Mutagenetix > Incidental Mutation

Incidental Mutation 'R1768:Olfr676'

194583

Institutional Source

Beutler Lab

Gene Symbol

Olfr676

Ensembl Gene

ENSMUSG00000073915

Gene Name

olfactory receptor 676

Synonyms

MOR32-1, GA_x6K02T2PBJ9-7664016-7664969

MMRRC Submission

039799-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.083) question?

Stock #

R1768 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

105032698-105037750 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 105035950 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 251 (S251G)

Ref Sequence

ENSEMBL: ENSMUSP00000151474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098162] [ENSMUST00000219602]

AlphaFold

Q8VGZ9

Predicted Effect

probably benign
Transcript: ENSMUST00000098162
AA Change: S251G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000095765
Gene: ENSMUSG00000073915
AA Change: S251G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	41	317	3e-113	PFAM
Pfam:7TM_GPCR_Srsx	45	316	4.5e-7	PFAM
Pfam:7tm_1	51	301	1.5e-15	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215533

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219042

Predicted Effect

probably benign
Transcript: ENSMUST00000219602
AA Change: S251G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 97.4%
3x: 96.9%
10x: 95.3%
20x: 92.4%

Validation Efficiency

91% (107/117)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 112 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921524L21Rik	G	C	18: 6,623,470 (GRCm38)	R60P	possibly damaging	Het
Aox2	T	A	1: 58,354,195 (GRCm38)	C1199S	probably benign	Het
Arhgap18	G	A	10: 26,887,861 (GRCm38)	M482I	probably damaging	Het
Arhgap18	G	T	10: 26,887,862 (GRCm38)	A483S	probably damaging	Het
Arid4a	G	A	12: 71,075,338 (GRCm38)	S509N	probably benign	Het
BC051019	T	C	7: 109,723,174 (GRCm38)	T38A	probably benign	Het
Bcam	T	C	7: 19,765,618 (GRCm38)	N192S	probably null	Het
Bend5	A	T	4: 111,454,241 (GRCm38)	K351*	probably null	Het
Bicdl2	T	A	17: 23,665,949 (GRCm38)	M208K	probably damaging	Het
Ccdc155	T	A	7: 45,188,803 (GRCm38)		probably null	Het
Ccp110	A	T	7: 118,726,024 (GRCm38)		probably null	Het
Cdc6	A	T	11: 98,912,217 (GRCm38)	T328S	probably damaging	Het
Cdk5rap2	G	T	4: 70,307,233 (GRCm38)	N558K	probably benign	Het
Cdkn1c	C	T	7: 143,459,121 (GRCm38)	R146K	probably benign	Het
Ceacam18	G	A	7: 43,648,494 (GRCm38)	C371Y	probably benign	Het
Cep95	T	A	11: 106,806,351 (GRCm38)	C233*	probably null	Het
Chrnd	A	G	1: 87,194,928 (GRCm38)	I144V	probably benign	Het
Col6a4	T	A	9: 106,080,100 (GRCm38)	Q175L	probably benign	Het
Cym	A	T	3: 107,213,500 (GRCm38)	V263E	probably damaging	Het
Cyp2a4	G	T	7: 26,312,772 (GRCm38)	V327F	possibly damaging	Het
Dhx29	T	A	13: 112,948,240 (GRCm38)	M664K	probably damaging	Het
