Mutagenetix > Incidental Mutation

Incidental Mutation 'R1718:Vmn2r61'

191200

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r61

Ensembl Gene

ENSMUSG00000090967

Gene Name

vomeronasal 2, receptor 61

Synonyms

EG637873, Casr-rs2, Gprc2a-rs2

MMRRC Submission

039751-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.123) question?

Stock #

R1718 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42260053-42300755 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 42300697 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 847 (D847G)

Ref Sequence

ENSEMBL: ENSMUSP00000129576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166131]

AlphaFold

L7N2B8

Predicted Effect

probably benign
Transcript: ENSMUST00000166131
AA Change: D847G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000129576
Gene: ENSMUSG00000090967
AA Change: D847G

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	4e-42	PFAM
Pfam:NCD3G	514	567	1.9e-21	PFAM
Pfam:7tm_3	600	835	6.2e-52	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173044

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.6%
20x: 93.5%

Validation Efficiency

100% (69/69)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	G	T	13: 77,245,370		probably benign	Het
Acot3	T	G	12: 84,053,943		probably null	Het
Acox1	A	T	11: 116,174,682	C523*	probably null	Het
Adamts19	G	A	18: 58,972,825	C764Y	probably damaging	Het
Agrn	GCTCT	GCTCTCT	4: 156,166,519		probably null	Het
Apob	A	G	12: 8,016,087	K4319R	probably benign	Het
AU016765	A	C	17: 64,555,438		noncoding transcript	Het
Bpifb1	T	A	2: 154,213,983		probably null	Het
Btn2a2	A	G	13: 23,481,936	V242A	probably benign	Het
Camta1	A	G	4: 151,084,024	S1281P	probably benign	Het
Ccdc116	T	C	16: 17,141,908	K306E	probably benign	Het
Cemip	A	G	7: 83,935,658	V1350A	probably benign	Het
Clip2	A	T	5: 134,502,929	L674*	probably null	Het
Cyp2d12	T	A	15: 82,558,050	D244E	probably benign	Het
Cyp4x1	A	G	4: 115,111,670	V379A	possibly damaging	Het
Dnah9	T	A	11: 66,168,079	H130L	possibly damaging	Het
Enpp7	A	G	11: 118,990,983	Y318C	probably damaging	Het
Fras1	A	T	5: 96,554,889		probably null	Het
Glra3	G	T	8: 55,940,907	A18S	probably benign	Het
Gm28042	T	A	2: 120,036,391	S172T	possibly damaging	Het
Gm7808	T	A	9: 19,928,003		probably benign	Het
Gm8909	A	G	17: 36,161,784		probably benign	Het
Gpr61	C	T	3: 108,150,380	V322M	possibly damaging	Het
Hapln3	A	G	7: 79,123,450	V15A	unknown	Het
Ip6k1	G	A	9: 108,040,996	E77K	possibly damaging	Het
Klk1b4	A	G	7: 44,209,672	Y38C	probably damaging	Het
Lrrfip1	A	G	1: 91,115,555	K561E	probably damaging	Het
Map3k1	A	G	13: 111,755,419	C1101R	probably benign	Het
Mcoln2	A	G	3: 146,190,474		probably benign	Het
Mfsd2b	G	A	12: 4,869,037	T73I	probably damaging	Het
Mfsd4b5	C	T	10: 39,975,203	V19I	probably benign	Het
Mgme1	T	A	2: 144,272,318	D113E	probably benign	Het
Mki67	A	G	7: 135,695,494	S2604P	probably damaging	Het
Mob3c	A	G	4: 115,831,644	I125V	probably benign	Het
Mrps9	G	A	1: 42,903,399	R339H	probably damaging	Het
Ndst1	T	C	18: 60,707,803	D269G	probably damaging	Het
Nedd9	T	C	13: 41,338,926	N30S	probably damaging	Het
Notch4	G	A	17: 34,576,763		probably benign	Het
Olfr1095	A	T	2: 86,851,187	N170K	probably benign	Het
Olfr250	A	G	9: 38,367,594	D6G	probably benign	Het
Olfr877	G	A	9: 37,855,453	V212I	probably benign	Het
Olfr995	T	C	2: 85,438,805	M118V	probably benign	Het
Papss1	C	A	3: 131,619,185	R447S	probably damaging	Het
Pla2g4a	C	T	1: 149,871,523		probably benign	Het
Rab11fip2	A	G	19: 59,935,649	F266L	probably damaging	Het
Ralgapb	T	A	2: 158,443,280	Y554*	probably null	Het
Rem2	T	C	14: 54,479,150	V240A	probably damaging	Het
Retsat	T	C	6: 72,602,671	V143A	probably benign	Het
Rnf141	G	T	7: 110,821,273	Q175K	probably damaging	Het
Rtcb	C	A	10: 85,942,017	G431V	probably damaging	Het
Slc7a6os	A	G	8: 106,204,339	W222R	probably damaging	Het
Smarcc2	T	C	10: 128,468,998		probably benign	Het
Smchd1	A	T	17: 71,448,833	Y218N	possibly damaging	Het
Sp110	G	A	1: 85,594,385	H66Y	probably benign	Het
Speg	G	A	1: 75,417,863	E1739K	probably benign	Het
Speg	A	G	1: 75,421,744	Q1945R	possibly damaging	Het
Sprtn	T	C	8: 124,898,357	V67A	probably damaging	Het
Tktl2	G	A	8: 66,512,347	V186M	probably damaging	Het
Tnks1bp1	G	T	2: 85,071,738	E997D	probably benign	Het
Tti1	A	T	2: 158,008,224	V365E	probably benign	Het
Tulp4	A	G	17: 6,222,440	I590V	probably benign	Het
Zfp184	A	G	13: 21,959,272	T383A	possibly damaging	Het
Zik1	T	A	7: 10,492,341	E33V	probably damaging	Het
Zik1	C	A	7: 10,492,342	E33*	probably null	Het

