Mutagenetix > Incidental Mutation

Incidental Mutation 'R1700:Hnrnpll'

189694

Institutional Source

Beutler Lab

Gene Symbol

Hnrnpll

Ensembl Gene

ENSMUSG00000024095

Gene Name

heterogeneous nuclear ribonucleoprotein L-like

Synonyms

2510028H02Rik, Hnrpll, 2810036L13Rik

MMRRC Submission

039733-MU

Accession Numbers

Genbank: NM_144802; MGI: 1919942

Essential gene?

Probably essential (E-score: 0.771) question?

Stock #

R1700 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

80029487-80062334 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 80034105 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Alanine at position 502 (S502A)

Ref Sequence

ENSEMBL: ENSMUSP00000139372 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061331] [ENSMUST00000184297] [ENSMUST00000184635]

AlphaFold

Q921F4

Predicted Effect

probably benign
Transcript: ENSMUST00000061331
AA Change: S502A

PolyPhen 2 Score 0.184 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000058308
Gene: ENSMUSG00000024095
AA Change: S502A

Domain	Start	End	E-Value	Type
low complexity region	52	104	N/A	INTRINSIC
RRM	126	195	2.99e-4	SMART
RRM	216	289	1.26e-2	SMART
low complexity region	314	325	N/A	INTRINSIC
RRM	385	454	1.36e-7	SMART
Blast:RRM_2	504	582	3e-32	BLAST

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000157558

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000183516

Predicted Effect

probably benign
Transcript: ENSMUST00000184297

SMART Domains

Protein: ENSMUSP00000139075
Gene: ENSMUSG00000024095

Domain	Start	End	E-Value	Type
low complexity region	52	104	N/A	INTRINSIC
RRM	126	195	2.99e-4	SMART
RRM	216	289	1.26e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184578

Predicted Effect

probably benign
Transcript: ENSMUST00000184635
AA Change: S502A

PolyPhen 2 Score 0.184 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000139372
Gene: ENSMUSG00000024095
AA Change: S502A

Domain	Start	End	E-Value	Type
low complexity region	52	104	N/A	INTRINSIC
RRM	126	195	2.99e-4	SMART
RRM	216	289	1.26e-2	SMART
low complexity region	314	325	N/A	INTRINSIC
RRM	385	454	1.36e-7	SMART
Blast:RRM_2	504	582	3e-32	BLAST

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] HNRNPLL is a master regulator of activation-induced alternative splicing in T cells. In particular, it alters splicing of CD45 (PTPRC; MIM 151460), a tyrosine phosphatase essential for T-cell development and activation (Oberdoerffer et al., 2008 [PubMed 18669861]).[supplied by OMIM, Aug 2008]
PHENOTYPE: Mice homozygous for a point mutation in a RNA recognition motif of the gene product have defects in the generation of alternative transcripts normally found in memory T cells. Total CD4⁺ T cell counts are lower, with a reduction of naï¿½ve CD44^lo T cells occurring as mice age. [provided by MGI curators]

Allele List at MGI

All alleles(6) : Gene trapped(5) Chemically induced(1)

