Incidental Mutation 'R1719:Fbxw21'
ID 191302
Institutional Source Beutler Lab
Gene Symbol Fbxw21
Ensembl Gene ENSMUSG00000047237
Gene Name F-box and WD-40 domain protein 21
Synonyms E330009P21Rik
Accession Numbers
Essential gene? Probably non essential (E-score: 0.062) question?
Stock # R1719 (G1)
Quality Score 225
Status Not validated
Chromosome 9
Chromosomal Location 109139447-109162041 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 109148174 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Threonine to Serine at position 156 (T156S)
Ref Sequence ENSEMBL: ENSMUSP00000143095 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000054925] [ENSMUST00000198076] [ENSMUST00000199540]
AlphaFold Q8BI38
Predicted Effect possibly damaging
Transcript: ENSMUST00000054925
AA Change: T156S

PolyPhen 2 Score 0.854 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000056358
Gene: ENSMUSG00000047237
AA Change: T156S

DomainStartEndE-ValueType
FBOX 5 45 1.46e-6 SMART
SCOP:d1tbga_ 119 249 1e-7 SMART
Blast:WD40 137 176 1e-6 BLAST
Predicted Effect noncoding transcript
Transcript: ENSMUST00000197401
Predicted Effect possibly damaging
Transcript: ENSMUST00000198076
AA Change: T156S

PolyPhen 2 Score 0.854 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000143095
Gene: ENSMUSG00000047237
AA Change: T156S

DomainStartEndE-ValueType
FBOX 5 45 1.46e-6 SMART
SCOP:d1tbga_ 119 249 9e-8 SMART
Blast:WD40 137 176 1e-6 BLAST
Predicted Effect possibly damaging
Transcript: ENSMUST00000199540
AA Change: Y116F

PolyPhen 2 Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000143200
Gene: ENSMUSG00000047237
AA Change: Y116F

