Mutagenetix > Incidental Mutation

Incidental Mutation 'R1655:Zfp609'

189037

Institutional Source

Beutler Lab

Gene Symbol

Zfp609

Ensembl Gene

ENSMUSG00000040524

Gene Name

zinc finger protein 609

Synonyms

MMRRC Submission

039691-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.445) question?

Stock #

R1655 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

65692391-65827564 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 65703554 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 709 (V709A)

Ref Sequence

ENSEMBL: ENSMUSP00000124089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159109]

AlphaFold

Q8BZ47

Predicted Effect

possibly damaging
Transcript: ENSMUST00000159109
AA Change: V709A

PolyPhen 2 Score 0.455 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000124089
Gene: ENSMUSG00000040524
AA Change: V709A

Domain	Start	End	E-Value	Type
low complexity region	83	101	N/A	INTRINSIC
low complexity region	125	139	N/A	INTRINSIC
low complexity region	155	176	N/A	INTRINSIC
low complexity region	259	269	N/A	INTRINSIC
low complexity region	365	376	N/A	INTRINSIC
low complexity region	386	405	N/A	INTRINSIC
ZnF_C2H2	495	520	2.14e0	SMART
low complexity region	629	638	N/A	INTRINSIC
low complexity region	657	667	N/A	INTRINSIC
low complexity region	724	738	N/A	INTRINSIC
low complexity region	1000	1020	N/A	INTRINSIC
low complexity region	1210	1219	N/A	INTRINSIC
low complexity region	1257	1273	N/A	INTRINSIC
low complexity region	1329	1348	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159878

