Mutagenetix > Incidental Mutation

Incidental Mutation 'R1761:Cubn'

192742

Institutional Source

Beutler Lab

Gene Symbol

Cubn

Ensembl Gene

ENSMUSG00000026726

Gene Name

cubilin (intrinsic factor-cobalamin receptor)

Synonyms

D2Wsu88e

MMRRC Submission

039793-MU

Accession Numbers

Genbank: NM_001081084; MGI: 1931256

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R1761 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

13276338-13491813 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 13489317 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000089009 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091436] [ENSMUST00000091436]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000091436

SMART Domains

Protein: ENSMUSP00000089009
Gene: ENSMUSG00000026726

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
EGF	132	165	2.14e-5	SMART
EGF_CA	167	208	1.95e-8	SMART
EGF	213	258	2.85e-1	SMART
EGF_CA	260	301	2.66e-10	SMART
EGF_CA	302	345	7.07e-6	SMART
EGF	349	393	1.01e-1	SMART
EGF	398	430	3.73e-5	SMART
EGF_CA	432	468	8.63e-10	SMART
CUB	474	586	4.4e-21	SMART
CUB	590	702	3.82e-39	SMART
CUB	708	816	3.66e-18	SMART
CUB	817	928	3.09e-25	SMART
CUB	932	1042	1.29e-36	SMART
CUB	1048	1161	3.46e-37	SMART
CUB	1165	1277	7.24e-40	SMART
CUB	1278	1389	8.33e-31	SMART
CUB	1391	1506	3.08e-43	SMART
CUB	1510	1619	1.9e-34	SMART
CUB	1620	1734	7.24e-40	SMART
CUB	1738	1850	6.02e-37	SMART
CUB	1852	1963	1.57e-26	SMART
CUB	1978	2091	3.46e-28	SMART
CUB	2092	2213	2.88e-34	SMART
CUB	2217	2334	4.13e-35	SMART
CUB	2336	2448	3.1e-39	SMART
CUB	2452	2565	5.37e-34	SMART
CUB	2570	2687	3e-23	SMART
CUB	2689	2801	3.1e-39	SMART
CUB	2805	2919	2.36e-21	SMART
CUB	2920	3035	6.18e-25	SMART
CUB	3037	3150	5.16e-36	SMART
CUB	3157	3274	1.68e-35	SMART
CUB	3278	3393	7.17e-12	SMART
CUB	3395	3507	2.49e-29	SMART
CUB	3511	3623	2.4e-22	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000091436

SMART Domains

Protein: ENSMUSP00000089009
Gene: ENSMUSG00000026726

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
EGF	132	165	2.14e-5	SMART
EGF_CA	167	208	1.95e-8	SMART
EGF	213	258	2.85e-1	SMART
EGF_CA	260	301	2.66e-10	SMART
EGF_CA	302	345	7.07e-6	SMART
EGF	349	393	1.01e-1	SMART
EGF	398	430	3.73e-5	SMART
EGF_CA	432	468	8.63e-10	SMART
CUB	474	586	4.4e-21	SMART
CUB	590	702	3.82e-39	SMART
CUB	708	816	3.66e-18	SMART
CUB	817	928	3.09e-25	SMART
CUB	932	1042	1.29e-36	SMART
CUB	1048	1161	3.46e-37	SMART
CUB	1165	1277	7.24e-40	SMART
CUB	1278	1389	8.33e-31	SMART
CUB	1391	1506	3.08e-43	SMART
CUB	1510	1619	1.9e-34	SMART
CUB	1620	1734	7.24e-40	SMART
CUB	1738	1850	6.02e-37	SMART
CUB	1852	1963	1.57e-26	SMART
CUB	1978	2091	3.46e-28	SMART
CUB	2092	2213	2.88e-34	SMART
CUB	2217	2334	4.13e-35	SMART
CUB	2336	2448	3.1e-39	SMART
CUB	2452	2565	5.37e-34	SMART
CUB	2570	2687	3e-23	SMART
CUB	2689	2801	3.1e-39	SMART
CUB	2805	2919	2.36e-21	SMART
CUB	2920	3035	6.18e-25	SMART
CUB	3037	3150	5.16e-36	SMART
CUB	3157	3274	1.68e-35	SMART
CUB	3278	3393	7.17e-12	SMART
CUB	3395	3507	2.49e-29	SMART
CUB	3511	3623	2.4e-22	SMART

