Mutagenetix > Incidental Mutation

Incidental Mutation 'R1719:Cdk11b'

191269

Institutional Source

Beutler Lab

Gene Symbol

Cdk11b

Ensembl Gene

ENSMUSG00000029062

Gene Name

cyclin-dependent kinase 11B

Synonyms

PITSLRE proteins, CDK11-p110, Cdc2l1, Cdc2l2, CDK11-p46, CDK11-p58

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1719 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

155624854-155649938 bp(+) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to C at 155648397 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000111488 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030937] [ENSMUST00000067081] [ENSMUST00000105598] [ENSMUST00000105600] [ENSMUST00000115821]

AlphaFold

P24788

Predicted Effect

probably benign
Transcript: ENSMUST00000030937

SMART Domains

Protein: ENSMUSP00000030937
Gene: ENSMUSG00000029061

Domain	Start	End	E-Value	Type
low complexity region	19	41	N/A	INTRINSIC
ZnMc	85	256	8.39e-48	SMART
ShKT	255	291	4.06e-10	SMART
IG	307	390	4.53e-2	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000067081
AA Change: I564T

SMART Domains

Protein: ENSMUSP00000070527
Gene: ENSMUSG00000029062
AA Change: I564T

Domain	Start	End	E-Value	Type
low complexity region	19	48	N/A	INTRINSIC
low complexity region	93	112	N/A	INTRINSIC
coiled coil region	123	214	N/A	INTRINSIC
low complexity region	252	259	N/A	INTRINSIC
low complexity region	261	272	N/A	INTRINSIC
coiled coil region	290	337	N/A	INTRINSIC
low complexity region	369	383	N/A	INTRINSIC
S_TKc	427	712	5.05e-93	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000105598
AA Change: I530T

SMART Domains

Protein: ENSMUSP00000101223
Gene: ENSMUSG00000029062
AA Change: I530T

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
low complexity region	59	78	N/A	INTRINSIC
coiled coil region	89	180	N/A	INTRINSIC
low complexity region	218	225	N/A	INTRINSIC
low complexity region	227	238	N/A	INTRINSIC
coiled coil region	256	303	N/A	INTRINSIC
low complexity region	335	349	N/A	INTRINSIC
S_TKc	393	678	5.05e-93	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000105600
AA Change: I564T

SMART Domains

Protein: ENSMUSP00000101225
Gene: ENSMUSG00000029062
AA Change: I564T

Domain	Start	End	E-Value	Type
low complexity region	19	48	N/A	INTRINSIC
low complexity region	93	112	N/A	INTRINSIC
coiled coil region	123	214	N/A	INTRINSIC
low complexity region	252	259	N/A	INTRINSIC
low complexity region	261	272	N/A	INTRINSIC
coiled coil region	290	337	N/A	INTRINSIC
low complexity region	369	383	N/A	INTRINSIC
S_TKc	427	712	5.05e-93	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000115821

SMART Domains

Protein: ENSMUSP00000111488
Gene: ENSMUSG00000073682

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	17	52	2e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130944

