Mutagenetix > Incidental Mutation

Incidental Mutation 'R1680:Vcan'

188445

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, DPEAAE, heart defect, Cspg2, 5430420N07Rik

MMRRC Submission

039716-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1680 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

89655312-89742509 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 89703547 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1098 (D1098G)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109544
AA Change: D1098G

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614
AA Change: D1098G

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109546
AA Change: D1098G

PolyPhen 2 Score 0.190 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: D1098G

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159910

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017N19Rik	C	G	10: 100,603,528	P187R	probably damaging	Het
Ahnak	T	A	19: 9,009,963	H2870Q	probably benign	Het
Arhgef33	C	T	17: 80,347,651	S95F	probably damaging	Het
Atp1a2	T	A	1: 172,278,954	D827V	probably damaging	Het
Bcat1	T	G	6: 145,039,628	D96A	probably damaging	Het
Birc6	A	T	17: 74,548,746	I184L	probably benign	Het
Ccdc129	C	T	6: 55,968,766	T824I	probably damaging	Het
Clca3b	A	T	3: 144,837,824	L415M	probably damaging	Het
Clstn1	T	C	4: 149,643,726	V617A	probably benign	Het
Col22a1	G	A	15: 71,799,361	A1050V	unknown	Het
Col5a3	C	T	9: 20,784,668		probably null	Het
Csmd3	G	A	15: 47,741,170	T1059I	probably damaging	Het
Dclk2	C	T	3: 86,805,639	R503Q	possibly damaging	Het
Dnm3	A	G	1: 162,010,976	V272A	probably benign	Het
Dnmt3a	A	G	12: 3,873,361	Q187R	probably damaging	Het
Dpp9	A	G	17: 56,190,103	Y710H	probably benign	Het
Eef1a2	A	T	2: 181,152,941	M155K	possibly damaging	Het
Entpd8	G	A	2: 25,084,024	C331Y	probably damaging	Het
Erc1	A	C	6: 119,575,761	L1072R	probably damaging	Het
Fam160b2	T	C	14: 70,586,851	Y482C	probably damaging	Het
Gtf3c2	A	G	5: 31,173,868	S155P	probably damaging	Het
Gucy2c	A	G	6: 136,722,493	S617P	probably damaging	Het
Ice1	T	C	13: 70,605,448	R840G	probably benign	Het
Il1rl2	T	C	1: 40,351,793	Y299H	possibly damaging	Het
Ints7	T	G	1: 191,621,162		probably null	Het
Ireb2	C	T	9: 54,881,518	T92I	probably damaging	Het
Kcnj8	A	T	6: 142,570,189	L64*	probably null	Het
Mapk8ip3	A	G	17: 24,901,011	V983A	probably damaging	Het
Mertk	A	G	2: 128,801,636	D985G	probably benign	Het
Mical3	A	T	6: 120,959,643	S1307R	probably benign	Het
Ncaph2	A	G	15: 89,364,622	D222G	probably benign	Het
Nf1	C	A	11: 79,550,998	S295*	probably null	Het
Nlrp12	T	A	7: 3,241,174	D236V	probably damaging	Het
Npnt	A	G	3: 132,906,802	V74A	probably benign	Het
Oasl1	A	G	5: 114,935,944	D304G	probably damaging	Het
Olfr1098	C	T	2: 86,923,161	V124I	probably benign	Het
Olfr556	A	G	7: 102,670,733	D271G	possibly damaging	Het
Olfr834	A	G	9: 18,988,516	H176R	possibly damaging	Het
Olfr936	T	C	9: 39,047,000	I140V	probably benign	Het
Patz1	A	G	11: 3,307,812	K604E	probably damaging	Het
Pcsk6	T	A	7: 66,035,250	V793E	probably benign	Het
Pla2g4d	C	T	2: 120,277,750		probably null	Het
Plxnc1	A	C	10: 94,841,551	L938R	probably benign	Het
Pou4f2	G	T	8: 78,434,831	A381D	probably damaging	Het
Prdm4	A	G	10: 85,899,223	L685P	possibly damaging	Het
Pxn	T	A	5: 115,552,147	V383E	probably damaging	Het
Rbl1	A	G	2: 157,174,783	L632P	probably damaging	Het
Rnf135	T	A	11: 80,196,881	S219T	possibly damaging	Het
Sdk2	T	C	11: 113,791,436	D2039G	possibly damaging	Het
Sdk2	C	T	11: 113,838,646		silent	Het
Slc5a6	A	G	5: 31,042,644	Y131H	probably damaging	Het
Slc9a1	T	C	4: 133,418,080	I492T	probably damaging	Het
Soga3	A	T	10: 29,196,839	Q709L	probably damaging	Het
Spag5	A	G	11: 78,320,616	K993E	probably damaging	Het
Sptbn1	T	G	11: 30,159,371	I75L	possibly damaging	Het
Syngap1	T	C	17: 26,952,579	S46P	possibly damaging	Het
Tfcp2l1	T	A	1: 118,675,605	F458I	probably damaging	Het
Tmem67	A	G	4: 12,087,840	V102A	probably benign	Het
Tomm70a	T	C	16: 57,121,961	S34P	unknown	Het
Txlnb	G	T	10: 17,843,233	G604V	probably benign	Het
Ube2q1	A	G	3: 89,776,176	T143A	probably benign	Het
Unc80	C	T	1: 66,503,669	R361*	probably null	Het
Vmn1r176	T	A	7: 23,835,381	T116S	probably damaging	Het
Wdr81	C	T	11: 75,454,423	R6K	probably benign	Het
Zan	T	A	5: 137,403,050	T4136S	unknown	Het
Zbtb14	C	A	17: 69,388,502	F398L	probably damaging	Het
Zfp606	A	T	7: 12,493,971	H615L	probably damaging	Het
Zp3r	A	T	1: 130,582,880	N433K	probably benign	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,704,702 (GRCm38)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,692,319 (GRCm38)	missense	probably benign
IGL00504:Vcan	APN	13	89,691,275 (GRCm38)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,688,979 (GRCm38)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,703,726 (GRCm38)	missense	probably benign