Dlec1	T	G	9: 119,146,007 (GRCm38)		probably null	Het
Dna2	T	C	10: 62,957,084 (GRCm38)	Y293H	probably benign	Het
Dnase1l3	T	A	14: 7,974,104 (GRCm38)	N196Y	probably damaging	Het
Eea1	T	A	10: 95,996,960 (GRCm38)	D222E	probably damaging	Het
Efcab14	A	T	4: 115,752,919 (GRCm38)		probably null	Het
Entpd5	C	T	12: 84,386,211 (GRCm38)	R189H	probably benign	Het
Exoc4	A	T	6: 33,758,050 (GRCm38)	K534M	probably damaging	Het
Extl1	T	A	4: 134,371,138 (GRCm38)	Y194F	probably benign	Het
Eya1	A	G	1: 14,253,075 (GRCm38)	L161S	possibly damaging	Het
Fam163b	T	C	2: 27,112,862 (GRCm38)	E41G	possibly damaging	Het
Fam180a	A	C	6: 35,315,352 (GRCm38)	S40A	probably benign	Het
Fbxl4	T	C	4: 22,385,950 (GRCm38)	S186P	probably benign	Het
Fbxw19	A	T	9: 109,494,772 (GRCm38)	L45*	probably null	Het
Fgf14	G	T	14: 124,676,512 (GRCm38)	T69N	probably benign	Het
Flt1	A	G	5: 147,672,709 (GRCm38)	Y432H	probably damaging	Het
Frmd4b	A	T	6: 97,306,764 (GRCm38)	L374Q	possibly damaging	Het
G6pc2	T	A	2: 69,222,977 (GRCm38)	V125D	probably damaging	Het
Gna15	A	T	10: 81,512,120 (GRCm38)	L164Q	probably damaging	Het
Gnaz	C	A	10: 74,991,870 (GRCm38)	D151E	possibly damaging	Het
Has1	T	C	17: 17,850,300 (GRCm38)	T120A	probably benign	Het
Hectd4	T	A	5: 121,358,303 (GRCm38)	D3919E	possibly damaging	Het
Hs3st5	A	G	10: 36,833,169 (GRCm38)	I233M	probably benign	Het
Ilf3	T	C	9: 21,403,142 (GRCm38)		probably benign	Het
Inpp5b	T	A	4: 124,793,276 (GRCm38)	L765*	probably null	Het
Insr	A	T	8: 3,159,561 (GRCm38)	I1174N	probably damaging	Het
Kcnq3	A	G	15: 66,005,906 (GRCm38)	L445P	probably damaging	Het
Kctd20	A	T	17: 28,962,850 (GRCm38)	N159Y	probably damaging	Het
Kctd20	A	T	17: 28,966,781 (GRCm38)	D366V	probably damaging	Het
Klk15	T	C	7: 43,938,333 (GRCm38)		probably benign	Het
Lama4	T	G	10: 39,103,501 (GRCm38)	N1658K	possibly damaging	Het
Lonrf2	G	A	1: 38,813,276 (GRCm38)	P165S	probably benign	Het
Mas1	T	C	17: 12,841,699 (GRCm38)	Y279C	probably damaging	Het
Mast2	G	T	4: 116,306,959 (GRCm38)	D1747E	probably damaging	Het
Mest	G	A	6: 30,745,139 (GRCm38)	M235I	probably benign	Het
Mfsd6	A	G	1: 52,660,805 (GRCm38)		probably null	Het
Mllt10	T	A	2: 18,162,846 (GRCm38)	S449R	probably damaging	Het
Mon2	T	A	10: 123,013,763 (GRCm38)	T1211S	probably benign	Het
Mrnip	G	A	11: 50,176,861 (GRCm38)	C27Y	probably damaging	Het
Myh15	A	T	16: 49,163,135 (GRCm38)	T1538S	probably benign	Het
Nfatc2ip	A	G	7: 126,390,462 (GRCm38)	V250A	probably benign	Het
Npy1r	A	G	8: 66,704,525 (GRCm38)	D199G	possibly damaging	Het
Numbl	A	G	7: 27,280,954 (GRCm38)	T454A	probably benign	Het
Nutm2	T	G	13: 50,473,116 (GRCm38)	F436V	probably damaging	Het
Olfr1112	T	A	2: 87,191,698 (GRCm38)	S4T	probably benign	Het
Olfr330	A	G	11: 58,529,776 (GRCm38)	L70P	probably damaging	Het
