Mutagenetix > Incidental Mutation

Incidental Mutation 'R1761:Tln2'

192781

Institutional Source

Beutler Lab

Gene Symbol

Tln2

Ensembl Gene

ENSMUSG00000052698

Gene Name

talin 2

Synonyms

MMRRC Submission

039793-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.216) question?

Stock #

R1761 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

67217087-67559703 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 67286514 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 1773 (A1773T)

Ref Sequence

ENSEMBL: ENSMUSP00000148901 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039662] [ENSMUST00000040025] [ENSMUST00000215267] [ENSMUST00000215784] [ENSMUST00000217550]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000039662
AA Change: A1771T

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000035272
Gene: ENSMUSG00000052698
AA Change: A1771T

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	3.4e-59	PFAM
Pfam:I_LWEQ	661	765	1.9e-10	PFAM
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	2.6e-67	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2384	2531	2.5e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000040025
AA Change: A1771T

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000039633
Gene: ENSMUSG00000052698
AA Change: A1771T

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	8.5e-78	PFAM
low complexity region	674	693	N/A	INTRINSIC
internal_repeat_2	703	763	2.05e-7	PROSPERO
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	9.9e-72	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2383	2533	4.3e-51	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000214859
AA Change: A225T

Predicted Effect

probably benign
Transcript: ENSMUST00000215267
AA Change: A681T

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

Predicted Effect

probably benign
Transcript: ENSMUST00000215784
AA Change: A1773T

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

Predicted Effect

probably benign
Transcript: ENSMUST00000217550
AA Change: A685T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Meta Mutation Damage Score

