Mutagenetix > Incidental Mutation

Incidental Mutation 'R1710:Fat4'

190401

Institutional Source

Beutler Lab

Gene Symbol

Fat4

Ensembl Gene

ENSMUSG00000046743

Gene Name

FAT atypical cadherin 4

Synonyms

6030410K14Rik

MMRRC Submission

039743-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1710 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

38886940-39011985 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 38951155 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Asparagine at position 1901 (T1901N)

Ref Sequence

ENSEMBL: ENSMUSP00000061836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061260]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000061260
AA Change: T1901N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000061836
Gene: ENSMUSG00000046743
AA Change: T1901N

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
CA	60	133	4.09e-7	SMART
CA	157	248	4.51e-18	SMART
CA	272	351	7.66e-30	SMART
CA	380	473	2.55e-17	SMART
CA	497	580	8.27e-26	SMART
CA	605	687	6.46e-28	SMART
CA	711	791	1e-24	SMART
CA	815	891	3.78e-20	SMART
CA	915	994	8.6e-24	SMART
CA	1018	1098	7.09e-25	SMART
CA	1122	1208	6.78e-22	SMART
CA	1232	1313	2.63e-28	SMART
CA	1337	1418	7.25e-31	SMART
CA	1442	1527	4.58e-19	SMART
CA	1550	1629	4.52e-9	SMART
CA	1651	1738	1.3e-9	SMART
CA	1762	1839	2.01e-24	SMART
CA	1863	1942	3.11e-21	SMART
CA	1966	2049	5.85e-26	SMART
CA	2072	2152	1.88e-29	SMART
CA	2176	2257	3.06e-29	SMART
CA	2282	2362	2.61e-23	SMART
CA	2386	2466	2.99e-32	SMART
CA	2490	2568	9.92e-6	SMART
CA	2588	2669	6.58e-20	SMART
CA	2692	2773	7.25e-31	SMART
CA	2796	2872	1.69e-22	SMART
CA	2896	2983	3.16e-22	SMART
CA	3007	3089	1.01e-15	SMART
CA	3113	3194	1.25e-25	SMART
CA	3218	3298	7e-15	SMART
CA	3322	3405	3.96e-14	SMART
CA	3428	3510	3.41e-27	SMART
CA	3532	3614	5.64e-19	SMART
EGF	3807	3862	1.78e-2	SMART
EGF_CA	3864	3900	2.36e-16	SMART
EGF_CA	3902	3938	7.99e-14	SMART
EGF	3943	3976	1.24e-1	SMART
LamG	3996	4144	4.08e-19	SMART
EGF	4167	4200	5.88e-3	SMART
LamG	4244	4375	1.76e-23	SMART
EGF	4430	4464	1.41e-5	SMART
low complexity region	4514	4526	N/A	INTRINSIC
low complexity region	4533	4550	N/A	INTRINSIC
low complexity region	4840	4849	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129182