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null mutation display embryonic lethality during organogenesis, yolk sac and allantoic vasculature defects, embryonic and visceral endoderm defects, and lack somites. Heterozygotes display incomplete penetrance of premature death. [provided by MGI curators]

Allele List at MGI

All alleles(4) : Targeted, other(1) Gene trapped(3)

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110017D15Rik	G	A	4: 41,507,223 (GRCm38)	P109L	probably damaging	Het
Abi3bp	C	T	16: 56,668,309 (GRCm38)	H1268Y	possibly damaging	Het
Acan	T	A	7: 79,094,085 (GRCm38)	Y621*	probably null	Het
Aig1	T	C	10: 13,690,584 (GRCm38)	Y152C	probably damaging	Het
Arhgap33	C	T	7: 30,533,063 (GRCm38)		probably null	Het
Bcl6	G	T	16: 23,977,542 (GRCm38)	A45D	probably damaging	Het
Cbx5	T	C	15: 103,213,180 (GRCm38)	D10G	possibly damaging	Het
Ccdc191	A	G	16: 43,943,510 (GRCm38)	I445V	probably benign	Het
Cdk4	T	A	10: 127,064,677 (GRCm38)		probably benign	Het
Chil6	A	G	3: 106,394,338 (GRCm38)	F149L	probably damaging	Het
Cntn5	T	C	9: 10,172,054 (GRCm38)	T42A	probably benign	Het
Cpn2	A	T	16: 30,260,196 (GRCm38)	I229N	probably damaging	Het
Cpne8	A	G	15: 90,648,618 (GRCm38)	V62A	probably damaging	Het
Cr2	A	G	1: 195,155,123 (GRCm38)		probably null	Het
Crnn	A	T	3: 93,148,651 (GRCm38)	H248L	probably benign	Het
Csn1s1	A	G	5: 87,679,035 (GRCm38)	S254G	probably benign	Het
Dnah8	A	G	17: 30,779,916 (GRCm38)	N3525S	probably damaging	Het
Dpp3	A	G	19: 4,921,149 (GRCm38)	L220P	probably benign	Het
Fam110a	T	C	2: 151,970,205 (GRCm38)	E215G	probably benign	Het
Fam20a	A	T	11: 109,677,838 (GRCm38)	N287K	probably damaging	Het
Fat4	T	C	3: 38,887,489 (GRCm38)	V177A	possibly damaging	Het
Fzd8	C	T	18: 9,213,643 (GRCm38)	R242C	probably damaging	Het
Gimap5	A	T	6: 48,753,261 (GRCm38)	Q255L	probably damaging	Het
Gm13083	T	C	4: 143,615,868 (GRCm38)	Y182H	probably benign	Het
Gm4787	A	G	12: 81,377,176 (GRCm38)	L736S	probably benign	Het
Gmeb1	G	A	4: 132,234,887 (GRCm38)	Q154*	probably null	Het
Gpr156	T	C	16: 37,987,567 (GRCm38)	L192P	probably damaging	Het
Gpr179	A	C	11: 97,335,106 (GRCm38)	S2074R	probably benign	Het
Hddc2	G	T	10: 31,326,139 (GRCm38)	D161Y	probably damaging	Het
Hlcs	T	C	16: 94,268,007 (GRCm38)	D265G	probably benign	Het
Hspg2	A	T	4: 137,514,673 (GRCm38)	I573F	possibly damaging	Het
