|Institutional Source||Beutler Lab|
|Gene Name||acyl-CoA synthetase long-chain family member 3|
|Synonyms||2610510B12Rik, Facl3, C85929|
|Essential gene?||Possibly non essential (E-score: 0.480)|
|Stock #||R1697 (G1)|
|Chromosomal Location||78657825-78707743 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||T to C at 78705397 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000121695 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000035779] [ENSMUST00000134566] [ENSMUST00000142704]|
|Coding Region Coverage||
|Validation Efficiency||99% (67/68)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mice exhibit decreased blood percentages of CD4 T cells and B cells, and a decreased IgG1 response to ovalbumin. Male mutant mice exhibit growth retardation, reduced size and reduced total tissue and lean body mass. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Acsl3||
(F):5'- GCTAAGACGATTAGTAAGCGCAGTACG -3'
(R):5'- ATAGACTCACTGGGCCAGGACACA -3'
(F):5'- AACTTTGGTCCTTGACTAAGTTATCC -3'
(R):5'- caggagacaggaaaaataactaagac -3'