DomainStartEndE-ValueType
FBOX 5 45 9e-9 SMART
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.3%
  • 10x: 96.2%
  • 20x: 92.3%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 84 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A1cf A T 19: 31,927,126 (GRCm38) K263M probably damaging Het
Adam26a G A 8: 43,570,036 (GRCm38) T139M possibly damaging Het
Agrp G T 8: 105,566,835 (GRCm38) T106K probably damaging Het
Akap9 C T 5: 3,957,645 (GRCm38) Q238* probably null Het
Ankrd6 A T 4: 32,828,774 (GRCm38) V85E probably damaging Het
Ap2b1 C A 11: 83,324,604 (GRCm38) P125T probably damaging Het
Arhgef7 C A 8: 11,808,713 (GRCm38) probably null Het
Cblc A T 7: 19,790,474 (GRCm38) D280E probably benign Het
Cdk11b T C 4: 155,648,397 (GRCm38) probably benign Het
Cela2a G T 4: 141,817,946 (GRCm38) F239L probably damaging Het
Cfap57 A T 4: 118,606,631 (GRCm38) C342S probably benign Het
Clca4a C A 3: 144,963,755 (GRCm38) W345L probably damaging Het
Col6a5 T C 9: 105,931,293 (GRCm38) D852G unknown Het
Cyp4a32 T C 4: 115,611,308 (GRCm38) V329A possibly damaging Het
Dcaf8 C T 1: 172,175,495 (GRCm38) P287S probably damaging Het
Dmxl1 T A 18: 49,934,637 (GRCm38) D2654E probably damaging Het
Dpep1 A T 8: 123,200,747 (GRCm38) I347F possibly damaging Het
Dtnb T A 12: 3,643,936 (GRCm38) Y56* probably null Het
Duox1 T C 2: 122,338,644 (GRCm38) Y1182H possibly damaging Het
Dusp10 T A 1: 184,037,225 (GRCm38) S129R probably benign Het
Epcam G A 17: 87,642,128 (GRCm38) R173Q probably damaging Het
Ephb3 A G 16: 21,220,650 (GRCm38) E384G probably damaging Het
Exosc10 A T 4: 148,568,503 (GRCm38) D525V probably damaging Het
Fam186a T A 15: 99,942,346 (GRCm38) T2006S possibly damaging Het
Fcrl5 A G 3: 87,457,397 (GRCm38) E568G probably damaging Het
Fmn2 C T 1: 174,608,458 (GRCm38) probably benign Het
Fut9 A G 4: 25,619,744 (GRCm38) F357L possibly damaging Het
Garre1 A T 7: 34,248,206 (GRCm38) M459K probably damaging Het
Gas2l1 G A 11: 5,064,266 (GRCm38) H65Y probably damaging Het
Gjd2 A T 2: 114,013,133 (GRCm38) M1K probably null Het
Hdgfl3 T C 7: 81,899,684 (GRCm38) Y149C probably damaging Het
Hmcn2 T C 2: 31,354,721 (GRCm38) V730A probably damaging Het
Inpp4a A T 1: 37,398,799 (GRCm38) S223C probably damaging Het
Ip6k1 G A 9: 108,040,996 (GRCm38) E77K possibly damaging Het
Knl1 T A 2: 119,071,738 (GRCm38) W1307R probably benign Het
Kpna3 A G 14: 61,387,477 (GRCm38) L139P probably damaging Het
Lama3 T C 18: 12,479,872 (GRCm38) probably null Het
Lin54 G A 5: 100,485,249 (GRCm38) P192L possibly damaging Het
Lpo T A 11: 87,809,192 (GRCm38) probably null Het
Lrrc37 T A 11: 103,617,071 (GRCm38) probably benign Het
Lrrd1 T G 5: 3,850,483 (GRCm38) probably null Het
Nbas T A 12: 13,560,977 (GRCm38) probably null Het
Nemp1 G A 10: 127,696,248 (GRCm38) G341D probably damaging Het
Nrp1 T C 8: 128,425,885 (GRCm38) F192L probably damaging Het
Nufip2 T A 11: 77,693,090 (GRCm38) V610E probably damaging Het
Nup160 T G 2: 90,700,436 (GRCm38) Y479* probably null Het
Oas1d A T 5: 120,919,962 (GRCm38) D323V possibly damaging Het
Or10ak14 A G 4: 118,753,600 (GRCm38) W315R possibly damaging Het
Or14j1 A G 17: 37,835,353 (GRCm38) D118G possibly damaging Het
Or4c3d T C 2: 90,051,784 (GRCm38) Y180C probably damaging Het
Or52e4 C A 7: 105,056,794 (GRCm38) H183N probably damaging Het
Or5ac22 T A 16: 59,314,706 (GRCm38) R234* probably null Het
Or8b3 G A 9: 38,403,254 (GRCm38) V127M possibly damaging Het
Or8c9 A T 9: 38,330,507 (GRCm38) T307S probably benign Het
Pcm1 T A 8: 41,313,359 (GRCm38) M1567K possibly damaging Het
Pdxk A G 10: 78,443,896 (GRCm38) V215A probably benign Het
Phf12 T A 11: 78,023,601 (GRCm38) L74Q probably damaging Het
Plcg1 A G 2: 160,753,743 (GRCm38) E537G probably null Het
Plxna2 C T 1: 194,644,370 (GRCm38) P204L possibly damaging Het
Ppp3cb T G 14: 20,524,063 (GRCm38) M236L probably benign Het
Qrsl1 A T 10: 43,896,030 (GRCm38) S55T probably damaging Het
Rbm5 T C 9: 107,743,913 (GRCm38) probably null Het
Sipa1l2 A G 8: 125,444,535 (GRCm38) S1403P probably damaging Het
Sis C T 3: 72,965,604 (GRCm38) C67Y probably damaging Het
Specc1 A G 11: 62,128,392 (GRCm38) I686V possibly damaging Het
Speg G A 1: 75,417,863 (GRCm38) E1739K probably benign Het
Sprtn C A 8: 124,901,633 (GRCm38) H154Q probably damaging Het
St7l A G 3: 104,870,987 (GRCm38) T147A probably benign Het
Stab1 G A 14: 31,146,028 (GRCm38) Q1630* probably null Het
Stpg2 A G 3: 139,232,199 (GRCm38) D173G probably benign Het
Tcf20 G A 15: 82,852,777 (GRCm38) T1491I probably benign Het
Themis2 A T 4: 132,789,649 (GRCm38) I180N possibly damaging Het
