Mutagenetix > Incidental Mutation

Incidental Mutation 'R1653:Atrn'

188835

Institutional Source

Beutler Lab

Gene Symbol

Atrn

Ensembl Gene

ENSMUSG00000027312

Gene Name

attractin

Synonyms

Mgca

MMRRC Submission

039689-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1653 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

130906495-131030333 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 130935624 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 198 (I198V)

Ref Sequence

ENSEMBL: ENSMUSP00000028781 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028781]

AlphaFold

Q9WU60

Predicted Effect

probably benign
Transcript: ENSMUST00000028781
AA Change: I198V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000028781
Gene: ENSMUSG00000027312
AA Change: I198V

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	51	97	N/A	INTRINSIC
EGF	99	129	9.85e-5	SMART
CUB	131	247	7.85e-18	SMART
EGF	248	282	1.47e1	SMART
Pfam:Kelch_1	339	382	1.1e-7	PFAM
Pfam:Kelch_5	389	434	2.5e-7	PFAM
Pfam:Kelch_6	390	439	3.3e-8	PFAM
Pfam:Kelch_1	553	606	8.4e-8	PFAM
PSI	646	693	7.41e-7	SMART
PSI	702	747	8.64e-8	SMART
PSI	754	799	2.11e-2	SMART
CLECT	787	918	6.14e-20	SMART
PSI	931	982	1.11e-5	SMART
PSI	985	1060	1.2e-6	SMART
EGF_Lam	1062	1105	1.97e-4	SMART
EGF_like	1108	1154	3.9e0	SMART
transmembrane domain	1278	1300	N/A	INTRINSIC
low complexity region	1310	1322	N/A	INTRINSIC
low complexity region	1373	1385	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134964