0.0737

Coding Region Coverage

1x: 97.5%
3x: 96.9%
10x: 95.4%
20x: 92.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal muscle morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110017D15Rik	G	A	4: 41,507,223 (GRCm38)	P109L	probably damaging	Het
Abi3bp	C	T	16: 56,668,309 (GRCm38)	H1268Y	possibly damaging	Het
Acan	T	A	7: 79,094,085 (GRCm38)	Y621*	probably null	Het
Aig1	T	C	10: 13,690,584 (GRCm38)	Y152C	probably damaging	Het
Arhgap33	C	T	7: 30,533,063 (GRCm38)		probably null	Het
Bcl6	G	T	16: 23,977,542 (GRCm38)	A45D	probably damaging	Het
Cbx5	T	C	15: 103,213,180 (GRCm38)	D10G	possibly damaging	Het
Ccdc191	A	G	16: 43,943,510 (GRCm38)	I445V	probably benign	Het
Cdk4	T	A	10: 127,064,677 (GRCm38)		probably benign	Het
Chil6	A	G	3: 106,394,338 (GRCm38)	F149L	probably damaging	Het
Cntn5	T	C	9: 10,172,054 (GRCm38)	T42A	probably benign	Het
Cpn2	A	T	16: 30,260,196 (GRCm38)	I229N	probably damaging	Het
Cpne8	A	G	15: 90,648,618 (GRCm38)	V62A	probably damaging	Het
Cr2	A	G	1: 195,155,123 (GRCm38)		probably null	Het
Crnn	A	T	3: 93,148,651 (GRCm38)	H248L	probably benign	Het
Csn1s1	A	G	5: 87,679,035 (GRCm38)	S254G	probably benign	Het
Cubn	A	G	2: 13,489,317 (GRCm38)		probably null	Het
Dnah8	A	G	17: 30,779,916 (GRCm38)	N3525S	probably damaging	Het
Dpp3	A	G	19: 4,921,149 (GRCm38)	L220P	probably benign	Het
Fam110a	T	C	2: 151,970,205 (GRCm38)	E215G	probably benign	Het
Fam20a	A	T	11: 109,677,838 (GRCm38)	N287K	probably damaging	Het
Fat4	T	C	3: 38,887,489 (GRCm38)	V177A	possibly damaging	Het
Fzd8	C	T	18: 9,213,643 (GRCm38)	R242C	probably damaging	Het
Gimap5	A	T	6: 48,753,261 (GRCm38)	Q255L	probably damaging	Het
Gm13083	T	C	4: 143,615,868 (GRCm38)	Y182H	probably benign	Het
Gm4787	A	G	12: 81,377,176 (GRCm38)	L736S	probably benign	Het
Gmeb1	G	A	4: 132,234,887 (GRCm38)	Q154*	probably null	Het
Gpr156	T	C	16: 37,987,567 (GRCm38)	L192P	probably damaging	Het
Gpr179	A	C	11: 97,335,106 (GRCm38)	S2074R	probably benign	Het
Hddc2	G	T	10: 31,326,139 (GRCm38)	D161Y	probably damaging	Het
Hlcs	T	C	16: 94,268,007 (GRCm38)	D265G	probably benign	Het
Hspg2	A	T	4: 137,514,673 (GRCm38)	I573F	possibly damaging	Het
Il1b	T	C	2: 129,365,181 (GRCm38)	K220E	probably damaging	Het
Il5	T	C	11: 53,723,730 (GRCm38)	I66T	probably damaging	Het
Irf6	G	A	1: 193,169,301 (GRCm38)	R400H	probably damaging	Het
Klra1	A	T	6: 130,372,873 (GRCm38)	Y201N	probably damaging	Het
Lmf2	A	G	15: 89,352,713 (GRCm38)	V442A	possibly damaging	Het
Mcm2	T	C	6: 88,889,788 (GRCm38)	I412M	possibly damaging	Het
Mlkl	T	A	8: 111,333,723 (GRCm38)	L18F	possibly damaging	Het
Mug2	C	A	6: 122,074,705 (GRCm38)	H949N	probably benign	Het
Nf1	T	C	11: 79,384,265 (GRCm38)	F51L	probably damaging	Het
Olfr1049	T	G	2: 86,255,039 (GRCm38)	Y218S	probably damaging	Het
Olfr592	T	G	7: 103,187,118 (GRCm38)	C172W	probably damaging	Het
Olfr77	A	T	9: 19,921,149 (GRCm38)	*313C	probably null	Het
P3h2	T	C	16: 25,985,050 (GRCm38)	E322G	probably damaging	Het
Psmg2	CTTCAGTT	CTTCAGTTCAGTT	18: 67,646,025 (GRCm38)		probably null	Het
Ptdss1	T	C	13: 66,956,412 (GRCm38)	V116A	possibly damaging	Het
Ranbp2	T	C	10: 58,485,741 (GRCm38)	V2620A	probably benign	Het
Robo2	A	G	16: 74,035,024 (GRCm38)	V256A	probably damaging	Het
Scg3	T	A	9: 75,676,758 (GRCm38)	I154F	probably damaging	Het
Scgn	A	T	13: 23,959,706 (GRCm38)	F225Y	probably damaging	Het
Sec61b	T	C	4: 47,480,137 (GRCm38)	C58R	possibly damaging	Het
Slc25a46	G	T	18: 31,607,262 (GRCm38)	Q96K	possibly damaging	Het
Sptb	A	G	12: 76,612,608 (GRCm38)	F1173L	probably damaging	Het
Srcap	T	A	7: 127,534,845 (GRCm38)	C893S	probably damaging	Het
Tet3	T	C	6: 83,403,659 (GRCm38)	E509G	probably damaging	Het
Timm10b	C	T	7: 105,683,708 (GRCm38)	R897*	probably null	Het
Tom1	T	C	8: 75,051,551 (GRCm38)	V87A	probably benign	Het
Tti1	T	C	2: 158,007,697 (GRCm38)	I541V	probably benign	Het
Ttn	A	T	2: 76,811,243 (GRCm38)	L5176Q	possibly damaging	Het
Tubb1	A	G	2: 174,456,896 (GRCm38)	S124G	probably benign	Het
Vgll3	A	T	16: 65,839,728 (GRCm38)	D310V	probably damaging	Het
Vmac	A	G	17: 56,715,788 (GRCm38)	L74P	probably damaging	Het
Zbtb1	A	T	12: 76,385,821 (GRCm38)	K194*	probably null	Het
Zfp429	A	G	13: 67,396,076 (GRCm38)	M76T	probably benign	Het
Zfp808	T	A	13: 62,171,646 (GRCm38)	C230S	possibly damaging	Het
Zfp980	A	G	4: 145,702,042 (GRCm38)	Y447C	probably damaging	Het
Zfp985	A	G	4: 147,584,045 (GRCm38)	T457A	probably benign	Het

