Incidental Mutation 'R1719:Cyp4a32'
ID191263
Institutional Source Beutler Lab
Gene Symbol Cyp4a32
Ensembl Gene ENSMUSG00000063929
Gene Namecytochrome P450, family 4, subfamily a, polypeptide 32
SynonymsOTTMUSG00000008689
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.073) question?
Stock #R1719 (G1)
Quality Score225
Status Not validated
Chromosome4
Chromosomal Location115600969-115621602 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 115611308 bp
ZygosityHeterozygous
Amino Acid Change Valine to Alanine at position 329 (V329A)
Ref Sequence ENSEMBL: ENSMUSP00000081369 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000084342]
Predicted Effect possibly damaging
Transcript: ENSMUST00000084342
AA Change: V329A

PolyPhen 2 Score 0.611 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000081369
Gene: ENSMUSG00000063929
AA Change: V329A

DomainStartEndE-ValueType
transmembrane domain 15 32 N/A INTRINSIC
Pfam:p450 51 504 1.3e-133 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000129918
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.3%
  • 10x: 96.2%
  • 20x: 92.3%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 84 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4931406P16Rik A T 7: 34,248,206 M459K probably damaging Het
A1cf A T 19: 31,927,126 K263M probably damaging Het
Adam26a G A 8: 43,570,036 T139M possibly damaging Het
Agrp G T 8: 105,566,835 T106K probably damaging Het
Akap9 C T 5: 3,957,645 Q238* probably null Het
Ankrd6 A T 4: 32,828,774 V85E probably damaging Het
Ap2b1 C A 11: 83,324,604 P125T probably damaging Het
Arhgef7 C A 8: 11,808,713 probably null Het
Cblc A T 7: 19,790,474 D280E probably benign Het
Cdk11b T C 4: 155,648,397 probably benign Het
Cela2a G T 4: 141,817,946 F239L probably damaging Het
Cfap57 A T 4: 118,606,631 C342S probably benign Het
Clca4a C A 3: 144,963,755 W345L probably damaging Het
Col6a5 T C 9: 105,931,293 D852G unknown Het
Dcaf8 C T 1: 172,175,495 P287S probably damaging Het
Dmxl1 T A 18: 49,934,637 D2654E probably damaging Het
Dpep1 A T 8: 123,200,747 I347F possibly damaging Het
Dtnb T A 12: 3,643,936 Y56* probably null Het
Duox1 T C 2: 122,338,644 Y1182H possibly damaging Het
Dusp10 T A 1: 184,037,225 S129R probably benign Het
Epcam G A 17: 87,642,128 R173Q probably damaging Het
Ephb3 A G 16: 21,220,650 E384G probably damaging Het
Exosc10 A T 4: 148,568,503 D525V probably damaging Het
Fam186a T A 15: 99,942,346 T2006S possibly damaging Het
Fbxw21 T A 9: 109,148,174 T156S possibly damaging Het
Fcrl5 A G 3: 87,457,397 E568G probably damaging Het
Fmn2 C T 1: 174,608,458 probably benign Het
Fut9 A G 4: 25,619,744 F357L possibly damaging Het
Gas2l1 G A 11: 5,064,266 H65Y probably damaging Het
Gjd2 A T 2: 114,013,133 M1K probably null Het
Gm12169 T C 11: 46,526,294 L13P probably damaging Het
Gm884 T A 11: 103,617,071 probably benign Het
Hdgfl3 T C 7: 81,899,684 Y149C probably damaging Het
Hmcn2 T C 2: 31,354,721 V730A probably damaging Het
Inpp4a A T 1: 37,398,799 S223C probably damaging Het
Ip6k1 G A 9: 108,040,996 E77K possibly damaging Het
Knl1 T A 2: 119,071,738 W1307R probably benign Het
Kpna3 A G 14: 61,387,477 L139P probably damaging Het
Lama3 T C 18: 12,479,872 probably null Het
Lin54 G A 5: 100,485,249 P192L possibly damaging Het
Lpo T A 11: 87,809,192 probably null Het
Lrrd1 T G 5: 3,850,483 probably null Het
Nbas T A 12: 13,560,977 probably null Het
Nemp1 G A 10: 127,696,248 G341D probably damaging Het
Nrp1 T C 8: 128,425,885 F192L probably damaging Het
Nufip2 T A 11: 77,693,090 V610E probably damaging Het
Nup160 T G 2: 90,700,436 Y479* probably null Het
