Mutagenetix > Incidental Mutation

Incidental Mutation 'R1652:Trcg1'

188793

Institutional Source

Beutler Lab

Gene Symbol

Trcg1

Ensembl Gene

ENSMUSG00000070298

Gene Name

taste receptor cell gene 1

Synonyms

MMRRC Submission

039688-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R1652 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

57236556-57249864 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 57245573 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 551 (D551A)

Ref Sequence

ENSEMBL: ENSMUSP00000091357 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093837]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000093837
AA Change: D551A

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000091357
Gene: ENSMUSG00000070298
AA Change: D551A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
low complexity region	302	335	N/A	INTRINSIC
Blast:SEA	449	549	5e-21	BLAST
low complexity region	580	594	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	G	T	15: 8,201,146	R969L	probably damaging	Het
Adam2	T	A	14: 66,077,251	E37V	probably benign	Het
Adamts7	A	G	9: 90,189,644	D664G	probably damaging	Het
Adamtsl5	A	G	10: 80,342,177	V256A	probably benign	Het
Adrb1	C	T	19: 56,723,273	S301L	possibly damaging	Het
Akap9	A	G	5: 4,077,210	Y3686C	probably damaging	Het
Ap3b2	A	G	7: 81,473,399	S456P	probably damaging	Het
Atp1a4	T	C	1: 172,254,903	Y124C	probably damaging	Het
Bdkrb1	A	T	12: 105,604,243	T23S	probably damaging	Het
Cacna1g	A	G	11: 94,427,404	Y1468H	probably damaging	Het
Cep170b	A	G	12: 112,733,513	D152G	probably damaging	Het
Cers4	T	A	8: 4,516,908		probably null	Het
Cyp2t4	A	T	7: 27,157,390	D285V	possibly damaging	Het
Ddx56	A	T	11: 6,267,679	L14Q	probably damaging	Het
Dennd2d	C	A	3: 106,487,001	R63S	probably benign	Het
Dnah7b	T	A	1: 46,175,390	L1105*	probably null	Het
Eef1e1	A	T	13: 38,656,105	L75I	possibly damaging	Het
Fam76b	A	G	9: 13,835,892	S191G	probably benign	Het
Fat1	T	A	8: 45,025,178	Y2420*	probably null	Het
Fbxw18	T	A	9: 109,690,627	L270F	probably benign	Het
Fech	T	A	18: 64,458,198	H385L	probably benign	Het
Fkbp4	A	T	6: 128,436,674	I2N	probably damaging	Het
Gda	A	T	19: 21,400,678	M339K	probably damaging	Het
Gdpgp1	T	A	7: 80,239,364	M381K	probably benign	Het
Glyctk	C	A	9: 106,157,157	V173L	probably damaging	Het
Gm2056	T	A	12: 88,027,083	V27E	probably benign	Het
Gtf2ird1	G	A	5: 134,395,713	P393L	probably damaging	Het
Kat2a	T	C	11: 100,708,611	N517D	probably damaging	Het
Krt84	G	A	15: 101,525,963	S523F	possibly damaging	Het
Lama1	G	A	17: 67,807,846	R2330Q	probably damaging	Het
Lamc1	C	T	1: 153,249,646	G597E	probably damaging	Het
Lekr1	A	T	3: 65,684,087	S82C	probably benign	Het
Lgi3	T	C	14: 70,531,216	F51S	probably damaging	Het
Lrba	T	C	3: 86,539,938	S2030P	probably damaging	Het
Map4k5	A	G	12: 69,830,427		probably null	Het
Mcoln2	C	A	3: 146,163,635	R32S	possibly damaging	Het
Metap1	A	T	3: 138,462,390	F324L	probably damaging	Het
Moxd2	C	T	6: 40,887,403	R31H	probably damaging	Het
Ncf4	T	A	15: 78,261,034	M274K	possibly damaging	Het
Nup205	T	G	6: 35,238,966	V1747G	probably benign	Het
Olfr290	T	C	7: 84,916,520	V247A	probably damaging	Het
Olfr584	A	G	7: 103,085,806	D91G	probably benign	Het
Olfr958	G	A	9: 39,550,295	T192I	probably benign	Het
Pbx3	C	T	2: 34,224,556	G122D	probably damaging	Het
Plcb3	A	G	19: 6,955,296	F1034L	probably benign	Het
Ppp2r1a	G	A	17: 20,955,974	V153I	probably benign	Het
Prss33	C	G	17: 23,835,141	M30I	probably benign	Het
Prss33	A	T	17: 23,835,142	M30K	probably benign	Het
R3hdm2	G	A	10: 127,495,091	S793N	probably benign	Het
Rab11fip5	C	T	6: 85,348,297	V343M	probably damaging	Het
Rere	A	G	4: 150,612,065		probably benign	Het
Rims1	G	T	1: 22,292,866	P52Q	probably damaging	Het
Scpep1	G	T	11: 88,952,434	S66*	probably null	Het
Setd2	A	G	9: 110,549,864	S632G	probably benign	Het
Shc3	A	T	13: 51,472,839	H129Q	probably damaging	Het
Slc22a20	A	T	19: 5,972,942	M391K	probably damaging	Het
Smurf1	A	T	5: 144,880,664	I712K	probably damaging	Het
Snx25	T	A	8: 46,049,473	I629L	probably damaging	Het
Supt16	A	C	14: 52,177,180	V425G	probably benign	Het
Tnik	G	A	3: 28,604,293	V576I	probably benign	Het
Ubald2	A	G	11: 116,434,352	N15S	probably damaging	Het
Usf1	T	C	1: 171,417,749	I243T	probably damaging	Het
Vmn2r19	A	T	6: 123,315,697	I233F	possibly damaging	Het
Vmn2r63	T	C	7: 42,928,211	N301S	probably benign	Het
Wdr27	A	G	17: 14,917,270	F419L	probably benign	Het