Meta Mutation Damage Score

0.2384

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.5%

Validation Efficiency

98% (117/120)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous inactivation of this gene leads to neonatal lethality, reduced birth body size, curly tails, kyphosis, small lungs, renal cysts, and defects in sternum and vertebrae morphology, neural tube width, cochlear elongation, stereocilia orientation, kidney development, and intestinal elongation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 114 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503L19Rik	A	T	18: 70,468,063 (GRCm38)	S249R	possibly damaging	Het
9130409I23Rik	A	G	1: 181,051,319 (GRCm38)	M1V	probably null	Het
Acadvl	T	A	11: 70,010,355 (GRCm38)	I638F	probably damaging	Het
Acnat2	T	A	4: 49,380,587 (GRCm38)	T264S	probably benign	Het
Acrv1	C	T	9: 36,694,255 (GRCm38)	Q33*	probably null	Het
Actrt3	T	A	3: 30,599,752 (GRCm38)	N33I	probably damaging	Het
Alyref2	T	C	1: 171,503,600 (GRCm38)		probably benign	Het
Ank2	C	A	3: 126,933,060 (GRCm38)	E3712*	probably null	Het
Ano7	C	A	1: 93,385,624 (GRCm38)	H161Q	probably benign	Het
Arhgap10	C	A	8: 77,358,587 (GRCm38)	E451*	probably null	Het
Arhgap29	T	A	3: 122,008,080 (GRCm38)	Y748N	probably damaging	Het
Arhgap45	C	T	10: 80,018,098 (GRCm38)	Q149*	probably null	Het
Asap2	C	T	12: 21,224,392 (GRCm38)	H371Y	probably damaging	Het
Atp6v1b1	T	C	6: 83,758,390 (GRCm38)	I480T	probably benign	Het
BC034090	T	C	1: 155,225,864 (GRCm38)	D218G	possibly damaging	Het
Brdt	A	T	5: 107,343,584 (GRCm38)	D74V	probably damaging	Het
C4b	A	G	17: 34,743,664 (GRCm38)		probably benign	Het
Card11	T	A	5: 140,902,905 (GRCm38)	K233*	probably null	Het
Catip	T	G	1: 74,362,770 (GRCm38)	F35V	possibly damaging	Het
Ccdc13	C	T	9: 121,819,581 (GRCm38)	G247R	probably damaging	Het
Cep97	T	A	16: 55,915,022 (GRCm38)	D471V	probably damaging	Het
Col17a1	A	T	19: 47,670,931 (GRCm38)	L403Q	probably damaging	Het
Crbn	A	G	6: 106,790,945 (GRCm38)	S194P	possibly damaging	Het
Dhx58	T	A	11: 100,703,574 (GRCm38)	H97L	probably benign	Het
Dnah8	T	C	17: 30,854,940 (GRCm38)	I4528T	probably damaging	Het
Dpyd	A	G	3: 118,610,443 (GRCm38)		probably null	Het
Entpd1	A	T	19: 40,726,236 (GRCm38)	Q263L	probably benign	Het
Esyt3	T	C	9: 99,336,191 (GRCm38)	I130M	probably benign	Het
Etv1	T	C	12: 38,852,262 (GRCm38)	F264S	probably benign	Het
Fam189a2	A	T	19: 23,979,695 (GRCm38)	I317N	probably damaging	Het
Fam196b	T	A	11: 34,404,263 (GRCm38)		probably null	Het
Fam45a	A	G	19: 60,817,583 (GRCm38)	Y102C	probably damaging	Het
Fat1	T	C	8: 45,010,482 (GRCm38)	S1354P	probably benign	Het
Fbxw13	C	T	9: 109,181,518 (GRCm38)	V351I	probably damaging	Het
Fmo3	A	G	1: 162,967,787 (GRCm38)	F160L	possibly damaging	Het
Fyb2	T	A	4: 105,003,916 (GRCm38)	D592E	probably damaging	Het
Gjb4	T	C	4: 127,351,870 (GRCm38)	M93V	possibly damaging	Het
Gm5089	C	A	14: 122,436,154 (GRCm38)	G52*	probably null	Het
Gm6401	G	T	14: 41,966,883 (GRCm38)	N76K	probably benign	Het
Gm6871	A	G	7: 41,546,477 (GRCm38)	S279P	probably damaging	Het
Gtf2ird2	T	G	5: 134,211,240 (GRCm38)	V301G	probably benign	Het
H2bfm	A	C	X: 136,927,467 (GRCm38)	D35A	unknown	Het
Hfm1	T	C	5: 106,896,003 (GRCm38)	E589G	probably damaging	Het
Hfm1	A	G	5: 106,880,514 (GRCm38)	F817L	probably damaging	Het
Hivep2	A	T	10: 14,129,505 (GRCm38)	K616*	probably null	Het
Hmcn1	A	T	1: 150,675,984 (GRCm38)	I2623N	probably damaging	Het
Igf2bp3	G	T	6: 49,105,631 (GRCm38)	A339E	probably damaging	Het
Irx4	T	C	13: 73,267,638 (GRCm38)	I182T	possibly damaging	Het
Jcad	T	C	18: 4,674,511 (GRCm38)	S758P	probably damaging	Het
Klhdc4	A	T	8: 121,799,487 (GRCm38)	Y304*	probably null	Het
Lama1	A	G	17: 67,753,791 (GRCm38)	I705V	probably benign	Het
Mbd5	A	G	2: 49,257,032 (GRCm38)	N418S	probably benign	Het