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.0%
20x: 91.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833427G06Rik	A	T	9: 51,083,621	I136N	probably damaging	Het
9530053A07Rik	A	T	7: 28,147,110	N1076Y	probably damaging	Het
A730061H03Rik	A	T	14: 55,560,333		probably benign	Het
Abca1	C	T	4: 53,050,964	A1582T	probably benign	Het
Acot8	A	T	2: 164,803,108	S52T	probably benign	Het
Atcay	C	T	10: 81,213,397	V124M	probably damaging	Het
Cep295	C	T	9: 15,340,883	E397K	probably damaging	Het
Cfap46	A	T	7: 139,642,520	Y1180*	probably null	Het
Clptm1	T	A	7: 19,645,867	H148L	probably benign	Het
Clstn3	A	G	6: 124,437,427	L743P	probably damaging	Het
Crtc3	A	T	7: 80,598,776	M313K	possibly damaging	Het
Csgalnact1	T	A	8: 68,373,689	I326F	possibly damaging	Het
Dennd6b	G	T	15: 89,196,340	T19K	unknown	Het
Disp1	A	T	1: 183,087,004	I1284N	probably benign	Het
Dnah2	A	G	11: 69,473,854	Y1992H	probably damaging	Het
Dnah6	C	T	6: 73,205,732	V205I	possibly damaging	Het
Dst	G	T	1: 34,282,576	G4391*	probably null	Het
Dytn	A	G	1: 63,661,198	S258P	probably damaging	Het
Emilin3	T	A	2: 160,910,866		probably null	Het
Ermn	C	T	2: 58,052,584	V45I	probably benign	Het
Fat4	T	C	3: 38,957,318	V2189A	probably damaging	Het
Filip1l	T	C	16: 57,571,851	I934T	probably damaging	Het
Gbp9	T	A	5: 105,081,692	Q472L	possibly damaging	Het
Gimap5	G	T	6: 48,753,176	E227*	probably null	Het
Gsdmc	C	T	15: 63,780,043	V240M	probably benign	Het
H2-Q4	G	T	17: 35,382,905	V248F	probably damaging	Het
Helz2	T	C	2: 181,234,147	E1518G	probably damaging	Het
Hmcn1	A	G	1: 150,630,333	V3814A	probably benign	Het
Ifna7	A	G	4: 88,816,660	T145A	probably benign	Het
Itgam	T	A	7: 128,115,163	M947K	probably benign	Het
Itpr2	T	G	6: 146,376,148	N608H	probably damaging	Het
Klra2	T	A	6: 131,220,211	N242I	probably damaging	Het
Lonrf2	A	T	1: 38,811,824	L219Q	probably damaging	Het
Ly6c2	T	C	15: 75,108,563	I126V	probably benign	Het
Mr1	G	A	1: 155,132,455	T258M	probably benign	Het
Mrps35	T	G	6: 147,060,228	D200E	possibly damaging	Het
Nbeal2	A	C	9: 110,632,872	S1506A	probably damaging	Het
Ncoa7	T	C	10: 30,698,245		probably null	Het
Nlrp4a	A	T	7: 26,449,651	I228F	possibly damaging	Het
Olfr1047	A	G	2: 86,228,080	V297A	possibly damaging	Het
Olfr1339	A	G	4: 118,734,999	S157G	probably benign	Het
Olfr368	A	G	2: 37,331,939	Y64C	probably damaging	Het
Olfr483	A	T	7: 108,103,464	I52F	probably damaging	Het
Paxx	T	C	2: 25,460,316	E93G	probably damaging	Het
Per2	C	A	1: 91,448,768	G128W	probably damaging	Het
Piezo1	A	G	8: 122,496,822	I796T	probably benign	Het
Pkhd1	A	G	1: 20,584,129	S235P	probably damaging	Het
Pole	T	A	5: 110,335,922	F259Y	probably damaging	Het
Pus7	T	A	5: 23,747,800	K512*	probably null	Het
Ralyl	A	T	3: 14,107,236	Y55F	probably damaging	Het
Rgs14	T	A	13: 55,383,534	M451K	probably benign	Het
Rhag	T	C	17: 40,831,596	F231L	probably damaging	Het
Ric8a	T	C	7: 140,860,895	C94R	probably benign	Het
Rictor	T	A	15: 6,772,212	D460E	probably benign	Het
Rpn1	T	C	6: 88,100,944	V454A	possibly damaging	Het
Sacs	A	G	14: 61,191,782	D427G	probably benign	Het
Scai	A	T	2: 39,080,117	V545D	possibly damaging	Het
Serpinb3a	A	G	1: 107,046,212	V323A	probably damaging	Het
Slc13a5	C	A	11: 72,257,378	C277F	probably benign	Het
Slc15a1	A	T	14: 121,465,899	Y557N	probably benign	Het
Slc34a2	T	C	5: 53,069,419	V628A	probably benign	Het
Slc8a2	G	T	7: 16,141,135	G436V	probably damaging	Het
Sphkap	G	A	1: 83,277,515	R838*	probably null	Het
Supt5	T	C	7: 28,330,024	I103V	probably benign	Het
Tdrd1	T	A	19: 56,843,216	Y346*	probably null	Het
Tg	T	G	15: 66,828,568		probably null	Het
Top1	T	A	2: 160,703,696		probably null	Het
Trmt12	T	C	15: 58,873,227	L158P	probably damaging	Het
Tssk4	A	G	14: 55,651,695	N226S	probably damaging	Het
Unc80	G	T	1: 66,672,756	V2746F	possibly damaging	Het
Usp34	T	A	11: 23,375,051	V999E	probably benign	Het
Virma	T	C	4: 11,494,786	V29A	probably damaging	Het
Zfp40	A	T	17: 23,177,266	Y48N	probably benign	Het