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402H24Rik	G	A	2: 130,709,938 (GRCm38)	L1010F	probably damaging	Het
Abcb1b	T	C	5: 8,849,537 (GRCm38)	F936L	probably benign	Het
Adamts12	T	A	15: 11,152,057 (GRCm38)	I211N	probably benign	Het
Adamts16	G	A	13: 70,779,518 (GRCm38)		probably benign	Het
Alas1	C	T	9: 106,239,646 (GRCm38)	V293I	possibly damaging	Het
Ap1g1	T	C	8: 109,853,612 (GRCm38)	Y569H	probably damaging	Het
Apc2	A	G	10: 80,312,769 (GRCm38)	D1190G	probably damaging	Het
Astn2	G	A	4: 65,746,354 (GRCm38)	Q679*	probably null	Het
Atp2a1	T	A	7: 126,462,909 (GRCm38)	H5L	probably damaging	Het
Baiap3	T	C	17: 25,249,328 (GRCm38)	K279E	probably damaging	Het
C2cd2l	G	A	9: 44,316,612 (GRCm38)	P111S	probably benign	Het
C7	T	A	15: 5,002,792 (GRCm38)	K646*	probably null	Het
Ccp110	C	T	7: 118,735,313 (GRCm38)	T1003I	probably damaging	Het
Cdh22	T	C	2: 165,170,796 (GRCm38)	D123G	probably damaging	Het
Cep295	C	T	9: 15,340,883 (GRCm38)	E397K	probably damaging	Het
Clip1	A	G	5: 123,630,370 (GRCm38)	V722A	probably benign	Het
Cpsf4l	A	G	11: 113,702,075 (GRCm38)	F174S	probably benign	Het
Ctnna3	T	C	10: 63,852,772 (GRCm38)	C332R	probably damaging	Het
Cxcl10	A	T	5: 92,347,855 (GRCm38)	N76K	probably damaging	Het
Dnaja3	G	A	16: 4,684,165 (GRCm38)	R11K	probably null	Het
Efcab10	A	G	12: 33,395,171 (GRCm38)	T28A	possibly damaging	Het
Esrrg	A	G	1: 188,043,653 (GRCm38)	R103G	probably damaging	Het
Exosc2	A	G	2: 31,670,806 (GRCm38)	K23E	probably benign	Het
Flot2	C	T	11: 78,049,547 (GRCm38)	S40L	possibly damaging	Het
Fsip2	T	C	2: 82,991,737 (GRCm38)	V5938A	probably benign	Het
Gclc	A	G	9: 77,776,289 (GRCm38)	T143A	probably benign	Het
Gpr37	A	G	6: 25,669,624 (GRCm38)	V407A	probably benign	Het
Gucy2e	A	T	11: 69,232,058 (GRCm38)	M497K	probably benign	Het
Herc1	T	A	9: 66,450,678 (GRCm38)		probably null	Het
Ipo11	G	T	13: 106,795,662 (GRCm38)	T975N	probably benign	Het
Kif17	C	T	4: 138,262,698 (GRCm38)	Q66*	probably null	Het
Lrp10	T	C	14: 54,469,752 (GRCm38)	V682A	possibly damaging	Het
Lsmem1	A	T	12: 40,180,678 (GRCm38)	L75Q	probably damaging	Het
Med16	A	T	10: 79,899,335 (GRCm38)	Y430N	probably benign	Het
Med8	C	T	4: 118,412,734 (GRCm38)	S72L	possibly damaging	Het
Mex3a	A	G	3: 88,536,375 (GRCm38)	T253A	probably damaging	Het
Myf6	T	C	10: 107,493,359 (GRCm38)	K242E	probably damaging	Het
Ncald	T	A	15: 37,397,343 (GRCm38)	Y31F	probably benign	Het
Ndufa12	T	A	10: 94,199,993 (GRCm38)	Y48N	probably damaging	Het
Olfr1111	T	G	2: 87,149,779 (GRCm38)	N294T	probably damaging	Het
Olfr1145	T	C	2: 87,810,768 (GRCm38)	V316A	probably benign	Het
Olfr638	T	A	7: 104,004,122 (GRCm38)	Y282*	probably null	Het
Pde4dip	T	C	3: 97,703,323 (GRCm38)	T1858A	probably benign	Het
Pgbd1	A	G	13: 21,434,481 (GRCm38)	L2P	probably damaging	Het
Piezo1	C	T	8: 122,487,502 (GRCm38)	R1642H	probably damaging	Het
Plxna1	A	T	6: 89,357,008 (GRCm38)	M213K	probably damaging	Het
Polr1b	A	G	2: 129,123,121 (GRCm38)	N709S	probably damaging	Het
Pramel1	T	A	4: 143,398,429 (GRCm38)	W308R	probably damaging	Het
Prkag2	A	T	5: 24,871,541 (GRCm38)	I208N	probably damaging	Het
Ptpro	T	A	6: 137,443,594 (GRCm38)	V1007D	probably damaging	Het
Rngtt	T	A	4: 33,330,864 (GRCm38)	F156I	probably damaging	Het
Rpusd3	A	G	6: 113,415,533 (GRCm38)	*345Q	probably null	Het
Rrp1b	A	G	17: 32,057,204 (GRCm38)	K575R	probably benign	Het
Shd	G	A	17: 55,974,307 (GRCm38)	V250I	probably damaging	Het
Slc12a5	C	A	2: 164,992,376 (GRCm38)	N749K	possibly damaging	Het
Sorbs2	T	A	8: 45,800,984 (GRCm38)	V488D	probably damaging	Het
Sphkap	G	A	1: 83,277,515 (GRCm38)	R838*	probably null	Het
Srsf4	C	A	4: 131,900,560 (GRCm38)		probably benign	Het
Strn	T	C	17: 78,692,402 (GRCm38)	Y135C	probably damaging	Het
Synm	T	C	7: 67,759,628 (GRCm38)	M1V	probably null	Het
Tas1r3	T	G	4: 155,861,570 (GRCm38)	Q489P	probably benign	Het
Tas2r103	A	T	6: 133,036,811 (GRCm38)	N97K	probably damaging	Het
Tas2r113	A	G	6: 132,893,792 (GRCm38)	Y261C	possibly damaging	Het
Tex21	T	C	12: 76,221,672 (GRCm38)	E112G	probably damaging	Het
Trappc8	T	C	18: 20,832,998 (GRCm38)	S1128G	probably damaging	Het
Ubap1l	A	G	9: 65,371,743 (GRCm38)		probably benign	Het
Ubash3a	A	G	17: 31,215,044 (GRCm38)	D121G	probably damaging	Het
Ubxn4	A	G	1: 128,252,286 (GRCm38)	I56V	possibly damaging	Het
Uri1	T	C	7: 37,963,524 (GRCm38)	I348M	probably damaging	Het
Usp21	C	A	1: 171,283,722 (GRCm38)	L379F	probably damaging	Het
Vmn1r159	T	A	7: 22,842,965 (GRCm38)	H214L	probably damaging	Het
Zdbf2	A	G	1: 63,302,741 (GRCm38)	E93G	unknown	Het