Other mutations in Trcg1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01727:Trcg1	APN	9	57242273	missense	probably benign
IGL01727:Trcg1	APN	9	57242594	missense	probably damaging	0.97
IGL02147:Trcg1	APN	9	57245849	missense	probably benign	0.20
IGL02329:Trcg1	APN	9	57240393	missense	possibly damaging	0.92
IGL02658:Trcg1	APN	9	57242228	nonsense	probably null
IGL02852:Trcg1	APN	9	57241312	missense	possibly damaging	0.94
IGL03163:Trcg1	APN	9	57248347	missense	possibly damaging	0.92
FR4589:Trcg1	UTSW	9	57242202	frame shift	probably null
R0555:Trcg1	UTSW	9	57242333	missense	probably damaging	1.00
R0747:Trcg1	UTSW	9	57241921	missense	probably benign	0.00
R1061:Trcg1	UTSW	9	57245873	missense	possibly damaging	0.66
R1521:Trcg1	UTSW	9	57242465	missense	probably benign	0.36
R1622:Trcg1	UTSW	9	57248672	missense	possibly damaging	0.94
R4677:Trcg1	UTSW	9	57245861	missense	possibly damaging	0.94
R4879:Trcg1	UTSW	9	57246720	missense	probably damaging	0.99
R5013:Trcg1	UTSW	9	57242279	missense	probably damaging	0.99
R5141:Trcg1	UTSW	9	57241304	missense	probably damaging	1.00
R5690:Trcg1	UTSW	9	57241811	missense	probably benign	0.36
R6416:Trcg1	UTSW	9	57241330	missense	possibly damaging	0.46
R6980:Trcg1	UTSW	9	57245573	missense	probably damaging	0.99
R7022:Trcg1	UTSW	9	57241569	missense	possibly damaging	0.46
R7172:Trcg1	UTSW	9	57248335	missense	probably benign	0.01
R7276:Trcg1	UTSW	9	57242579	missense	probably damaging	0.99
R7412:Trcg1	UTSW	9	57241483	missense	probably benign	0.00
R7546:Trcg1	UTSW	9	57248338	missense	probably benign	0.34
R7942:Trcg1	UTSW	9	57242216	missense	probably benign
R8087:Trcg1	UTSW	9	57248674	missense	probably damaging	0.99
R8094:Trcg1	UTSW	9	57242281	missense	probably benign	0.01
R8825:Trcg1	UTSW	9	57241471	missense	probably benign	0.19

Predicted Primers

PCR Primer
(F):5'- ATCCCCATTTACCAAGGAGGCCAG -3'
(R):5'- AAGGTCAGTCATGCTGTACCCTGC -3'

Sequencing Primer
(F):5'- CAGTCATGTACGGAGGTTCTACAC -3'
(R):5'- TGCAGAGACACACACAGCAG -3'

Posted On

2014-05-09