Mutagenetix > Incidental Mutation

Incidental Mutation 'R1714:Ralgapa1'

190880

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, 2310003F20Rik, Tulip1

MMRRC Submission

039747-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.755) question?

Stock #

R1714 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

55602896-55821167 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 55642389 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 1928 (V1928A)

Ref Sequence

ENSEMBL: ENSMUSP00000151498 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

probably damaging
Transcript: ENSMUST00000085385
AA Change: V1928A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027
AA Change: V1928A

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000110687
AA Change: V1928A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027
AA Change: V1928A

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000219323

Predicted Effect

probably damaging
Transcript: ENSMUST00000219432
AA Change: V1975A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

unknown
Transcript: ENSMUST00000219451
AA Change: V20A

Predicted Effect

probably damaging
Transcript: ENSMUST00000220367
AA Change: V1928A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000226244
AA Change: V2384A

PolyPhen 2 Score 0.455 (Sensitivity: 0.89; Specificity: 0.90)

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	C	T	13: 77,316,360 (GRCm38)	T892I	possibly damaging	Het
Abcb11	A	C	2: 69,306,581 (GRCm38)	F179V	probably damaging	Het
Adgrg6	G	T	10: 14,439,770 (GRCm38)	Q597K	possibly damaging	Het
Ankmy1	G	T	1: 92,885,194 (GRCm38)	Y464*	probably null	Het
Apba1	G	A	19: 23,944,952 (GRCm38)	E795K	possibly damaging	Het
Aqp12	T	A	1: 93,006,959 (GRCm38)	V186D	possibly damaging	Het
Brd8	A	T	18: 34,609,833 (GRCm38)	S253R	probably damaging	Het
Cdc42se2	A	T	11: 54,740,286 (GRCm38)	S2R	possibly damaging	Het
Chd9	A	C	8: 91,034,225 (GRCm38)		probably benign	Het
Clk4	C	T	11: 51,280,418 (GRCm38)	H219Y	probably damaging	Het
Cngb1	A	G	8: 95,257,931 (GRCm38)	Y417H	probably damaging	Het
Cr2	A	T	1: 195,151,686 (GRCm38)	F932I	possibly damaging	Het
Cxxc1	C	A	18: 74,219,863 (GRCm38)	R415S	probably damaging	Het
Dclk2	G	A	3: 86,906,093 (GRCm38)	A182V	probably benign	Het
Ddx11	T	G	17: 66,148,759 (GRCm38)	W718G	probably damaging	Het
Dmd	A	C	X: 83,964,750 (GRCm38)	T2069P	probably benign	Het
Dnaic1	T	C	4: 41,632,164 (GRCm38)	F533L	probably benign	Het
Dnajc5b	A	T	3: 19,579,101 (GRCm38)	R163*	probably null	Het
Dynll2	T	A	11: 87,984,012 (GRCm38)		probably null	Het
Ercc5	A	G	1: 44,167,339 (GRCm38)	T471A	probably benign	Het
Fan1	T	C	7: 64,366,687 (GRCm38)	D563G	probably benign	Het
Fbxo34	A	G	14: 47,529,201 (GRCm38)	Y6C	probably damaging	Het
Fcrl5	T	A	3: 87,446,406 (GRCm38)	S353T	probably damaging	Het
Gabrb3	A	T	7: 57,765,428 (GRCm38)	Y82F	probably damaging	Het
Gm20939	C	A	17: 94,875,806 (GRCm38)	P157T	probably damaging	Het
Haus3	A	T	5: 34,163,697 (GRCm38)	H468Q	probably benign	Het
Hist1h2ba	T	C	13: 23,933,952 (GRCm38)	T69A	probably benign	Het
Il20ra	T	C	10: 19,755,828 (GRCm38)	V259A	probably damaging	Het
Isg15	A	T	4: 156,199,957 (GRCm38)	V38E	probably damaging	Het
Kcnq3	A	G	15: 66,000,063 (GRCm38)	S586P	probably benign	Het
Kl	A	T	5: 150,953,333 (GRCm38)	Y206F	probably benign	Het
Klk1b24	C	A	7: 44,191,515 (GRCm38)	D122E	probably damaging	Het
Kmt2d	A	C	15: 98,862,950 (GRCm38)	S840A	unknown	Het
Krt34	A	G	11: 100,040,127 (GRCm38)	S150P	possibly damaging	Het
Lamc3	A	G	2: 31,940,757 (GRCm38)	K1502R	probably benign	Het
Letm1	G	T	5: 33,760,884 (GRCm38)	R306S	possibly damaging	Het
Lnx1	C	T	5: 74,607,737 (GRCm38)	G397S	probably null	Het
Lrrc8c	A	G	5: 105,607,291 (GRCm38)	T311A	possibly damaging	Het
Mpeg1	A	T	19: 12,462,834 (GRCm38)	D552V	probably damaging	Het
Myh11	C	T	16: 14,236,368 (GRCm38)		probably null	Het
Ndufa9	T	C	6: 126,822,191 (GRCm38)		probably null	Het
Olfr1289	A	T	2: 111,483,663 (GRCm38)	I78F	probably damaging	Het
Olfr1507	A	C	14: 52,490,414 (GRCm38)		probably null	Het
Olfr281	G	A	15: 98,456,733 (GRCm38)	C141Y	probably damaging	Het
Polr3d	A	C	14: 70,441,315 (GRCm38)	M117R	possibly damaging	Het
Ppp5c	T	C	7: 17,008,703 (GRCm38)	I237V	probably benign	Het
Prrc2c	G	A	1: 162,677,376 (GRCm38)	T2632M	probably damaging	Het
Ptprg	C	A	14: 12,213,697 (GRCm38)	Q1022K	probably damaging	Het
Pus10	T	A	11: 23,725,542 (GRCm38)	H471Q	probably damaging	Het
Rbpms2	ACTGCTGCTGCTGCTGC	ACTGCTGCTGCTGCTGCTGC	9: 65,651,666 (GRCm38)		probably benign	Het
Rbpms2	CACT	CACTACT	9: 65,651,665 (GRCm38)		probably benign	Het
Rfpl4	T	G	7: 5,110,358 (GRCm38)	T269P	probably benign	Het
Rgs10	A	G	7: 128,403,222 (GRCm38)	V72A	probably damaging	Het
Rsph1	T	C	17: 31,255,216 (GRCm38)	N289S	probably benign	Het
Sbk2	T	C	7: 4,963,122 (GRCm38)	D21G	probably benign	Het
Shbg	A	T	11: 69,617,157 (GRCm38)	D127E	possibly damaging	Het
Spag16	A	G	1: 69,843,005 (GRCm38)	E52G	probably damaging	Het
Ssh2	G	A	11: 77,454,024 (GRCm38)	G945D	possibly damaging	Het