Other mutations in Hnrnpll

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
thunder	APN	17	80,053,571 (GRCm38)	missense	probably damaging	1.00
IGL01989:Hnrnpll	APN	17	80,038,740 (GRCm38)	missense	probably benign	0.15
IGL02093:Hnrnpll	APN	17	80,044,504 (GRCm38)	missense	probably benign	0.00
IGL02141:Hnrnpll	APN	17	80,050,713 (GRCm38)	missense	probably benign	0.02
IGL02749:Hnrnpll	APN	17	80,061,991 (GRCm38)	start codon destroyed	probably null
IGL03213:Hnrnpll	APN	17	80,034,098 (GRCm38)	missense	probably damaging	1.00
Grell	UTSW	17	80,034,105 (GRCm38)	missense	probably damaging	1.00
Lindsley	UTSW	17	80,049,847 (GRCm38)	missense	probably damaging	1.00
R0477:Hnrnpll	UTSW	17	80,061,832 (GRCm38)	missense	unknown
R1599:Hnrnpll	UTSW	17	80,053,625 (GRCm38)	missense	unknown
R1838:Hnrnpll	UTSW	17	80,038,623 (GRCm38)	missense	probably damaging	1.00
R1907:Hnrnpll	UTSW	17	80,035,329 (GRCm38)	critical splice donor site	probably null
R1978:Hnrnpll	UTSW	17	80,044,518 (GRCm38)	missense	probably benign	0.01
R2079:Hnrnpll	UTSW	17	80,035,377 (GRCm38)	missense	probably benign	0.01
R4061:Hnrnpll	UTSW	17	80,032,772 (GRCm38)	missense	probably benign	0.01
R4062:Hnrnpll	UTSW	17	80,032,772 (GRCm38)	missense	probably benign	0.01
R4064:Hnrnpll	UTSW	17	80,032,772 (GRCm38)	missense	probably benign	0.01
R4226:Hnrnpll	UTSW	17	80,049,805 (GRCm38)	critical splice donor site	probably null
R4625:Hnrnpll	UTSW	17	80,050,862 (GRCm38)	nonsense	probably null
R5175:Hnrnpll	UTSW	17	80,034,070 (GRCm38)	missense	possibly damaging	0.83
R5232:Hnrnpll	UTSW	17	80,038,678 (GRCm38)	missense	probably damaging	1.00
R5620:Hnrnpll	UTSW	17	80,038,622 (GRCm38)	missense	probably damaging	1.00
R5978:Hnrnpll	UTSW	17	80,034,191 (GRCm38)	missense	probably damaging	1.00
R6183:Hnrnpll	UTSW	17	80,049,876 (GRCm38)	missense	possibly damaging	0.46
R6374:Hnrnpll	UTSW	17	80,049,874 (GRCm38)	missense	possibly damaging	0.51
R7120:Hnrnpll	UTSW	17	80,034,057 (GRCm38)	missense	probably benign	0.01
R7429:Hnrnpll	UTSW	17	80,049,847 (GRCm38)	missense	probably damaging	1.00
R7430:Hnrnpll	UTSW	17	80,049,847 (GRCm38)	missense	probably damaging	1.00
R7576:Hnrnpll	UTSW	17	80,044,514 (GRCm38)	missense	possibly damaging	0.91
R8001:Hnrnpll	UTSW	17	80,038,723 (GRCm38)	nonsense	probably null
R8010:Hnrnpll	UTSW	17	80,061,956 (GRCm38)	missense	unknown
R8060:Hnrnpll	UTSW	17	80,034,105 (GRCm38)	missense	probably damaging	1.00
R8068:Hnrnpll	UTSW	17	80,050,852 (GRCm38)	missense	possibly damaging	0.80
R8381:Hnrnpll	UTSW	17	80,030,491 (GRCm38)	missense	probably damaging	1.00
R9378:Hnrnpll	UTSW	17	80,061,862 (GRCm38)	missense	unknown
R9488:Hnrnpll	UTSW	17	80,061,956 (GRCm38)	missense	unknown
Z1177:Hnrnpll	UTSW	17	80,048,610 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGTAGGCTCTACGTACACAAACCCA -3'
(R):5'- TCCTGGACCAATCCCCTTGGATAAA -3'

Sequencing Primer
(F):5'- ACCAAACTAACAACAAAAAGCAGG -3'
(R):5'- GATAAATGGTTGCCTGCACC -3'

Posted On

2014-05-14