IGL00743:Vcan	APN	13	89,725,306 (GRCm38)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,662,052 (GRCm38)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,679,958 (GRCm38)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,691,668 (GRCm38)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,703,943 (GRCm38)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,704,169 (GRCm38)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,688,675 (GRCm38)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,690,586 (GRCm38)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,689,745 (GRCm38)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,725,388 (GRCm38)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,689,205 (GRCm38)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,689,359 (GRCm38)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,684,438 (GRCm38)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,725,565 (GRCm38)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,684,493 (GRCm38)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,703,077 (GRCm38)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,704,849 (GRCm38)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,690,657 (GRCm38)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,688,623 (GRCm38)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,703,275 (GRCm38)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,661,932 (GRCm38)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,705,006 (GRCm38)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,661,985 (GRCm38)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,678,073 (GRCm38)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,691,261 (GRCm38)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,712,191 (GRCm38)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,691,275 (GRCm38)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,703,546 (GRCm38)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,703,199 (GRCm38)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,678,145 (GRCm38)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,704,660 (GRCm38)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,703,772 (GRCm38)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,731,464 (GRCm38)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,712,253 (GRCm38)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,712,267 (GRCm38)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,704,706 (GRCm38)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,679,822 (GRCm38)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,704,953 (GRCm38)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,685,077 (GRCm38)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,692,410 (GRCm38)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,704,303 (GRCm38)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,679,794 (GRCm38)	splice site	probably null
R1219:Vcan	UTSW	13	89,679,904 (GRCm38)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,657,556 (GRCm38)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,692,956 (GRCm38)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,689,661 (GRCm38)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,703,667 (GRCm38)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,661,946 (GRCm38)	missense	probably damaging	1.00
R1694:Vcan	UTSW	13	89,688,483 (GRCm38)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,721,775 (GRCm38)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,704,735 (GRCm38)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,691,681 (GRCm38)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,705,212 (GRCm38)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,705,392 (GRCm38)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,690,871 (GRCm38)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,693,015 (GRCm38)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,705,534 (GRCm38)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,702,926 (GRCm38)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,703,424 (GRCm38)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,692,742 (GRCm38)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,689,038 (GRCm38)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,690,985 (GRCm38)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,689,737 (GRCm38)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,703,529 (GRCm38)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,703,410 (GRCm38)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,689,449 (GRCm38)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,704,675 (GRCm38)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,704,237 (GRCm38)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,703,301 (GRCm38)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,679,887 (GRCm38)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,685,158 (GRCm38)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,725,547 (GRCm38)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,725,486 (GRCm38)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,704,028 (GRCm38)	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89,679,934 (GRCm38)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,705,789 (GRCm38)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,704,739 (GRCm38)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,688,842 (GRCm38)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,703,977 (GRCm38)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,657,472 (GRCm38)	intron	probably benign
R5124:Vcan	UTSW	13	89,725,517 (GRCm38)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,690,240 (GRCm38)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,690,872 (GRCm38)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,692,532 (GRCm38)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,691,600 (GRCm38)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,690,286 (GRCm38)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,691,810 (GRCm38)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,703,112 (GRCm38)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,688,671 (GRCm38)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,691,503 (GRCm38)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,691,135 (GRCm38)	frame shift	probably null