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139242

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143196

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155100

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156906

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygous null embryos display embryonic lethality from cell cycle arrest. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931406P16Rik	A	T	7: 34,248,206	M459K	probably damaging	Het
A1cf	A	T	19: 31,927,126	K263M	probably damaging	Het
Adam26a	G	A	8: 43,570,036	T139M	possibly damaging	Het
Agrp	G	T	8: 105,566,835	T106K	probably damaging	Het
Akap9	C	T	5: 3,957,645	Q238*	probably null	Het
Ankrd6	A	T	4: 32,828,774	V85E	probably damaging	Het
Ap2b1	C	A	11: 83,324,604	P125T	probably damaging	Het
Arhgef7	C	A	8: 11,808,713		probably null	Het
Cblc	A	T	7: 19,790,474	D280E	probably benign	Het
Cela2a	G	T	4: 141,817,946	F239L	probably damaging	Het
Cfap57	A	T	4: 118,606,631	C342S	probably benign	Het
Clca4a	C	A	3: 144,963,755	W345L	probably damaging	Het
Col6a5	T	C	9: 105,931,293	D852G	unknown	Het
Cyp4a32	T	C	4: 115,611,308	V329A	possibly damaging	Het
Dcaf8	C	T	1: 172,175,495	P287S	probably damaging	Het
Dmxl1	T	A	18: 49,934,637	D2654E	probably damaging	Het
Dpep1	A	T	8: 123,200,747	I347F	possibly damaging	Het
Dtnb	T	A	12: 3,643,936	Y56*	probably null	Het
Duox1	T	C	2: 122,338,644	Y1182H	possibly damaging	Het
Dusp10	T	A	1: 184,037,225	S129R	probably benign	Het
Epcam	G	A	17: 87,642,128	R173Q	probably damaging	Het
Ephb3	A	G	16: 21,220,650	E384G	probably damaging	Het
Exosc10	A	T	4: 148,568,503	D525V	probably damaging	Het
Fam186a	T	A	15: 99,942,346	T2006S	possibly damaging	Het
Fbxw21	T	A	9: 109,148,174	T156S	possibly damaging	Het
Fcrl5	A	G	3: 87,457,397	E568G	probably damaging	Het
Fmn2	C	T	1: 174,608,458		probably benign	Het
Fut9	A	G	4: 25,619,744	F357L	possibly damaging	Het
Gas2l1	G	A	11: 5,064,266	H65Y	probably damaging	Het
Gjd2	A	T	2: 114,013,133	M1K	probably null	Het
Gm12169	T	C	11: 46,526,294	L13P	probably damaging	Het
Gm884	T	A	11: 103,617,071		probably benign	Het
Hdgfl3	T	C	7: 81,899,684	Y149C	probably damaging	Het
Hmcn2	T	C	2: 31,354,721	V730A	probably damaging	Het
Inpp4a	A	T	1: 37,398,799	S223C	probably damaging	Het
Ip6k1	G	A	9: 108,040,996	E77K	possibly damaging	Het
Knl1	T	A	2: 119,071,738	W1307R	probably benign	Het
Kpna3	A	G	14: 61,387,477	L139P	probably damaging	Het
Lama3	T	C	18: 12,479,872		probably null	Het
Lin54	G	A	5: 100,485,249	P192L	possibly damaging	Het
Lpo	T	A	11: 87,809,192		probably null	Het
Lrrd1	T	G	5: 3,850,483		probably null	Het
Nbas	T	A	12: 13,560,977		probably null	Het
Nemp1	G	A	10: 127,696,248	G341D	probably damaging	Het
Nrp1	T	C	8: 128,425,885	F192L	probably damaging	Het
Nufip2	T	A	11: 77,693,090	V610E	probably damaging	Het
Nup160	T	G	2: 90,700,436	Y479*	probably null	Het
Oas1d	A	T	5: 120,919,962	D323V	possibly damaging	Het
Olfr125	A	G	17: 37,835,353	D118G	possibly damaging	Het
Olfr1338	A	G	4: 118,753,600	W315R	possibly damaging	Het
Olfr140	T	C	2: 90,051,784	Y180C	probably damaging	Het
Olfr147	G	A	9: 38,403,254	V127M	possibly damaging	Het
Olfr204	T	A	16: 59,314,706	R234*	probably null	Het
Olfr25	A	T	9: 38,330,507	T307S	probably benign	Het
Olfr677	C	A	7: 105,056,794	H183N	probably damaging	Het
Pcm1	T	A	8: 41,313,359	M1567K	possibly damaging	Het
Pdxk	A	G	10: 78,443,896	V215A	probably benign	Het
Phf12	T	A	11: 78,023,601	L74Q	probably damaging	Het
Plcg1	A	G	2: 160,753,743	E537G	probably null	Het
Plxna2	C	T	1: 194,644,370	P204L	possibly damaging	Het
Ppp3cb	T	G	14: 20,524,063	M236L	probably benign	Het
Qrsl1	A	T	10: 43,896,030	S55T	probably damaging	Het
Rbm5	T	C	9: 107,743,913		probably null	Het
Sipa1l2	A	G	8: 125,444,535	S1403P	probably damaging	Het
Sis	C	T	3: 72,965,604	C67Y	probably damaging	Het
Specc1	A	G	11: 62,128,392	I686V	possibly damaging	Het
Speg	G	A	1: 75,417,863	E1739K	probably benign	Het
Sprtn	C	A	8: 124,901,633	H154Q	probably damaging	Het
St7l	A	G	3: 104,870,987	T147A	probably benign	Het
Stab1	G	A	14: 31,146,028	Q1630*	probably null	Het
Stpg2	A	G	3: 139,232,199	D173G	probably benign	Het
Tcf20	G	A	15: 82,852,777	T1491I	probably benign	Het
Themis2	A	T	4: 132,789,649	I180N	possibly damaging	Het
Tktl2	G	A	8: 66,512,347	V186M	probably damaging	Het
Ttn	C	T	2: 76,745,634	V24972M	probably damaging	Het
Ttn	A	G	2: 76,807,996	V13980A	probably damaging	Het
Usp5	A	T	6: 124,823,460	M286K	possibly damaging	Het
Vmn2r22	G	A	6: 123,637,843	R263C	possibly damaging	Het
Vmn2r71	T	G	7: 85,621,227	C534G	probably damaging	Het
Wdr60	T	C	12: 116,255,912	I137V	probably benign	Het
Wnk2	C	T	13: 49,060,726	S1460N	possibly damaging	Het
Zfp445	G	A	9: 122,852,642	P745S	probably damaging	Het
Zfp957	C	T	14: 79,213,996	G121D	probably damaging	Het
Zscan4f	A	G	7: 11,401,327	E220G	possibly damaging	Het