Mmp21	C	T	7: 133,677,285 (GRCm38)	V279I	probably damaging	Het
Morc4	A	C	X: 139,854,530 (GRCm38)	C272W	probably damaging	Het
Mrm1	A	T	11: 84,818,692 (GRCm38)	C180S	probably damaging	Het
Ncor1	T	C	11: 62,423,005 (GRCm38)	D103G	probably damaging	Het
Ndrg4	A	G	8: 95,710,686 (GRCm38)	D251G	probably damaging	Het
Ndufaf5	T	C	2: 140,193,602 (GRCm38)	V246A	possibly damaging	Het
Noa1	T	C	5: 77,309,725 (GRCm38)	E111G	possibly damaging	Het
Nod1	C	A	6: 54,944,059 (GRCm38)	V425F	probably damaging	Het
Nos1	A	T	5: 117,895,919 (GRCm38)	I369F	probably damaging	Het
Nsun5	C	T	5: 135,371,316 (GRCm38)	H98Y	probably damaging	Het
Olfr1013	C	T	2: 85,769,855 (GRCm38)	T18I	probably benign	Het
Olfr172	A	G	16: 58,761,141 (GRCm38)	F12L	probably benign	Het
Olfr292	G	A	7: 86,695,110 (GRCm38)	R218H	probably benign	Het
Olfr726	C	G	14: 50,084,370 (GRCm38)	V104L	probably benign	Het
Olfr933	A	G	9: 38,975,906 (GRCm38)	I77V	probably damaging	Het
Olfr945	T	C	9: 39,258,571 (GRCm38)	I37V	probably benign	Het
Optn	G	A	2: 5,053,130 (GRCm38)	T76I	possibly damaging	Het
Oscar	C	T	7: 3,611,856 (GRCm38)	W22*	probably null	Het
Palmd	T	A	3: 116,923,657 (GRCm38)	Y397F	probably damaging	Het
Plk1	A	G	7: 122,168,898 (GRCm38)	D447G	probably damaging	Het
Plscr5	T	A	9: 92,205,528 (GRCm38)	N183K	probably damaging	Het
Polk	T	G	13: 96,489,204 (GRCm38)	D364A	probably damaging	Het
Polr3d	T	C	14: 70,443,010 (GRCm38)	T36A	probably benign	Het
Ppp1r10	A	T	17: 35,926,536 (GRCm38)	R199S	probably damaging	Het
Prkcz	C	T	4: 155,262,512 (GRCm38)	D388N	probably damaging	Het
Prrc2b	T	C	2: 32,212,222 (GRCm38)	L769P	probably damaging	Het
Psg26	A	G	7: 18,480,041 (GRCm38)	V232A	probably damaging	Het
Pth2r	T	C	1: 65,336,838 (GRCm38)	V85A	possibly damaging	Het
Rbpms2	T	C	9: 65,659,212 (GRCm38)		probably benign	Het
Reps1	A	T	10: 18,118,950 (GRCm38)	D514V	possibly damaging	Het
Riok3	G	T	18: 12,142,961 (GRCm38)	R238L	probably benign	Het
Rnf19a	T	C	15: 36,244,207 (GRCm38)	Q569R	probably damaging	Het
Rorb	G	A	19: 18,960,501 (GRCm38)	T267I	probably damaging	Het
Rrm2b	A	T	15: 37,929,096 (GRCm38)	M70K	probably damaging	Het
Rsf1	A	T	7: 97,662,349 (GRCm38)	E762V	possibly damaging	Het
S100a2	G	A	3: 90,591,392 (GRCm38)	V67I	probably benign	Het
Skp1a	T	A	11: 52,242,615 (GRCm38)	D42E	probably benign	Het
Slc22a4	A	T	11: 54,027,975 (GRCm38)	M1K	probably null	Het
Slc2a9	T	A	5: 38,382,044 (GRCm38)	Q371L	probably damaging	Het
Slc4a9	A	G	18: 36,532,022 (GRCm38)	T475A	probably benign	Het
Slc8b1	A	G	5: 120,519,652 (GRCm38)	N60S	probably damaging	Het
Slx4	A	C	16: 3,999,158 (GRCm38)	D66E	probably benign	Het
Smg8	A	T	11: 87,086,287 (GRCm38)	I156N	probably damaging	Het
Snx25	A	T	8: 46,116,207 (GRCm38)	C218S	possibly damaging	Het
Sulf2	T	C	2: 166,079,072 (GRCm38)	I784V	probably benign	Het
Tatdn2	G	T	6: 113,697,927 (GRCm38)	R72L	possibly damaging	Het
Tbc1d8	T	C	1: 39,406,837 (GRCm38)	D91G	possibly damaging	Het
Tifab	C	A	13: 56,176,620 (GRCm38)	R3S	probably benign	Het
Tm4sf1	A	T	3: 57,292,883 (GRCm38)	S104T	probably damaging	Het
Tmem132e	T	A	11: 82,443,517 (GRCm38)	I618N	probably damaging	Het
Ttc7b	A	T	12: 100,403,408 (GRCm38)	D367E	probably damaging	Het
Txlnb	A	G	10: 17,843,455 (GRCm38)	D678G	possibly damaging	Het
Vgll4	A	G	6: 114,957,934 (GRCm38)		probably null	Het
Vmn2r124	A	T	17: 18,061,925 (GRCm38)		probably benign	Het
Vmn2r2	T	C	3: 64,117,399 (GRCm38)	D587G	probably benign	Het
Vmn2r84	G	A	10: 130,391,099 (GRCm38)	A290V	probably benign	Het
Vps13c	T	A	9: 67,911,529 (GRCm38)	S1077R	probably benign	Het
Vsig10	C	T	5: 117,351,654 (GRCm38)	A495V	probably benign	Het
Vwa3a	A	T	7: 120,804,031 (GRCm38)		probably null	Het
Zfhx2	C	A	14: 55,065,998 (GRCm38)	A1510S	possibly damaging	Het
Zfp142	T	A	1: 74,572,230 (GRCm38)	D601V	probably damaging	Het
Zfp62	T	C	11: 49,217,683 (GRCm38)	I867T	probably benign	Het