Il1b	T	C	2: 129,365,181 (GRCm38)	K220E	probably damaging	Het
Il5	T	C	11: 53,723,730 (GRCm38)	I66T	probably damaging	Het
Irf6	G	A	1: 193,169,301 (GRCm38)	R400H	probably damaging	Het
Klra1	A	T	6: 130,372,873 (GRCm38)	Y201N	probably damaging	Het
Lmf2	A	G	15: 89,352,713 (GRCm38)	V442A	possibly damaging	Het
Mcm2	T	C	6: 88,889,788 (GRCm38)	I412M	possibly damaging	Het
Mlkl	T	A	8: 111,333,723 (GRCm38)	L18F	possibly damaging	Het
Mug2	C	A	6: 122,074,705 (GRCm38)	H949N	probably benign	Het
Nf1	T	C	11: 79,384,265 (GRCm38)	F51L	probably damaging	Het
Olfr1049	T	G	2: 86,255,039 (GRCm38)	Y218S	probably damaging	Het
Olfr592	T	G	7: 103,187,118 (GRCm38)	C172W	probably damaging	Het
Olfr77	A	T	9: 19,921,149 (GRCm38)	*313C	probably null	Het
P3h2	T	C	16: 25,985,050 (GRCm38)	E322G	probably damaging	Het
Psmg2	CTTCAGTT	CTTCAGTTCAGTT	18: 67,646,025 (GRCm38)		probably null	Het
Ptdss1	T	C	13: 66,956,412 (GRCm38)	V116A	possibly damaging	Het
Ranbp2	T	C	10: 58,485,741 (GRCm38)	V2620A	probably benign	Het
Robo2	A	G	16: 74,035,024 (GRCm38)	V256A	probably damaging	Het
Scg3	T	A	9: 75,676,758 (GRCm38)	I154F	probably damaging	Het
Scgn	A	T	13: 23,959,706 (GRCm38)	F225Y	probably damaging	Het
Sec61b	T	C	4: 47,480,137 (GRCm38)	C58R	possibly damaging	Het
Slc25a46	G	T	18: 31,607,262 (GRCm38)	Q96K	possibly damaging	Het
Sptb	A	G	12: 76,612,608 (GRCm38)	F1173L	probably damaging	Het
Srcap	T	A	7: 127,534,845 (GRCm38)	C893S	probably damaging	Het
Tet3	T	C	6: 83,403,659 (GRCm38)	E509G	probably damaging	Het
Timm10b	C	T	7: 105,683,708 (GRCm38)	R897*	probably null	Het
Tln2	C	T	9: 67,286,514 (GRCm38)	A1773T	probably benign	Het
Tom1	T	C	8: 75,051,551 (GRCm38)	V87A	probably benign	Het
Tti1	T	C	2: 158,007,697 (GRCm38)	I541V	probably benign	Het
Ttn	A	T	2: 76,811,243 (GRCm38)	L5176Q	possibly damaging	Het
Tubb1	A	G	2: 174,456,896 (GRCm38)	S124G	probably benign	Het
Vgll3	A	T	16: 65,839,728 (GRCm38)	D310V	probably damaging	Het
Vmac	A	G	17: 56,715,788 (GRCm38)	L74P	probably damaging	Het
Zbtb1	A	T	12: 76,385,821 (GRCm38)	K194*	probably null	Het
Zfp429	A	G	13: 67,396,076 (GRCm38)	M76T	probably benign	Het
Zfp808	T	A	13: 62,171,646 (GRCm38)	C230S	possibly damaging	Het
Zfp980	A	G	4: 145,702,042 (GRCm38)	Y447C	probably damaging	Het
Zfp985	A	G	4: 147,584,045 (GRCm38)	T457A	probably benign	Het