Timd5 T C 11: 46,526,294 (GRCm38) L13P probably damaging Het
Tktl2 G A 8: 66,512,347 (GRCm38) V186M probably damaging Het
Ttn A G 2: 76,807,996 (GRCm38) V13980A probably damaging Het
Ttn C T 2: 76,745,634 (GRCm38) V24972M probably damaging Het
Usp5 A T 6: 124,823,460 (GRCm38) M286K possibly damaging Het
Vmn2r22 G A 6: 123,637,843 (GRCm38) R263C possibly damaging Het
Vmn2r71 T G 7: 85,621,227 (GRCm38) C534G probably damaging Het
Wdr60 T C 12: 116,255,912 (GRCm38) I137V probably benign Het
Wnk2 C T 13: 49,060,726 (GRCm38) S1460N possibly damaging Het
Zfp445 G A 9: 122,852,642 (GRCm38) P745S probably damaging Het
Zfp957 C T 14: 79,213,996 (GRCm38) G121D probably damaging Het
Zscan4f A G 7: 11,401,327 (GRCm38) E220G possibly damaging Het
Other mutations in Fbxw21
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00679:Fbxw21 APN 9 109,161,964 (GRCm38) missense probably damaging 1.00
IGL00901:Fbxw21 APN 9 109,156,399 (GRCm38) missense probably benign 0.00
IGL01377:Fbxw21 APN 9 109,146,645 (GRCm38) nonsense probably null
IGL01941:Fbxw21 APN 9 109,148,156 (GRCm38) missense probably benign 0.07
IGL02491:Fbxw21 APN 9 109,143,819 (GRCm38) missense probably benign
IGL03163:Fbxw21 APN 9 109,145,484 (GRCm38) missense probably benign 0.01
IGL03377:Fbxw21 APN 9 109,139,529 (GRCm38) missense probably benign 0.01
R0148:Fbxw21 UTSW 9 109,148,017 (GRCm38) critical splice donor site probably null
R0328:Fbxw21 UTSW 9 109,146,585 (GRCm38) missense possibly damaging 0.52
R0909:Fbxw21 UTSW 9 109,156,408 (GRCm38) missense possibly damaging 0.84
R1506:Fbxw21 UTSW 9 109,148,189 (GRCm38) missense probably damaging 1.00
R1575:Fbxw21 UTSW 9 109,161,916 (GRCm38) missense probably benign 0.00
R1615:Fbxw21 UTSW 9 109,143,726 (GRCm38) missense probably damaging 1.00
R2415:Fbxw21 UTSW 9 109,156,401 (GRCm38) missense possibly damaging 0.71
R2424:Fbxw21 UTSW 9 109,157,519 (GRCm38) nonsense probably null
R2508:Fbxw21 UTSW 9 109,145,485 (GRCm38) missense probably benign 0.31
R2898:Fbxw21 UTSW 9 109,156,336 (GRCm38) missense possibly damaging 0.52
R2964:Fbxw21 UTSW 9 109,145,510 (GRCm38) missense probably benign 0.10
R2965:Fbxw21 UTSW 9 109,145,510 (GRCm38) missense probably benign 0.10
R2966:Fbxw21 UTSW 9 109,145,510 (GRCm38) missense probably benign 0.10
R4809:Fbxw21 UTSW 9 109,143,390 (GRCm38) missense probably damaging 1.00
R4911:Fbxw21 UTSW 9 109,145,663 (GRCm38) missense probably damaging 1.00
R5669:Fbxw21 UTSW 9 109,145,510 (GRCm38) missense probably benign 0.12
R5928:Fbxw21 UTSW 9 109,143,825 (GRCm38) missense possibly damaging 0.55
R6043:Fbxw21 UTSW 9 109,145,539 (GRCm38) missense possibly damaging 0.69
R6277:Fbxw21 UTSW 9 109,145,555 (GRCm38) missense possibly damaging 0.95
R6805:Fbxw21 UTSW 9 109,157,565 (GRCm38) missense probably damaging 1.00
R6944:Fbxw21 UTSW 9 109,157,535 (GRCm38) missense probably damaging 1.00
R7079:Fbxw21 UTSW 9 109,145,510 (GRCm38) missense probably benign 0.10
R7081:Fbxw21 UTSW 9 109,161,922 (GRCm38) missense probably damaging 1.00
R7744:Fbxw21 UTSW 9 109,157,652 (GRCm38) missense possibly damaging 0.81
R7774:Fbxw21 UTSW 9 109,143,840 (GRCm38) missense probably benign 0.00
R7980:Fbxw21 UTSW 9 109,156,571 (GRCm38) splice site probably null
R8043:Fbxw21 UTSW 9 109,146,626 (GRCm38) missense probably benign 0.01
R8260:Fbxw21 UTSW 9 109,146,546 (GRCm38) critical splice donor site probably null
R9142:Fbxw21 UTSW 9 109,156,345 (GRCm38) missense probably damaging 1.00
R9172:Fbxw21 UTSW 9 109,146,696 (GRCm38) missense probably benign 0.00
R9250:Fbxw21 UTSW 9 109,143,778 (GRCm38) missense probably benign 0.31
R9251:Fbxw21 UTSW 9 109,145,619 (GRCm38) missense probably damaging 1.00
R9294:Fbxw21 UTSW 9 109,143,762 (GRCm38) missense probably damaging 0.99
R9303:Fbxw21 UTSW 9 109,157,659 (GRCm38) missense probably benign
R9479:Fbxw21 UTSW 9 109,139,544 (GRCm38) missense probably benign 0.00
R9509:Fbxw21 UTSW 9 109,148,149 (GRCm38) missense possibly damaging 0.90
R9765:Fbxw21 UTSW 9 109,146,557 (GRCm38) missense possibly damaging 0.71
R9773:Fbxw21 UTSW 9 109,148,060 (GRCm38) missense possibly damaging 0.89
R9774:Fbxw21 UTSW 9 109,161,989 (GRCm38) start codon destroyed probably null 0.98
Z1088:Fbxw21 UTSW 9 109,145,537 (GRCm38) missense probably benign
Z1176:Fbxw21 UTSW 9 109,145,537 (GRCm38) missense probably benign
Z1177:Fbxw21 UTSW 9 109,145,537 (GRCm38) missense probably benign
Predicted Primers PCR Primer
(F):5'- TGGGCCATCCTTGGTAATGACAGC -3'
(R):5'- TGTGTGCTTGCCTCAGAGCATAATG -3'

Sequencing Primer
(F):5'- TGACAGCTTTCAGTGACTGACAG -3'
(R):5'- agccatctctccagccc -3'
Posted On 2014-05-14