Other mutations in Fat4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Fat4	APN	3	38,982,249 (GRCm38)	missense	probably damaging	1.00
IGL00509:Fat4	APN	3	38,889,039 (GRCm38)	missense	probably damaging	1.00
IGL00698:Fat4	APN	3	38,981,145 (GRCm38)	missense	probably benign	0.17
IGL00934:Fat4	APN	3	38,890,673 (GRCm38)	missense	probably damaging	1.00
IGL01063:Fat4	APN	3	38,890,579 (GRCm38)	missense	possibly damaging	0.80
IGL01123:Fat4	APN	3	38,957,269 (GRCm38)	missense	probably benign	0.00
IGL01313:Fat4	APN	3	39,007,201 (GRCm38)	missense	possibly damaging	0.53
IGL01328:Fat4	APN	3	38,980,658 (GRCm38)	missense	probably damaging	1.00
IGL01328:Fat4	APN	3	38,889,991 (GRCm38)	missense	probably damaging	1.00
IGL01374:Fat4	APN	3	38,887,498 (GRCm38)	missense	probably damaging	1.00
IGL01412:Fat4	APN	3	38,891,181 (GRCm38)	missense	probably benign	0.09
IGL01472:Fat4	APN	3	38,888,070 (GRCm38)	missense	probably damaging	1.00
IGL01514:Fat4	APN	3	38,949,534 (GRCm38)	missense	possibly damaging	0.89
IGL01548:Fat4	APN	3	39,009,257 (GRCm38)	missense	probably damaging	1.00
IGL01548:Fat4	APN	3	38,887,758 (GRCm38)	missense	probably damaging	0.99
IGL01576:Fat4	APN	3	38,888,947 (GRCm38)	missense	probably damaging	1.00
IGL01591:Fat4	APN	3	39,010,375 (GRCm38)	nonsense	probably null
IGL01626:Fat4	APN	3	38,951,032 (GRCm38)	missense	probably damaging	1.00
IGL01746:Fat4	APN	3	38,991,731 (GRCm38)	nonsense	probably null
IGL01800:Fat4	APN	3	38,981,729 (GRCm38)	missense	probably damaging	0.99
IGL01815:Fat4	APN	3	38,888,773 (GRCm38)	missense	probably damaging	1.00
IGL01863:Fat4	APN	3	38,970,619 (GRCm38)	splice site	probably benign
IGL01917:Fat4	APN	3	38,889,730 (GRCm38)	missense	possibly damaging	0.89
IGL01936:Fat4	APN	3	38,979,774 (GRCm38)	missense	probably benign	0.10
IGL02060:Fat4	APN	3	39,010,271 (GRCm38)	missense	probably damaging	1.00
IGL02103:Fat4	APN	3	38,889,199 (GRCm38)	missense	probably damaging	0.97
IGL02119:Fat4	APN	3	38,982,939 (GRCm38)	missense	probably benign	0.10
IGL02124:Fat4	APN	3	38,888,404 (GRCm38)	missense	probably damaging	1.00
IGL02164:Fat4	APN	3	38,996,205 (GRCm38)	critical splice donor site	probably null
IGL02182:Fat4	APN	3	38,890,546 (GRCm38)	missense	probably damaging	1.00
IGL02207:Fat4	APN	3	38,951,263 (GRCm38)	missense	probably benign	0.16
IGL02210:Fat4	APN	3	38,891,853 (GRCm38)	missense	probably benign	0.01
IGL02257:Fat4	APN	3	39,001,139 (GRCm38)	missense	probably benign	0.09
IGL02271:Fat4	APN	3	38,979,919 (GRCm38)	missense	probably benign	0.18
IGL02305:Fat4	APN	3	39,009,988 (GRCm38)	missense	probably damaging	1.00
IGL02314:Fat4	APN	3	38,887,630 (GRCm38)	missense	probably damaging	1.00
IGL02455:Fat4	APN	3	38,951,131 (GRCm38)	missense	possibly damaging	0.48
IGL02468:Fat4	APN	3	38,983,046 (GRCm38)	missense	probably benign
IGL02478:Fat4	APN	3	38,888,215 (GRCm38)	missense	probably damaging	1.00
IGL02480:Fat4	APN	3	39,010,430 (GRCm38)	missense	probably damaging	1.00
IGL02487:Fat4	APN	3	38,887,245 (GRCm38)	missense	probably damaging	1.00
IGL02632:Fat4	APN	3	39,002,764 (GRCm38)	missense	probably benign	0.04
IGL02665:Fat4	APN	3	39,002,836 (GRCm38)	missense	probably benign	0.08
IGL02674:Fat4	APN	3	38,983,337 (GRCm38)	missense	probably benign	0.35
IGL02692:Fat4	APN	3	38,951,086 (GRCm38)	missense	probably damaging	1.00
IGL02710:Fat4	APN	3	38,890,595 (GRCm38)	missense	probably damaging	1.00
IGL02803:Fat4	APN	3	38,889,295 (GRCm38)	missense	probably damaging	1.00
IGL02834:Fat4	APN	3	38,956,744 (GRCm38)	missense	probably damaging	1.00
IGL02891:Fat4	APN	3	38,951,273 (GRCm38)	missense	probably damaging	1.00
IGL02982:Fat4	APN	3	38,890,843 (GRCm38)	missense	probably damaging	1.00
IGL02993:Fat4	APN	3	38,957,155 (GRCm38)	missense	probably damaging	1.00
IGL02996:Fat4	APN	3	38,958,525 (GRCm38)	missense	probably damaging	1.00
IGL03029:Fat4	APN	3	38,982,591 (GRCm38)	missense	possibly damaging	0.46
IGL03124:Fat4	APN	3	38,981,552 (GRCm38)	missense	possibly damaging	0.61
IGL03144:Fat4	APN	3	38,956,859 (GRCm38)	missense	possibly damaging	0.68
IGL03149:Fat4	APN	3	38,991,685 (GRCm38)	missense	probably damaging	1.00
IGL03169:Fat4	APN	3	38,957,398 (GRCm38)	missense	probably benign	0.02
IGL03190:Fat4	APN	3	38,981,241 (GRCm38)	missense	probably damaging	1.00
IGL03272:Fat4	APN	3	39,009,703 (GRCm38)	missense	probably benign
IGL03371:Fat4	APN	3	38,983,187 (GRCm38)	missense	possibly damaging	0.65
IGL03372:Fat4	APN	3	38,889,134 (GRCm38)	missense	possibly damaging	0.88
IGL03388:Fat4	APN	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