Other mutations in Cubn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Cubn	APN	2	13,491,820 (GRCm38)	unclassified	probably benign
IGL00228:Cubn	APN	2	13,456,697 (GRCm38)	missense	probably damaging	1.00
IGL00231:Cubn	APN	2	13,381,849 (GRCm38)	missense	possibly damaging	0.89
IGL00327:Cubn	APN	2	13,427,056 (GRCm38)	missense	possibly damaging	0.73
IGL00470:Cubn	APN	2	13,278,418 (GRCm38)	missense	probably benign	0.00
IGL00519:Cubn	APN	2	13,282,919 (GRCm38)	missense	probably benign	0.00
IGL00562:Cubn	APN	2	13,294,230 (GRCm38)	missense	probably benign	0.01
IGL00678:Cubn	APN	2	13,467,710 (GRCm38)	missense	possibly damaging	0.47
IGL00834:Cubn	APN	2	13,381,927 (GRCm38)	missense	probably damaging	1.00
IGL00946:Cubn	APN	2	13,456,623 (GRCm38)	missense	probably damaging	0.98
IGL00971:Cubn	APN	2	13,278,408 (GRCm38)	missense	possibly damaging	0.77
IGL01124:Cubn	APN	2	13,478,093 (GRCm38)	missense	possibly damaging	0.62
IGL01287:Cubn	APN	2	13,310,566 (GRCm38)	missense	probably damaging	1.00
IGL01410:Cubn	APN	2	13,465,908 (GRCm38)	missense	probably benign	0.31
IGL01418:Cubn	APN	2	13,284,041 (GRCm38)	missense	probably benign	0.01
IGL01450:Cubn	APN	2	13,350,862 (GRCm38)	splice site	probably benign
IGL01534:Cubn	APN	2	13,465,933 (GRCm38)	nonsense	probably null
IGL01584:Cubn	APN	2	13,308,661 (GRCm38)	splice site	probably benign
IGL01595:Cubn	APN	2	13,325,216 (GRCm38)	missense	probably benign	0.05
IGL01625:Cubn	APN	2	13,306,274 (GRCm38)	missense	possibly damaging	0.76
IGL01732:Cubn	APN	2	13,489,936 (GRCm38)	nonsense	probably null
IGL01972:Cubn	APN	2	13,446,072 (GRCm38)	missense	possibly damaging	0.90
IGL02027:Cubn	APN	2	13,287,594 (GRCm38)	missense	probably damaging	1.00
IGL02033:Cubn	APN	2	13,339,846 (GRCm38)	missense	probably damaging	0.98
IGL02124:Cubn	APN	2	13,381,837 (GRCm38)	missense	probably damaging	0.99
IGL02335:Cubn	APN	2	13,427,834 (GRCm38)	splice site	probably null
IGL02491:Cubn	APN	2	13,321,228 (GRCm38)	missense	probably damaging	1.00
IGL02686:Cubn	APN	2	13,325,226 (GRCm38)	missense	possibly damaging	0.92
IGL02707:Cubn	APN	2	13,446,032 (GRCm38)	missense	probably damaging	0.99
IGL02746:Cubn	APN	2	13,445,040 (GRCm38)	missense	probably damaging	1.00
IGL02873:Cubn	APN	2	13,294,370 (GRCm38)	missense	probably benign	0.07
IGL02897:Cubn	APN	2	13,318,312 (GRCm38)	missense	possibly damaging	0.55
IGL03078:Cubn	APN	2	13,287,094 (GRCm38)	missense	possibly damaging	0.87
IGL03245:Cubn	APN	2	13,355,689 (GRCm38)	missense	probably benign	0.09
IGL03289:Cubn	APN	2	13,426,967 (GRCm38)	missense	probably benign	0.00
IGL03335:Cubn	APN	2	13,360,329 (GRCm38)	missense	probably damaging	1.00
IGL03355:Cubn	APN	2	13,478,057 (GRCm38)	splice site	probably null
mellow	UTSW	2	13,478,078 (GRCm38)	missense	probably damaging	1.00
PIT4354001:Cubn	UTSW	2	13,468,852 (GRCm38)	nonsense	probably null
PIT4495001:Cubn	UTSW	2	13,491,750 (GRCm38)	missense	probably benign	0.00
