Incidental Mutation 'R1405:Faah'
Institutional Source Beutler Lab
Gene Symbol Faah
Ensembl Gene ENSMUSG00000034171
Gene Namefatty acid amide hydrolase
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.099) question?
Stock #R1405 (G1)
Quality Score225
Status Not validated
Chromosomal Location115967145-116017926 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to A at 116001148 bp
Amino Acid Change Proline to Serine at position 411 (P411S)
Ref Sequence ENSEMBL: ENSMUSP00000041543 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000049095]
Predicted Effect probably damaging
Transcript: ENSMUST00000049095
AA Change: P411S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000041543
Gene: ENSMUSG00000034171
AA Change: P411S

transmembrane domain 7 29 N/A INTRINSIC
low complexity region 74 87 N/A INTRINSIC
Pfam:Amidase 95 562 1.4e-122 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000125761
Predicted Effect noncoding transcript
Transcript: ENSMUST00000133225
Predicted Effect noncoding transcript
Transcript: ENSMUST00000143724
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150614
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150668
Predicted Effect noncoding transcript
Transcript: ENSMUST00000154249
Predicted Effect noncoding transcript
Transcript: ENSMUST00000156126
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.4%
  • 10x: 96.5%
  • 20x: 93.2%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a null allele show high brain anandamide (AEA) levels, reduced pain sensation, altered behavioral responses to AEA, and sex-specific changes in ethanol intake and sensitivity. Homozygotes for the C385A variant show enhanced cued fear extinction and reduced anxiety-like behavior. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca1 G A 4: 53,059,253 probably benign Het
AC163020.1 A G 7: 47,309,557 S229P possibly damaging Het
Arap1 G A 7: 101,398,436 probably null Het
Asb8 G A 15: 98,141,367 H51Y possibly damaging Het
Capn10 T G 1: 92,945,022 V490G probably benign Het
Ccdc138 T A 10: 58,545,117 probably benign Het
Ccdc146 G A 5: 21,399,732 S36L probably benign Het
Celsr1 C T 15: 85,905,434 probably null Het
Clvs2 C A 10: 33,513,260 *328L probably null Het
Cul9 C G 17: 46,522,175 A1326P probably damaging Het
D6Wsu163e G A 6: 126,974,483 probably benign Het
Dstn A G 2: 143,938,436 K19E probably damaging Het
Ehmt2 T A 17: 34,906,577 H134Q probably benign Het
Fam19a5 C T 15: 87,681,477 probably benign Het
Fn1 A G 1: 71,642,078 F364L probably damaging Het
Gm14124 T A 2: 150,267,700 Y103* probably null Het
Gm5828 T C 1: 16,769,544 noncoding transcript Het
Gmnc A T 16: 26,960,446 N270K possibly damaging Het
Grip2 A T 6: 91,788,152 probably null Het
Hmg20a A T 9: 56,477,303 Q119L possibly damaging Het
Ipo7 T C 7: 110,029,841 I106T probably benign Het
Ipo7 C T 7: 110,039,249 P241L probably damaging Het
Katnb1 T C 8: 95,098,173 Y574H probably damaging Het
Larp6 A C 9: 60,737,566 M330L probably benign Het
Lrrc8e T C 8: 4,231,754 Y30H probably damaging Het
Nav3 A T 10: 109,770,333 probably benign Het
Nop56 T C 2: 130,277,948 V420A probably benign Het
Nrg1 T C 8: 31,917,827 D126G probably benign Het
Prdm1 T A 10: 44,439,965 N725I probably damaging Het
Prl3a1 A G 13: 27,275,068 probably null Het
Psmd2 T C 16: 20,652,284 L59P possibly damaging Het
Ptgdr2 T C 19: 10,941,031 V304A probably benign Het
Ptp4a2 T A 4: 129,845,058 probably benign Het
Rasa3 A G 8: 13,588,027 V339A possibly damaging Het
Sec24c G A 14: 20,692,525 probably null Het
Serpinb9e A G 13: 33,260,026 D343G probably benign Het
Sptbn5 A T 2: 120,050,616 noncoding transcript Het
Stab1 A C 14: 31,149,001 V1297G probably benign Het
Stk4 C T 2: 164,100,528 T360M probably benign Het
Tmprss2 G A 16: 97,596,805 T57I probably benign Het
Tnrc6a A G 7: 123,171,078 D697G probably damaging Het
Vwa5b2 T A 16: 20,604,316 D1021E probably benign Het
Wdr46 C A 17: 33,949,083 P543Q probably damaging Het
Zfp287 T A 11: 62,728,311 D119V probably damaging Het
Zxdc A G 6: 90,384,243 S737G possibly damaging Het
Other mutations in Faah
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00679:Faah APN 4 116008283 missense possibly damaging 0.85
IGL03355:Faah APN 4 116002528 missense probably benign 0.28
R0013:Faah UTSW 4 116004391 missense probably damaging 1.00
R0387:Faah UTSW 4 116005692 nonsense probably null
R0727:Faah UTSW 4 116005060 missense probably damaging 1.00
R1405:Faah UTSW 4 116001148 missense probably damaging 1.00
R1465:Faah UTSW 4 115999558 missense probably damaging 1.00
R1465:Faah UTSW 4 115999558 missense probably damaging 1.00
R1861:Faah UTSW 4 116008235 missense probably benign 0.45
R2062:Faah UTSW 4 115998573 missense probably damaging 1.00
R4926:Faah UTSW 4 115999626 intron probably benign
R5162:Faah UTSW 4 116000741 intron probably benign
R5425:Faah UTSW 4 116000796 missense probably null 0.47
R5449:Faah UTSW 4 115999495 splice site probably null
R6236:Faah UTSW 4 115999589 missense probably benign 0.03
R6370:Faah UTSW 4 116003056 missense probably damaging 1.00
R6569:Faah UTSW 4 116017632 missense probably benign
R7384:Faah UTSW 4 116005167 missense probably damaging 1.00
X0024:Faah UTSW 4 116002979 missense possibly damaging 0.77
Predicted Primers PCR Primer

Sequencing Primer
Posted On2014-05-09