IGL03394:Fat4	APN	3	38,892,019 (GRCm38)	missense	probably damaging	0.99
IGL03394:Fat4	APN	3	39,009,364 (GRCm38)	missense	probably damaging	1.00
IGL03405:Fat4	APN	3	38,958,450 (GRCm38)	missense	probably benign	0.02
IGL03410:Fat4	APN	3	38,891,176 (GRCm38)	missense	probably damaging	1.00
Asahi	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
Expulsion	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
heineken	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
schlitz	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
PIT4696001:Fat4	UTSW	3	38,982,357 (GRCm38)	missense	probably damaging	0.98
PIT4696001:Fat4	UTSW	3	38,889,004 (GRCm38)	missense	probably benign	0.04
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0078:Fat4	UTSW	3	38,888,931 (GRCm38)	missense	probably benign	0.35
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0201:Fat4	UTSW	3	38,891,596 (GRCm38)	missense	probably damaging	0.99
R0280:Fat4	UTSW	3	38,890,816 (GRCm38)	missense	probably benign
R0357:Fat4	UTSW	3	38,891,227 (GRCm38)	missense	probably damaging	1.00
R0409:Fat4	UTSW	3	38,977,413 (GRCm38)	missense	probably damaging	1.00
R0498:Fat4	UTSW	3	38,980,637 (GRCm38)	missense	probably benign	0.00
R0502:Fat4	UTSW	3	39,002,924 (GRCm38)	missense	probably damaging	0.98
R0506:Fat4	UTSW	3	38,888,314 (GRCm38)	missense	probably benign	0.00
R0532:Fat4	UTSW	3	38,981,721 (GRCm38)	missense	probably benign	0.02
R0616:Fat4	UTSW	3	38,942,870 (GRCm38)	missense	probably damaging	1.00
R0630:Fat4	UTSW	3	39,000,172 (GRCm38)	missense	probably damaging	1.00
R0678:Fat4	UTSW	3	38,889,694 (GRCm38)	missense	probably damaging	1.00
R0685:Fat4	UTSW	3	39,001,178 (GRCm38)	missense	probably benign
R0729:Fat4	UTSW	3	39,000,295 (GRCm38)	splice site	probably benign
R0748:Fat4	UTSW	3	38,887,828 (GRCm38)	missense	possibly damaging	0.67
R0811:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0812:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0830:Fat4	UTSW	3	38,999,109 (GRCm38)	missense	probably benign	0.26
R0841:Fat4	UTSW	3	38,995,998 (GRCm38)	missense	probably damaging	0.99
R0884:Fat4	UTSW	3	38,982,858 (GRCm38)	missense	possibly damaging	0.89
R1056:Fat4	UTSW	3	38,891,392 (GRCm38)	missense	probably damaging	1.00
R1066:Fat4	UTSW	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
R1078:Fat4	UTSW	3	38,983,086 (GRCm38)	missense	probably benign	0.10
R1084:Fat4	UTSW	3	38,979,825 (GRCm38)	missense	possibly damaging	0.88
R1118:Fat4	UTSW	3	38,982,942 (GRCm38)	missense	possibly damaging	0.88
R1213:Fat4	UTSW	3	38,890,371 (GRCm38)	missense	probably benign	0.01
R1418:Fat4	UTSW	3	38,890,813 (GRCm38)	missense	probably damaging	1.00
R1475:Fat4	UTSW	3	38,888,323 (GRCm38)	missense	probably damaging	1.00
R1487:Fat4	UTSW	3	38,995,917 (GRCm38)	missense	possibly damaging	0.77
R1511:Fat4	UTSW	3	38,983,076 (GRCm38)	missense	probably damaging	0.97
R1534:Fat4	UTSW	3	38,890,089 (GRCm38)	missense	probably damaging	1.00
R1558:Fat4	UTSW	3	38,888,986 (GRCm38)	missense	probably damaging	1.00
R1586:Fat4	UTSW	3	38,888,860 (GRCm38)	missense	probably damaging	1.00
R1592:Fat4	UTSW	3	39,007,177 (GRCm38)	missense	probably damaging	0.99
R1655:Fat4	UTSW	3	38,957,318 (GRCm38)	missense	probably damaging	0.97
R1662:Fat4	UTSW	3	38,980,779 (GRCm38)	missense	probably damaging	1.00
R1731:Fat4	UTSW	3	38,891,310 (GRCm38)	missense	probably damaging	1.00
R1761:Fat4	UTSW	3	38,887,489 (GRCm38)	missense	possibly damaging	0.61
R1770:Fat4	UTSW	3	39,010,268 (GRCm38)	missense	probably damaging	1.00
R1828:Fat4	UTSW	3	38,983,458 (GRCm38)	missense	probably damaging	1.00
R1835:Fat4	UTSW	3	38,983,571 (GRCm38)	missense	probably benign	0.00
R1846:Fat4	UTSW	3	38,982,383 (GRCm38)	missense	probably benign	0.00
R1861:Fat4	UTSW	3	39,010,484 (GRCm38)	missense	probably benign	0.09
R1871:Fat4	UTSW	3	38,981,072 (GRCm38)	missense	possibly damaging	0.63
R1981:Fat4	UTSW	3	38,991,664 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,996,090 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,887,115 (GRCm38)	missense	probably benign
R2056:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2058:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2059:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2070:Fat4	UTSW	3	39,010,655 (GRCm38)	missense	probably benign	0.00
R2078:Fat4	UTSW	3	38,889,673 (GRCm38)	missense	probably damaging	1.00
R2114:Fat4	UTSW	3	38,981,484 (GRCm38)	missense	probably benign	0.01
R2135:Fat4	UTSW	3	38,980,733 (GRCm38)	missense	probably damaging	0.98