R5687:Vcan	UTSW	13	89,678,134 (GRCm38)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,679,950 (GRCm38)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,703,952 (GRCm38)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,692,691 (GRCm38)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,657,536 (GRCm38)	nonsense	probably null
R6135:Vcan	UTSW	13	89,689,926 (GRCm38)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,691,220 (GRCm38)	nonsense	probably null
R6280:Vcan	UTSW	13	89,725,373 (GRCm38)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,691,597 (GRCm38)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,704,832 (GRCm38)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,690,687 (GRCm38)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,704,731 (GRCm38)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,705,182 (GRCm38)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,705,125 (GRCm38)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,712,381 (GRCm38)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,689,431 (GRCm38)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,678,133 (GRCm38)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,679,956 (GRCm38)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,693,407 (GRCm38)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,690,618 (GRCm38)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,690,241 (GRCm38)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,705,686 (GRCm38)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,689,110 (GRCm38)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,725,591 (GRCm38)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,688,936 (GRCm38)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,705,270 (GRCm38)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,731,457 (GRCm38)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,721,686 (GRCm38)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,705,161 (GRCm38)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,692,733 (GRCm38)	nonsense	probably null
R7299:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7301:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7425:Vcan	UTSW	13	89,689,832 (GRCm38)	missense	probably damaging	0.99
R7514:Vcan	UTSW	13	89,704,118 (GRCm38)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,692,458 (GRCm38)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,692,223 (GRCm38)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,691,789 (GRCm38)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,704,619 (GRCm38)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,689,323 (GRCm38)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,692,937 (GRCm38)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,691,858 (GRCm38)	missense	probably benign
R7998:Vcan	UTSW	13	89,704,327 (GRCm38)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,704,360 (GRCm38)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,657,290 (GRCm38)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,703,320 (GRCm38)	nonsense	probably null
R8103:Vcan	UTSW	13	89,657,658 (GRCm38)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,704,254 (GRCm38)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,704,987 (GRCm38)	nonsense	probably null
R8166:Vcan	UTSW	13	89,692,736 (GRCm38)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,704,970 (GRCm38)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,704,335 (GRCm38)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,688,743 (GRCm38)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,691,098 (GRCm38)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,692,320 (GRCm38)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,692,111 (GRCm38)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,704,907 (GRCm38)	missense	probably benign
R9049:Vcan	UTSW	13	89,678,105 (GRCm38)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,691,027 (GRCm38)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,704,525 (GRCm38)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,679,931 (GRCm38)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,690,496 (GRCm38)	nonsense	probably null
R9259:Vcan	UTSW	13	89,690,870 (GRCm38)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,689,333 (GRCm38)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,703,412 (GRCm38)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,693,009 (GRCm38)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,691,540 (GRCm38)	missense
R9579:Vcan	UTSW	13	89,689,594 (GRCm38)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,705,372 (GRCm38)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,692,962 (GRCm38)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,691,741 (GRCm38)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,691,128 (GRCm38)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,689,811 (GRCm38)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,692,493 (GRCm38)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,705,749 (GRCm38)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,692,571 (GRCm38)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,704,073 (GRCm38)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,703,788 (GRCm38)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,703,524 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CACTGGACTCTTCCTCAAAGACACG -3'
(R):5'- AACTCCAGGGAGCCCATTTCCTAC -3'

Sequencing Primer
(F):5'- CTCTGGGTTCAAATAACCCTGAG -3'
(R):5'- GGGAGCCCATTTCCTACTTTCAG -3'

Posted On

2014-05-09