Other mutations in Cdk11b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01764:Cdk11b	APN	4	155628803	missense	possibly damaging	0.54
R0071:Cdk11b	UTSW	4	155649423	unclassified	probably benign
R0071:Cdk11b	UTSW	4	155649423	unclassified	probably benign
R0145:Cdk11b	UTSW	4	155641619	intron	probably benign
R0372:Cdk11b	UTSW	4	155641500	intron	probably benign
R0426:Cdk11b	UTSW	4	155642512	intron	probably benign
R0471:Cdk11b	UTSW	4	155647542	unclassified	probably benign
R0627:Cdk11b	UTSW	4	155640772	intron	probably benign
R1475:Cdk11b	UTSW	4	155634217	missense	probably damaging	1.00
R1611:Cdk11b	UTSW	4	155641575	intron	probably benign
R1750:Cdk11b	UTSW	4	155628680	splice site	probably null
R2061:Cdk11b	UTSW	4	155641604	intron	probably benign
R2274:Cdk11b	UTSW	4	155647594	unclassified	probably benign
R2922:Cdk11b	UTSW	4	155640744	intron	probably benign
R3719:Cdk11b	UTSW	4	155626886	missense	probably damaging	1.00
R3917:Cdk11b	UTSW	4	155626801	missense	probably damaging	1.00
R4077:Cdk11b	UTSW	4	155639747	intron	probably benign
R4078:Cdk11b	UTSW	4	155639747	intron	probably benign
R5033:Cdk11b	UTSW	4	155648825	unclassified	probably benign
R5212:Cdk11b	UTSW	4	155638615	splice site	probably null
R5556:Cdk11b	UTSW	4	155634147	nonsense	probably null
R5622:Cdk11b	UTSW	4	155630217	missense	probably damaging	1.00
R5927:Cdk11b	UTSW	4	155648240	unclassified	probably benign
R5975:Cdk11b	UTSW	4	155648240	unclassified	probably benign
R6276:Cdk11b	UTSW	4	155634190	missense	probably benign	0.11
R6278:Cdk11b	UTSW	4	155649603	unclassified	probably benign
R6905:Cdk11b	UTSW	4	155641608	intron	probably benign
R6998:Cdk11b	UTSW	4	155648343	nonsense	probably null
R7021:Cdk11b	UTSW	4	155641567	intron	probably benign
R7062:Cdk11b	UTSW	4	155626811	missense	probably damaging	1.00
R7100:Cdk11b	UTSW	4	155625593	missense	probably damaging	1.00
R7338:Cdk11b	UTSW	4	155647551	missense	unknown
R7811:Cdk11b	UTSW	4	155639902	missense	unknown
R8213:Cdk11b	UTSW	4	155639881	missense	unknown
R8257:Cdk11b	UTSW	4	155647941	missense	unknown
R8696:Cdk11b	UTSW	4	155648322	missense	unknown
R9419:Cdk11b	UTSW	4	155639845	missense	unknown
R9546:Cdk11b	UTSW	4	155649132	missense	unknown
R9628:Cdk11b	UTSW	4	155649697	missense	unknown
R9792:Cdk11b	UTSW	4	155647921	missense	unknown
R9793:Cdk11b	UTSW	4	155647921	missense	unknown
Z1088:Cdk11b	UTSW	4	155641564	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- CCTGGAATGAACTTGCCCCAGAAG -3'
(R):5'- GGCACGATACCACAGGGTTACAAC -3'

Sequencing Primer
(F):5'- AACTTGCCCCAGAAGTCTAGTTG -3'
(R):5'- TTACAACAACTGGAGTGTAGGC -3'

Posted On

2014-05-14