Incidental Mutation 'R0020:Grasp'
Institutional Source Beutler Lab
Gene Symbol Grasp
Ensembl Gene ENSMUSG00000000531
Gene NameGRP1 (general receptor for phosphoinositides 1)-associated scaffold protein
MMRRC Submission 038315-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.156) question?
Stock #R0020 (G1)
Quality Score
Status Validated
Chromosomal Location101224188-101232756 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 101230552 bp
Amino Acid Change Valine to Alanine at position 157 (V157A)
Ref Sequence ENSEMBL: ENSMUSP00000000543 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000000543]
Predicted Effect probably damaging
Transcript: ENSMUST00000000543
AA Change: V157A

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000000543
Gene: ENSMUSG00000000531
AA Change: V157A

low complexity region 30 57 N/A INTRINSIC
PDZ 109 189 2.12e-13 SMART
low complexity region 248 277 N/A INTRINSIC
low complexity region 291 312 N/A INTRINSIC
low complexity region 336 347 N/A INTRINSIC
Meta Mutation Damage Score 0.6200 question?
Coding Region Coverage
  • 1x: 82.6%
  • 3x: 76.6%
  • 10x: 60.3%
  • 20x: 42.3%
Validation Efficiency 92% (100/109)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that functions as a molecular scaffold, linking receptors, including group 1 metabotropic glutamate receptors, to neuronal proteins. The encoded protein contains conserved domains, including a leucine zipper sequence, PDZ domain and a C-terminal PDZ-binding motif. Alternately spliced transcript variants have been observed for this gene.[provided by RefSeq, Dec 2012]
PHENOTYPE: Mice homozygous for targeted null mutations develop and behave normally under ordinary conditions but display a marked reduction in sensitivity to acute morphine responses and impaired adaptive responses to morphine and cocaine. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 39 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9930021J03Rik A T 19: 29,716,197 D2032E probably damaging Het
Agfg2 C T 5: 137,653,802 V432M probably benign Het
Akap11 A T 14: 78,518,177 I74K probably benign Het
Atf2 T C 2: 73,846,284 D122G possibly damaging Het
AW549877 T C 15: 3,991,868 probably benign Het
C330027C09Rik A T 16: 49,001,612 H201L probably damaging Het
Ccser1 C A 6: 61,313,804 T490K possibly damaging Het
Clstn1 G A 4: 149,634,796 V361M probably damaging Het
Cnga4 G T 7: 105,405,677 R53L probably damaging Het
Col6a3 T A 1: 90,811,550 I319F probably damaging Het
Cst11 T A 2: 148,771,333 Y24F probably damaging Het
Cstb T A 10: 78,427,336 V65E probably benign Het
Cyp2j11 G A 4: 96,307,404 H352Y probably benign Het
Ezr G T 17: 6,742,727 Q308K probably damaging Het
F3 A T 3: 121,731,616 N169Y probably damaging Het
Fbp2 A T 13: 62,854,048 F118I probably damaging Het
Fcho1 C T 8: 71,716,870 G131R probably benign Het
Fhl5 A T 4: 25,200,054 V260E probably benign Het
Gm6614 A T 6: 141,972,350 V600E possibly damaging Het
Kcna10 A T 3: 107,195,420 I456F probably damaging Het
Loxl2 T C 14: 69,660,793 V232A probably damaging Het
Megf10 G T 18: 57,287,893 V868F possibly damaging Het
Megf9 A G 4: 70,488,149 V260A probably benign Het
Nampt A T 12: 32,841,013 S278C probably damaging Het
Nap1l1 A C 10: 111,491,023 E148D probably benign Het
Pamr1 C T 2: 102,642,078 T574I probably benign Het
Pde4d T A 13: 109,954,570 C35S possibly damaging Het
Pkd1l1 A G 11: 8,875,765 probably benign Het
Pkd2 A G 5: 104,503,516 E910G probably damaging Het
Pkhd1l1 A G 15: 44,556,872 Y3002C probably damaging Het
Ppp6r2 T A 15: 89,259,139 M163K probably damaging Het
Prdm4 T C 10: 85,907,623 N256S probably benign Het
Ptpro T A 6: 137,443,594 V1007D probably damaging Het
Scube2 A G 7: 109,830,888 probably benign Het
Sptbn5 T A 2: 120,065,631 I779F probably damaging Het
Uspl1 A G 5: 149,209,779 T447A probably damaging Het
Zfp282 T G 6: 47,880,009 W59G probably damaging Het
Zfp629 C G 7: 127,611,169 E489D probably benign Het
Zfp746 C A 6: 48,064,707 A362S probably benign Het
Other mutations in Grasp
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01062:Grasp APN 15 101228896 splice site probably benign
IGL02069:Grasp APN 15 101224465 missense probably damaging 1.00
IGL02516:Grasp APN 15 101229051 missense probably damaging 1.00
IGL02997:Grasp APN 15 101231018 missense probably damaging 1.00
IGL03079:Grasp APN 15 101230567 missense probably damaging 1.00
R0020:Grasp UTSW 15 101230552 missense probably damaging 1.00
R1916:Grasp UTSW 15 101226969 splice site probably benign
R1952:Grasp UTSW 15 101224500 missense probably benign 0.07
R4247:Grasp UTSW 15 101224537 missense possibly damaging 0.55
R5040:Grasp UTSW 15 101229042 missense probably damaging 1.00
R5117:Grasp UTSW 15 101230537 missense probably damaging 1.00
R7290:Grasp UTSW 15 101231538 missense probably damaging 1.00
Posted On2013-03-25