R0145:Cubn	UTSW	2	13,306,432 (GRCm38)	missense	probably damaging	1.00
R0220:Cubn	UTSW	2	13,356,709 (GRCm38)	missense	probably damaging	1.00
R0254:Cubn	UTSW	2	13,476,035 (GRCm38)	critical splice donor site	probably null
R0254:Cubn	UTSW	2	13,440,514 (GRCm38)	missense	possibly damaging	0.84
R0254:Cubn	UTSW	2	13,424,694 (GRCm38)	missense	probably benign	0.01
R0360:Cubn	UTSW	2	13,310,507 (GRCm38)	splice site	probably benign
R0364:Cubn	UTSW	2	13,310,507 (GRCm38)	splice site	probably benign
R0383:Cubn	UTSW	2	13,430,959 (GRCm38)	missense	probably damaging	1.00
R0419:Cubn	UTSW	2	13,469,764 (GRCm38)	missense	possibly damaging	0.87
R0419:Cubn	UTSW	2	13,469,763 (GRCm38)	missense	possibly damaging	0.77
R0498:Cubn	UTSW	2	13,444,267 (GRCm38)	missense	probably damaging	0.99
R0560:Cubn	UTSW	2	13,428,680 (GRCm38)	missense	probably damaging	1.00
R0615:Cubn	UTSW	2	13,360,252 (GRCm38)	splice site	probably null
R0735:Cubn	UTSW	2	13,491,689 (GRCm38)	splice site	probably benign
R0780:Cubn	UTSW	2	13,456,613 (GRCm38)	missense	probably damaging	1.00
R0899:Cubn	UTSW	2	13,362,328 (GRCm38)	missense	possibly damaging	0.54
R1118:Cubn	UTSW	2	13,336,242 (GRCm38)	missense	possibly damaging	0.78
R1182:Cubn	UTSW	2	13,445,000 (GRCm38)	missense	probably damaging	0.98
R1439:Cubn	UTSW	2	13,287,568 (GRCm38)	missense	probably damaging	0.96
R1450:Cubn	UTSW	2	13,360,319 (GRCm38)	missense	probably damaging	1.00
R1464:Cubn	UTSW	2	13,325,288 (GRCm38)	missense	possibly damaging	0.87
R1464:Cubn	UTSW	2	13,325,288 (GRCm38)	missense	possibly damaging	0.87
R1476:Cubn	UTSW	2	13,476,120 (GRCm38)	missense	probably benign	0.04
R1508:Cubn	UTSW	2	13,427,105 (GRCm38)	missense	probably benign	0.25
R1532:Cubn	UTSW	2	13,287,661 (GRCm38)	missense	probably damaging	1.00
R1562:Cubn	UTSW	2	13,427,967 (GRCm38)	missense	probably damaging	1.00
R1598:Cubn	UTSW	2	13,469,789 (GRCm38)	missense	probably benign	0.00
R1862:Cubn	UTSW	2	13,308,561 (GRCm38)	missense	probably damaging	1.00
R1874:Cubn	UTSW	2	13,323,002 (GRCm38)	missense	probably damaging	1.00
R1923:Cubn	UTSW	2	13,310,526 (GRCm38)	missense	probably damaging	1.00
R1944:Cubn	UTSW	2	13,278,538 (GRCm38)	missense	probably benign	0.01
R1960:Cubn	UTSW	2	13,340,017 (GRCm38)	splice site	probably null
R2021:Cubn	UTSW	2	13,308,549 (GRCm38)	missense	probably benign	0.09
R2137:Cubn	UTSW	2	13,336,167 (GRCm38)	missense	probably benign	0.01
R2138:Cubn	UTSW	2	13,444,378 (GRCm38)	missense	probably damaging	0.99
R2139:Cubn	UTSW	2	13,336,167 (GRCm38)	missense	probably benign	0.01
R2179:Cubn	UTSW	2	13,318,242 (GRCm38)	missense	possibly damaging	0.85
R2328:Cubn	UTSW	2	13,404,080 (GRCm38)	nonsense	probably null
R2369:Cubn	UTSW	2	13,491,217 (GRCm38)	missense	probably damaging	1.00
R2428:Cubn	UTSW	2	13,476,150 (GRCm38)	missense	probably damaging	1.00
R2435:Cubn	UTSW	2	13,318,272 (GRCm38)	missense	probably damaging	1.00
R2567:Cubn	UTSW	2	13,278,356 (GRCm38)	splice site	probably null
R2850:Cubn	UTSW	2	13,322,953 (GRCm38)	missense	probably damaging	1.00