R2152:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2153:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2154:Fat4	UTSW	3	38,887,539 (GRCm38)	missense	probably damaging	1.00
R2196:Fat4	UTSW	3	38,981,417 (GRCm38)	missense	probably benign	0.23
R2211:Fat4	UTSW	3	38,891,527 (GRCm38)	missense	possibly damaging	0.77
R2219:Fat4	UTSW	3	39,010,215 (GRCm38)	missense	probably damaging	1.00
R2247:Fat4	UTSW	3	38,892,049 (GRCm38)	missense	probably damaging	1.00
R2263:Fat4	UTSW	3	38,888,989 (GRCm38)	missense	possibly damaging	0.93
R2264:Fat4	UTSW	3	38,890,422 (GRCm38)	missense	probably benign	0.25
R2274:Fat4	UTSW	3	38,995,899 (GRCm38)	missense	possibly damaging	0.47
R2337:Fat4	UTSW	3	38,980,011 (GRCm38)	missense	probably damaging	1.00
R2343:Fat4	UTSW	3	38,957,105 (GRCm38)	missense	probably damaging	0.97
R2365:Fat4	UTSW	3	38,980,419 (GRCm38)	missense	probably benign
R2412:Fat4	UTSW	3	38,957,072 (GRCm38)	missense	probably benign	0.05
R2883:Fat4	UTSW	3	38,980,804 (GRCm38)	missense	probably damaging	1.00
R2942:Fat4	UTSW	3	38,982,336 (GRCm38)	missense	probably damaging	1.00
R2989:Fat4	UTSW	3	39,007,153 (GRCm38)	missense	probably benign
R3103:Fat4	UTSW	3	38,891,940 (GRCm38)	missense	probably benign	0.03
R3158:Fat4	UTSW	3	38,890,791 (GRCm38)	missense	possibly damaging	0.87
R3800:Fat4	UTSW	3	38,981,274 (GRCm38)	missense	possibly damaging	0.48
R3808:Fat4	UTSW	3	38,982,438 (GRCm38)	missense	possibly damaging	0.52
R3848:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3850:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3957:Fat4	UTSW	3	38,982,346 (GRCm38)	missense	probably benign
R4065:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign	0.13
R4078:Fat4	UTSW	3	38,980,020 (GRCm38)	missense	probably damaging	1.00
R4096:Fat4	UTSW	3	38,887,875 (GRCm38)	missense	possibly damaging	0.46
R4161:Fat4	UTSW	3	38,942,809 (GRCm38)	missense	possibly damaging	0.95
R4273:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R4285:Fat4	UTSW	3	38,889,171 (GRCm38)	missense	probably benign	0.00
R4288:Fat4	UTSW	3	38,891,763 (GRCm38)	missense	probably damaging	1.00
R4407:Fat4	UTSW	3	38,958,540 (GRCm38)	missense	probably benign	0.05
R4528:Fat4	UTSW	3	38,891,294 (GRCm38)	missense	probably benign	0.01
R4547:Fat4	UTSW	3	38,951,283 (GRCm38)	missense	probably damaging	1.00
R4681:Fat4	UTSW	3	38,887,342 (GRCm38)	missense	probably damaging	1.00
R4826:Fat4	UTSW	3	38,982,957 (GRCm38)	missense	probably damaging	1.00
R4855:Fat4	UTSW	3	38,888,317 (GRCm38)	missense	probably benign
R4871:Fat4	UTSW	3	38,891,605 (GRCm38)	missense	probably damaging	1.00
R4897:Fat4	UTSW	3	38,980,632 (GRCm38)	missense	probably damaging	1.00
R4928:Fat4	UTSW	3	39,010,465 (GRCm38)	missense	probably damaging	1.00
R4932:Fat4	UTSW	3	39,007,203 (GRCm38)	missense	probably benign	0.00
R4941:Fat4	UTSW	3	38,957,452 (GRCm38)	missense	probably damaging	1.00
R4943:Fat4	UTSW	3	38,980,173 (GRCm38)	missense	probably benign	0.19
R4959:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R4973:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R5098:Fat4	UTSW	3	38,888,289 (GRCm38)	missense	probably benign	0.34
R5163:Fat4	UTSW	3	38,980,797 (GRCm38)	missense	probably damaging	1.00
R5213:Fat4	UTSW	3	38,980,191 (GRCm38)	missense	possibly damaging	0.56
R5328:Fat4	UTSW	3	38,956,868 (GRCm38)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	39,010,378 (GRCm38)	missense	probably benign	0.44
R5337:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R5363:Fat4	UTSW	3	38,888,005 (GRCm38)	missense	probably damaging	1.00
R5380:Fat4	UTSW	3	38,888,864 (GRCm38)	missense	probably damaging	1.00
R5384:Fat4	UTSW	3	38,995,946 (GRCm38)	missense	possibly damaging	0.87
R5422:Fat4	UTSW	3	38,887,245 (GRCm38)	missense	possibly damaging	0.92
R5436:Fat4	UTSW	3	38,891,346 (GRCm38)	missense	probably benign	0.00
R5443:Fat4	UTSW	3	39,010,370 (GRCm38)	missense	probably damaging	1.00
R5501:Fat4	UTSW	3	38,887,215 (GRCm38)	missense	probably benign	0.09
R5571:Fat4	UTSW	3	39,010,274 (GRCm38)	missense	probably damaging	1.00
R5625:Fat4	UTSW	3	38,888,934 (GRCm38)	missense	possibly damaging	0.78
R5652:Fat4	UTSW	3	39,002,968 (GRCm38)	missense	probably damaging	0.99
R5725:Fat4	UTSW	3	38,889,625 (GRCm38)	missense	probably damaging	1.00
R5735:Fat4	UTSW	3	38,949,576 (GRCm38)	missense	probably damaging	1.00
R5739:Fat4	UTSW	3	38,983,134 (GRCm38)	missense	probably benign	0.01
R5766:Fat4	UTSW	3	38,889,468 (GRCm38)	missense	probably damaging	1.00
R5780:Fat4	UTSW	3	38,980,955 (GRCm38)	missense	probably damaging	0.96
R5811:Fat4	UTSW	3	38,891,787 (GRCm38)	missense	probably damaging	1.00
R5829:Fat4	UTSW	3	39,007,305 (GRCm38)	missense	probably damaging	1.00