Sstr3	A	T	15: 78,540,273 (GRCm38)	D91E	probably damaging	Het
Syne2	C	A	12: 76,054,939 (GRCm38)	A669E	probably benign	Het
Traf3	A	G	12: 111,242,473 (GRCm38)	I109V	probably benign	Het
Ube2j1	A	G	4: 33,049,886 (GRCm38)	T295A	probably damaging	Het
Usp46	A	G	5: 74,003,167 (GRCm38)	V276A	probably benign	Het
Vmn1r200	T	C	13: 22,395,470 (GRCm38)	S139P	possibly damaging	Het
Ydjc	G	A	16: 17,147,799 (GRCm38)	V143M	probably damaging	Het
Zfp169	T	C	13: 48,498,854 (GRCm38)	E29G	probably benign	Het
Zfp292	A	G	4: 34,808,935 (GRCm38)	S1370P	probably damaging	Het
Zfp763	C	T	17: 33,019,617 (GRCm38)	D185N	probably damaging	Het
Zfp827	A	T	8: 79,060,573 (GRCm38)	N123Y	probably damaging	Het
Zfp979	A	T	4: 147,613,985 (GRCm38)	I89N	probably damaging	Het
Zfr2	T	C	10: 81,244,749 (GRCm38)	L419P	probably damaging	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,722,773 (GRCm38)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,747,185 (GRCm38)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,702,452 (GRCm38)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,709,575 (GRCm38)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,642,359 (GRCm38)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,642,348 (GRCm38)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,777,316 (GRCm38)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,719,657 (GRCm38)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,642,477 (GRCm38)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,708,077 (GRCm38)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,642,449 (GRCm38)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,712,665 (GRCm38)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,673,507 (GRCm38)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,676,417 (GRCm38)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,717,069 (GRCm38)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,757,951 (GRCm38)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
bantam	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
wickedwarlock	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,795,653 (GRCm38)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,695,157 (GRCm38)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,786,263 (GRCm38)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,739,505 (GRCm38)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
R0361:Ralgapa1	UTSW	12	55,676,569 (GRCm38)	missense	possibly damaging	0.93
R0385:Ralgapa1	UTSW	12	55,677,038 (GRCm38)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,708,067 (GRCm38)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,689,791 (GRCm38)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,676,765 (GRCm38)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,782,885 (GRCm38)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,795,698 (GRCm38)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,665,663 (GRCm38)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,676,581 (GRCm38)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,782,777 (GRCm38)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,782,777 (GRCm38)	splice site	probably benign
R0863:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,762,661 (GRCm38)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,707,978 (GRCm38)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,676,926 (GRCm38)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,741,480 (GRCm38)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,684,524 (GRCm38)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,770,703 (GRCm38)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,741,536 (GRCm38)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,762,603 (GRCm38)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,676,767 (GRCm38)	missense	possibly damaging	0.79
R1832:Ralgapa1	UTSW	12	55,757,967 (GRCm38)	missense	probably benign	0.03
R1870:Ralgapa1	UTSW	12	55,677,032 (GRCm38)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,786,322 (GRCm38)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,677,026 (GRCm38)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,695,160 (GRCm38)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,776,188 (GRCm38)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,677,124 (GRCm38)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,820,755 (GRCm38)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,659,137 (GRCm38)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,770,613 (GRCm38)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,695,143 (GRCm38)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,659,130 (GRCm38)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,698,767 (GRCm38)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,795,701 (GRCm38)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,739,330 (GRCm38)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,677,276 (GRCm38)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,693,458 (GRCm38)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,712,748 (GRCm38)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,794,993 (GRCm38)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,676,437 (GRCm38)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,698,803 (GRCm38)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,762,574 (GRCm38)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,718,114 (GRCm38)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,612,723 (GRCm38)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,665,674 (GRCm38)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,776,152 (GRCm38)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,758,032 (GRCm38)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,676,797 (GRCm38)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,719,623 (GRCm38)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,676,710 (GRCm38)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,612,738 (GRCm38)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,820,766 (GRCm38)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,677,113 (GRCm38)	missense	probably damaging	1.00
R5950:Ralgapa1	UTSW	12	55,738,265 (GRCm38)	missense	possibly damaging	0.58
R5980:Ralgapa1	UTSW	12	55,770,616 (GRCm38)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,684,042 (GRCm38)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,757,924 (GRCm38)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,747,146 (GRCm38)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,683,910 (GRCm38)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,719,661 (GRCm38)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,738,319 (GRCm38)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,762,727 (GRCm38)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,604,273 (GRCm38)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,786,212 (GRCm38)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,776,191 (GRCm38)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,758,059 (GRCm38)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,820,723 (GRCm38)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,721,576 (GRCm38)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,604,191 (GRCm38)	missense	probably damaging	1.00
R7254:Ralgapa1	UTSW	12	55,695,193 (GRCm38)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,709,004 (GRCm38)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,712,672 (GRCm38)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,718,228 (GRCm38)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,659,143 (GRCm38)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,709,556 (GRCm38)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,709,555 (GRCm38)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,741,513 (GRCm38)	missense	probably benign
R7763:Ralgapa1	UTSW	12	55,757,955 (GRCm38)	missense	probably benign	0.28
R7791:Ralgapa1	UTSW	12	55,741,519 (GRCm38)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,719,628 (GRCm38)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,612,638 (GRCm38)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,747,149 (GRCm38)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,697,878 (GRCm38)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,702,457 (GRCm38)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,782,846 (GRCm38)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,722,914 (GRCm38)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,741,523 (GRCm38)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,659,062 (GRCm38)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,676,518 (GRCm38)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,739,413 (GRCm38)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,738,217 (GRCm38)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,738,316 (GRCm38)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,702,560 (GRCm38)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,820,761 (GRCm38)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,677,006 (GRCm38)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,605,529 (GRCm38)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,676,566 (GRCm38)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,735,096 (GRCm38)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,709,058 (GRCm38)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9446:Ralgapa1	UTSW	12	55,708,023 (GRCm38)	missense	probably damaging	0.97
R9684:Ralgapa1	UTSW	12	55,612,700 (GRCm38)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,709,080 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTACAAGAACTTTCACTCAGGCACAG -3'
(R):5'- CAGCCACTTCCCTTCTAGGTTGATG -3'

Sequencing Primer
(F):5'- GCACAGCCTAGCTGTCTC -3'
(R):5'- ACTCTGCTCACATGGCTTTT -3'

Posted On

2014-05-14