R2853:Cubn	UTSW	2	13,430,834 (GRCm38)	missense	probably benign	0.00
R2893:Cubn	UTSW	2	13,358,139 (GRCm38)	missense	possibly damaging	0.61
R3107:Cubn	UTSW	2	13,362,347 (GRCm38)	missense	possibly damaging	0.73
R3109:Cubn	UTSW	2	13,362,347 (GRCm38)	missense	possibly damaging	0.73
R3119:Cubn	UTSW	2	13,358,162 (GRCm38)	missense	possibly damaging	0.90
R3405:Cubn	UTSW	2	13,333,508 (GRCm38)	missense	probably benign	0.00
R3703:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3704:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3705:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3764:Cubn	UTSW	2	13,331,585 (GRCm38)	missense	possibly damaging	0.79
R3792:Cubn	UTSW	2	13,427,914 (GRCm38)	missense	probably damaging	1.00
R3802:Cubn	UTSW	2	13,360,353 (GRCm38)	missense	probably benign	0.01
R3813:Cubn	UTSW	2	13,294,325 (GRCm38)	missense	probably damaging	1.00
R3845:Cubn	UTSW	2	13,283,008 (GRCm38)	missense	probably damaging	1.00
R3846:Cubn	UTSW	2	13,283,008 (GRCm38)	missense	probably damaging	1.00
R3900:Cubn	UTSW	2	13,286,980 (GRCm38)	critical splice donor site	probably null
R3921:Cubn	UTSW	2	13,326,677 (GRCm38)	missense	probably damaging	1.00
R4075:Cubn	UTSW	2	13,313,999 (GRCm38)	missense	possibly damaging	0.58
R4082:Cubn	UTSW	2	13,428,563 (GRCm38)	intron	probably benign
R4405:Cubn	UTSW	2	13,466,030 (GRCm38)	missense	probably damaging	1.00
R4615:Cubn	UTSW	2	13,428,749 (GRCm38)	missense	probably damaging	1.00
R4629:Cubn	UTSW	2	13,313,979 (GRCm38)	splice site	probably null
R4770:Cubn	UTSW	2	13,314,767 (GRCm38)	missense	possibly damaging	0.92
R4799:Cubn	UTSW	2	13,287,024 (GRCm38)	missense	possibly damaging	0.94
R4799:Cubn	UTSW	2	13,351,058 (GRCm38)	missense	probably damaging	1.00
R4812:Cubn	UTSW	2	13,459,076 (GRCm38)	missense	probably damaging	1.00
R4825:Cubn	UTSW	2	13,325,225 (GRCm38)	missense	probably damaging	1.00
R4934:Cubn	UTSW	2	13,489,910 (GRCm38)	missense	probably benign	0.06
R4967:Cubn	UTSW	2	13,348,045 (GRCm38)	missense	probably benign	0.01
R5187:Cubn	UTSW	2	13,287,568 (GRCm38)	missense	probably damaging	0.96
R5232:Cubn	UTSW	2	13,478,202 (GRCm38)	nonsense	probably null
R5305:Cubn	UTSW	2	13,388,939 (GRCm38)	missense	probably damaging	1.00
R5506:Cubn	UTSW	2	13,491,695 (GRCm38)	splice site	probably null
R5530:Cubn	UTSW	2	13,308,523 (GRCm38)	missense	probably damaging	1.00
R5531:Cubn	UTSW	2	13,350,932 (GRCm38)	missense	probably benign	0.00
R5737:Cubn	UTSW	2	13,388,891 (GRCm38)	missense	probably damaging	1.00
R5886:Cubn	UTSW	2	13,320,023 (GRCm38)	splice site	probably benign
R5923:Cubn	UTSW	2	13,486,078 (GRCm38)	missense	possibly damaging	0.73
R6032:Cubn	UTSW	2	13,325,184 (GRCm38)	missense	probably benign	0.12
R6032:Cubn	UTSW	2	13,325,184 (GRCm38)	missense	probably benign	0.12
R6084:Cubn	UTSW	2	13,430,897 (GRCm38)	missense	probably damaging	1.00
R6087:Cubn	UTSW	2	13,427,847 (GRCm38)	missense	probably damaging	1.00