Other mutations in Vmn2r61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r61	APN	7	42300751	missense	possibly damaging	0.96
IGL00824:Vmn2r61	APN	7	42267014	missense	probably benign	0.03
IGL00903:Vmn2r61	APN	7	42300511	missense	probably damaging	1.00
IGL01125:Vmn2r61	APN	7	42260126	missense	probably damaging	1.00
IGL01393:Vmn2r61	APN	7	42266834	missense	probably benign	0.08
IGL01712:Vmn2r61	APN	7	42260237	missense	probably damaging	0.98
IGL01822:Vmn2r61	APN	7	42300706	missense	probably benign	0.18
IGL01835:Vmn2r61	APN	7	42300591	missense	probably benign	0.12
IGL01844:Vmn2r61	APN	7	42260215	missense	probably benign	0.00
IGL01953:Vmn2r61	APN	7	42300189	missense	probably damaging	1.00
IGL02032:Vmn2r61	APN	7	42300042	missense	probably damaging	0.99
IGL02054:Vmn2r61	APN	7	42276734	critical splice donor site	probably null
IGL02569:Vmn2r61	APN	7	42276646	missense	probably damaging	1.00
IGL02697:Vmn2r61	APN	7	42275468	missense	possibly damaging	0.55
IGL02958:Vmn2r61	APN	7	42299937	missense	probably benign
IGL03290:Vmn2r61	APN	7	42265984	missense	probably benign	0.00
IGL03337:Vmn2r61	APN	7	42267085	missense	possibly damaging	0.58
IGL03369:Vmn2r61	APN	7	42260093	missense	probably benign
IGL03402:Vmn2r61	APN	7	42260255	missense	probably benign
R0026:Vmn2r61	UTSW	7	42275474	missense	possibly damaging	0.64
R0319:Vmn2r61	UTSW	7	42300517	missense	probably damaging	0.99
R0433:Vmn2r61	UTSW	7	42265911	missense	probably benign	0.02
R0555:Vmn2r61	UTSW	7	42266018	missense	probably benign	0.02
R0691:Vmn2r61	UTSW	7	42300420	missense	probably damaging	1.00
R1701:Vmn2r61	UTSW	7	42300511	missense	probably damaging	1.00
R1835:Vmn2r61	UTSW	7	42266652	nonsense	probably null
R1920:Vmn2r61	UTSW	7	42300286	missense	possibly damaging	0.73
R2069:Vmn2r61	UTSW	7	42300001	missense	probably benign	0.06
R2326:Vmn2r61	UTSW	7	42266863	missense	probably damaging	1.00
R2402:Vmn2r61	UTSW	7	42300105	missense	possibly damaging	0.90
R3103:Vmn2r61	UTSW	7	42266643	missense	possibly damaging	0.73
R3107:Vmn2r61	UTSW	7	42267067	missense	possibly damaging	0.82
R4426:Vmn2r61	UTSW	7	42300733	missense	probably benign
R4426:Vmn2r61	UTSW	7	42300735	missense	probably benign
R4484:Vmn2r61	UTSW	7	42300696	missense	probably benign
R4748:Vmn2r61	UTSW	7	42267141	missense	probably damaging	0.96
R4835:Vmn2r61	UTSW	7	42267035	missense	possibly damaging	0.52
R4863:Vmn2r61	UTSW	7	42300708	missense	probably benign	0.03
R4923:Vmn2r61	UTSW	7	42267096	missense	probably damaging	1.00
R4968:Vmn2r61	UTSW	7	42300054	missense	probably benign	0.14
R5114:Vmn2r61	UTSW	7	42300529	missense	possibly damaging	0.92
R5297:Vmn2r61	UTSW	7	42260222	missense	probably benign
R5497:Vmn2r61	UTSW	7	42275482	missense	possibly damaging	0.95