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156982

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.7%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a widely expressed transmembrane glycoprotein that plays important roles in diverse physiological processes such as regulation of hair pigmentation, monocyte-T cell interaction and control of energy homeostasis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Certain mutations in this gene are responsible for the mahogany mouse phenotype of dark brown or black coat on a normally agouti background. Mice with loss-of-function mutations in this gene exhibit black coat color, tremor, adiposity, higher basal metabolic rate, juvenile-onset hypomyelination and age-dependent spongiform neurodegeneration of the central nervous system. [provided by RefSeq, Jul 2016]
PHENOTYPE: Some mutant homozygotes exhibit decreases in phaeomelanin synthesis, body weight, and adiposity; increases in locomotion, and abnormal myelination and vacuolation of the central nervous system resulting in tremors. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130019O22Rik	G	T	7: 127,384,480 (GRCm38)	H483Q	possibly damaging	Het
Adam34	A	T	8: 43,650,645 (GRCm38)	C654*	probably null	Het
Adamts19	T	A	18: 58,890,293 (GRCm38)	N253K	probably benign	Het
Adgrb3	A	G	1: 25,101,503 (GRCm38)	L1162S	probably benign	Het
Ap2b1	T	A	11: 83,346,831 (GRCm38)	Y574N	probably damaging	Het
Bcan	T	A	3: 87,994,196 (GRCm38)	I400F	probably damaging	Het
Capn10	A	G	1: 92,946,898 (GRCm38)	Y617C	probably damaging	Het
Capn8	A	G	1: 182,623,951 (GRCm38)	N578D	probably benign	Het
Casd1	T	C	6: 4,624,134 (GRCm38)	L309P	probably benign	Het
Ccser1	T	A	6: 61,311,465 (GRCm38)	I204K	probably benign	Het
Cd276	T	C	9: 58,537,449 (GRCm38)	T80A	probably benign	Het
Cdh3	T	C	8: 106,539,068 (GRCm38)	S248P	probably damaging	Het
Celsr2	T	C	3: 108,413,520 (GRCm38)	T659A	possibly damaging	Het
Col6a4	C	T	9: 106,072,409 (GRCm38)	V676I	probably damaging	Het
Crmp1	T	A	5: 37,286,468 (GRCm38)	V575D	probably damaging	Het
Ep400	G	A	5: 110,693,174 (GRCm38)	Q1795*	probably null	Het
Gcnt3	A	G	9: 70,035,077 (GRCm38)	C70R	probably damaging	Het
Gm12258	T	C	11: 58,858,287 (GRCm38)	I96T	possibly damaging	Het
Gpr183	A	G	14: 121,954,263 (GRCm38)	F282S	probably damaging	Het
Igfals	T	C	17: 24,881,078 (GRCm38)	V381A	probably benign	Het
Irs3	C	A	5: 137,644,521 (GRCm38)	L218F	probably damaging	Het
Kdm5b	T	C	1: 134,602,481 (GRCm38)	F410S	probably damaging	Het
Klc4	T	C	17: 46,631,859 (GRCm38)	Y593C	possibly damaging	Het
Lce1h	T	A	3: 92,763,443 (GRCm38)	Q134L	unknown	Het
Lyst	T	C	13: 13,635,226 (GRCm38)	S494P	probably damaging	Het
March3	A	G	18: 56,811,895 (GRCm38)	M42T	probably benign	Het
Myh7	A	G	14: 54,990,789 (GRCm38)	I250T	probably benign	Het
N4bp1	G	T	8: 86,844,948 (GRCm38)	H807Q	probably benign	Het
Ndn	C	T	7: 62,348,508 (GRCm38)	P34L	probably benign	Het
Nfs1	C	T	2: 156,125,336 (GRCm38)	G44D	probably damaging	Het
Nrg1	C	T	8: 31,818,653 (GRCm38)	R445H	probably damaging	Het
Olfr1507	A	T	14: 52,490,772 (GRCm38)	F64Y	probably damaging	Het
Olfr299	T	C	7: 86,466,212 (GRCm38)	V267A	probably benign	Het
Olfr683	T	A	7: 105,143,870 (GRCm38)	D141V	possibly damaging	Het
Pak7	C	T	2: 136,116,887 (GRCm38)	V94M	probably damaging	Het
Pdk1	G	A	2: 71,888,995 (GRCm38)		probably null	Het
Sin3b	G	T	8: 72,741,519 (GRCm38)	V290L	probably benign	Het
Sirt1	T	C	10: 63,321,809 (GRCm38)	T609A	probably benign	Het
Skint5	A	T	4: 113,490,678 (GRCm38)	S1289T	unknown	Het
Slc32a1	A	T	2: 158,614,889 (GRCm38)	H488L	probably benign	Het
Slc35b3	A	G	13: 38,955,798 (GRCm38)	S18P	probably benign	Het
Spaca7	T	A	8: 12,586,501 (GRCm38)	I109K	possibly damaging	Het
Tmem204	A	G	17: 25,080,527 (GRCm38)	L6P	possibly damaging	Het
Tubgcp6	G	A	15: 89,107,442 (GRCm38)	R651C	probably damaging	Het
Vps13b	T	A	15: 35,607,272 (GRCm38)	L1117*	probably null	Het
Wdcp	T	C	12: 4,851,815 (GRCm38)	L557P	probably damaging	Het
Wwp2	T	C	8: 107,483,410 (GRCm38)	F140S	possibly damaging	Het
Zfp429	C	T	13: 67,389,924 (GRCm38)	R467H	possibly damaging	Het
Zfp839	T	A	12: 110,855,250 (GRCm38)	M166K	probably benign	Het
Zfyve9	G	A	4: 108,660,577 (GRCm38)	Q1106*	probably null	Het
Zrsr1	T	A	11: 22,974,158 (GRCm38)	C311S	probably damaging	Het