R6133:Cubn	UTSW	2	13,308,618 (GRCm38)	missense	probably benign	0.29
R6181:Cubn	UTSW	2	13,349,876 (GRCm38)	missense	probably benign	0.31
R6301:Cubn	UTSW	2	13,478,078 (GRCm38)	missense	probably damaging	1.00
R6320:Cubn	UTSW	2	13,280,195 (GRCm38)	missense	probably damaging	1.00
R6368:Cubn	UTSW	2	13,476,123 (GRCm38)	missense	probably damaging	0.98
R6368:Cubn	UTSW	2	13,430,995 (GRCm38)	missense	probably damaging	0.96
R6383:Cubn	UTSW	2	13,427,835 (GRCm38)	critical splice donor site	probably null
R6393:Cubn	UTSW	2	13,355,680 (GRCm38)	missense	probably benign	0.08
R6408:Cubn	UTSW	2	13,294,203 (GRCm38)	missense	probably damaging	1.00
R6470:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R6532:Cubn	UTSW	2	13,459,002 (GRCm38)	missense	probably benign	0.01
R6599:Cubn	UTSW	2	13,310,673 (GRCm38)	missense	possibly damaging	0.95
R6629:Cubn	UTSW	2	13,430,872 (GRCm38)	missense	probably damaging	1.00
R6641:Cubn	UTSW	2	13,476,064 (GRCm38)	missense	probably damaging	1.00
R6800:Cubn	UTSW	2	13,321,255 (GRCm38)	missense	probably damaging	1.00
R6823:Cubn	UTSW	2	13,445,029 (GRCm38)	missense	probably benign	0.21
R6847:Cubn	UTSW	2	13,444,253 (GRCm38)	critical splice donor site	probably null
R6885:Cubn	UTSW	2	13,318,278 (GRCm38)	missense	probably damaging	1.00
R6962:Cubn	UTSW	2	13,348,029 (GRCm38)	missense	probably benign	0.03
R6973:Cubn	UTSW	2	13,381,837 (GRCm38)	missense	possibly damaging	0.61
R6975:Cubn	UTSW	2	13,486,789 (GRCm38)	missense	probably damaging	0.99
R7076:Cubn	UTSW	2	13,306,281 (GRCm38)	missense	probably benign	0.10
R7076:Cubn	UTSW	2	13,306,280 (GRCm38)	missense	probably benign	0.00
R7086:Cubn	UTSW	2	13,319,858 (GRCm38)	missense	probably damaging	0.98
R7162:Cubn	UTSW	2	13,342,498 (GRCm38)	missense	probably damaging	0.96
R7203:Cubn	UTSW	2	13,351,003 (GRCm38)	missense	probably benign	0.01
R7292:Cubn	UTSW	2	13,424,739 (GRCm38)	missense	probably damaging	0.99
R7307:Cubn	UTSW	2	13,340,332 (GRCm38)	missense	probably damaging	0.99
R7329:Cubn	UTSW	2	13,468,771 (GRCm38)	missense	probably damaging	0.99
R7395:Cubn	UTSW	2	13,287,064 (GRCm38)	missense	probably damaging	1.00
R7417:Cubn	UTSW	2	13,426,967 (GRCm38)	missense	probably benign	0.00
R7429:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R7430:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R7443:Cubn	UTSW	2	13,455,509 (GRCm38)	missense	probably damaging	1.00
R7699:Cubn	UTSW	2	13,489,917 (GRCm38)	missense	possibly damaging	0.74
R7699:Cubn	UTSW	2	13,348,178 (GRCm38)	missense	probably benign
R7700:Cubn	UTSW	2	13,489,917 (GRCm38)	missense	possibly damaging	0.74
R7700:Cubn	UTSW	2	13,348,178 (GRCm38)	missense	probably benign
R7751:Cubn	UTSW	2	13,360,365 (GRCm38)	missense	probably damaging	1.00
R7755:Cubn	UTSW	2	13,280,078 (GRCm38)	missense	probably benign	0.11
R7759:Cubn	UTSW	2	13,348,150 (GRCm38)	missense	probably damaging	1.00
R7903:Cubn	UTSW	2	13,468,869 (GRCm38)	missense	probably damaging	0.97
R7921:Cubn	UTSW	2	13,424,727 (GRCm38)	missense	probably benign	0.22
