
R4115:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4116:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4169:Hectd1	UTSW	12	51,837,008 (GRCm39)	missense	probably damaging	0.97
R4434:Hectd1	UTSW	12	51,798,835 (GRCm39)	missense	probably damaging	1.00
R4507:Hectd1	UTSW	12	51,837,276 (GRCm39)	missense	probably damaging	0.97
R4578:Hectd1	UTSW	12	51,798,715 (GRCm39)	missense	probably damaging	1.00
R4579:Hectd1	UTSW	12	51,791,356 (GRCm39)	missense	probably damaging	0.97
R4709:Hectd1	UTSW	12	51,834,695 (GRCm39)	missense	possibly damaging	0.94
R4812:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R4883:Hectd1	UTSW	12	51,831,030 (GRCm39)	nonsense	probably null
R4885:Hectd1	UTSW	12	51,847,505 (GRCm39)	missense	probably damaging	0.97
R4975:Hectd1	UTSW	12	51,809,280 (GRCm39)	missense	probably benign	0.02
R4983:Hectd1	UTSW	12	51,831,045 (GRCm39)	missense	probably benign	0.01
R5007:Hectd1	UTSW	12	51,849,443 (GRCm39)	missense	possibly damaging	0.95
R5046:Hectd1	UTSW	12	51,797,171 (GRCm39)	missense	probably damaging	1.00
R5062:Hectd1	UTSW	12	51,791,662 (GRCm39)	missense	probably damaging	0.98
R5164:Hectd1	UTSW	12	51,874,272 (GRCm39)	start codon destroyed	probably null	0.60
R5213:Hectd1	UTSW	12	51,849,316 (GRCm39)	critical splice donor site	probably null
R5535:Hectd1	UTSW	12	51,849,109 (GRCm39)	missense	probably damaging	0.98
R5776:Hectd1	UTSW	12	51,810,897 (GRCm39)	missense	possibly damaging	0.91
R5846:Hectd1	UTSW	12	51,820,618 (GRCm39)	missense	probably damaging	0.99
R5907:Hectd1	UTSW	12	51,845,537 (GRCm39)	missense	probably damaging	0.98
R5911:Hectd1	UTSW	12	51,849,035 (GRCm39)	missense	probably damaging	0.99
R5919:Hectd1	UTSW	12	51,815,855 (GRCm39)	missense	probably damaging	0.98
R6051:Hectd1	UTSW	12	51,800,887 (GRCm39)	missense	probably benign
R6141:Hectd1	UTSW	12	51,792,875 (GRCm39)	critical splice donor site	probably null
R6172:Hectd1	UTSW	12	51,816,065 (GRCm39)	missense	probably damaging	1.00
R6194:Hectd1	UTSW	12	51,795,228 (GRCm39)	missense	probably damaging	0.99
R6356:Hectd1	UTSW	12	51,791,402 (GRCm39)	missense	probably damaging	1.00
R6795:Hectd1	UTSW	12	51,841,270 (GRCm39)	missense	possibly damaging	0.94
R6909:Hectd1	UTSW	12	51,810,945 (GRCm39)	splice site	probably null
R6971:Hectd1	UTSW	12	51,795,526 (GRCm39)	nonsense	probably null
R7079:Hectd1	UTSW	12	51,834,638 (GRCm39)	missense	possibly damaging	0.96
R7104:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R7171:Hectd1	UTSW	12	51,806,080 (GRCm39)	missense	probably damaging	0.99
R7296:Hectd1	UTSW	12	51,832,635 (GRCm39)	missense	possibly damaging	0.73
R7346:Hectd1	UTSW	12	51,797,104 (GRCm39)	missense	probably benign
R7355:Hectd1	UTSW	12	51,838,081 (GRCm39)	missense	possibly damaging	0.72
R7468:Hectd1	UTSW	12	51,791,588 (GRCm39)	splice site	probably null
R7531:Hectd1	UTSW	12	51,853,150 (GRCm39)	missense	probably benign	0.33
R7532:Hectd1	UTSW	12	51,837,233 (GRCm39)	missense	probably damaging	0.98
R7755:Hectd1	UTSW	12	51,849,003 (GRCm39)	missense	possibly damaging	0.86
R7807:Hectd1	UTSW	12	51,792,171 (GRCm39)	missense	probably damaging	1.00
R7842:Hectd1	UTSW	12	51,819,343 (GRCm39)	missense	probably damaging	0.99
R7922:Hectd1	UTSW	12	51,836,978 (GRCm39)	nonsense	probably null
R8059:Hectd1	UTSW	12	51,837,161 (GRCm39)	missense	possibly damaging	0.53
R8085:Hectd1	UTSW	12	51,795,679 (GRCm39)	missense	probably damaging	0.97
R8145:Hectd1	UTSW	12	51,831,016 (GRCm39)	missense	possibly damaging	0.72
R8157:Hectd1	UTSW	12	51,838,073 (GRCm39)	missense	possibly damaging	0.53
R8405:Hectd1	UTSW	12	51,874,178 (GRCm39)	missense	probably benign	0.01
R8505:Hectd1	UTSW	12	51,797,145 (GRCm39)	missense	probably damaging	1.00
R8511:Hectd1	UTSW	12	51,834,654 (GRCm39)	missense	probably benign	0.01
R8697:Hectd1	UTSW	12	51,819,320 (GRCm39)	critical splice donor site	probably benign
R8725:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8727:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8911:Hectd1	UTSW	12	51,795,616 (GRCm39)	missense	probably damaging	0.99
R8983:Hectd1	UTSW	12	51,791,410 (GRCm39)	missense	probably damaging	0.97
R9037:Hectd1	UTSW	12	51,832,665 (GRCm39)	missense	possibly damaging	0.85
R9219:Hectd1	UTSW	12	51,800,612 (GRCm39)	missense	probably damaging	0.99
R9413:Hectd1	UTSW	12	51,792,880 (GRCm39)	nonsense	probably null
R9456:Hectd1	UTSW	12	51,832,584 (GRCm39)	missense	probably benign
R9513:Hectd1	UTSW	12	51,816,079 (GRCm39)	missense	possibly damaging	0.92
R9640:Hectd1	UTSW	12	51,795,197 (GRCm39)	nonsense	probably null
R9641:Hectd1	UTSW	12	51,816,047 (GRCm39)	missense	probably benign	0.00
R9713:Hectd1	UTSW	12	51,823,328 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GACACAGGTGTTGACCGATGGATAG -3'
(R):5'- AGTAGTCACCCTGGGCAACAGATG -3'

Sequencing Primer
(F):5'- TGGATAGCTCGCATCAGTAGC -3'
(R):5'- AGAAGTCCTCAGTCCTCTTAAAGTC -3'

Posted On

2014-05-09