Other mutations in Tln2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00468:Tln2	APN	9	67,344,187 (GRCm38)	missense	possibly damaging	0.59
IGL01110:Tln2	APN	9	67,250,582 (GRCm38)	nonsense	probably null
IGL01112:Tln2	APN	9	67,311,811 (GRCm38)	missense	probably damaging	1.00
IGL01307:Tln2	APN	9	67,395,467 (GRCm38)	missense	probably benign	0.25
IGL01374:Tln2	APN	9	67,261,923 (GRCm38)	missense	probably damaging	1.00
IGL01625:Tln2	APN	9	67,370,623 (GRCm38)	missense	probably damaging	1.00
IGL01865:Tln2	APN	9	67,250,614 (GRCm38)	nonsense	probably null
IGL01999:Tln2	APN	9	67,392,505 (GRCm38)	missense	possibly damaging	0.81
IGL02002:Tln2	APN	9	67,356,698 (GRCm38)	missense	probably damaging	0.98
IGL02005:Tln2	APN	9	67,392,505 (GRCm38)	missense	possibly damaging	0.81
IGL02015:Tln2	APN	9	67,361,439 (GRCm38)	splice site	probably benign
IGL02368:Tln2	APN	9	67,240,810 (GRCm38)	splice site	probably benign
IGL02444:Tln2	APN	9	67,258,592 (GRCm38)	splice site	probably benign
IGL02646:Tln2	APN	9	67,255,996 (GRCm38)	missense	probably benign	0.43
IGL02744:Tln2	APN	9	67,229,376 (GRCm38)	nonsense	probably null
IGL02869:Tln2	APN	9	67,221,525 (GRCm38)	splice site	probably benign
IGL02930:Tln2	APN	9	67,393,662 (GRCm38)	nonsense	probably null
IGL03100:Tln2	APN	9	67,295,737 (GRCm38)	missense	probably damaging	1.00
IGL03326:Tln2	APN	9	67,334,257 (GRCm38)	missense	possibly damaging	0.67
Harrier	UTSW	9	67,330,552 (GRCm38)	nonsense	probably null
Marsh	UTSW	9	67,272,654 (GRCm38)	missense	probably benign	0.19
BB008:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
BB018:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
R0047:Tln2	UTSW	9	67,240,672 (GRCm38)	splice site	probably benign
R0047:Tln2	UTSW	9	67,240,672 (GRCm38)	splice site	probably benign
R0107:Tln2	UTSW	9	67,370,706 (GRCm38)	missense	probably damaging	1.00
R0494:Tln2	UTSW	9	67,355,197 (GRCm38)	missense	probably benign	0.22
R0884:Tln2	UTSW	9	67,370,733 (GRCm38)	missense	probably damaging	1.00
R0947:Tln2	UTSW	9	67,295,813 (GRCm38)	missense	probably benign	0.08
R0989:Tln2	UTSW	9	67,229,454 (GRCm38)	missense	probably damaging	1.00
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,258,566 (GRCm38)	missense	probably damaging	0.96
R1486:Tln2	UTSW	9	67,311,839 (GRCm38)	missense	probably damaging	1.00
R1527:Tln2	UTSW	9	67,272,668 (GRCm38)	missense	possibly damaging	0.95
R1584:Tln2	UTSW	9	67,296,414 (GRCm38)	missense	probably damaging	1.00
R1636:Tln2	UTSW	9	67,306,532 (GRCm38)	missense	probably damaging	1.00
R1656:Tln2	UTSW	9	67,227,107 (GRCm38)	missense	possibly damaging	0.81
R1707:Tln2	UTSW	9	67,375,807 (GRCm38)	missense	probably benign	0.00
R1749:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1751:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1767:Tln2	UTSW	9	67,286,514 (GRCm38)	missense	probably benign	0.01
R1815:Tln2	UTSW	9	67,229,423 (GRCm38)	missense	probably damaging	1.00
R1840:Tln2	UTSW	9	67,342,043 (GRCm38)	missense	probably damaging	1.00
R1847:Tln2	UTSW	9	67,362,687 (GRCm38)	nonsense	probably null
R1964:Tln2	UTSW	9	67,342,135 (GRCm38)	missense	probably benign	0.00
R1968:Tln2	UTSW	9	67,255,901 (GRCm38)	missense	probably damaging	1.00
R2036:Tln2	UTSW	9	67,272,704 (GRCm38)	missense	possibly damaging	0.76
R2038:Tln2	UTSW	9	67,397,653 (GRCm38)	start codon destroyed	probably benign	0.01
R2152:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2153:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2154:Tln2	UTSW	9	67,302,560 (GRCm38)	missense	probably damaging	1.00
