Incidental Mutation 'R1753:Cdc16'
ID 193742
Institutional Source Beutler Lab
Gene Symbol Cdc16
Ensembl Gene ENSMUSG00000038416
Gene Name CDC16 cell division cycle 16
Synonyms 2810431D22Rik, 2700071J12Rik
MMRRC Submission 039785-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.970) question?
Stock # R1753 (G1)
Quality Score 225
Status Not validated
Chromosome 8
Chromosomal Location 13757676-13781938 bp(+) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) C to A at 13764688 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Stop codon at position 157 (Y157*)
Ref Sequence ENSEMBL: ENSMUSP00000147399 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000043962] [ENSMUST00000130173] [ENSMUST00000134645]
AlphaFold Q8R349
Predicted Effect probably null
Transcript: ENSMUST00000043962
AA Change: Y251*
SMART Domains Protein: ENSMUSP00000047950
Gene: ENSMUSG00000038416
AA Change: Y251*

Pfam:TPR_9 11 63 1.8e-3 PFAM
Pfam:ANAPC3 15 95 3.5e-23 PFAM
TPR 130 163 1.17e1 SMART
Blast:TPR 299 333 2e-8 BLAST
Blast:TPR 334 367 1e-14 BLAST
TPR 368 401 1.48e1 SMART
Blast:TPR 402 435 7e-15 BLAST
TPR 445 478 6.68e-6 SMART
TPR 479 512 1.74e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000129872
Predicted Effect probably null
Transcript: ENSMUST00000130173
AA Change: Y157*
Predicted Effect probably benign
Transcript: ENSMUST00000134645
Coding Region Coverage
  • 1x: 97.5%
  • 3x: 96.9%
  • 10x: 95.3%
  • 20x: 92.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. [provided by RefSeq, Jan 2016]
Allele List at MGI
Other mutations in this stock
Total: 102 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca8b A T 11: 109,973,716 (GRCm38) F409I probably benign Het
Adamts19 A C 18: 59,007,372 (GRCm38) I848L possibly damaging Het
Adamts5 T C 16: 85,899,352 (GRCm38) S306G probably damaging Het
Adamts8 T G 9: 30,954,614 (GRCm38) I486S probably benign Het
Adgrg5 T C 8: 94,942,052 (GRCm38) F499L possibly damaging Het
Akr1c21 T A 13: 4,577,135 (GRCm38) C145* probably null Het
Anp32b T G 4: 46,460,241 (GRCm38) probably null Het
Arhgap31 A G 16: 38,601,612 (GRCm38) V1364A possibly damaging Het
C2cd6 T C 1: 59,094,833 (GRCm38) R10G possibly damaging Het
Cacna2d1 A G 5: 16,302,354 (GRCm38) E367G possibly damaging Het
Ccdc81 C T 7: 89,866,561 (GRCm38) E637K probably benign Het
Cd2 A T 3: 101,287,499 (GRCm38) M91K possibly damaging Het
Celsr3 G T 9: 108,831,857 (GRCm38) V1301F probably damaging Het
Cep164 C T 9: 45,792,937 (GRCm38) G961S probably damaging Het
Cep290 T C 10: 100,513,981 (GRCm38) V630A probably benign Het
Chd5 C T 4: 152,378,815 (GRCm38) S1451F probably damaging Het
Cldn23 A C 8: 35,825,986 (GRCm38) L116R possibly damaging Het
Cngb1 A T 8: 95,297,773 (GRCm38) probably benign Het
Cpb1 G T 3: 20,266,241 (GRCm38) N151K possibly damaging Het
Cpn2 A G 16: 30,260,100 (GRCm38) F261S probably damaging Het
Crlf3 A C 11: 80,057,872 (GRCm38) V249G probably damaging Het
Csmd1 A T 8: 16,157,120 (GRCm38) Y1298* probably null Het
Cstf2t C A 19: 31,083,685 (GRCm38) P207Q possibly damaging Het
Cym A C 3: 107,213,425 (GRCm38) L288R possibly damaging Het
Cyp2j12 C T 4: 96,121,432 (GRCm38) probably null Het
Dlx6 C T 6: 6,863,665 (GRCm38) Q96* probably null Het
Dnah7b T C 1: 46,322,335 (GRCm38) F3465S probably damaging Het
Dnmt3a A T 12: 3,873,342 (GRCm38) M181L possibly damaging Het
Duox1 T A 2: 122,333,429 (GRCm38) M859K probably damaging Het