Olfr458	A	G	6: 42,460,677 (GRCm38)	L114S	probably damaging	Het
Olfr556	A	G	7: 102,670,301 (GRCm38)	Y127C	probably damaging	Het
Olfr631	C	T	7: 103,929,725 (GRCm38)	R301*	probably null	Het
Olfr64	A	G	7: 103,893,277 (GRCm38)	S153P	probably benign	Het
Olfr707	G	T	7: 106,891,978 (GRCm38)	L44M	probably damaging	Het
Olfr707	A	T	7: 106,891,977 (GRCm38)		probably null	Het
Olfr876	T	A	9: 37,804,303 (GRCm38)	Y131N	probably damaging	Het
Opa1	T	C	16: 29,620,810 (GRCm38)	S773P	probably benign	Het
Pde8a	G	C	7: 81,300,723 (GRCm38)		probably null	Het
Pgam1	T	C	19: 41,917,705 (GRCm38)	F232S	probably damaging	Het
Pgk1	T	A	X: 106,200,308 (GRCm38)	V303E	possibly damaging	Het
Pirb	A	T	7: 3,717,190 (GRCm38)	C395S	probably damaging	Het
Plxnc1	C	T	10: 94,844,322 (GRCm38)	V824I	probably benign	Het
Ppp6r1	A	G	7: 4,633,692 (GRCm38)		probably null	Het
Rapgef4	C	A	2: 72,225,787 (GRCm38)		probably benign	Het
Rars	T	A	11: 35,809,638 (GRCm38)	T539S	probably damaging	Het
Rbm44	G	T	1: 91,153,957 (GRCm38)		probably null	Het
RP23-114B10.6	T	C	8: 69,373,558 (GRCm38)	I119M	unknown	Het
Samd4b	A	G	7: 28,413,892 (GRCm38)	I216T	probably benign	Het
Serpine2	A	C	1: 79,816,815 (GRCm38)	F134V	probably damaging	Het
Shmt1	A	G	11: 60,792,964 (GRCm38)	Y341H	probably damaging	Het
Slc23a2	C	A	2: 132,075,641 (GRCm38)	V226F	probably benign	Het
Slc23a4	A	G	6: 34,956,961 (GRCm38)	I69T	probably damaging	Het
Slc37a1	C	A	17: 31,333,678 (GRCm38)	T319K	possibly damaging	Het
Slc6a4	A	T	11: 77,013,252 (GRCm38)	T178S	probably damaging	Het
Smarca4	G	A	9: 21,701,183 (GRCm38)	A1588T	possibly damaging	Het
Spag17	A	T	3: 100,027,352 (GRCm38)	Y650F	possibly damaging	Het
Stab2	C	T	10: 87,003,008 (GRCm38)	G65S	probably damaging	Het
Stambpl1	C	G	19: 34,226,721 (GRCm38)	N70K	probably damaging	Het
Stip1	C	A	19: 7,021,797 (GRCm38)	C471F	probably damaging	Het
Taf1	T	C	X: 101,540,894 (GRCm38)	S223P	probably benign	Het
Tchh	C	A	3: 93,443,575 (GRCm38)	N107K	possibly damaging	Het
Tenm3	A	C	8: 48,232,104 (GRCm38)	H2432Q	probably damaging	Het
Tmem243	A	G	5: 9,118,548 (GRCm38)	N110S	probably damaging	Het
Toe1	A	T	4: 116,804,879 (GRCm38)	I306F	probably benign	Het
Trank1	G	A	9: 111,392,927 (GRCm38)	V2911M	probably damaging	Het
Trpm4	A	G	7: 45,308,612 (GRCm38)	I811T	probably damaging	Het
Tspear	T	A	10: 77,875,116 (GRCm38)		probably null	Het
Ttc28	A	T	5: 111,277,168 (GRCm38)	I1589F	possibly damaging	Het
Tubgcp3	A	G	8: 12,649,686 (GRCm38)		probably benign	Het
U2af2	C	A	7: 5,067,545 (GRCm38)	R78S	probably benign	Het
Wdr17	A	T	8: 54,673,654 (GRCm38)	D388E	possibly damaging	Het
Wdr3	A	T	3: 100,153,870 (GRCm38)	S261T	probably benign	Het
Zfp667	A	G	7: 6,305,067 (GRCm38)	N245D	possibly damaging	Het
Zfp692	A	G	11: 58,310,176 (GRCm38)		probably benign	Het
Zfp729a	T	C	13: 67,619,251 (GRCm38)	H953R	probably benign	Het