R5879:Fat4	UTSW	3	38,887,336 (GRCm38)	missense	probably benign
R5933:Fat4	UTSW	3	38,951,375 (GRCm38)	critical splice donor site	probably null
R5938:Fat4	UTSW	3	38,951,239 (GRCm38)	missense	probably damaging	1.00
R5940:Fat4	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
R5945:Fat4	UTSW	3	38,983,206 (GRCm38)	missense	probably benign	0.19
R5963:Fat4	UTSW	3	39,010,547 (GRCm38)	missense	probably damaging	1.00
R6077:Fat4	UTSW	3	39,002,802 (GRCm38)	missense	probably damaging	1.00
R6158:Fat4	UTSW	3	38,983,262 (GRCm38)	missense	possibly damaging	0.95
R6246:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	1.00
R6253:Fat4	UTSW	3	38,951,356 (GRCm38)	missense	probably damaging	0.99
R6259:Fat4	UTSW	3	39,007,246 (GRCm38)	missense	probably benign	0.18
R6295:Fat4	UTSW	3	39,007,080 (GRCm38)	splice site	probably null
R6387:Fat4	UTSW	3	38,983,785 (GRCm38)	missense	probably damaging	1.00
R6390:Fat4	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
R6456:Fat4	UTSW	3	38,983,979 (GRCm38)	missense	possibly damaging	0.90
R6493:Fat4	UTSW	3	38,890,887 (GRCm38)	missense	probably damaging	1.00
R6500:Fat4	UTSW	3	38,981,269 (GRCm38)	nonsense	probably null
R6503:Fat4	UTSW	3	38,982,257 (GRCm38)	missense	probably benign	0.00
R6519:Fat4	UTSW	3	39,002,871 (GRCm38)	missense	probably benign
R6566:Fat4	UTSW	3	38,957,126 (GRCm38)	missense	possibly damaging	0.78
R6576:Fat4	UTSW	3	38,979,690 (GRCm38)	missense	probably benign
R6590:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6658:Fat4	UTSW	3	38,942,928 (GRCm38)	missense	probably benign	0.01
R6662:Fat4	UTSW	3	38,956,821 (GRCm38)	missense	possibly damaging	0.95
R6690:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6807:Fat4	UTSW	3	38,982,440 (GRCm38)	missense	probably benign	0.18
R6823:Fat4	UTSW	3	38,983,939 (GRCm38)	missense	probably benign	0.05
R6824:Fat4	UTSW	3	38,957,525 (GRCm38)	missense	probably benign	0.00
R6830:Fat4	UTSW	3	38,981,817 (GRCm38)	missense	probably benign	0.00
R6925:Fat4	UTSW	3	38,996,204 (GRCm38)	critical splice donor site	probably null
R6948:Fat4	UTSW	3	39,009,446 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,995,971 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,981,775 (GRCm38)	missense	probably damaging	1.00
R7017:Fat4	UTSW	3	38,891,543 (GRCm38)	missense	probably benign
R7030:Fat4	UTSW	3	38,981,958 (GRCm38)	missense	probably damaging	1.00
R7044:Fat4	UTSW	3	39,010,811 (GRCm38)	missense	probably benign	0.02
R7044:Fat4	UTSW	3	39,010,810 (GRCm38)	missense	probably benign
R7045:Fat4	UTSW	3	38,888,601 (GRCm38)	missense	probably benign	0.01
R7094:Fat4	UTSW	3	38,889,874 (GRCm38)	missense	probably damaging	1.00
R7111:Fat4	UTSW	3	39,010,533 (GRCm38)	missense	probably damaging	1.00
R7130:Fat4	UTSW	3	38,980,787 (GRCm38)	missense	probably damaging	0.99
R7168:Fat4	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
R7192:Fat4	UTSW	3	38,980,464 (GRCm38)	missense	probably benign	0.04
R7194:Fat4	UTSW	3	38,983,895 (GRCm38)	missense	probably damaging	1.00
R7194:Fat4	UTSW	3	38,888,884 (GRCm38)	missense	probably damaging	1.00
R7199:Fat4	UTSW	3	38,977,362 (GRCm38)	missense	probably damaging	0.98
R7213:Fat4	UTSW	3	38,999,087 (GRCm38)	missense	possibly damaging	0.63
R7216:Fat4	UTSW	3	38,891,043 (GRCm38)	missense	probably damaging	1.00
R7225:Fat4	UTSW	3	38,980,176 (GRCm38)	missense	possibly damaging	0.50
R7238:Fat4	UTSW	3	38,890,413 (GRCm38)	missense	probably benign	0.31
R7239:Fat4	UTSW	3	38,983,840 (GRCm38)	missense	possibly damaging	0.85
R7283:Fat4	UTSW	3	38,889,693 (GRCm38)	missense	probably damaging	1.00
R7296:Fat4	UTSW	3	38,889,145 (GRCm38)	nonsense	probably null
R7372:Fat4	UTSW	3	38,890,209 (GRCm38)	missense	probably damaging	1.00
R7400:Fat4	UTSW	3	38,887,924 (GRCm38)	missense	probably damaging	1.00
R7419:Fat4	UTSW	3	39,000,236 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	39,009,644 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	38,887,450 (GRCm38)	missense	probably damaging	0.97
R7431:Fat4	UTSW	3	39,009,157 (GRCm38)	missense	possibly damaging	0.80
R7486:Fat4	UTSW	3	38,957,427 (GRCm38)	nonsense	probably null
R7501:Fat4	UTSW	3	38,958,448 (GRCm38)	nonsense	probably null
R7533:Fat4	UTSW	3	39,007,257 (GRCm38)	missense	probably benign	0.43
R7542:Fat4	UTSW	3	38,981,621 (GRCm38)	missense	possibly damaging	0.64
R7542:Fat4	UTSW	3	38,981,355 (GRCm38)	missense	possibly damaging	0.56
R7548:Fat4	UTSW	3	38,981,114 (GRCm38)	missense	probably benign	0.13
R7567:Fat4	UTSW	3	38,889,336 (GRCm38)	missense	probably damaging	1.00