Eif2b4 A T 5: 31,192,940 (GRCm38) S13T probably benign Het
Ercc6 T C 14: 32,576,999 (GRCm38) V1448A probably benign Het
Esp24 A G 17: 39,040,002 (GRCm38) E31G possibly damaging Het
F2rl2 T A 13: 95,701,461 (GRCm38) M338K probably benign Het
Fgfbp1 A C 5: 43,979,923 (GRCm38) L9R possibly damaging Het
Gal3st2b A T 1: 93,940,616 (GRCm38) N188Y probably damaging Het
Gpr179 G C 11: 97,346,578 (GRCm38) C372W probably damaging Het
Grn T G 11: 102,433,267 (GRCm38) C61G probably damaging Het
Herc1 T C 9: 66,502,084 (GRCm38) probably null Het
Herc1 T G 9: 66,469,010 (GRCm38) C3371G probably damaging Het
Hmcn1 C T 1: 150,586,468 (GRCm38) G5153D possibly damaging Het
Ifi35 A T 11: 101,456,635 (GRCm38) R31W probably damaging Het
Ifit1bl1 T A 19: 34,593,860 (GRCm38) H399L probably benign Het
Irx3 G A 8: 91,800,734 (GRCm38) P114L probably damaging Het
Kat6a T A 8: 22,935,797 (GRCm38) D1119E probably benign Het
Kmt2d G T 15: 98,843,482 (GRCm38) probably benign Het
Kng1 A T 16: 23,079,119 (GRCm38) H423L possibly damaging Het
Lrp2 T A 2: 69,496,489 (GRCm38) Q1746L possibly damaging Het
Lrrc29 A T 8: 105,313,192 (GRCm38) V517E probably damaging Het
Lrrc63 A T 14: 75,086,344 (GRCm38) probably null Het
Mad2l1 T A 6: 66,539,813 (GRCm38) V163E possibly damaging Het
Map3k19 A G 1: 127,822,680 (GRCm38) M978T probably benign Het
Mon1a A C 9: 107,901,363 (GRCm38) N262T probably damaging Het
Mpo A G 11: 87,795,881 (GRCm38) N85D probably benign Het
Mpp4 T C 1: 59,144,810 (GRCm38) D244G probably null Het
Mstn A G 1: 53,066,558 (GRCm38) Y353C probably damaging Het
Mx1 T A 16: 97,454,158 (GRCm38) N232Y probably damaging Het
Mycs T C X: 5,468,103 (GRCm38) R308G probably benign Het
Myh11 A T 16: 14,277,870 (GRCm38) D9E probably benign Het
Nfe2l3 A T 6: 51,433,412 (GRCm38) Q169L probably null Het
Nr5a1 G T 2: 38,708,419 (GRCm38) T122N possibly damaging Het
Nras A G 3: 103,058,979 (GRCm38) T20A probably damaging Het
Obp2b T A 2: 25,738,640 (GRCm38) probably null Het
Olfr1109 T C 2: 87,093,227 (GRCm38) T57A probably damaging Het
Olfr1143 T A 2: 87,802,762 (GRCm38) F124L probably benign Het
Olfr1410 G A 1: 92,608,400 (GRCm38) V188M probably benign Het
Olfr365 A G 2: 37,201,427 (GRCm38) Y62C probably damaging Het
Olfr577 T C 7: 102,973,056 (GRCm38) N312S probably benign Het
Pcdh17 A T 14: 84,477,654 (GRCm38) T920S probably benign Het
Pcdh9 T C 14: 93,887,225 (GRCm38) D503G probably benign Het
Pcdhb12 C T 18: 37,436,671 (GRCm38) T290I probably damaging Het
Pde6b C A 5: 108,388,691 (GRCm38) C84* probably null Het
Pex1 A T 5: 3,630,044 (GRCm38) N914I probably damaging Het
Pign A G 1: 105,589,317 (GRCm38) V528A possibly damaging Het
Pkhd1 T G 1: 20,533,905 (GRCm38) D1187A possibly damaging Het
Ppip5k1 T C 2: 121,342,631 (GRCm38) K489E probably damaging Het
Prob1 T C 18: 35,653,252 (GRCm38) T650A possibly damaging Het
Radil T C 5: 142,495,336 (GRCm38) Y572C probably damaging Het
Rapgef2 A G 3: 79,088,791 (GRCm38) I555T possibly damaging Het
Rgs5 G A 1: 169,682,817 (GRCm38) probably null Het
Rnaset2b G A 17: 6,981,107 (GRCm38) probably null Het
S1pr1 A G 3: 115,711,938 (GRCm38) S336P probably benign Het
Slc24a5 A G 2: 125,083,195 (GRCm38) E252G possibly damaging Het
Slc34a2 A T 5: 53,061,391 (GRCm38) I184F probably benign Het
Slc35a3 A G 3: 116,677,948 (GRCm38) V224A possibly damaging Het
Slc39a9 A G 12: 80,677,202 (GRCm38) H211R probably damaging Het