Other mutations in Olfr676

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02034:Olfr676	APN	7	105,035,390 (GRCm38)	missense	probably benign
IGL02244:Olfr676	APN	7	105,035,945 (GRCm38)	missense	probably damaging	1.00
IGL03493:Olfr676	APN	7	105,035,944 (GRCm38)	missense	probably damaging	1.00
R1181:Olfr676	UTSW	7	105,035,814 (GRCm38)	missense	probably damaging	1.00
R1223:Olfr676	UTSW	7	105,035,566 (GRCm38)	missense	probably benign	0.01
R1460:Olfr676	UTSW	7	105,035,708 (GRCm38)	missense	possibly damaging	0.80
R2256:Olfr676	UTSW	7	105,035,819 (GRCm38)	missense	probably benign	0.00
R2257:Olfr676	UTSW	7	105,035,819 (GRCm38)	missense	probably benign	0.00
R3846:Olfr676	UTSW	7	105,035,689 (GRCm38)	missense	probably benign	0.40
R4486:Olfr676	UTSW	7	105,035,303 (GRCm38)	missense	probably benign	0.00
R4488:Olfr676	UTSW	7	105,035,303 (GRCm38)	missense	probably benign	0.00
R4489:Olfr676	UTSW	7	105,035,303 (GRCm38)	missense	probably benign	0.00
R4598:Olfr676	UTSW	7	105,036,073 (GRCm38)	missense	probably benign	0.19
R4599:Olfr676	UTSW	7	105,036,073 (GRCm38)	missense	probably benign	0.00
R4701:Olfr676	UTSW	7	105,035,591 (GRCm38)	missense	probably damaging	1.00
R4855:Olfr676	UTSW	7	105,035,651 (GRCm38)	missense	probably damaging	0.99
R5083:Olfr676	UTSW	7	105,035,411 (GRCm38)	missense	probably damaging	1.00
R5743:Olfr676	UTSW	7	105,036,156 (GRCm38)	splice site	probably null
R5798:Olfr676	UTSW	7	105,036,137 (GRCm38)	missense	probably benign	0.00
R6279:Olfr676	UTSW	7	105,035,671 (GRCm38)	missense	probably benign	0.25
R6300:Olfr676	UTSW	7	105,035,671 (GRCm38)	missense	probably benign	0.25
R7400:Olfr676	UTSW	7	105,035,210 (GRCm38)	missense	probably benign
R7672:Olfr676	UTSW	7	105,035,543 (GRCm38)	missense	probably damaging	1.00
R7698:Olfr676	UTSW	7	105,035,907 (GRCm38)	missense	probably benign	0.01
R8468:Olfr676	UTSW	7	105,035,746 (GRCm38)	missense	probably damaging	0.97
R9297:Olfr676	UTSW	7	105,035,623 (GRCm38)	missense	probably damaging	1.00
R9318:Olfr676	UTSW	7	105,035,623 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGTGCCAGCATCAGAGCCAATATC -3'
(R):5'- AGCAGAAGGCTCCTCTAGTAGTTCG -3'

Sequencing Primer
(F):5'- CAGAGCCAATATCTACTTTGGTCTTG -3'
(R):5'- GGGCATCATCTGTGATCAATC -3'

Posted On

2014-05-23