R2191:Tln2	UTSW	9	67,355,221 (GRCm38)	missense	probably damaging	1.00
R2192:Tln2	UTSW	9	67,355,221 (GRCm38)	missense	probably damaging	1.00
R2201:Tln2	UTSW	9	67,375,757 (GRCm38)	missense	probably damaging	1.00
R3116:Tln2	UTSW	9	67,355,139 (GRCm38)	missense	probably benign	0.10
R3151:Tln2	UTSW	9	67,330,547 (GRCm38)	critical splice donor site	probably null
R3795:Tln2	UTSW	9	67,255,915 (GRCm38)	missense	probably damaging	0.97
R3953:Tln2	UTSW	9	67,370,629 (GRCm38)	missense	probably damaging	1.00
R4450:Tln2	UTSW	9	67,344,065 (GRCm38)	critical splice donor site	probably null
R4685:Tln2	UTSW	9	67,302,572 (GRCm38)	missense	probably damaging	1.00
R4688:Tln2	UTSW	9	67,397,653 (GRCm38)	start codon destroyed	probably benign	0.01
R4696:Tln2	UTSW	9	67,395,461 (GRCm38)	missense	probably damaging	1.00
R4697:Tln2	UTSW	9	67,395,461 (GRCm38)	missense	probably damaging	1.00
R4700:Tln2	UTSW	9	67,346,527 (GRCm38)	missense	probably benign	0.03
R4701:Tln2	UTSW	9	67,346,527 (GRCm38)	missense	probably benign	0.03
R4741:Tln2	UTSW	9	67,386,555 (GRCm38)	critical splice donor site	probably null
R4806:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4807:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4808:Tln2	UTSW	9	67,331,733 (GRCm38)	missense	probably benign	0.29
R4967:Tln2	UTSW	9	67,355,125 (GRCm38)	missense	probably damaging	0.97
R5061:Tln2	UTSW	9	67,354,468 (GRCm38)	missense	probably benign
R5092:Tln2	UTSW	9	67,256,028 (GRCm38)	missense	probably benign	0.13
R5093:Tln2	UTSW	9	67,334,314 (GRCm38)	missense	probably benign	0.44
R5126:Tln2	UTSW	9	67,258,535 (GRCm38)	missense	probably damaging	1.00
R5204:Tln2	UTSW	9	67,354,482 (GRCm38)	missense	probably benign	0.00
R5236:Tln2	UTSW	9	67,365,923 (GRCm38)	missense	probably damaging	0.99
R5287:Tln2	UTSW	9	67,242,359 (GRCm38)	missense	probably damaging	1.00
R5568:Tln2	UTSW	9	67,311,865 (GRCm38)	missense	probably damaging	1.00
R5571:Tln2	UTSW	9	67,334,320 (GRCm38)	missense	possibly damaging	0.88
R5642:Tln2	UTSW	9	67,296,358 (GRCm38)	missense	probably benign	0.01
R5711:Tln2	UTSW	9	67,392,547 (GRCm38)	missense	probably benign	0.00
R5776:Tln2	UTSW	9	67,258,250 (GRCm38)	missense	probably damaging	1.00
R5791:Tln2	UTSW	9	67,386,605 (GRCm38)	missense	probably damaging	0.98
R5866:Tln2	UTSW	9	67,266,868 (GRCm38)	missense	probably damaging	1.00
R5888:Tln2	UTSW	9	67,229,403 (GRCm38)	missense	probably damaging	1.00
R5902:Tln2	UTSW	9	67,362,717 (GRCm38)	missense	probably benign	0.02
R6106:Tln2	UTSW	9	67,323,020 (GRCm38)	missense	probably damaging	0.99
R6175:Tln2	UTSW	9	67,224,081 (GRCm38)	missense	probably damaging	1.00
R6385:Tln2	UTSW	9	67,278,129 (GRCm38)	missense	probably benign	0.45
R6430:Tln2	UTSW	9	67,272,665 (GRCm38)	missense	probably damaging	1.00
R6441:Tln2	UTSW	9	67,272,689 (GRCm38)	missense	probably damaging	1.00
R6738:Tln2	UTSW	9	67,386,664 (GRCm38)	missense	possibly damaging	0.91
R6776:Tln2	UTSW	9	67,262,905 (GRCm38)	missense	probably damaging	1.00
R6794:Tln2	UTSW	9	67,286,558 (GRCm38)	missense	probably benign	0.07
R6850:Tln2	UTSW	9	67,258,535 (GRCm38)	missense	probably damaging	1.00
R6907:Tln2	UTSW	9	67,397,635 (GRCm38)	missense	probably damaging	0.98
R6909:Tln2	UTSW	9	67,392,532 (GRCm38)	missense	probably damaging	0.97
R6951:Tln2	UTSW	9	67,258,485 (GRCm38)	missense	probably damaging	0.97