Slu7 A G 11: 43,439,268 (GRCm38) N174S probably benign Het
Smarcd3 A T 5: 24,595,822 (GRCm38) Y131* probably null Het
Syne1 A G 10: 5,367,621 (GRCm38) M491T probably benign Het
Tars G A 15: 11,394,243 (GRCm38) Q103* probably null Het
Tmem132d C A 5: 127,789,855 (GRCm38) E660D probably benign Het
Ttn T C 2: 76,812,972 (GRCm38) E13201G probably damaging Het
Ttn T A 2: 76,752,261 (GRCm38) M22763L probably benign Het
Ttn T C 2: 76,745,043 (GRCm38) T16842A probably damaging Het
Ubp1 A T 9: 113,955,969 (GRCm38) I117L possibly damaging Het
Usp24 T A 4: 106,377,559 (GRCm38) H954Q probably benign Het
Vmn1r174 G A 7: 23,754,197 (GRCm38) R96H probably benign Het
Vmn2r63 G A 7: 42,928,245 (GRCm38) Q290* probably null Het
Vnn3 T C 10: 23,865,820 (GRCm38) I341T probably benign Het
Wbp2nl A G 15: 82,305,744 (GRCm38) T46A probably damaging Het
Wdr48 G T 9: 119,924,247 (GRCm38) E625* probably null Het
Wdr6 C T 9: 108,575,164 (GRCm38) V507I probably damaging Het
Zp2 A T 7: 120,138,105 (GRCm38) W287R probably benign Het
Other mutations in Cdc16
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00850:Cdc16 APN 8 13,767,575 (GRCm38) nonsense probably null
IGL01109:Cdc16 APN 8 13,764,606 (GRCm38) missense probably benign 0.00
IGL01475:Cdc16 APN 8 13,781,542 (GRCm38) missense probably benign
IGL02729:Cdc16 APN 8 13,779,250 (GRCm38) missense possibly damaging 0.93
IGL03389:Cdc16 APN 8 13,759,179 (GRCm38) missense probably damaging 1.00
R0026:Cdc16 UTSW 8 13,759,130 (GRCm38) splice site probably null
R0373:Cdc16 UTSW 8 13,779,264 (GRCm38) missense probably benign 0.04
R0520:Cdc16 UTSW 8 13,760,569 (GRCm38) critical splice donor site probably null
R0564:Cdc16 UTSW 8 13,781,618 (GRCm38) missense probably damaging 1.00
R1470:Cdc16 UTSW 8 13,758,992 (GRCm38) splice site probably benign
R1487:Cdc16 UTSW 8 13,771,445 (GRCm38) missense probably benign 0.17
R1883:Cdc16 UTSW 8 13,775,738 (GRCm38) missense probably damaging 1.00
R3087:Cdc16 UTSW 8 13,759,004 (GRCm38) missense probably damaging 0.98
R3418:Cdc16 UTSW 8 13,769,489 (GRCm38) nonsense probably null
R3756:Cdc16 UTSW 8 13,777,609 (GRCm38) critical splice donor site probably null
R4152:Cdc16 UTSW 8 13,762,857 (GRCm38) missense probably damaging 1.00
R4842:Cdc16 UTSW 8 13,781,644 (GRCm38) utr 3 prime probably benign
R5122:Cdc16 UTSW 8 13,764,570 (GRCm38) missense probably damaging 1.00
R5492:Cdc16 UTSW 8 13,763,915 (GRCm38) splice site probably null
R5982:Cdc16 UTSW 8 13,781,399 (GRCm38) missense possibly damaging 0.73
R6145:Cdc16 UTSW 8 13,767,573 (GRCm38) missense possibly damaging 0.96
R6154:Cdc16 UTSW 8 13,768,609 (GRCm38) missense possibly damaging 0.87
R6611:Cdc16 UTSW 8 13,781,512 (GRCm38) missense probably benign
R6992:Cdc16 UTSW 8 13,759,188 (GRCm38) missense probably benign 0.22
R7011:Cdc16 UTSW 8 13,769,451 (GRCm38) missense probably damaging 1.00
R7484:Cdc16 UTSW 8 13,777,605 (GRCm38) missense probably benign 0.01
R7593:Cdc16 UTSW 8 13,777,605 (GRCm38) missense probably benign 0.01
R7946:Cdc16 UTSW 8 13,762,882 (GRCm38) missense probably benign 0.22
R9019:Cdc16 UTSW 8 13,781,501 (GRCm38) missense probably benign
R9655:Cdc16 UTSW 8 13,759,153 (GRCm38) missense possibly damaging 0.93
R9668:Cdc16 UTSW 8 13,767,552 (GRCm38) missense possibly damaging 0.94
R9796:Cdc16 UTSW 8 13,757,693 (GRCm38) start gained probably benign
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2014-05-23