R7988:Cubn	UTSW	2	13,332,355 (GRCm38)	missense	probably benign	0.43
R8010:Cubn	UTSW	2	13,336,086 (GRCm38)	critical splice donor site	probably null
R8020:Cubn	UTSW	2	13,479,178 (GRCm38)	missense	probably benign	0.01
R8120:Cubn	UTSW	2	13,331,660 (GRCm38)	missense	probably damaging	1.00
R8133:Cubn	UTSW	2	13,388,848 (GRCm38)	missense	probably damaging	1.00
R8185:Cubn	UTSW	2	13,294,318 (GRCm38)	missense	probably benign	0.11
R8224:Cubn	UTSW	2	13,349,877 (GRCm38)	missense	probably benign	0.16
R8289:Cubn	UTSW	2	13,486,802 (GRCm38)	missense	probably benign	0.10
R8326:Cubn	UTSW	2	13,306,463 (GRCm38)	missense	probably benign	0.01
R8331:Cubn	UTSW	2	13,340,242 (GRCm38)	missense	probably damaging	1.00
R8338:Cubn	UTSW	2	13,430,847 (GRCm38)	missense	probably benign	0.08
R8341:Cubn	UTSW	2	13,428,724 (GRCm38)	missense	probably damaging	1.00
R8358:Cubn	UTSW	2	13,325,160 (GRCm38)	missense	probably benign	0.17
R8427:Cubn	UTSW	2	13,428,756 (GRCm38)	missense	probably benign	0.00
R8432:Cubn	UTSW	2	13,381,799 (GRCm38)	missense	probably benign	0.00
R8441:Cubn	UTSW	2	13,427,847 (GRCm38)	missense	probably damaging	1.00
R8442:Cubn	UTSW	2	13,314,044 (GRCm38)	missense	probably damaging	1.00
R8520:Cubn	UTSW	2	13,308,520 (GRCm38)	critical splice donor site	probably null
R8699:Cubn	UTSW	2	13,383,959 (GRCm38)	missense	probably damaging	1.00
R8753:Cubn	UTSW	2	13,308,566 (GRCm38)	nonsense	probably null
R8874:Cubn	UTSW	2	13,360,346 (GRCm38)	missense	possibly damaging	0.63
R9056:Cubn	UTSW	2	13,456,655 (GRCm38)	missense	probably damaging	1.00
R9079:Cubn	UTSW	2	13,287,103 (GRCm38)	missense	probably benign	0.02
R9143:Cubn	UTSW	2	13,332,465 (GRCm38)	splice site	probably benign
R9261:Cubn	UTSW	2	13,278,451 (GRCm38)	missense	probably damaging	1.00
R9338:Cubn	UTSW	2	13,381,892 (GRCm38)	missense	probably damaging	1.00
R9342:Cubn	UTSW	2	13,458,956 (GRCm38)	missense	probably damaging	0.99
R9603:Cubn	UTSW	2	13,287,699 (GRCm38)	missense	probably damaging	1.00
R9614:Cubn	UTSW	2	13,478,134 (GRCm38)	missense	probably benign	0.00
R9615:Cubn	UTSW	2	13,321,180 (GRCm38)	missense	possibly damaging	0.88
R9616:Cubn	UTSW	2	13,314,718 (GRCm38)	missense	probably benign	0.04
R9774:Cubn	UTSW	2	13,428,719 (GRCm38)	missense	probably benign
X0018:Cubn	UTSW	2	13,458,986 (GRCm38)	missense	probably damaging	1.00
X0022:Cubn	UTSW	2	13,476,076 (GRCm38)	missense	probably damaging	1.00
X0026:Cubn	UTSW	2	13,342,581 (GRCm38)	missense	probably damaging	1.00
X0063:Cubn	UTSW	2	13,322,962 (GRCm38)	missense	probably damaging	1.00
YA93:Cubn	UTSW	2	13,383,992 (GRCm38)	missense	probably benign	0.21
Z1088:Cubn	UTSW	2	13,294,229 (GRCm38)	missense	probably benign	0.43
Z1176:Cubn	UTSW	2	13,381,825 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGGCATACTGCCCTGGGTTTTC -3'
(R):5'- AGTCTTGATAGCATCTTCCTTGCGAAC -3'

Sequencing Primer
(F):5'- CAAGTCCTTGGTATTGATTGACC -3'
(R):5'- AGCTTATCAGTGGGCTGAAC -3'

Posted On

2014-05-23