Oas1d A T 5: 120,919,962 D323V possibly damaging Het
Olfr125 A G 17: 37,835,353 D118G possibly damaging Het
Olfr1338 A G 4: 118,753,600 W315R possibly damaging Het
Olfr140 T C 2: 90,051,784 Y180C probably damaging Het
Olfr147 G A 9: 38,403,254 V127M possibly damaging Het
Olfr204 T A 16: 59,314,706 R234* probably null Het
Olfr25 A T 9: 38,330,507 T307S probably benign Het
Olfr677 C A 7: 105,056,794 H183N probably damaging Het
Pcm1 T A 8: 41,313,359 M1567K possibly damaging Het
Pdxk A G 10: 78,443,896 V215A probably benign Het
Phf12 T A 11: 78,023,601 L74Q probably damaging Het
Plcg1 A G 2: 160,753,743 E537G probably null Het
Plxna2 C T 1: 194,644,370 P204L possibly damaging Het
Ppp3cb T G 14: 20,524,063 M236L probably benign Het
Qrsl1 A T 10: 43,896,030 S55T probably damaging Het
Rbm5 T C 9: 107,743,913 probably null Het
Sipa1l2 A G 8: 125,444,535 S1403P probably damaging Het
Sis C T 3: 72,965,604 C67Y probably damaging Het
Specc1 A G 11: 62,128,392 I686V possibly damaging Het
Speg G A 1: 75,417,863 E1739K probably benign Het
Sprtn C A 8: 124,901,633 H154Q probably damaging Het
St7l A G 3: 104,870,987 T147A probably benign Het
Stab1 G A 14: 31,146,028 Q1630* probably null Het
Stpg2 A G 3: 139,232,199 D173G probably benign Het
Tcf20 G A 15: 82,852,777 T1491I probably benign Het
Themis2 A T 4: 132,789,649 I180N possibly damaging Het
Tktl2 G A 8: 66,512,347 V186M probably damaging Het
Ttn C T 2: 76,745,634 V24972M probably damaging Het
Ttn A G 2: 76,807,996 V13980A probably damaging Het
Usp5 A T 6: 124,823,460 M286K possibly damaging Het
Vmn2r22 G A 6: 123,637,843 R263C possibly damaging Het
Vmn2r71 T G 7: 85,621,227 C534G probably damaging Het
Wdr60 T C 12: 116,255,912 I137V probably benign Het
Wnk2 C T 13: 49,060,726 S1460N possibly damaging Het
Zfp445 G A 9: 122,852,642 P745S probably damaging Het
Zfp957 C T 14: 79,213,996 G121D probably damaging Het
Zscan4f A G 7: 11,401,327 E220G possibly damaging Het
Other mutations in Cyp4a32
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02225:Cyp4a32 APN 4 115610503 missense probably benign
IGL02546:Cyp4a32 APN 4 115611323 missense probably damaging 0.98
IGL02578:Cyp4a32 APN 4 115609742 missense possibly damaging 0.79
IGL02663:Cyp4a32 APN 4 115610590 missense probably damaging 1.00
IGL02832:Cyp4a32 APN 4 115614621 missense probably damaging 0.99
IGL03283:Cyp4a32 APN 4 115611083 missense possibly damaging 0.79
IGL03357:Cyp4a32 APN 4 115611601 missense probably benign 0.00
IGL03406:Cyp4a32 APN 4 115602303 missense probably benign 0.00
R0379:Cyp4a32 UTSW 4 115621474 missense probably damaging 1.00
R1339:Cyp4a32 UTSW 4 115611563 missense probably damaging 0.98
R1435:Cyp4a32 UTSW 4 115606666 missense probably damaging 0.97
R1445:Cyp4a32 UTSW 4 115602950 nonsense probably null
R1520:Cyp4a32 UTSW 4 115614652 missense probably damaging 0.97
R1587:Cyp4a32 UTSW 4 115610534 missense probably benign 0.06
R1932:Cyp4a32 UTSW 4 115611277 missense possibly damaging 0.78
R4184:Cyp4a32 UTSW 4 115621523 missense possibly damaging 0.94
R4580:Cyp4a32 UTSW 4 115602929 splice site silent
R5004:Cyp4a32 UTSW 4 115601041 missense probably damaging 0.98
R6345:Cyp4a32 UTSW 4 115602363 missense possibly damaging 0.81
R7231:Cyp4a32 UTSW 4 115609697 missense probably damaging 1.00
R7241:Cyp4a32 UTSW 4 115602302 missense probably benign
R7419:Cyp4a32 UTSW 4 115611037 missense probably benign
R7716:Cyp4a32 UTSW 4 115601086 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GACACATGAAGCTACATGGGGCTAC -3'
(R):5'- GTTCTCTGACAATGCCTGGGACAG -3'

Sequencing Primer
(F):5'- GGGCTACTATAGCTCTCAGGTC -3'
(R):5'- CTAGGTGATCCCTGAAATAAAGTCC -3'
Posted On2014-05-14