R7644:Fat4	UTSW	3	39,010,241 (GRCm38)	missense	possibly damaging	0.64
R7660:Fat4	UTSW	3	38,981,160 (GRCm38)	missense	probably benign
R7665:Fat4	UTSW	3	38,889,178 (GRCm38)	missense	probably benign	0.00
R7676:Fat4	UTSW	3	38,891,697 (GRCm38)	missense	probably damaging	0.98
R7832:Fat4	UTSW	3	39,001,204 (GRCm38)	missense	probably benign	0.00
R7848:Fat4	UTSW	3	38,887,851 (GRCm38)	missense	probably benign
R7883:Fat4	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
R7892:Fat4	UTSW	3	38,949,439 (GRCm38)	critical splice acceptor site	probably null
R7904:Fat4	UTSW	3	38,887,541 (GRCm38)	missense	probably damaging	1.00
R7952:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	0.98
R8015:Fat4	UTSW	3	38,981,916 (GRCm38)	missense	possibly damaging	0.79
R8040:Fat4	UTSW	3	38,981,666 (GRCm38)	missense	probably damaging	1.00
R8142:Fat4	UTSW	3	38,891,203 (GRCm38)	missense	probably damaging	1.00
R8151:Fat4	UTSW	3	38,892,054 (GRCm38)	missense	probably damaging	0.99
R8163:Fat4	UTSW	3	38,979,732 (GRCm38)	missense	possibly damaging	0.88
R8317:Fat4	UTSW	3	38,958,510 (GRCm38)	missense	possibly damaging	0.80
R8413:Fat4	UTSW	3	39,008,979 (GRCm38)	critical splice acceptor site	probably null
R8447:Fat4	UTSW	3	38,979,675 (GRCm38)	missense	possibly damaging	0.88
R8458:Fat4	UTSW	3	38,981,553 (GRCm38)	missense	probably benign	0.25
R8509:Fat4	UTSW	3	38,981,903 (GRCm38)	missense	probably benign
R8543:Fat4	UTSW	3	38,977,494 (GRCm38)	missense	probably damaging	1.00
R8679:Fat4	UTSW	3	39,010,693 (GRCm38)	missense	probably damaging	1.00
R8726:Fat4	UTSW	3	39,010,498 (GRCm38)	missense	probably damaging	1.00
R8743:Fat4	UTSW	3	38,888,443 (GRCm38)	missense	probably benign	0.16
R8751:Fat4	UTSW	3	38,891,853 (GRCm38)	missense	probably benign	0.01
R8779:Fat4	UTSW	3	38,979,749 (GRCm38)	missense	probably damaging	1.00
R8797:Fat4	UTSW	3	38,999,129 (GRCm38)	missense	probably benign	0.01
R8860:Fat4	UTSW	3	38,892,120 (GRCm38)	missense	probably benign	0.26
R8955:Fat4	UTSW	3	38,983,629 (GRCm38)	missense	probably benign	0.01
R9053:Fat4	UTSW	3	38,887,175 (GRCm38)	nonsense	probably null
R9071:Fat4	UTSW	3	38,983,449 (GRCm38)	missense	probably benign	0.29
R9088:Fat4	UTSW	3	39,007,299 (GRCm38)	missense	probably benign	0.02
R9100:Fat4	UTSW	3	39,010,654 (GRCm38)	missense
R9180:Fat4	UTSW	3	38,888,407 (GRCm38)	missense	possibly damaging	0.78
R9184:Fat4	UTSW	3	38,982,443 (GRCm38)	missense	probably damaging	0.99
R9201:Fat4	UTSW	3	38,890,930 (GRCm38)	missense	probably damaging	1.00
R9206:Fat4	UTSW	3	39,009,241 (GRCm38)	missense	probably damaging	0.99
R9268:Fat4	UTSW	3	38,888,247 (GRCm38)	missense	probably damaging	1.00
R9278:Fat4	UTSW	3	38,891,022 (GRCm38)	missense	probably benign	0.44
R9287:Fat4	UTSW	3	38,891,632 (GRCm38)	missense	probably damaging	0.98
R9355:Fat4	UTSW	3	38,981,898 (GRCm38)	missense	probably damaging	1.00
R9437:Fat4	UTSW	3	38,891,268 (GRCm38)	missense	probably benign	0.00
R9455:Fat4	UTSW	3	38,891,263 (GRCm38)	missense
R9456:Fat4	UTSW	3	38,888,422 (GRCm38)	missense	possibly damaging	0.50
R9476:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9510:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9511:Fat4	UTSW	3	38,980,653 (GRCm38)	missense	probably damaging	0.98
R9540:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign
R9568:Fat4	UTSW	3	38,892,007 (GRCm38)	missense	probably damaging	1.00
R9646:Fat4	UTSW	3	38,981,664 (GRCm38)	missense	probably damaging	1.00
R9683:Fat4	UTSW	3	38,889,183 (GRCm38)	missense	possibly damaging	0.52
R9711:Fat4	UTSW	3	39,001,225 (GRCm38)	missense	probably benign	0.00
X0017:Fat4	UTSW	3	39,009,106 (GRCm38)	missense	probably benign	0.00
X0019:Fat4	UTSW	3	38,981,040 (GRCm38)	missense	probably damaging	1.00
X0020:Fat4	UTSW	3	39,000,151 (GRCm38)	missense	probably damaging	1.00
X0024:Fat4	UTSW	3	38,942,902 (GRCm38)	missense	probably benign	0.43
X0064:Fat4	UTSW	3	38,970,752 (GRCm38)	missense	probably damaging	1.00
Z1088:Fat4	UTSW	3	38,958,492 (GRCm38)	missense	probably benign	0.00
Z1088:Fat4	UTSW	3	38,887,050 (GRCm38)	missense	possibly damaging	0.88
Z1176:Fat4	UTSW	3	38,983,815 (GRCm38)	missense	probably damaging	1.00
Z1176:Fat4	UTSW	3	38,983,359 (GRCm38)	missense	probably benign	0.00
Z1177:Fat4	UTSW	3	38,890,347 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,888,584 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,981,838 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGGTGTGTCAGTCTAGAAGTCAGC -3'
(R):5'- CCGAGAGGCAGGTTTTCCATTAAGG -3'

Sequencing Primer
(F):5'- AGTCAGCAGAATACTGTGCTCTC -3'
(R):5'- CCATTAAGGATGTGCTGTAGGAG -3'

Posted On

2014-05-14