Other mutations in Atrn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00337:Atrn	APN	2	130,958,079 (GRCm38)	missense	probably damaging	1.00
IGL00571:Atrn	APN	2	130,995,048 (GRCm38)	missense	probably damaging	1.00
IGL01092:Atrn	APN	2	130,947,636 (GRCm38)	nonsense	probably null
IGL01572:Atrn	APN	2	131,002,795 (GRCm38)	missense	probably damaging	1.00
IGL01924:Atrn	APN	2	130,935,565 (GRCm38)	missense	probably damaging	1.00
IGL02116:Atrn	APN	2	130,958,089 (GRCm38)	missense	probably damaging	1.00
IGL02372:Atrn	APN	2	131,002,754 (GRCm38)	splice site	probably benign
IGL02390:Atrn	APN	2	131,020,977 (GRCm38)	missense	possibly damaging	0.82
IGL02548:Atrn	APN	2	130,972,282 (GRCm38)	missense	probably damaging	1.00
IGL02749:Atrn	APN	2	130,947,734 (GRCm38)	splice site	probably benign
IGL02749:Atrn	APN	2	130,970,144 (GRCm38)	nonsense	probably null
BB010:Atrn	UTSW	2	130,995,066 (GRCm38)	missense	probably damaging	1.00
BB020:Atrn	UTSW	2	130,995,066 (GRCm38)	missense	probably damaging	1.00
R0026:Atrn	UTSW	2	130,957,920 (GRCm38)	missense	probably damaging	1.00
R0403:Atrn	UTSW	2	130,906,859 (GRCm38)	missense	probably damaging	1.00
R0479:Atrn	UTSW	2	130,999,165 (GRCm38)	nonsense	probably null
R0544:Atrn	UTSW	2	130,986,826 (GRCm38)	missense	probably damaging	1.00
R0570:Atrn	UTSW	2	130,980,134 (GRCm38)	missense	probably benign	0.01
R0606:Atrn	UTSW	2	130,906,856 (GRCm38)	missense	possibly damaging	0.90
R0617:Atrn	UTSW	2	130,995,085 (GRCm38)	critical splice donor site	probably null
R0658:Atrn	UTSW	2	130,970,227 (GRCm38)	critical splice donor site	probably null
R1108:Atrn	UTSW	2	130,957,914 (GRCm38)	missense	probably damaging	1.00
R1112:Atrn	UTSW	2	130,999,161 (GRCm38)	missense	probably benign	0.04
R1219:Atrn	UTSW	2	131,021,007 (GRCm38)	missense	possibly damaging	0.90
R1422:Atrn	UTSW	2	130,957,914 (GRCm38)	missense	probably damaging	1.00
R1524:Atrn	UTSW	2	130,957,080 (GRCm38)	missense	probably benign	0.15
R1795:Atrn	UTSW	2	130,972,288 (GRCm38)	missense	probably benign
R1807:Atrn	UTSW	2	130,982,772 (GRCm38)	missense	possibly damaging	0.94
R1920:Atrn	UTSW	2	130,995,051 (GRCm38)	missense	probably damaging	1.00
R1921:Atrn	UTSW	2	130,995,051 (GRCm38)	missense	probably damaging	1.00
R1935:Atrn	UTSW	2	130,958,035 (GRCm38)	missense	probably damaging	1.00
R1982:Atrn	UTSW	2	130,970,222 (GRCm38)	missense	probably benign
R2000:Atrn	UTSW	2	130,935,588 (GRCm38)	missense	probably damaging	1.00
R2143:Atrn	UTSW	2	130,957,996 (GRCm38)	missense	probably benign	0.03
R2336:Atrn	UTSW	2	130,957,954 (GRCm38)	missense	probably damaging	1.00
R2679:Atrn	UTSW	2	130,961,675 (GRCm38)	critical splice donor site	probably null
R3426:Atrn	UTSW	2	131,020,956 (GRCm38)	missense	probably benign	0.06
R3909:Atrn	UTSW	2	130,994,207 (GRCm38)	missense	probably damaging	1.00
R4077:Atrn	UTSW	2	130,964,930 (GRCm38)	critical splice donor site	probably null
R4162:Atrn	UTSW	2	130,994,228 (GRCm38)	splice site	probably benign
R4195:Atrn	UTSW	2	130,933,412 (GRCm38)	missense	probably damaging	1.00
R4364:Atrn	UTSW	2	130,970,208 (GRCm38)	missense	probably benign	0.39
R4465:Atrn	UTSW	2	130,960,468 (GRCm38)	missense	probably benign	0.08
R4510:Atrn	UTSW	2	130,935,577 (GRCm38)	nonsense	probably null
R4511:Atrn	UTSW	2	130,935,577 (GRCm38)	nonsense	probably null
R4527:Atrn	UTSW	2	130,973,504 (GRCm38)	missense	probably benign	0.10