R7015:Tln2	UTSW	9	67,362,647 (GRCm38)	missense	possibly damaging	0.55
R7051:Tln2	UTSW	9	67,346,417 (GRCm38)	missense	probably benign	0.00
R7246:Tln2	UTSW	9	67,262,979 (GRCm38)	missense	probably damaging	1.00
R7292:Tln2	UTSW	9	67,346,461 (GRCm38)	missense	probably benign
R7753:Tln2	UTSW	9	67,395,473 (GRCm38)	missense	probably damaging	1.00
R7868:Tln2	UTSW	9	67,348,226 (GRCm38)	missense	probably damaging	1.00
R7931:Tln2	UTSW	9	67,258,460 (GRCm38)	critical splice donor site	probably null
R8023:Tln2	UTSW	9	67,224,064 (GRCm38)	missense	probably damaging	1.00
R8081:Tln2	UTSW	9	67,356,747 (GRCm38)	missense	probably damaging	1.00
R8164:Tln2	UTSW	9	67,319,420 (GRCm38)	missense	probably benign	0.31
R8192:Tln2	UTSW	9	67,346,529 (GRCm38)	nonsense	probably null
R8495:Tln2	UTSW	9	67,354,467 (GRCm38)	missense	probably benign	0.01
R8734:Tln2	UTSW	9	67,272,654 (GRCm38)	missense	probably benign	0.19
R8739:Tln2	UTSW	9	67,258,273 (GRCm38)	missense	probably damaging	1.00
R8757:Tln2	UTSW	9	67,367,218 (GRCm38)	missense	probably damaging	1.00
R8759:Tln2	UTSW	9	67,367,218 (GRCm38)	missense	probably damaging	1.00
R8770:Tln2	UTSW	9	67,323,022 (GRCm38)	missense	probably benign
R8781:Tln2	UTSW	9	67,255,951 (GRCm38)	missense	probably damaging	1.00
R8812:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8814:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8816:Tln2	UTSW	9	67,221,517 (GRCm38)	missense	probably damaging	1.00
R8816:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8833:Tln2	UTSW	9	67,221,411 (GRCm38)	missense	possibly damaging	0.75
R8835:Tln2	UTSW	9	67,397,693 (GRCm38)	splice site	probably benign
R8837:Tln2	UTSW	9	67,250,584 (GRCm38)	missense	probably damaging	0.99
R8843:Tln2	UTSW	9	67,395,545 (GRCm38)	missense	probably damaging	1.00
R8864:Tln2	UTSW	9	67,330,552 (GRCm38)	nonsense	probably null
R8867:Tln2	UTSW	9	67,330,550 (GRCm38)	missense	probably damaging	0.98
R8921:Tln2	UTSW	9	67,266,823 (GRCm38)	missense	probably damaging	0.99
R9080:Tln2	UTSW	9	67,346,561 (GRCm38)	missense	probably damaging	1.00
R9083:Tln2	UTSW	9	67,362,645 (GRCm38)	missense	probably damaging	0.96
R9150:Tln2	UTSW	9	67,221,496 (GRCm38)	missense	probably damaging	1.00
R9287:Tln2	UTSW	9	67,370,698 (GRCm38)	missense	probably benign	0.20
R9330:Tln2	UTSW	9	67,321,931 (GRCm38)	missense	possibly damaging	0.61
R9343:Tln2	UTSW	9	67,323,071 (GRCm38)	missense	probably benign	0.10
R9355:Tln2	UTSW	9	67,355,247 (GRCm38)	missense	possibly damaging	0.46
R9383:Tln2	UTSW	9	67,370,761 (GRCm38)	missense	probably benign	0.17
R9386:Tln2	UTSW	9	67,365,967 (GRCm38)	missense	possibly damaging	0.78
R9407:Tln2	UTSW	9	67,229,450 (GRCm38)	missense	probably damaging	1.00
R9483:Tln2	UTSW	9	67,392,487 (GRCm38)	missense	probably damaging	1.00
R9523:Tln2	UTSW	9	67,258,484 (GRCm38)	missense	probably damaging	0.99
R9642:Tln2	UTSW	9	67,250,544 (GRCm38)	missense	probably benign	0.02
R9703:Tln2	UTSW	9	67,386,656 (GRCm38)	missense	probably damaging	1.00
X0027:Tln2	UTSW	9	67,376,853 (GRCm38)	missense	probably damaging	1.00
X0064:Tln2	UTSW	9	67,348,138 (GRCm38)	missense	probably damaging	1.00
X0067:Tln2	UTSW	9	67,370,691 (GRCm38)	missense	probably damaging	1.00
Z1176:Tln2	UTSW	9	67,346,485 (GRCm38)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- CAGGCACCCAGTAAGTATATCAGCTTC -3'
(R):5'- ACGCCTTTTCCAATGAGTCATGTCATC -3'

Sequencing Primer
(F):5'- GGAGAACTTGTGAATAACCCTTACC -3'
(R):5'- CAATGAGTCATGTCATCCTTGC -3'

Posted On

2014-05-23