Other mutations in Zfp609

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00956:Zfp609	APN	9	65702763	missense	probably benign	0.18
IGL01688:Zfp609	APN	9	65704025	missense	probably benign
IGL01718:Zfp609	APN	9	65702400	nonsense	probably null
IGL01860:Zfp609	APN	9	65702834	missense	possibly damaging	0.82
IGL02441:Zfp609	APN	9	65703329	missense	possibly damaging	0.92
IGL02490:Zfp609	APN	9	65703968	missense	possibly damaging	0.92
IGL02614:Zfp609	APN	9	65702790	missense	probably damaging	0.96
IGL02794:Zfp609	APN	9	65704320	missense	possibly damaging	0.83
IGL02959:Zfp609	APN	9	65703393	missense	probably benign
IGL02967:Zfp609	APN	9	65697619	missense	possibly damaging	0.83
IGL03036:Zfp609	APN	9	65702645	missense	possibly damaging	0.89
IGL03182:Zfp609	APN	9	65701005	missense	probably benign	0.02
H8441:Zfp609	UTSW	9	65794887	missense	possibly damaging	0.82
R0304:Zfp609	UTSW	9	65701188	missense	possibly damaging	0.89
R0487:Zfp609	UTSW	9	65702634	missense	unknown
R0505:Zfp609	UTSW	9	65703462	missense	possibly damaging	0.92
R0684:Zfp609	UTSW	9	65731201	missense	probably benign	0.01
R1480:Zfp609	UTSW	9	65703311	missense	possibly damaging	0.82
R1507:Zfp609	UTSW	9	65794777	missense	possibly damaging	0.92
R1579:Zfp609	UTSW	9	65704472	missense	possibly damaging	0.90
R1692:Zfp609	UTSW	9	65795311	missense	probably damaging	0.98
R1701:Zfp609	UTSW	9	65731000	missense	probably benign	0.05
R1735:Zfp609	UTSW	9	65703092	nonsense	probably null
R1883:Zfp609	UTSW	9	65794758	missense	probably benign
R1970:Zfp609	UTSW	9	65795277	missense	probably damaging	0.96
R2059:Zfp609	UTSW	9	65704434	missense	possibly damaging	0.83
R2302:Zfp609	UTSW	9	65794897	missense	possibly damaging	0.66
R3404:Zfp609	UTSW	9	65701172	missense	possibly damaging	0.68
R3405:Zfp609	UTSW	9	65701172	missense	possibly damaging	0.68
R4514:Zfp609	UTSW	9	65703695	missense	possibly damaging	0.46
R4533:Zfp609	UTSW	9	65703608	missense	probably benign	0.00
R5043:Zfp609	UTSW	9	65700827	missense	probably damaging	0.97
R5499:Zfp609	UTSW	9	65702855	missense	probably benign	0.00
R7081:Zfp609	UTSW	9	65702441	missense	possibly damaging	0.46
R7514:Zfp609	UTSW	9	65706136	missense	probably benign	0.05
R7677:Zfp609	UTSW	9	65697174	missense	possibly damaging	0.61
R7684:Zfp609	UTSW	9	65731080	missense	possibly damaging	0.66
R8229:Zfp609	UTSW	9	65703500	missense	possibly damaging	0.66
R8266:Zfp609	UTSW	9	65703714	missense	possibly damaging	0.66
R8278:Zfp609	UTSW	9	65697522	missense	possibly damaging	0.90
R8934:Zfp609	UTSW	9	65703279	missense	possibly damaging	0.90
R8938:Zfp609	UTSW	9	65703279	missense	possibly damaging	0.90
R8940:Zfp609	UTSW	9	65703279	missense	possibly damaging	0.90
R8986:Zfp609	UTSW	9	65703279	missense	possibly damaging	0.90
R9267:Zfp609	UTSW	9	65701046	missense	possibly damaging	0.90
R9456:Zfp609	UTSW	9	65703843	missense
R9561:Zfp609	UTSW	9	65697230	nonsense	probably null
X0028:Zfp609	UTSW	9	65697080	small deletion	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCCCAGCATCAGAGATGTCAGAG -3'
(R):5'- GCTTAAAATCAGCCCGCCCCATTG -3'

Sequencing Primer
(F):5'- ATACTAGCTAACCGGCTTTGG -3'
(R):5'- CCATTGCCCCTGCCATC -3'

Posted On

2014-05-09