R5508:Vmn2r61	UTSW	7	42266818	missense	possibly damaging	0.52
R5587:Vmn2r61	UTSW	7	42300487	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	42267253	missense	probably benign	0.00
R5615:Vmn2r61	UTSW	7	42300493	missense	probably damaging	1.00
R5782:Vmn2r61	UTSW	7	42299829	missense	probably damaging	1.00
R6136:Vmn2r61	UTSW	7	42267031	missense	probably damaging	1.00
R6207:Vmn2r61	UTSW	7	42260192	missense	probably benign	0.01
R6265:Vmn2r61	UTSW	7	42266491	missense	probably benign	0.01
R6272:Vmn2r61	UTSW	7	42299818	missense	probably damaging	1.00
R6355:Vmn2r61	UTSW	7	42267235	missense	probably benign	0.00
R6469:Vmn2r61	UTSW	7	42265859	nonsense	probably null
R6554:Vmn2r61	UTSW	7	42276715	missense	probably damaging	1.00
R6699:Vmn2r61	UTSW	7	42300156	missense	probably benign
R6768:Vmn2r61	UTSW	7	42300324	missense	probably damaging	1.00
R6824:Vmn2r61	UTSW	7	42299979	missense	probably benign	0.10
R6930:Vmn2r61	UTSW	7	42299940	missense	probably benign	0.02
R7053:Vmn2r61	UTSW	7	42267133	missense	probably damaging	0.96
R7238:Vmn2r61	UTSW	7	42267205	missense	possibly damaging	0.73
R7332:Vmn2r61	UTSW	7	42260110	missense	probably benign	0.00
R7359:Vmn2r61	UTSW	7	42265983	missense	probably benign	0.11
R7553:Vmn2r61	UTSW	7	42266781	missense	not run
R7710:Vmn2r61	UTSW	7	42267048	missense	probably damaging	1.00
R7732:Vmn2r61	UTSW	7	42266673	missense	probably benign
R7839:Vmn2r61	UTSW	7	42266608	missense	probably damaging	0.97
R7916:Vmn2r61	UTSW	7	42300511	missense	probably damaging	1.00
R8026:Vmn2r61	UTSW	7	42266717	missense	probably benign	0.02
R8440:Vmn2r61	UTSW	7	42266656	missense	probably benign	0.02
R8499:Vmn2r61	UTSW	7	42300276	missense	probably damaging	0.99
R8771:Vmn2r61	UTSW	7	42266770	missense	probably damaging	0.99
R8847:Vmn2r61	UTSW	7	42300586	missense	probably damaging	1.00
R8986:Vmn2r61	UTSW	7	42265901	nonsense	probably null
R9290:Vmn2r61	UTSW	7	42265961	missense	probably benign	0.27
R9311:Vmn2r61	UTSW	7	42300668	missense	possibly damaging	0.92
R9324:Vmn2r61	UTSW	7	42267195	missense	probably benign	0.00
R9476:Vmn2r61	UTSW	7	42300169	missense	probably damaging	1.00
R9521:Vmn2r61	UTSW	7	42267202	missense	probably damaging	0.99
R9619:Vmn2r61	UTSW	7	42276712	missense	probably damaging	0.98
R9729:Vmn2r61	UTSW	7	42300493	missense	probably damaging	1.00
Z1088:Vmn2r61	UTSW	7	42299964	missense	possibly damaging	0.93
Z1176:Vmn2r61	UTSW	7	42260161	missense	possibly damaging	0.46
Z1176:Vmn2r61	UTSW	7	42266742	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CCTGACACCTTCAATGAGGCCAAG -3'
(R):5'- TGCTGACACATCTTCCACAGGAAC -3'

Sequencing Primer
(F):5'- GAGGCCAAGTTCCTTTCATTCAG -3'
(R):5'- TTATGGGACACTAGGCCAAC -3'

Posted On

2014-05-14