R4586:Atrn	UTSW	2	130,982,042 (GRCm38)	missense	probably damaging	1.00
R4592:Atrn	UTSW	2	130,999,130 (GRCm38)	intron	probably benign
R4658:Atrn	UTSW	2	130,933,429 (GRCm38)	missense	probably damaging	1.00
R4735:Atrn	UTSW	2	131,020,990 (GRCm38)	missense	probably benign	0.06
R4960:Atrn	UTSW	2	130,995,047 (GRCm38)	nonsense	probably null
R4999:Atrn	UTSW	2	130,975,954 (GRCm38)	missense	probably damaging	1.00
R5066:Atrn	UTSW	2	130,994,193 (GRCm38)	missense	possibly damaging	0.60
R5080:Atrn	UTSW	2	130,970,124 (GRCm38)	missense	possibly damaging	0.95
R5141:Atrn	UTSW	2	130,999,130 (GRCm38)	intron	probably benign
R5256:Atrn	UTSW	2	130,946,019 (GRCm38)	missense	probably benign	0.39
R5494:Atrn	UTSW	2	131,023,075 (GRCm38)	missense	probably damaging	1.00
R5678:Atrn	UTSW	2	130,970,016 (GRCm38)	missense	probably damaging	0.96
R5752:Atrn	UTSW	2	130,906,544 (GRCm38)	unclassified	probably benign
R5931:Atrn	UTSW	2	130,933,436 (GRCm38)	missense	possibly damaging	0.56
R6023:Atrn	UTSW	2	131,020,980 (GRCm38)	missense	probably benign	0.25
R6176:Atrn	UTSW	2	130,946,091 (GRCm38)	missense	probably benign	0.31
R6377:Atrn	UTSW	2	130,979,969 (GRCm38)	missense	probably damaging	1.00
R6433:Atrn	UTSW	2	131,023,027 (GRCm38)	missense	probably damaging	1.00
R7226:Atrn	UTSW	2	130,986,744 (GRCm38)	missense	probably damaging	0.99
R7402:Atrn	UTSW	2	130,947,600 (GRCm38)	missense	probably damaging	1.00
R7541:Atrn	UTSW	2	130,961,571 (GRCm38)	missense	possibly damaging	0.46
R7587:Atrn	UTSW	2	130,980,114 (GRCm38)	missense	probably damaging	1.00
R7872:Atrn	UTSW	2	130,970,227 (GRCm38)	critical splice donor site	probably null
R7910:Atrn	UTSW	2	130,964,887 (GRCm38)	missense	probably benign	0.04
R7913:Atrn	UTSW	2	130,970,211 (GRCm38)	missense	probably damaging	1.00
R7933:Atrn	UTSW	2	130,995,066 (GRCm38)	missense	probably damaging	1.00
R8044:Atrn	UTSW	2	130,935,529 (GRCm38)	missense	probably damaging	1.00
R8079:Atrn	UTSW	2	131,013,641 (GRCm38)	missense	probably null	1.00
R8093:Atrn	UTSW	2	130,975,988 (GRCm38)	missense	probably benign	0.00
R8203:Atrn	UTSW	2	130,960,549 (GRCm38)	missense	probably benign	0.00
R8234:Atrn	UTSW	2	131,023,000 (GRCm38)	critical splice acceptor site	probably null
R8462:Atrn	UTSW	2	130,935,584 (GRCm38)	missense	probably damaging	1.00
R8816:Atrn	UTSW	2	131,004,574 (GRCm38)	missense	probably damaging	1.00
R8816:Atrn	UTSW	2	130,906,878 (GRCm38)	missense	probably damaging	1.00
R8831:Atrn	UTSW	2	130,906,601 (GRCm38)	missense	probably benign	0.22
R8937:Atrn	UTSW	2	130,999,237 (GRCm38)	missense	probably benign	0.00
R9161:Atrn	UTSW	2	130,935,550 (GRCm38)	missense	probably damaging	1.00
R9722:Atrn	UTSW	2	130,961,616 (GRCm38)	missense	probably damaging	1.00
R9786:Atrn	UTSW	2	130,944,889 (GRCm38)	missense	probably damaging	1.00
RF009:Atrn	UTSW	2	130,906,922 (GRCm38)	missense	probably benign	0.12
X0024:Atrn	UTSW	2	130,958,139 (GRCm38)	missense	probably damaging	1.00
Z1088:Atrn	UTSW	2	130,973,399 (GRCm38)	missense	probably benign
Z1176:Atrn	UTSW	2	130,946,193 (GRCm38)	missense	probably benign	0.27
Z1177:Atrn	UTSW	2	130,946,042 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGAAGTCAGAGCCCGTGTCTCTTTT -3'
(R):5'- ACCAAGTGAACTGTAGCTCACCAGA -3'

Sequencing Primer
(F):5'- TCTCTGACAAATGCAGGAAAGC -3'
(R):5'- CAGAACAGAACTAATTCCATGAGATG -3'

Posted On

2014-05-09